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The map label for this gene is scrB [H]

Identifier: 15603713

GI number: 15603713

Start: 2088316

End: 2089815

Strand: Reverse

Name: scrB [H]

Synonym: PM1848

Alternate gene names: 15603713

Gene position: 2089815-2088316 (Counterclockwise)

Preceding gene: 15603714

Following gene: 15603712

Centisome position: 92.57

GC content: 40.93

Gene sequence:

>1500_bases
ATGCCTTTCTCGCTGAGTAAAGTGCGGTCATTTTTCTGCCATTTTTGGACATCACCTATGTATATTTTTAATCAAGGCAA
ATACAAAAGTTTGCACGCACAGCAAGTCGGGGAACTTGCACAGATTCGACAACATGTACTGCGCGATACACAATTTCGCC
CGACTTATCATCTTGCGCCAGAAACAGGCTTATTAAATGATCCTAATGGTTTAGTGTTTGATGGCGAAAAATATCATATT
TTTTATCAATGGTTCCCGTACGCCGCAATACACGGAATGAAACACTGGAAACATTTTATGACGTATGATTTTCACCATTT
TCAAGTTGCAGATCCATTGATTCCAGATGAACTATTTGAATCACACGGTTGCTATTCGGGTGGCGCACTGTTATGGCAAG
ATCAGATTGTTGCTTTTTATACTGGCAACACGCGCAACGCAGAAAATCAACGTATTCCACACCAAAACATCGCGATTTTT
AAGAAAGACGGCACACTCTTAAAAAAACGTTGCTTGTTAAATCAAGCCCCAACAGGTTATACCGAGCACGTCAGAGATCC
CAAACCATTTATAACGGAAGAAGGCAAAATTCGCTTTATTCTCGGTGCACAACGTGAAGATCTTTCTGGCACAGCGTTAA
TCTATGAAATGACCGATCTTGATGCCACACCACAACTTATCGGTGAATTCAAACTCCCTGATTTTGATAACCAGCAGGTA
TTTATGTGGGAATGCCCTGATTTGCTCAAGCTTCAAGAACAAGATCTGTTTCTTTGGTCCCCTCAAGGCAAAGCACAAAA
AACCTACTGTTTCCAAAATCACTATCATGCCACTTATGCGCTGGGTCAATATGCCAATTTGCACTTTTCCGCACAACATA
TCGAAGAACTGGATTATGGCTTTGACTTTTATGCGCCACAAAGTATTCAGGGATCGGAAGGTATCTATTATGCTTGGGTT
GGCTTACCAGACTTAACCTATCCAACGGATGAATACCAATGGCATTCTATGCTAAGTTTGCCACGTCAATTTTCAATTCA
GCAGGGAATACTTTATCAGCGTCCAATTCCACAAGTGTATGCCCAGTGTCTGCCTGCGCATAAATATTGGGTGGAGGGCA
AGCAACAAATTCCACATTTAGATCGTAGCTATGTTGCTTTTGATGCTGAAAATCAGCCTTTCACACTGCGTTTTTTCACA
AACCAGCGAGGACAACAACTGATTTTACGCTATCAAAATGGCATACTGACGCTAGATAGAACATACACAACACAAACCGC
CTTGATGCAACAATTTGGTGAACAACGCCATTGTATTTTGCCTCAATTAAACCATGTGGAAATATTCTTTGATCGTTCTG
TCGTGGAAATTTTTTTAAATCAAGGTGAGAAAGTGATGACTTCACGTTTCTTTATTGCGGATCGAGAAAATGCGTTAGAT
AGCTCTCGCCCGCTCTGTGTTTCTGTGGCAGAATTAAAACCCATTATCTTAACACCTTAA

Upstream 100 bases:

>100_bases
ACCGCACTTGTCGAGATCATCAAACAAGCCAATGCATGCGGCGCATTAGCAACAACGGCAAAGGGCGCCATGTCTGCGTT
ACCCAATAAACAGCAATTAG

Downstream 100 bases:

