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The map label for this gene is napA

Identifier: 15603459

GI number: 15603459

Start: 1804388

End: 1806874

Strand: Direct

Name: napA

Synonym: PM1594

Alternate gene names: 15603459

Gene position: 1804388-1806874 (Clockwise)

Preceding gene: 15603458

Following gene: 15603460

Centisome position: 79.93

GC content: 43.99

Gene sequence:

>2487_bases
ATGAATTTAAGTCGTCGTGACTTTATGAAAACCAATGCGGCGGTTGCAGCTGCAGCGGTCGCTGGCTTGGCTATTCCAGT
TAAGAATGTGGAAGCGTCGCCAGATAAAATTATTAAATGGGATAAAGCGCCTTGCCGTTTTTGTGGTACGGGTTGTAGCG
TGTTAGTGGGGACACAAAATGGACGCGTTGTTGCTTCGCAAGGAGATCCTGATGCGGATGTGAACCGTGGGTTGAACTGT
ATTAAAGGTTATTTCTTACCAAAAATCATGTACGGTAAAGATCGCTTAACTTCCCCGATGTTACGGATGACAAACGGTAA
GTTTGATAAACACGGCGAATTTACTCCGGTGTCTTGGGATCAAGCCTTTACGATCATGGCAGAAAAATTCAAAAAAGCCT
TGAAAGAAAAAGGACCAAATGGGGCAGGCATGTTCACTTCTGGACAATCTACCATTTTTGAAGGGATTGCCAAATCCAAA
TTGTTTAAAGCGGGACTCCGTTCAAATAATATTGACCCGAATGCACGCCATTGTATGGCATCAGCAGCGGTTGCCTTTAT
GCGTACTTTTGGTATGGATGAGCCAATGGGGTGTTATGATGACATCGAAAAAGCCGATGCATTTGTGTTGTGGGGATCAA
ATATGGCAGAAATGCACCCGATTTTGTGGTCGCGTATTTCGGATCGCCGTTTATCTCATCCAGATGTGAAAGTCGCTGTC
TTATCTACTTTTGAGCACCGTTCTTTTGAATTAGCTGATTTGGGTGTCATTTTTACGCCACAATCTGATTTGGCGATCAT
GAACTACATTGCCAATTACCTGATTCAACATGATGCGATTGATCATGACTTTATTCAAAAACACACCAAATTCAAGCGTG
GTGAAACAGATATCGGTTATGGCTTGCGTGAAACCCACGAATTAGAAAAAGCAGCGAAAAACGTGAAAACTGCGGGTAAA
ATGCACGACAGTGATTTCGAAGAATATAAAAAATTAGTCGCACCATACACCTTAGAAAAAGCCCATGAAATTTCAGGGGT
ACCGAAAGAACAACTTGAAGCACTGGCTAAGATGTATGCCGATCCAAAACTGAATATCGTGTCTTTCTGGACCATGGGTT
TCAACCAACATACACGTGGTGTGTGGGCAAACCACTTAATCTATAACATTCATTTATTAACCGGCAAAATCTCTAAACCA
GGGTGTGGACCGTTCTCGCTAACGGGTCAACCTTCTGCCTGTGGTACCGCACGTGAGGTCGGCACCTTTGCACACCGTTT
ACCCGCAGATTTGGTGGTCACTAATCCTAAACACGTTGAAAAAGCCGAGAAACTGTGGAAGTTGCCAAAAGGCGTGATTC
AAACTCAAGTAGGTTATCACGCGGTAGCACAAGACCGTGCGTTAAAAGATAAGAAAATGAATGTGTTATGGCAAATGTGT
ACCAATAACATGCAAGGCGGTCCAAACATCAACCAAGAGCGTTTCCCGGGTTGGCGTGACGAAGAAAACTTTGTGATTGT
ATCTGATCCTTATCCAACGGTTTCTGCTCTTGCCGCTGACTTAATTTTACCGACAGCGATGTGGGTAGAAAAAGAAGGCG
CTTATGGTAACGCAGAACGCCGTACGCAATTCTGGTATCAACAAGTCAAAGCACCGGGTGAGTCAAAATCGGACGTGTGG
CAATTAGTGGAGTTTTCTAAATATTTCACTACCGATGAAATGTGGCCAGCGGAAGTACTTGCGGCGAACCCTGAATATAA
AGGTAAAACCTTGTATGAAGTGTTATTCCGTAATGGTCAAGTGGATAAATTCCAAGTACCAACGGATAAACCAGGTTACA
TGAATGATGAAGCAGAACATTTTGGCTTCTATTTACAAAAAGGCTTGTTTGAAGAGTATGCCGTGTTTGGTCGCGGACAT
GGTCATGATTTAGCAGACTTTGAAACTTACCATAAAGCACGTGGCTTACGCTGGCCAGTGGTGGATGGCAAAGAAACCTT
ATGGCGTTATCGTGAAGGTTATGACCCTTACGTCAAAGAAGGCGAAGGGGTGGCGTTCTATGGCTATCCAGACAAAAAAG
CAATTATTCTTGCCGTTCCTTATGAGCCACCAGCAGAAGCACCGAATGCGGAATATGACTTGTGGTTAACGACGGGGCGG
GTCTTAGAACATTGGCATACGGGTACGATGACACGCCGTGTCCCAGAGCTCCATCGCTCCTTCCCAAATAACTTGGTTTG
GATGCACCCATTAGATGCGAAAAAACGTGGTTTACGTCATGGTGATAAAGTGAAGATCAGCTCACGTCGTGGCGAAATGA
TTTCTCACTTAGATACCCGTGGACGTAATAAAGTCCCACAAGGCTTAGTTTACACCACTTTCTTTGATGCAGGTCAGTTA
GCAAACTATCTCACTTTAGATGCGACAGACCCAATTTCAAAAGAAACGGACTTTAAAAAATGTGCGGTCAAAGTGGAAAA
AGCGTAA

