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The map label for this gene is amtB

Identifier: 15605698

GI number: 15605698

Start: 69169

End: 70440

Strand: Direct

Name: amtB

Synonym: aq_112

Alternate gene names: 15605698

Gene position: 69169-70440 (Clockwise)

Preceding gene: 15605697

Following gene: 15605699

Centisome position: 4.46

GC content: 46.62

Gene sequence:

>1272_bases
ATGAGAGCGCTAGGATTTATAGGGATTATTCTATCAATTTTTTCCTCTTTTGCGTATGCATCTGAAGCCAAGTTAGACAC
TGGTAACACAGCTTGGATGCTCGTAGCTTCAGCCCTTGTGGTGTTTATGACAGTTCCCGGGCTCGCTCTTTTCTATGGAG
GTTTAGACAAATCAAAGAGCATCCTAAACACGATAGCGATGTCCTTTTCAGCCTTTGCGGTTGTAACACTAACGTGGATA
TTCGTAGGTTACTCAGTAGCCTACGGAGACGATATTTTTGGTTTTATAGGAAATCCATTCCAGTACGTATTAGGTAAGGG
TATAAGCGGGATAAATTCCGATACCGGATACCCTGCACTTCTGGATCTGATGTTCCAGCTCACTTTCGCAACAATAACTA
CCGCTTTAATTTCGGGTTCCTTCGTAGGAAGGATGAAGTTTTCAGCCTGGATATTGTTTGCAATACTCTGGAGCGTATTT
GTTTATCCGCCTGTAGCCCACTGGGTATGGGGAGGCGGATTTCTGGCTAACGACGGAGCCCTTGACTTTGCAGGAGGAAC
GGTAGTCCACATTAACGCGGGTATAGCGGGTTTAGTAGGTGCGTTGATCCTCGGAAGAAGGAAAGACACATCTCTTATTC
CCAACAACGTTCCACTGGTAGCCCTCGGTGCGGGAATACTGTGGTTCGGGTGGTTTGGATTCAACGCCGGTTCTGCTCTC
GGAGCTAATGAGAGCGCAGCTTGGGCAATGATAAATACCACGGTTGCAACTTCAACGGCAGCACTCGCATGGATGTTTAC
CGAATGGCTTCACGTCGGTAAACCTACAGTTGTGGGAATATCTTCAGGTATCGTTGCAGGACTTGTCGCAATAACTCCCG
CGGCAGGTTTTGTAAACCTTATCGGTTCAATTTTTATAGGAGCTATAGCATCCGTGTGTGCTTACTTTATGGTTGCTCTT
GTAAAACCAAAATTCGGATACGACGATGCACTTGACGTGTTCGGAATTCACGGAGTACGCGGAATAGTGGGAGCAGTTTT
AACGGGAGTATTTGCGGATCCGAACGTGGGAGGAACTCCTGGACTGCTCTACGGCAATCCAAAACAGGTTCTTATACAAA
TAGAAGGCGTTATCGCTACTATACTCTACTCCGCTATCCTCACAGCCGTTATACTGCTGGTTCTGAAAGCAGTAGTAGGT
CTAAGAGTTTCCGAAGAAGAGGAGCTCGAACTGGACAGCTCCCTACACGGTGAAAAGGCTTACAACCTATAA

Upstream 100 bases:

>100_bases
CCCCACCCCAAAGAATTTGAACTTTACTGGGACATTTAAACAAATTCTCGGGGGCGTTTCGCCCCTTTTATAAAAACTTT
TTTAAAAAAGGAGGGCAAAA

Downstream 100 bases:

>100_bases
AGGAGGTTTAGTTATGAAAAAGTACGTTCTTCCTCTCTTGTTTTTAAGCTCTCTTTCTCTCGCTGAGGAAAAAAGTCTTT
ACCTGAAGGTATCCCAACCG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MRALGFIGIILSIFSSFAYASEAKLDTGNTAWMLVASALVVFMTVPGLALFYGGLDKSKSILNTIAMSFSAFAVVTLTWI
FVGYSVAYGDDIFGFIGNPFQYVLGKGISGINSDTGYPALLDLMFQLTFATITTALISGSFVGRMKFSAWILFAILWSVF
VYPPVAHWVWGGGFLANDGALDFAGGTVVHINAGIAGLVGALILGRRKDTSLIPNNVPLVALGAGILWFGWFGFNAGSAL
GANESAAWAMINTTVATSTAALAWMFTEWLHVGKPTVVGISSGIVAGLVAITPAAGFVNLIGSIFIGAIASVCAYFMVAL
VKPKFGYDDALDVFGIHGVRGIVGAVLTGVFADPNVGGTPGLLYGNPKQVLIQIEGVIATILYSAILTAVILLVLKAVVG
LRVSEEEELELDSSLHGEKAYNL

Sequences:

>Translated_423_residues
MRALGFIGIILSIFSSFAYASEAKLDTGNTAWMLVASALVVFMTVPGLALFYGGLDKSKSILNTIAMSFSAFAVVTLTWI
FVGYSVAYGDDIFGFIGNPFQYVLGKGISGINSDTGYPALLDLMFQLTFATITTALISGSFVGRMKFSAWILFAILWSVF
VYPPVAHWVWGGGFLANDGALDFAGGTVVHINAGIAGLVGALILGRRKDTSLIPNNVPLVALGAGILWFGWFGFNAGSAL
GANESAAWAMINTTVATSTAALAWMFTEWLHVGKPTVVGISSGIVAGLVAITPAAGFVNLIGSIFIGAIASVCAYFMVAL
VKPKFGYDDALDVFGIHGVRGIVGAVLTGVFADPNVGGTPGLLYGNPKQVLIQIEGVIATILYSAILTAVILLVLKAVVG
LRVSEEEELELDSSLHGEKAYNL
>Mature_423_residues
MRALGFIGIILSIFSSFAYASEAKLDTGNTAWMLVASALVVFMTVPGLALFYGGLDKSKSILNTIAMSFSAFAVVTLTWI
FVGYSVAYGDDIFGFIGNPFQYVLGKGISGINSDTGYPALLDLMFQLTFATITTALISGSFVGRMKFSAWILFAILWSVF
VYPPVAHWVWGGGFLANDGALDFAGGTVVHINAGIAGLVGALILGRRKDTSLIPNNVPLVALGAGILWFGWFGFNAGSAL
GANESAAWAMINTTVATSTAALAWMFTEWLHVGKPTVVGISSGIVAGLVAITPAAGFVNLIGSIFIGAIASVCAYFMVAL
VKPKFGYDDALDVFGIHGVRGIVGAVLTGVFADPNVGGTPGLLYGNPKQVLIQIEGVIATILYSAILTAVILLVLKAVVG
LRVSEEEELELDSSLHGEKAYNL

Specific function: Involved in the uptake of ammonia

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family

Homologues:

Organism=Escherichia coli, GI1786656, Length=419, Percent_Identity=44.3914081145585, Blast_Score=334, Evalue=7e-93,
Organism=Caenorhabditis elegans, GI17550180, Length=448, Percent_Identity=26.3392857142857, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=25.8503401360544, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32564042, Length=427, Percent_Identity=27.4004683840749, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI133931100, Length=439, Percent_Identity=27.3348519362187, Blast_Score=95, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6325396, Length=417, Percent_Identity=38.3693045563549, Blast_Score=241, Evalue=2e-64,
Organism=Saccharomyces cerevisiae, GI6324187, Length=429, Percent_Identity=38.6946386946387, Blast_Score=236, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6321559, Length=417, Percent_Identity=36.21103117506, Blast_Score=219, Evalue=9e-58,
Organism=Drosophila melanogaster, GI161078314, Length=408, Percent_Identity=28.4313725490196, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMT_AQUAE (O66515)

Other databases:

- EMBL:   AE000657
- PIR:   H70310
- RefSeq:   NP_213075.1
- ProteinModelPortal:   O66515
- SMR:   O66515
- GeneID:   1192526
- GenomeReviews:   AE000657_GR
- KEGG:   aae:aq_112
- NMPDR:   fig|224324.1.peg.90
- HOGENOM:   HBG677626
- OMA:   GNNSWIG
- PhylomeDB:   O66515
- BioCyc:   AAEO224324:AQ_112-MONOMER
- InterPro:   IPR001905
- InterPro:   IPR018047
- PANTHER:   PTHR11730
- TIGRFAMs:   TIGR00836

Pfam domain/function: PF00909 Ammonium_transp; SSF111352 RH_like_transpt

EC number: NA

Molecular weight: Translated: 44323; Mature: 44323

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xbfd87c8)-; HASH(0xaff7880)-; HASH(0xc13332c)-; HASH(0xc12f188)-; HASH(0xbd84fec)-; HASH(0xaf23f30)-; HASH(0xbb301b0)-; HASH(0x40678894)-; HASH(0xc0bd7b8)-; HASH(0xbfc7f64)-; HASH(0xc13ba88)-;

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALGFIGIILSIFSSFAYASEAKLDTGNTAWMLVASALVVFMTVPGLALFYGGLDKSKS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ILNTIAMSFSAFAVVTLTWIFVGYSVAYGDDIFGFIGNPFQYVLGKGISGINSDTGYPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCHHH
LDLMFQLTFATITTALISGSFVGRMKFSAWILFAILWSVFVYPPVAHWVWGGGFLANDGA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEECCCC
LDFAGGTVVHINAGIAGLVGALILGRRKDTSLIPNNVPLVALGAGILWFGWFGFNAGSAL
EEECCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCC
GANESAAWAMINTTVATSTAALAWMFTEWLHVGKPTVVGISSGIVAGLVAITPAAGFVNL
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCHHHHHH
IGSIFIGAIASVCAYFMVALVKPKFGYDDALDVFGIHGVRGIVGAVLTGVFADPNVGGTP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLLYGNPKQVLIQIEGVIATILYSAILTAVILLVLKAVVGLRVSEEEELELDSSLHGEKA
CEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCC
YNL
CCH
>Mature Secondary Structure
MRALGFIGIILSIFSSFAYASEAKLDTGNTAWMLVASALVVFMTVPGLALFYGGLDKSKS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
ILNTIAMSFSAFAVVTLTWIFVGYSVAYGDDIFGFIGNPFQYVLGKGISGINSDTGYPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHCCCCCCCCCCCCCHHH
LDLMFQLTFATITTALISGSFVGRMKFSAWILFAILWSVFVYPPVAHWVWGGGFLANDGA
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEECCCC
LDFAGGTVVHINAGIAGLVGALILGRRKDTSLIPNNVPLVALGAGILWFGWFGFNAGSAL
EEECCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCC
GANESAAWAMINTTVATSTAALAWMFTEWLHVGKPTVVGISSGIVAGLVAITPAAGFVNL
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCHHHHHH
IGSIFIGAIASVCAYFMVALVKPKFGYDDALDVFGIHGVRGIVGAVLTGVFADPNVGGTP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GLLYGNPKQVLIQIEGVIATILYSAILTAVILLVLKAVVGLRVSEEEELELDSSLHGEKA
CEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCC
YNL
CCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320