>100_bases
GTTTTCATTTATTTCTCTTTACAGCATGAGCATTACCAATAAAAAACGTATCACCCTCAGCGATATTGCGAAATGTTGTG
GTTTATCCACCACTACCGTC

Product: ScrB

Products: NA

Alternate protein names: Sucrase; Invertase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MPFSLSKVRSFFCHFWTSPMYIFNQGKYKSLHAQQVGELAQIRQHVLRDTQFRPTYHLAPETGLLNDPNGLVFDGEKYHI
FYQWFPYAAIHGMKHWKHFMTYDFHHFQVADPLIPDELFESHGCYSGGALLWQDQIVAFYTGNTRNAENQRIPHQNIAIF
KKDGTLLKKRCLLNQAPTGYTEHVRDPKPFITEEGKIRFILGAQREDLSGTALIYEMTDLDATPQLIGEFKLPDFDNQQV
FMWECPDLLKLQEQDLFLWSPQGKAQKTYCFQNHYHATYALGQYANLHFSAQHIEELDYGFDFYAPQSIQGSEGIYYAWV
GLPDLTYPTDEYQWHSMLSLPRQFSIQQGILYQRPIPQVYAQCLPAHKYWVEGKQQIPHLDRSYVAFDAENQPFTLRFFT
NQRGQQLILRYQNGILTLDRTYTTQTALMQQFGEQRHCILPQLNHVEIFFDRSVVEIFLNQGEKVMTSRFFIADRENALD
SSRPLCVSVAELKPIILTP

Sequences:

>Translated_499_residues
MPFSLSKVRSFFCHFWTSPMYIFNQGKYKSLHAQQVGELAQIRQHVLRDTQFRPTYHLAPETGLLNDPNGLVFDGEKYHI
FYQWFPYAAIHGMKHWKHFMTYDFHHFQVADPLIPDELFESHGCYSGGALLWQDQIVAFYTGNTRNAENQRIPHQNIAIF
KKDGTLLKKRCLLNQAPTGYTEHVRDPKPFITEEGKIRFILGAQREDLSGTALIYEMTDLDATPQLIGEFKLPDFDNQQV
FMWECPDLLKLQEQDLFLWSPQGKAQKTYCFQNHYHATYALGQYANLHFSAQHIEELDYGFDFYAPQSIQGSEGIYYAWV
GLPDLTYPTDEYQWHSMLSLPRQFSIQQGILYQRPIPQVYAQCLPAHKYWVEGKQQIPHLDRSYVAFDAENQPFTLRFFT
NQRGQQLILRYQNGILTLDRTYTTQTALMQQFGEQRHCILPQLNHVEIFFDRSVVEIFLNQGEKVMTSRFFIADRENALD
SSRPLCVSVAELKPIILTP
>Mature_498_residues
PFSLSKVRSFFCHFWTSPMYIFNQGKYKSLHAQQVGELAQIRQHVLRDTQFRPTYHLAPETGLLNDPNGLVFDGEKYHIF
YQWFPYAAIHGMKHWKHFMTYDFHHFQVADPLIPDELFESHGCYSGGALLWQDQIVAFYTGNTRNAENQRIPHQNIAIFK
KDGTLLKKRCLLNQAPTGYTEHVRDPKPFITEEGKIRFILGAQREDLSGTALIYEMTDLDATPQLIGEFKLPDFDNQQVF
MWECPDLLKLQEQDLFLWSPQGKAQKTYCFQNHYHATYALGQYANLHFSAQHIEELDYGFDFYAPQSIQGSEGIYYAWVG
LPDLTYPTDEYQWHSMLSLPRQFSIQQGILYQRPIPQVYAQCLPAHKYWVEGKQQIPHLDRSYVAFDAENQPFTLRFFTN
QRGQQLILRYQNGILTLDRTYTTQTALMQQFGEQRHCILPQLNHVEIFFDRSVVEIFLNQGEKVMTSRFFIADRENALDS
SRPLCVSVAELKPIILTP

