Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 15605750

GI number: 15605750

Start: 110616

End: 111377

Strand: Direct

Name: hisF

Synonym: aq_181

Alternate gene names: 15605750

Gene position: 110616-111377 (Clockwise)

Preceding gene: 15605741

Following gene: 15605759

Centisome position: 7.13

GC content: 47.9

Gene sequence:

>762_bases
ATGCTGGCAAAGAGAATAATTCCCTGCCTTGACGTGGATAAAGGGAGAGTTGTGAAAGGTGTGAAGTTCCTAAACCTCAG
GGACGCGGGAGACCCAGTGGAAGTGGCAAAAAGGTACGAAGAAGAAGGAGCGGATGAACTGGTATTCCTGGACATAACAG
CCTCGGCGGAAGACAGGGAAATAATGATAGACGTAGTAAAGAAGGTTGCCGAAACCGTCTTCATGCCCTTCACAGTGGGA
GGAGGGATAAGGAGCTTGGAGGATATGAGGAGACTCTTAGAAGCGGGAGCGGACAAGATATCTATAAACACGGCAGCGGT
GAAGAATCCGAATCTTATTTACGAGGGGGCAAAGAGGTTCGGATCTCAGTGTATAGTGGTTGCTATAGACGCAAAAAGGA
AGGGAAATTCGTGGGAGGTTTACATACACGGTGGAAGAACACCTACGGGACTTGACGCTGTTGAGTGGGCTAAAAAAGTT
GAAAGTTTGGGAGCTGGAGAGATTCTCTTGACTTCCATGGACAGAGACGGGACAAAGGACGGGTACGATATAGAACTAAA
CAGAGCCATCTCGGAAGCGGTGAACATACCCGTTATAGCTTCTGGGGGTGCGGGCAAGAAGGAACACTTTTACGAAGTTT
TTTCAAAGACAAAGGTAGAGGCTGCCCTTGCCGCTTCAGTATTCCACTTCAGGGAAATAAGTATTCCGGAGCTGAAGGAG
TATCTTCTGGAAAGAGGTATTAACGTGAGACCTCTTGACTGA

Upstream 100 bases:

>100_bases
TATCGCAAGTGCCACCGCTAAGAGTGCGTAAACTACGTGCGAGTACTCCATTTCCCTTACCTCTTTTGCAATTGGCTCAG
AAAATTATAACATAAATTTG

Downstream 100 bases:

>100_bases
CGCATTTTAAAGCTTCCTTTCCCAATACTTCTTCCAAAACAAACAGAGCTCTTTTTTTTGCAATTTCTGCATTTCTTTCT
GGATTTTTAGAGTTTGCATC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLAKRIIPCLDVDKGRVVKGVKFLNLRDAGDPVEVAKRYEEEGADELVFLDITASAEDREIMIDVVKKVAETVFMPFTVG
GGIRSLEDMRRLLEAGADKISINTAAVKNPNLIYEGAKRFGSQCIVVAIDAKRKGNSWEVYIHGGRTPTGLDAVEWAKKV
ESLGAGEILLTSMDRDGTKDGYDIELNRAISEAVNIPVIASGGAGKKEHFYEVFSKTKVEAALAASVFHFREISIPELKE
YLLERGINVRPLD

Sequences:

>Translated_253_residues
MLAKRIIPCLDVDKGRVVKGVKFLNLRDAGDPVEVAKRYEEEGADELVFLDITASAEDREIMIDVVKKVAETVFMPFTVG
GGIRSLEDMRRLLEAGADKISINTAAVKNPNLIYEGAKRFGSQCIVVAIDAKRKGNSWEVYIHGGRTPTGLDAVEWAKKV
ESLGAGEILLTSMDRDGTKDGYDIELNRAISEAVNIPVIASGGAGKKEHFYEVFSKTKVEAALAASVFHFREISIPELKE
YLLERGINVRPLD
>Mature_253_residues
MLAKRIIPCLDVDKGRVVKGVKFLNLRDAGDPVEVAKRYEEEGADELVFLDITASAEDREIMIDVVKKVAETVFMPFTVG
GGIRSLEDMRRLLEAGADKISINTAAVKNPNLIYEGAKRFGSQCIVVAIDAKRKGNSWEVYIHGGRTPTGLDAVEWAKKV
ESLGAGEILLTSMDRDGTKDGYDIELNRAISEAVNIPVIASGGAGKKEHFYEVFSKTKVEAALAASVFHFREISIPELKE
YLLERGINVRPLD

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=50.78125, Blast_Score=245, Evalue=2e-66,
Organism=Escherichia coli, GI87082028, Length=206, Percent_Identity=30.5825242718447, Blast_Score=92, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=38.2165605095541, Blast_Score=184, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_AQUAE (O66567)

Other databases:

- EMBL:   AE000657
- PIR:   C70317
- RefSeq:   NP_213127.1
- ProteinModelPortal:   O66567
- SMR:   O66567
- GeneID:   1192709
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_181
- NMPDR:   fig|224324.1.peg.142
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   O66567
- ProtClustDB:   PRK02083
- BioCyc:   AAEO224324:AQ_181-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27796; Mature: 27796

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVDKGRVVKGVKFLNLRDAGDPVEVAKRYEEEGADELVFLDITASAEDRE
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHH
IMIDVVKKVAETVFMPFTVGGGIRSLEDMRRLLEAGADKISINTAAVKNPNLIYEGAKRF
HHHHHHHHHHHHEECCEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
GSQCIVVAIDAKRKGNSWEVYIHGGRTPTGLDAVEWAKKVESLGAGEILLTSMDRDGTKD
CCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDIELNRAISEAVNIPVIASGGAGKKEHFYEVFSKTKVEAALAASVFHFREISIPELKE
CCEEEHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YLLERGINVRPLD
HHHHCCCCEECCC
>Mature Secondary Structure
MLAKRIIPCLDVDKGRVVKGVKFLNLRDAGDPVEVAKRYEEEGADELVFLDITASAEDRE
CCHHHCCCCCCCCCCCEEECEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHH
IMIDVVKKVAETVFMPFTVGGGIRSLEDMRRLLEAGADKISINTAAVKNPNLIYEGAKRF
HHHHHHHHHHHHEECCEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHH
GSQCIVVAIDAKRKGNSWEVYIHGGRTPTGLDAVEWAKKVESLGAGEILLTSMDRDGTKD
CCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYDIELNRAISEAVNIPVIASGGAGKKEHFYEVFSKTKVEAALAASVFHFREISIPELKE
CCEEEHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
YLLERGINVRPLD
HHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320