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The map label for this gene is hemX [C]

Identifier: 15603679

GI number: 15603679

Start: 2043672

End: 2045075

Strand: Direct

Name: hemX [C]

Synonym: PM1814

Alternate gene names: 15603679

Gene position: 2043672-2045075 (Clockwise)

Preceding gene: 15603678

Following gene: 15603680

Centisome position: 90.53

GC content: 40.24

Gene sequence:

>1404_bases
ATGGCTGAAAATAAAATAACCTCTTCTGAAAGCAGTACTGTACCAAAAAATGAGGGAAGTACAGTCGATAAGAAAAAGAC
CAGTGGAAAAGTCACGGTAGATCCAAAAGAACAGGCGGTTGCACAAGATGCGCCTGCTACGCAGACAGAAACCGCAAAAA
ACGAGGTGCCAGAGAAAAAGGATGCAGGCAAAATGCGAGATGAGATGAAAGAAAGTGTCAAAAAAGAACCTGCTAAGCAA
GCGCGTGCTGAATCAAAAAACACCGCCGAAACAGTTGTGGTGAAGAAAGGTGGTGGCACTGGTTTAGCGTTACTAGCGCT
GTTAGTTGCGCTTGGTCTTGGTGCGGCAGGTTATTACTTTGGTTTACAACAAGTGGATCATATTCAACATAAACTCAGCG
CCCTTGAACAGCAAACAACCAATATCACACCCGCAAAAGTGGACCTTCCGACGTTTGAACAAGAGCGTCAGCAAATTGCC
CAATTAATGCGTTCCATACGTGATAATGAAGAAAAATTACAACAGTTAGAAAAAGAGCTTGCAGGCAAAGACCATTCTCT
TTCTGCATTGCAAAATCAAGTAAACCGCATGAGTGCTGCCGCGAAACAACAACAGCCAAATGATTGGTTATTATCAGAAG
CAGATTTTTTACTAAATAATGCATTACGCAAGTTAGTTTTAGATAACGATGTGGATACAGGTGTTTCCTTACTCAAGATT
GCGGATGAAACTTTGGAAAAAATGTCAGATCCGCGTGTTAGCATGGTTAGGACCGCGATCAATAATGATTTAAAACAATT
ACTTGCAGTGAATAATGTCGATCAGAATGTGATCATGCAACGTTTATCACAGCTGGCTAATACCATTGATGAGTTGACTG
TATTAGACGTGAATTTTGATGCAGTGAACAATAATGAAAAACTTACTGATTCGTTAGATGATTGGAAAGAAAACGCCGAA
AAAAGCGCGACTTCTTTCTTAAATCACTTTATTCGCATTACACCAAGAAATACGAGTGATAAGGTGCTACTAGCCCCGAA
TCAGGATATTTATTTACGTGAAAATATCCGATTACGTTTGCAAATTGCCATTATGGCGGTACCACGTCAACAAGATGAAT
TGTATAAACAATCATTAGAAACTGTAGCATCTTGGATCAGAAGTTATTTTGATACTAACTCTGAAGTGGCGCAGAATTTC
TTGAAAACTCTAGATGAATTAGCGGAGCAATCTATCTATGTGGATGTACCTTCTCAATTACAGAGTTTAAATGCGTTGGA
TAAACTACTGGATCGCCCTTCACAAGATGTTAAAAAGATCGAACTTTCCGTGGATAAGGCGTTAGTTGAGGAAACAACAG
AGAGTGCTCCTTCGAGTGACGCTTCGACGGCAGAACAACAGTAG

Upstream 100 bases:

>100_bases
GTGGTTTCACCGCGAGCAGATAACGTGCATTTACTAAAAACATTGCTGTCTGCGGACTAACTAGTGTTGAATTCTAATGA
TCATAAAGGAAATGGGTATT

Downstream 100 bases:

>100_bases
AGGAAATAGATTATGTTTAGAGTGCTATTTTTAATGCTGGTGTTACTAGCAGGCTTAATTGCAGGACCGTATTTATCGGG
TAAACAAGGCTATGTCTTGA

Product: hypothetical protein

Products: S-adenosyl-L-homocysteine; precorrin-1; Precorrin 2

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MAENKITSSESSTVPKNEGSTVDKKKTSGKVTVDPKEQAVAQDAPATQTETAKNEVPEKKDAGKMRDEMKESVKKEPAKQ
ARAESKNTAETVVVKKGGGTGLALLALLVALGLGAAGYYFGLQQVDHIQHKLSALEQQTTNITPAKVDLPTFEQERQQIA
QLMRSIRDNEEKLQQLEKELAGKDHSLSALQNQVNRMSAAAKQQQPNDWLLSEADFLLNNALRKLVLDNDVDTGVSLLKI
ADETLEKMSDPRVSMVRTAINNDLKQLLAVNNVDQNVIMQRLSQLANTIDELTVLDVNFDAVNNNEKLTDSLDDWKENAE
KSATSFLNHFIRITPRNTSDKVLLAPNQDIYLRENIRLRLQIAIMAVPRQQDELYKQSLETVASWIRSYFDTNSEVAQNF
LKTLDELAEQSIYVDVPSQLQSLNALDKLLDRPSQDVKKIELSVDKALVEETTESAPSSDASTAEQQ

Sequences:

>Translated_467_residues
MAENKITSSESSTVPKNEGSTVDKKKTSGKVTVDPKEQAVAQDAPATQTETAKNEVPEKKDAGKMRDEMKESVKKEPAKQ
ARAESKNTAETVVVKKGGGTGLALLALLVALGLGAAGYYFGLQQVDHIQHKLSALEQQTTNITPAKVDLPTFEQERQQIA
QLMRSIRDNEEKLQQLEKELAGKDHSLSALQNQVNRMSAAAKQQQPNDWLLSEADFLLNNALRKLVLDNDVDTGVSLLKI
ADETLEKMSDPRVSMVRTAINNDLKQLLAVNNVDQNVIMQRLSQLANTIDELTVLDVNFDAVNNNEKLTDSLDDWKENAE
KSATSFLNHFIRITPRNTSDKVLLAPNQDIYLRENIRLRLQIAIMAVPRQQDELYKQSLETVASWIRSYFDTNSEVAQNF
LKTLDELAEQSIYVDVPSQLQSLNALDKLLDRPSQDVKKIELSVDKALVEETTESAPSSDASTAEQQ
>Mature_466_residues
AENKITSSESSTVPKNEGSTVDKKKTSGKVTVDPKEQAVAQDAPATQTETAKNEVPEKKDAGKMRDEMKESVKKEPAKQA
RAESKNTAETVVVKKGGGTGLALLALLVALGLGAAGYYFGLQQVDHIQHKLSALEQQTTNITPAKVDLPTFEQERQQIAQ
LMRSIRDNEEKLQQLEKELAGKDHSLSALQNQVNRMSAAAKQQQPNDWLLSEADFLLNNALRKLVLDNDVDTGVSLLKIA
DETLEKMSDPRVSMVRTAINNDLKQLLAVNNVDQNVIMQRLSQLANTIDELTVLDVNFDAVNNNEKLTDSLDDWKENAEK
SATSFLNHFIRITPRNTSDKVLLAPNQDIYLRENIRLRLQIAIMAVPRQQDELYKQSLETVASWIRSYFDTNSEVAQNFL
KTLDELAEQSIYVDVPSQLQSLNALDKLLDRPSQDVKKIELSVDKALVEETTESAPSSDASTAEQQ

Specific function: Siroheme and cobalamin biosynthesis. [C]

COG id: COG2959

COG function: function code H; Uncharacterized enzyme of heme biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli hemX C-terminal region [H]

Homologues:

Organism=Escherichia coli, GI1790235, Length=320, Percent_Identity=39.375, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007470 [H]

Pfam domain/function: PF04375 HemX [H]

EC number: 2.1.1.107

Molecular weight: Translated: 51911; Mature: 51780

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENKITSSESSTVPKNEGSTVDKKKTSGKVTVDPKEQAVAQDAPATQTETAKNEVPEKK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHCCCCCCCHHHHHHCCCCCH
DAGKMRDEMKESVKKEPAKQARAESKNTAETVVVKKGGGTGLALLALLVALGLGAAGYYF
HHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHH
GLQQVDHIQHKLSALEQQTTNITPAKVDLPTFEQERQQIAQLMRSIRDNEEKLQQLEKEL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AGKDHSLSALQNQVNRMSAAAKQQQPNDWLLSEADFLLNNALRKLVLDNDVDTGVSLLKI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ADETLEKMSDPRVSMVRTAINNDLKQLLAVNNVDQNVIMQRLSQLANTIDELTVLDVNFD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECC
AVNNNEKLTDSLDDWKENAEKSATSFLNHFIRITPRNTSDKVLLAPNQDIYLRENIRLRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCCEEEECCCEEEE
QIAIMAVPRQQDELYKQSLETVASWIRSYFDTNSEVAQNFLKTLDELAEQSIYVDVPSQL
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECHHHH
QSLNALDKLLDRPSQDVKKIELSVDKALVEETTESAPSSDASTAEQQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AENKITSSESSTVPKNEGSTVDKKKTSGKVTVDPKEQAVAQDAPATQTETAKNEVPEKK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHCCCCCCCHHHHHHCCCCCH
DAGKMRDEMKESVKKEPAKQARAESKNTAETVVVKKGGGTGLALLALLVALGLGAAGYYF
HHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHH
GLQQVDHIQHKLSALEQQTTNITPAKVDLPTFEQERQQIAQLMRSIRDNEEKLQQLEKEL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AGKDHSLSALQNQVNRMSAAAKQQQPNDWLLSEADFLLNNALRKLVLDNDVDTGVSLLKI
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ADETLEKMSDPRVSMVRTAINNDLKQLLAVNNVDQNVIMQRLSQLANTIDELTVLDVNFD
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECC
AVNNNEKLTDSLDDWKENAEKSATSFLNHFIRITPRNTSDKVLLAPNQDIYLRENIRLRL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEECCCCCEEEECCCEEEE
QIAIMAVPRQQDELYKQSLETVASWIRSYFDTNSEVAQNFLKTLDELAEQSIYVDVPSQL
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEECHHHH
QSLNALDKLLDRPSQDVKKIELSVDKALVEETTESAPSSDASTAEQQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; uroporphyrin III; precorrin-1

Specific reaction: (1) S-adenosyl-L-methionine + uroporphyrin III = S-adenosyl-L-homocysteine + precorrin-1; (2) S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]