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The map label for this gene is dapF

Identifier: 15603568

GI number: 15603568

Start: 1909688

End: 1910512

Strand: Reverse

Name: dapF

Synonym: PM1703

Alternate gene names: 15603568

Gene position: 1910512-1909688 (Counterclockwise)

Preceding gene: 15603572

Following gene: 15603567

Centisome position: 84.63

GC content: 43.64

Gene sequence:

>825_bases
ATGCAATTTTCCAAAATGCACGGCTTAGGTAATGATTTTGTAGTAGTGGATGCCATTACACAAAACCTCTATTTTTCTCC
AGAAACCATTAAGCGTTTGGCAGACCGCCATCGTGGTATTGGTTTTGACCAAATGTTGATCGTTGAACCACCATATGATC
CTGATCTCGATTTTCATTATCGTATTTTTAATGCAGATGGCAGTGAAGTATCGCAATGCGGTAATGGCGCACGTTGTTTT
GCCCGTTTTGTGACGTTGAAAGGGTTGACCGATAAAAAAGACATTGCGGTCAGTACGCAAACAGGCAAAATGATTTTATC
AATTAAAGATGACGGCATGATCCGCATTAATATGGGAGAGCCGATTTGGGAACCTGCGAAAATCCCCTTTACTGCCAATA
AATTTGAAAAAAACTATATCTTACGTACCTCTATTCAGACGGTGTTATGTGGTGCCGTGTCGATGGGCAATCCCCATTGT
GTAGTGCAAGTGGACGATATTCAAACTGCCAATGTTGAGCAATTAGGACCTTTATTGGAAAACCACGAACGTTTTCCAGA
GCGCGTAAACGCTGGTTTTATGCAAGTGATTCATCGAGGACATATTAAATTACGTGTGTACGAGCGAGGCGCTGGTGAAA
CCCAAGCTTGCGGTAGTGGCGCCTGTGCTGCGGTGGCAGTGGGTGTGATGCAAGGATTATTGGACAGTAAAGTGCAAGTG
GATCTTCCAGGTGGGAGCTTGATTATTGAGTGGGAAGGCGTAGGTAAACCATTATTTATGACAGGTGATGCGACACACGT
GTATGATGGAGTGATTCGGTTATAG

Upstream 100 bases:

>100_bases
TTATGAGTTTTTGTGAGAGTCATCACGCTGTAATGATTTACTGATTTTTCTTGCCTGTTATAATGCCAGTCATTCAGCGT
TTCAATTAAAGGACAGGAAA

Downstream 100 bases:

>100_bases
TCATTTGTCACTTTTCTTTGCTTCGCCAAAGAAAAGTAACCAAAAGCCACCTACTTTCTTTTGCTTGTACAAAAGAAAGT
AGGCAAAGAAAATACACCCC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MQFSKMHGLGNDFVVVDAITQNLYFSPETIKRLADRHRGIGFDQMLIVEPPYDPDLDFHYRIFNADGSEVSQCGNGARCF
ARFVTLKGLTDKKDIAVSTQTGKMILSIKDDGMIRINMGEPIWEPAKIPFTANKFEKNYILRTSIQTVLCGAVSMGNPHC
VVQVDDIQTANVEQLGPLLENHERFPERVNAGFMQVIHRGHIKLRVYERGAGETQACGSGACAAVAVGVMQGLLDSKVQV
DLPGGSLIIEWEGVGKPLFMTGDATHVYDGVIRL

Sequences:

>Translated_274_residues
MQFSKMHGLGNDFVVVDAITQNLYFSPETIKRLADRHRGIGFDQMLIVEPPYDPDLDFHYRIFNADGSEVSQCGNGARCF
ARFVTLKGLTDKKDIAVSTQTGKMILSIKDDGMIRINMGEPIWEPAKIPFTANKFEKNYILRTSIQTVLCGAVSMGNPHC
VVQVDDIQTANVEQLGPLLENHERFPERVNAGFMQVIHRGHIKLRVYERGAGETQACGSGACAAVAVGVMQGLLDSKVQV
DLPGGSLIIEWEGVGKPLFMTGDATHVYDGVIRL
>Mature_274_residues
MQFSKMHGLGNDFVVVDAITQNLYFSPETIKRLADRHRGIGFDQMLIVEPPYDPDLDFHYRIFNADGSEVSQCGNGARCF
ARFVTLKGLTDKKDIAVSTQTGKMILSIKDDGMIRINMGEPIWEPAKIPFTANKFEKNYILRTSIQTVLCGAVSMGNPHC
VVQVDDIQTANVEQLGPLLENHERFPERVNAGFMQVIHRGHIKLRVYERGAGETQACGSGACAAVAVGVMQGLLDSKVQV
DLPGGSLIIEWEGVGKPLFMTGDATHVYDGVIRL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=73.7226277372263, Blast_Score=447, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_PASMU (P57962)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246642.1
- ProteinModelPortal:   P57962
- SMR:   P57962
- GeneID:   1245050
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1703
- NMPDR:   fig|272843.1.peg.1704
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- BioCyc:   PMUL272843:PM1703-MONOMER
- BRENDA:   5.1.1.7
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30184; Mature: 30184

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 73-73 ACT_SITE 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFSKMHGLGNDFVVVDAITQNLYFSPETIKRLADRHRGIGFDQMLIVEPPYDPDLDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEE
RIFNADGSEVSQCGNGARCFARFVTLKGLTDKKDIAVSTQTGKMILSIKDDGMIRINMGE
EEEECCCHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCEEEEECCC
PIWEPAKIPFTANKFEKNYILRTSIQTVLCGAVSMGNPHCVVQVDDIQTANVEQLGPLLE
CCCCCCCCCEECCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHH
NHERFPERVNAGFMQVIHRGHIKLRVYERGAGETQACGSGACAAVAVGVMQGLLDSKVQV
HHHHHHHHHCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
DLPGGSLIIEWEGVGKPLFMTGDATHVYDGVIRL
ECCCCEEEEEECCCCCCEEEECCCHHHHCCEECC
>Mature Secondary Structure
MQFSKMHGLGNDFVVVDAITQNLYFSPETIKRLADRHRGIGFDQMLIVEPPYDPDLDFHY
CCCHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEE
RIFNADGSEVSQCGNGARCFARFVTLKGLTDKKDIAVSTQTGKMILSIKDDGMIRINMGE
EEEECCCHHHHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCCCEEEEECCC
PIWEPAKIPFTANKFEKNYILRTSIQTVLCGAVSMGNPHCVVQVDDIQTANVEQLGPLLE
CCCCCCCCCEECCCCCCCEEEEHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHH
NHERFPERVNAGFMQVIHRGHIKLRVYERGAGETQACGSGACAAVAVGVMQGLLDSKVQV
HHHHHHHHHCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
DLPGGSLIIEWEGVGKPLFMTGDATHVYDGVIRL
ECCCCEEEEEECCCCCCEEEECCCHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100