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The map label for this gene is dhaK [H]

Identifier: 15603514

GI number: 15603514

Start: 1857366

End: 1858370

Strand: Reverse

Name: dhaK [H]

Synonym: PM1649

Alternate gene names: 15603514

Gene position: 1858370-1857366 (Counterclockwise)

Preceding gene: 15603515

Following gene: 15603513

Centisome position: 82.32

GC content: 41.79

Gene sequence:

>1005_bases
ATGACTAGAAAACCAAAAAAACTCCTTAATCATCCAAATGATGTGCCTGCGGAGCAATTAGAAGGGTTGCTCTATGCATG
CCATGGGAAACTTTCAAAAGTAGAGGGTTATAGTGGAGTGATTCGTACAGATATTGCAGATCAGCAAGTCGTCGTGGTTA
CTGGTGGTGGTAGTGGACATGAACCGATGTTTGCGGGCTTTGTGGGGAAAGGATTAGCAGACGCTGCGGCACTGGGTGAA
ATTTTTACTTCACCTTCTCCAGATATTATTATCGAAACGACTAAAGCCGCACAAAAAGGTCAAGGTGTGCTTTTCGTCTA
TGGTAATTATGCTGGGGATAATATGAATTTTGATATTGCGGCAGAATTATTAGAAGAAGAAGGTATCACTGTCAAAACCG
TACGTGTGACAGACGATATTTCTGCGGCACCGCCAGAAAGAATGCAAGAACGTCGTGGTGTTGCTGGTGATATGTATGTC
CTTAAAATTGCAGGTGCAGCCGTTCAGTCCGGTTATGACCTTGACGCCTTACATGAAGTGACGAGTAAAGCGAATTTTAA
TACTCGAACTATGGGGGTTGCTTTAGCGGCATGTTCTATTCCTCAGACAGGAAAATTTAACTTTGAGCTTGCAGATGATG
AATTAGAGCTTGGTATGGGGATTCATGGTGAAACGGGCGTAAGACGTCAGAAATTAACAAGTGCGGATGAAATTAACCAA
GAAATTGTTGATCGGATTTGTGCGGATATCGGGTTGCAAGCAGGAGATAGGGTGTGTGTCACAATCAATAATTTAGGTGC
GGCAACTTATACTGAGTTATTAATTTCAAACCGCAAAGTCCATCAAGAATTAGCAAAACGTGGTATTCAAGTGCATGACA
CTTTGATTGGTTCTTATTGTACGTCTCAGGAAATGGCGGGGTATTCTGTGACACTTTTCCGCTTAGATGATGAATTACAA
AAATTATATGACTTGCCTTGTGATGGTTTTGCATGGAGAAAATAA

Upstream 100 bases:

>100_bases
ATATTGCTATTCCGACTGCATTTGAAGCAGCGTTTAACATTGCAGTTAGAATGGGACAGGGTGTTTTAAATCAAAAGTAA
TCTTTGCTGAGGACTCAAAA

Downstream 100 bases:

>100_bases
AATGAATGTAATTGAAACAAAAGCATTATTGCTATATACCGCAGAGAAAATGATGGCAAGCGAGCCAACACTTACTGAAC
TTGATCTCAAGATCGGTGAT

Product: hypothetical protein

Products: ADP; glycerone phosphate

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTRKPKKLLNHPNDVPAEQLEGLLYACHGKLSKVEGYSGVIRTDIADQQVVVVTGGGSGHEPMFAGFVGKGLADAAALGE
IFTSPSPDIIIETTKAAQKGQGVLFVYGNYAGDNMNFDIAAELLEEEGITVKTVRVTDDISAAPPERMQERRGVAGDMYV
LKIAGAAVQSGYDLDALHEVTSKANFNTRTMGVALAACSIPQTGKFNFELADDELELGMGIHGETGVRRQKLTSADEINQ
EIVDRICADIGLQAGDRVCVTINNLGAATYTELLISNRKVHQELAKRGIQVHDTLIGSYCTSQEMAGYSVTLFRLDDELQ
KLYDLPCDGFAWRK

Sequences:

>Translated_334_residues
MTRKPKKLLNHPNDVPAEQLEGLLYACHGKLSKVEGYSGVIRTDIADQQVVVVTGGGSGHEPMFAGFVGKGLADAAALGE
IFTSPSPDIIIETTKAAQKGQGVLFVYGNYAGDNMNFDIAAELLEEEGITVKTVRVTDDISAAPPERMQERRGVAGDMYV
LKIAGAAVQSGYDLDALHEVTSKANFNTRTMGVALAACSIPQTGKFNFELADDELELGMGIHGETGVRRQKLTSADEINQ
EIVDRICADIGLQAGDRVCVTINNLGAATYTELLISNRKVHQELAKRGIQVHDTLIGSYCTSQEMAGYSVTLFRLDDELQ
KLYDLPCDGFAWRK
>Mature_333_residues
TRKPKKLLNHPNDVPAEQLEGLLYACHGKLSKVEGYSGVIRTDIADQQVVVVTGGGSGHEPMFAGFVGKGLADAAALGEI
FTSPSPDIIIETTKAAQKGQGVLFVYGNYAGDNMNFDIAAELLEEEGITVKTVRVTDDISAAPPERMQERRGVAGDMYVL
KIAGAAVQSGYDLDALHEVTSKANFNTRTMGVALAACSIPQTGKFNFELADDELELGMGIHGETGVRRQKLTSADEINQE
IVDRICADIGLQAGDRVCVTINNLGAATYTELLISNRKVHQELAKRGIQVHDTLIGSYCTSQEMAGYSVTLFRLDDELQK
LYDLPCDGFAWRK

Specific function: Dihydroxyacetone binding subunit of the dihydroxyacetone kinase, which is responsible for phosphorylating dihydroxyacetone. Binds covalently dihydroxyacetone in hemiaminal linkage. Acts also as a corepressor of dhaR by binding to its sensor domain, in the

COG id: COG2376

COG function: function code G; Dihydroxyacetone kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DhaK domain [H]

Homologues:

Organism=Homo sapiens, GI20149621, Length=339, Percent_Identity=37.7581120943953, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI87081857, Length=346, Percent_Identity=39.3063583815029, Blast_Score=244, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI17565018, Length=334, Percent_Identity=35.0299401197605, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321055, Length=298, Percent_Identity=32.8859060402685, Blast_Score=150, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6323570, Length=281, Percent_Identity=32.7402135231317, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004006
- InterPro:   IPR012736 [H]

Pfam domain/function: PF02733 Dak1 [H]

EC number: 2.7.1.29

Molecular weight: Translated: 36101; Mature: 35970

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKPKKLLNHPNDVPAEQLEGLLYACHGKLSKVEGYSGVIRTDIADQQVVVVTGGGSGH
CCCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCEEEECCCCCEEEEEECCCCCC
EPMFAGFVGKGLADAAALGEIFTSPSPDIIIETTKAAQKGQGVLFVYGNYAGDNMNFDIA
CCHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCEEEEEECCCCCCCCHHHH
AELLEEEGITVKTVRVTDDISAAPPERMQERRGVAGDMYVLKIAGAAVQSGYDLDALHEV
HHHHHHCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEECHHHHCCCCHHHHHHH
TSKANFNTRTMGVALAACSIPQTGKFNFELADDELELGMGIHGETGVRRQKLTSADEINQ
HHCCCCCCHHHHHEEEEECCCCCCCEEEEECCCHHHHCCCCCCCCCCHHHHCCCHHHHHH
EIVDRICADIGLQAGDRVCVTINNLGAATYTELLISNRKVHQELAKRGIQVHDTLIGSYC
HHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCEEEHHHHHHHH
TSQEMAGYSVTLFRLDDELQKLYDLPCDGFAWRK
CCCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TRKPKKLLNHPNDVPAEQLEGLLYACHGKLSKVEGYSGVIRTDIADQQVVVVTGGGSGH
CCCCHHHHCCCCCCCHHHHHHHHHHHCCCHHHCCCCCCEEEECCCCCEEEEEECCCCCC
EPMFAGFVGKGLADAAALGEIFTSPSPDIIIETTKAAQKGQGVLFVYGNYAGDNMNFDIA
CCHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCHHHCCCCEEEEEECCCCCCCCHHHH
AELLEEEGITVKTVRVTDDISAAPPERMQERRGVAGDMYVLKIAGAAVQSGYDLDALHEV
HHHHHHCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEECHHHHCCCCHHHHHHH
TSKANFNTRTMGVALAACSIPQTGKFNFELADDELELGMGIHGETGVRRQKLTSADEINQ
HHCCCCCCHHHHHEEEEECCCCCCCEEEEECCCHHHHCCCCCCCCCCHHHHCCCHHHHHH
EIVDRICADIGLQAGDRVCVTINNLGAATYTELLISNRKVHQELAKRGIQVHDTLIGSYC
HHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHHCCCEEEHHHHHHHH
TSQEMAGYSVTLFRLDDELQKLYDLPCDGFAWRK
CCCCCCCCEEEEEEECHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; glycerone

Specific reaction: ATP + glycerone = ADP + glycerone phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]