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The map label for this gene is nanK

Identifier: 15603577

GI number: 15603577

Start: 1920664

End: 1921557

Strand: Direct

Name: nanK

Synonym: PM1712

Alternate gene names: 15603577

Gene position: 1920664-1921557 (Clockwise)

Preceding gene: 15603576

Following gene: 15603578

Centisome position: 85.08

GC content: 45.86

Gene sequence:

>894_bases
ATGCGCTGTTTAGCATTAGATATTGGTGGAACAAAAATTGCCTCGGCTATTGTAACAGACGGCAAAATAGAACAACGCCA
ACAGATTGCAACGCCACAAGCTGATGCTGCCAATGCGATGCATGACACACTGGCCAACATTCTTGCTTTGTATGCCGGAC
AATTCGATTACGTTGCAGTGGCCTCAACCGGCATTATTAATCATGGTGTGTTAACCGCCTTAAATCCAAAAAATTTAGGT
GGTTTGGCGGAATTTCCCTTAAAAGAAAGCATTGCGCGCCATACGGATAAACCGATTGGTTTACTAAATGATGTGCAAGC
TGCCGCGTGTGCGGAATATAAAGACGAAGATAAAAATGCGGTACAAAATTTTGTTTTTATCACCGTATCCACTGGGGTGG
GTGGTGGCATTATTTTAGAACGCCGTTTATTGACAGAACCTAACGGCGTTGCCGGGCATATCGGACATACATTAGCAGAC
CCAAATGGTCCGGTTTGTGGTTGTGGGCGTGTAGGTTGTGTTGAAGCCGTCGCGGCTGGACGCGCAATTGAAGCCGTTTC
TAGCCAATGGAATCCACCTTGCACACCAAAACAAGCTTTCGAGTTATTTAGAAAAAATGATGAAAAAGCGACCGCACTTA
TCCAGCGTTCAGCTAGCGCGATAGCCAACCTGATTGCGGATTTAGTGATTGGTTTAGATGTACAAAAAGTGGTAGTTGGC
GGTAGTGTTGGTTTAGCGGAAGGTTACCTCCCCTTAGTCAAACAGTACCTCAACATGATGCCACATTTCTATCATTGTAC
CGTTGAACAAGCACGTCACGGACAAGATGCTGGGCTACTGGGTGCCGCTTGGTGGGTAGCCGATTGCCTAAAACAAGGCG
TTCATTTGAAATAG

Upstream 100 bases:

>100_bases
TGTCACCGTAGGATCTGCGCTGACTCGCCTAGAACATATCGTAAGCTGGTTTGCCGATGCCGTCAAATCGGCAAAATAAG
TCATCACAGAAGGAGAGCCT

Downstream 100 bases:

>100_bases
GAGAATCATATGTTTATTGGGGACTTAACAAGAAACGATTTCAAATTAGGCTTACCTCCTGTTATTGCTGAGGTATGTGA
CTATTTAAATACATTAGATT

Product: N-acetylmannosamine kinase

Products: NA

Alternate protein names: ManNAc kinase; N-acetyl-D-mannosamine kinase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRCLALDIGGTKIASAIVTDGKIEQRQQIATPQADAANAMHDTLANILALYAGQFDYVAVASTGIINHGVLTALNPKNLG
GLAEFPLKESIARHTDKPIGLLNDVQAAACAEYKDEDKNAVQNFVFITVSTGVGGGIILERRLLTEPNGVAGHIGHTLAD
PNGPVCGCGRVGCVEAVAAGRAIEAVSSQWNPPCTPKQAFELFRKNDEKATALIQRSASAIANLIADLVIGLDVQKVVVG
GSVGLAEGYLPLVKQYLNMMPHFYHCTVEQARHGQDAGLLGAAWWVADCLKQGVHLK

Sequences:

>Translated_297_residues
MRCLALDIGGTKIASAIVTDGKIEQRQQIATPQADAANAMHDTLANILALYAGQFDYVAVASTGIINHGVLTALNPKNLG
GLAEFPLKESIARHTDKPIGLLNDVQAAACAEYKDEDKNAVQNFVFITVSTGVGGGIILERRLLTEPNGVAGHIGHTLAD
PNGPVCGCGRVGCVEAVAAGRAIEAVSSQWNPPCTPKQAFELFRKNDEKATALIQRSASAIANLIADLVIGLDVQKVVVG
GSVGLAEGYLPLVKQYLNMMPHFYHCTVEQARHGQDAGLLGAAWWVADCLKQGVHLK
>Mature_297_residues
MRCLALDIGGTKIASAIVTDGKIEQRQQIATPQADAANAMHDTLANILALYAGQFDYVAVASTGIINHGVLTALNPKNLG
GLAEFPLKESIARHTDKPIGLLNDVQAAACAEYKDEDKNAVQNFVFITVSTGVGGGIILERRLLTEPNGVAGHIGHTLAD
PNGPVCGCGRVGCVEAVAAGRAIEAVSSQWNPPCTPKQAFELFRKNDEKATALIQRSASAIANLIADLVIGLDVQKVVVG
GSVGLAEGYLPLVKQYLNMMPHFYHCTVEQARHGQDAGLLGAAWWVADCLKQGVHLK

Specific function: Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family. NanK subfamily

Homologues:

Organism=Escherichia coli, GI87082230, Length=277, Percent_Identity=49.8194945848375, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1787363, Length=302, Percent_Identity=26.8211920529801, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI87081733, Length=298, Percent_Identity=24.8322147651007, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082127, Length=160, Percent_Identity=28.75, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787878, Length=224, Percent_Identity=25.4464285714286, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786891, Length=167, Percent_Identity=29.3413173652695, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NANK_PASMU (Q9CKB3)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246651.1
- ProteinModelPortal:   Q9CKB3
- PRIDE:   Q9CKB3
- GeneID:   1245059
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1712
- NMPDR:   fig|272843.1.peg.1713
- HOGENOM:   HBG730260
- OMA:   ICGCGRR
- ProtClustDB:   PRK05082
- BioCyc:   PMUL272843:PM1712-MONOMER
- BRENDA:   2.7.1.60
- HAMAP:   MF_01234
- InterPro:   IPR000600

Pfam domain/function: PF00480 ROK

EC number: =2.7.1.60

Molecular weight: Translated: 31351; Mature: 31351

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRCLALDIGGTKIASAIVTDGKIEQRQQIATPQADAANAMHDTLANILALYAGQFDYVAV
CEEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
ASTGIINHGVLTALNPKNLGGLAEFPLKESIARHTDKPIGLLNDVQAAACAEYKDEDKNA
ECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHH
VQNFVFITVSTGVGGGIILERRLLTEPNGVAGHIGHTLADPNGPVCGCGRVGCVEAVAAG
HHEEEEEEEECCCCCCCEEEHHHHCCCCCCCHHCCCCCCCCCCCEECCCCHHHHHHHHHC
RAIEAVSSQWNPPCTPKQAFELFRKNDEKATALIQRSASAIANLIADLVIGLDVQKVVVG
HHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEC
GSVGLAEGYLPLVKQYLNMMPHFYHCTVEQARHGQDAGLLGAAWWVADCLKQGVHLK
CCCCHHHCCHHHHHHHHHHCCHHEEEEHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRCLALDIGGTKIASAIVTDGKIEQRQQIATPQADAANAMHDTLANILALYAGQFDYVAV
CEEEEEECCCHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
ASTGIINHGVLTALNPKNLGGLAEFPLKESIARHTDKPIGLLNDVQAAACAEYKDEDKNA
ECCCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHH
VQNFVFITVSTGVGGGIILERRLLTEPNGVAGHIGHTLADPNGPVCGCGRVGCVEAVAAG
HHEEEEEEEECCCCCCCEEEHHHHCCCCCCCHHCCCCCCCCCCCEECCCCHHHHHHHHHC
RAIEAVSSQWNPPCTPKQAFELFRKNDEKATALIQRSASAIANLIADLVIGLDVQKVVVG
HHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEEC
GSVGLAEGYLPLVKQYLNMMPHFYHCTVEQARHGQDAGLLGAAWWVADCLKQGVHLK
CCCCHHHCCHHHHHHHHHHCCHHEEEEHHHHCCCCCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100