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The map label for this gene is napB [H]

Identifier: 15603462

GI number: 15603462

Start: 1808571

End: 1808996

Strand: Direct

Name: napB [H]

Synonym: PM1597

Alternate gene names: 15603462

Gene position: 1808571-1808996 (Clockwise)

Preceding gene: 15603461

Following gene: 15603463

Centisome position: 80.11

GC content: 42.96

Gene sequence:

>426_bases
ATGAAAAAAACAATGCTAATTTTGACCGCACTTTTTGCCTTCACGGTCAATGCCAATGAAGTCAAAGTGGGCAAAAGTTT
ACAAGATTCACCTGAAAATATCGCGCCAGCCTTCCACAATACACCAAAAGAAAGTGGCTTGGCGCCGTTAAACTATGTGA
ACCAACCACCAATGGTGCCACACGCGACGAAAAACTATCAGGTGACGAAAAATATCAACCAATGTTTAACTTGTCATAGC
CCAGAAGCCTCACGTGTTACTGGTGCAACACGCATCAGTCCAACCCACTTTATGGATCGTGATGGTAACATTGTCGGAGG
CACCTCACCTCGCCGTTACTTCTGTTTACAATGCCATGTCTCTCAATCTGATGTTGAGCCGATTATTCAAAATGAATTTA
AACCGATGGCTGGTTTTGGTAAATAA

Upstream 100 bases:

>100_bases
GTATCAGCTGTGGACGTTGTATCGACGTTTGCCCTGAAAAAGTATTTATTTTTACAACACGATTTAATCATTCAGTTAAT
CATTCGGGGGAGTGATCAAA

Downstream 100 bases:

>100_bases
GTTAAGGACGGAACTATGTTGAACTTAATTAAACGCTTTTGGAAATGGTTTCGCTCGCCCAGTCGTATTGCCGTTGGTAC
CTTGATTACCCTTGCTTTTA

Product: NapB

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 141; Mature: 141

Protein sequence:

>141_residues
MKKTMLILTALFAFTVNANEVKVGKSLQDSPENIAPAFHNTPKESGLAPLNYVNQPPMVPHATKNYQVTKNINQCLTCHS
PEASRVTGATRISPTHFMDRDGNIVGGTSPRRYFCLQCHVSQSDVEPIIQNEFKPMAGFGK

Sequences:

>Translated_141_residues
MKKTMLILTALFAFTVNANEVKVGKSLQDSPENIAPAFHNTPKESGLAPLNYVNQPPMVPHATKNYQVTKNINQCLTCHS
PEASRVTGATRISPTHFMDRDGNIVGGTSPRRYFCLQCHVSQSDVEPIIQNEFKPMAGFGK
>Mature_141_residues
MKKTMLILTALFAFTVNANEVKVGKSLQDSPENIAPAFHNTPKESGLAPLNYVNQPPMVPHATKNYQVTKNINQCLTCHS
PEASRVTGATRISPTHFMDRDGNIVGGTSPRRYFCLQCHVSQSDVEPIIQNEFKPMAGFGK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=144, Percent_Identity=50.6944444444444, Blast_Score=149, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 15531; Mature: 15531

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTMLILTALFAFTVNANEVKVGKSLQDSPENIAPAFHNTPKESGLAPLNYVNQPPMVP
CCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HATKNYQVTKNINQCLTCHSPEASRVTGATRISPTHFMDRDGNIVGGTSPRRYFCLQCHV
CCCCCEEEECCHHHHEECCCCCCCCCCCCCCCCCCEEECCCCCEECCCCCCEEEEEEEEC
SQSDVEPIIQNEFKPMAGFGK
CCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKTMLILTALFAFTVNANEVKVGKSLQDSPENIAPAFHNTPKESGLAPLNYVNQPPMVP
CCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
HATKNYQVTKNINQCLTCHSPEASRVTGATRISPTHFMDRDGNIVGGTSPRRYFCLQCHV
CCCCCEEEECCHHHHEECCCCCCCCCCCCCCCCCCEEECCCCCEECCCCCCEEEEEEEEC
SQSDVEPIIQNEFKPMAGFGK
CCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 11389694; 11223519 [H]