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The map label for this gene is purK

Identifier: 15605791

GI number: 15605791

Start: 156794

End: 157891

Strand: Direct

Name: purK

Synonym: aq_245

Alternate gene names: 15605791

Gene position: 156794-157891 (Clockwise)

Preceding gene: 15605788

Following gene: 15605798

Centisome position: 10.11

GC content: 41.53

Gene sequence:

>1098_bases
ATGCTTACCGTGGGTATCCTCGGAGGTGGACAGCTCGGATGGATGACTATACTGGAAGGGAGAAAACTCGGGTTTAAATT
TCACGTCCTTGAAGACAAAGAAAACGCTCCTGCCTGCAGGGTTGCTGACAGGTGTTTCAGAACAGGACAAATAAGTGAAT
TCGTAGACTCCTGTGACATTATTACTTACGAATTTGAACACATTAAAGATGAAGTTTTGGAAAAGTGCGAGAGTAAATTA
ATTCCCAACCCTCAAGCTCTTTACGTTAAAAAAAGCAGAATCAGGGAAAAGCTTTTTCTGAAAAAGCACGGTTTTCCCGT
GCCGGAGTTTTTAGTAATCAAAAGGGACGAGATAATAGATGCTTTAAAGAGTTTTAAACTTCCTGTGGTGATAAAGGCGG
AAAAGCTTGGCTACGACGGAAAAGGACAGTACAGGATAAAAAAACTCGAGGATGCAAACCAGGTTGTCAAAAACCACGAC
AAAGAAGAAAGTTTCATAATTGAGGAATTCGTAAAGTTTGAAGCGGAAATTTCCTGTATAGGTGTAAGGGACAGGGAAGG
TAAAACGTACTTTTACCCTCAGCCATTCAATAAACACGAAGAGGGTATACTTATTTACAACTACGTGCCCTATGCAAAGT
TAAAAGAAGCTGAAGAAATAACTAAGAGGCTAATGGAGCTTTTAGATATAGTGGGTGTGTTCACGGTTGAATTTTTCCTG
CTAAAAGACGGGAGAGTTTTAATAAACGAATTTGCTCCGAGGGTTCACAACACGGGACACTGGACCTTGGATGGAGCGTA
CACCTCCCAGTTTGAAAACCTCCTTAGGGCTATAACGGAAATGCCTCTTGGTTCTACGGAGTTAAAACTACCGAGCGGTA
TGGTGAATATACTCGGGAAATCTTATGAGGAAATCCCTCTGAAAGAAATCCTTTCCGTTGAAGGTGCAAAACTATACTGG
TATGGCAAAGAGAAGAAACCAAGAAGGAAGGTAGGACACGTTAACGTGGTGGGAAGGTCAAAGGAAGAAGTAGTTGAAAA
AGTAGAAAGAGTCTTCACACTTCTCAAAGGCTCAAGAGAAAAACTCCCAGCACCATGA

Upstream 100 bases:

>100_bases
TTGAAACTTCGGTGATAAAGGAGCATTTTTCACAAAAGAAATCAGTTTCTAATGTACCGTGTGGATAAAAATGATTATTT
GTGTTTAAAATTCTTTAGAA

Downstream 100 bases:

>100_bases
TTATACTCGCCTTAAAAGCGTTCTTAACTTTTTCGCCGAAGAAGACCTTTCCGTAAAGGACGTCAAGGAGTATGGCCAGC
CTTTCGGTGGCGGCAACAAA

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MLTVGILGGGQLGWMTILEGRKLGFKFHVLEDKENAPACRVADRCFRTGQISEFVDSCDIITYEFEHIKDEVLEKCESKL
IPNPQALYVKKSRIREKLFLKKHGFPVPEFLVIKRDEIIDALKSFKLPVVIKAEKLGYDGKGQYRIKKLEDANQVVKNHD
KEESFIIEEFVKFEAEISCIGVRDREGKTYFYPQPFNKHEEGILIYNYVPYAKLKEAEEITKRLMELLDIVGVFTVEFFL
LKDGRVLINEFAPRVHNTGHWTLDGAYTSQFENLLRAITEMPLGSTELKLPSGMVNILGKSYEEIPLKEILSVEGAKLYW
YGKEKKPRRKVGHVNVVGRSKEEVVEKVERVFTLLKGSREKLPAP

Sequences:

>Translated_365_residues
MLTVGILGGGQLGWMTILEGRKLGFKFHVLEDKENAPACRVADRCFRTGQISEFVDSCDIITYEFEHIKDEVLEKCESKL
IPNPQALYVKKSRIREKLFLKKHGFPVPEFLVIKRDEIIDALKSFKLPVVIKAEKLGYDGKGQYRIKKLEDANQVVKNHD
KEESFIIEEFVKFEAEISCIGVRDREGKTYFYPQPFNKHEEGILIYNYVPYAKLKEAEEITKRLMELLDIVGVFTVEFFL
LKDGRVLINEFAPRVHNTGHWTLDGAYTSQFENLLRAITEMPLGSTELKLPSGMVNILGKSYEEIPLKEILSVEGAKLYW
YGKEKKPRRKVGHVNVVGRSKEEVVEKVERVFTLLKGSREKLPAP
>Mature_365_residues
MLTVGILGGGQLGWMTILEGRKLGFKFHVLEDKENAPACRVADRCFRTGQISEFVDSCDIITYEFEHIKDEVLEKCESKL
IPNPQALYVKKSRIREKLFLKKHGFPVPEFLVIKRDEIIDALKSFKLPVVIKAEKLGYDGKGQYRIKKLEDANQVVKNHD
KEESFIIEEFVKFEAEISCIGVRDREGKTYFYPQPFNKHEEGILIYNYVPYAKLKEAEEITKRLMELLDIVGVFTVEFFL
LKDGRVLINEFAPRVHNTGHWTLDGAYTSQFENLLRAITEMPLGSTELKLPSGMVNILGKSYEEIPLKEILSVEGAKLYW
YGKEKKPRRKVGHVNVVGRSKEEVVEKVERVFTLLKGSREKLPAP

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR)

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786733, Length=363, Percent_Identity=33.6088154269972, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1788155, Length=377, Percent_Identity=25.4641909814324, Blast_Score=83, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324702, Length=381, Percent_Identity=30.1837270341207, Blast_Score=182, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURK_AQUAE (O66608)

Other databases:

- EMBL:   AE000657
- PIR:   D70322
- RefSeq:   NP_213168.1
- PDB:   2Z04
- PDBsum:   2Z04
- ProteinModelPortal:   O66608
- SMR:   O66608
- GeneID:   1192842
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_245
- NMPDR:   fig|224324.1.peg.183
- HOGENOM:   HBG516369
- OMA:   LAVEMFV
- PhylomeDB:   O66608
- ProtClustDB:   PRK06019
- BioCyc:   AAEO224324:AQ_245-MONOMER
- BRENDA:   4.1.1.21
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF1
- TIGRFAMs:   TIGR01161

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.18

Molecular weight: Translated: 41988; Mature: 41988

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 93-93 BINDING 132-132 BINDING 143-143 BINDING 176-176

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVGILGGGQLGWMTILEGRKLGFKFHVLEDKENAPACRVADRCFRTGQISEFVDSCDI
CEEEEEECCCCCCHHEEECCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHCCCE
ITYEFEHIKDEVLEKCESKLIPNPQALYVKKSRIREKLFLKKHGFPVPEFLVIKRDEIID
EEEHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEHHHHHH
ALKSFKLPVVIKAEKLGYDGKGQYRIKKLEDANQVVKNHDKEESFIIEEFVKFEAEISCI
HHHHCCCCEEEEHHHCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEE
GVRDREGKTYFYPQPFNKHEEGILIYNYVPYAKLKEAEEITKRLMELLDIVGVFTVEFFL
EEECCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LKDGRVLINEFAPRVHNTGHWTLDGAYTSQFENLLRAITEMPLGSTELKLPSGMVNILGK
ECCCCHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHCC
SYEEIPLKEILSVEGAKLYWYGKEKKPRRKVGHVNVVGRSKEEVVEKVERVFTLLKGSRE
CCCCCCHHHHHCCCCCEEEEECCCCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCC
KLPAP
CCCCC
>Mature Secondary Structure
MLTVGILGGGQLGWMTILEGRKLGFKFHVLEDKENAPACRVADRCFRTGQISEFVDSCDI
CEEEEEECCCCCCHHEEECCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHCCCE
ITYEFEHIKDEVLEKCESKLIPNPQALYVKKSRIREKLFLKKHGFPVPEFLVIKRDEIID
EEEHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEHHHHHH
ALKSFKLPVVIKAEKLGYDGKGQYRIKKLEDANQVVKNHDKEESFIIEEFVKFEAEISCI
HHHHCCCCEEEEHHHCCCCCCCCEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEE
GVRDREGKTYFYPQPFNKHEEGILIYNYVPYAKLKEAEEITKRLMELLDIVGVFTVEFFL
EEECCCCCEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
LKDGRVLINEFAPRVHNTGHWTLDGAYTSQFENLLRAITEMPLGSTELKLPSGMVNILGK
ECCCCHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHCC
SYEEIPLKEILSVEGAKLYWYGKEKKPRRKVGHVNVVGRSKEEVVEKVERVFTLLKGSRE
CCCCCCHHHHHCCCCCEEEEECCCCCCCHHCCEEEEECCCHHHHHHHHHHHHHHHHCCCC
KLPAP
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320