Specific function: Enables the bacterium to metabolize sucrose as a sole carbon source [H]

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=346, Percent_Identity=25.7225433526012, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232 [H]

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N [H]

EC number: =3.2.1.26 [H]

Molecular weight: Translated: 58362; Mature: 58231

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSLSKVRSFFCHFWTSPMYIFNQGKYKSLHAQQVGELAQIRQHVLRDTQFRPTYHLAP
CCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
ETGLLNDPNGLVFDGEKYHIFYQWFPYAAIHGMKHWKHFMTYDFHHFQVADPLIPDELFE
CCCCCCCCCCEEECCCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
SHGCYSGGALLWQDQIVAFYTGNTRNAENQRIPHQNIAIFKKDGTLLKKRCLLNQAPTGY
HCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCC
TEHVRDPKPFITEEGKIRFILGAQREDLSGTALIYEMTDLDATPQLIGEFKLPDFDNQQV
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCEE
FMWECPDLLKLQEQDLFLWSPQGKAQKTYCFQNHYHATYALGQYANLHFSAQHIEELDYG
EEECCCHHHHCCCCCEEEECCCCCCCCEEEECCCCEEEEEECCCCCEEECHHHHHHHHCC
FDFYAPQSIQGSEGIYYAWVGLPDLTYPTDEYQWHSMLSLPRQFSIQQGILYQRPIPQVY
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHCCCEECCCCHHHH
AQCLPAHKYWVEGKQQIPHLDRSYVAFDAENQPFTLRFFTNQRGQQLILRYQNGILTLDR
HHHCCCHHHHCCCHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCEEEEEC
TYTTQTALMQQFGEQRHCILPQLNHVEIFFDRSVVEIFLNQGEKVMTSRFFIADRENALD
CHHHHHHHHHHHCCCCCEECCCCCCEEEEECCHHHHHHHCCCCEEEEEEEEEECCCCCCC
SSRPLCVSVAELKPIILTP
CCCCEEEEHHHCCCEEECC
>Mature Secondary Structure 
PFSLSKVRSFFCHFWTSPMYIFNQGKYKSLHAQQVGELAQIRQHVLRDTQFRPTYHLAP
CCCHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
ETGLLNDPNGLVFDGEKYHIFYQWFPYAAIHGMKHWKHFMTYDFHHFQVADPLIPDELFE
CCCCCCCCCCEEECCCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHH
SHGCYSGGALLWQDQIVAFYTGNTRNAENQRIPHQNIAIFKKDGTLLKKRCLLNQAPTGY
HCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCC
TEHVRDPKPFITEEGKIRFILGAQREDLSGTALIYEMTDLDATPQLIGEFKLPDFDNQQV
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCCEE
FMWECPDLLKLQEQDLFLWSPQGKAQKTYCFQNHYHATYALGQYANLHFSAQHIEELDYG
EEECCCHHHHCCCCCEEEECCCCCCCCEEEECCCCEEEEEECCCCCEEECHHHHHHHHCC
FDFYAPQSIQGSEGIYYAWVGLPDLTYPTDEYQWHSMLSLPRQFSIQQGILYQRPIPQVY
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCCHHHHHHCCCEECCCCHHHH
AQCLPAHKYWVEGKQQIPHLDRSYVAFDAENQPFTLRFFTNQRGQQLILRYQNGILTLDR
HHHCCCHHHHCCCHHHCCCCCCCEEEEECCCCCEEEEEECCCCCCEEEEEECCCEEEEEC
TYTTQTALMQQFGEQRHCILPQLNHVEIFFDRSVVEIFLNQGEKVMTSRFFIADRENALD
CHHHHHHHHHHHCCCCCEECCCCCCEEEEECCHHHHHHHCCCCEEEEEEEEEECCCCCCC
SSRPLCVSVAELKPIILTP
CCCCEEEEHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2551785 [H]