Upstream 100 bases:

>100_bases
GATATTGACGGCGTCATTGACGTGTCCTTGGTTTACCATCAACAAGACGAAAACGAATAAAACATCACATAATTCAATAT
AGTGGGGGAACGCTATGAAG

Downstream 100 bases:

>100_bases
CACGTTAAATTTAATGAGGAACGACCGCACTTTGCTTTCAGTAAAGTGCGGTTGGAAAGTCGAATGAAAAAACCAGTTGT
GAATCCTGAACGTCGTCGAT

Product: nitrate reductase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 828; Mature: 828

Protein sequence:

>828_residues
MNLSRRDFMKTNAAVAAAAVAGLAIPVKNVEASPDKIIKWDKAPCRFCGTGCSVLVGTQNGRVVASQGDPDADVNRGLNC
IKGYFLPKIMYGKDRLTSPMLRMTNGKFDKHGEFTPVSWDQAFTIMAEKFKKALKEKGPNGAGMFTSGQSTIFEGIAKSK
LFKAGLRSNNIDPNARHCMASAAVAFMRTFGMDEPMGCYDDIEKADAFVLWGSNMAEMHPILWSRISDRRLSHPDVKVAV
LSTFEHRSFELADLGVIFTPQSDLAIMNYIANYLIQHDAIDHDFIQKHTKFKRGETDIGYGLRETHELEKAAKNVKTAGK
MHDSDFEEYKKLVAPYTLEKAHEISGVPKEQLEALAKMYADPKLNIVSFWTMGFNQHTRGVWANHLIYNIHLLTGKISKP
GCGPFSLTGQPSACGTAREVGTFAHRLPADLVVTNPKHVEKAEKLWKLPKGVIQTQVGYHAVAQDRALKDKKMNVLWQMC
TNNMQGGPNINQERFPGWRDEENFVIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWYQQVKAPGESKSDVW
QLVEFSKYFTTDEMWPAEVLAANPEYKGKTLYEVLFRNGQVDKFQVPTDKPGYMNDEAEHFGFYLQKGLFEEYAVFGRGH
GHDLADFETYHKARGLRWPVVDGKETLWRYREGYDPYVKEGEGVAFYGYPDKKAIILAVPYEPPAEAPNAEYDLWLTTGR
VLEHWHTGTMTRRVPELHRSFPNNLVWMHPLDAKKRGLRHGDKVKISSRRGEMISHLDTRGRNKVPQGLVYTTFFDAGQL
ANYLTLDATDPISKETDFKKCAVKVEKA

Sequences:

>Translated_828_residues
MNLSRRDFMKTNAAVAAAAVAGLAIPVKNVEASPDKIIKWDKAPCRFCGTGCSVLVGTQNGRVVASQGDPDADVNRGLNC
IKGYFLPKIMYGKDRLTSPMLRMTNGKFDKHGEFTPVSWDQAFTIMAEKFKKALKEKGPNGAGMFTSGQSTIFEGIAKSK
LFKAGLRSNNIDPNARHCMASAAVAFMRTFGMDEPMGCYDDIEKADAFVLWGSNMAEMHPILWSRISDRRLSHPDVKVAV
LSTFEHRSFELADLGVIFTPQSDLAIMNYIANYLIQHDAIDHDFIQKHTKFKRGETDIGYGLRETHELEKAAKNVKTAGK
MHDSDFEEYKKLVAPYTLEKAHEISGVPKEQLEALAKMYADPKLNIVSFWTMGFNQHTRGVWANHLIYNIHLLTGKISKP
GCGPFSLTGQPSACGTAREVGTFAHRLPADLVVTNPKHVEKAEKLWKLPKGVIQTQVGYHAVAQDRALKDKKMNVLWQMC
TNNMQGGPNINQERFPGWRDEENFVIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWYQQVKAPGESKSDVW
QLVEFSKYFTTDEMWPAEVLAANPEYKGKTLYEVLFRNGQVDKFQVPTDKPGYMNDEAEHFGFYLQKGLFEEYAVFGRGH
GHDLADFETYHKARGLRWPVVDGKETLWRYREGYDPYVKEGEGVAFYGYPDKKAIILAVPYEPPAEAPNAEYDLWLTTGR
VLEHWHTGTMTRRVPELHRSFPNNLVWMHPLDAKKRGLRHGDKVKISSRRGEMISHLDTRGRNKVPQGLVYTTFFDAGQL
ANYLTLDATDPISKETDFKKCAVKVEKA
>Mature_828_residues
MNLSRRDFMKTNAAVAAAAVAGLAIPVKNVEASPDKIIKWDKAPCRFCGTGCSVLVGTQNGRVVASQGDPDADVNRGLNC
IKGYFLPKIMYGKDRLTSPMLRMTNGKFDKHGEFTPVSWDQAFTIMAEKFKKALKEKGPNGAGMFTSGQSTIFEGIAKSK
LFKAGLRSNNIDPNARHCMASAAVAFMRTFGMDEPMGCYDDIEKADAFVLWGSNMAEMHPILWSRISDRRLSHPDVKVAV
LSTFEHRSFELADLGVIFTPQSDLAIMNYIANYLIQHDAIDHDFIQKHTKFKRGETDIGYGLRETHELEKAAKNVKTAGK
MHDSDFEEYKKLVAPYTLEKAHEISGVPKEQLEALAKMYADPKLNIVSFWTMGFNQHTRGVWANHLIYNIHLLTGKISKP
GCGPFSLTGQPSACGTAREVGTFAHRLPADLVVTNPKHVEKAEKLWKLPKGVIQTQVGYHAVAQDRALKDKKMNVLWQMC
TNNMQGGPNINQERFPGWRDEENFVIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWYQQVKAPGESKSDVW
QLVEFSKYFTTDEMWPAEVLAANPEYKGKTLYEVLFRNGQVDKFQVPTDKPGYMNDEAEHFGFYLQKGLFEEYAVFGRGH
GHDLADFETYHKARGLRWPVVDGKETLWRYREGYDPYVKEGEGVAFYGYPDKKAIILAVPYEPPAEAPNAEYDLWLTTGR
VLEHWHTGTMTRRVPELHRSFPNNLVWMHPLDAKKRGLRHGDKVKISSRRGEMISHLDTRGRNKVPQGLVYTTFFDAGQL
ANYLTLDATDPISKETDFKKCAVKVEKA

Specific function: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and peripla

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily

Homologues:

Organism=Escherichia coli, GI1788534, Length=828, Percent_Identity=74.0338164251208, Blast_Score=1291, Evalue=0.0,
Organism=Escherichia coli, GI3868721, Length=793, Percent_Identity=23.7074401008827, Blast_Score=199, Evalue=5e-52,
Organism=Escherichia coli, GI3868719, Length=505, Percent_Identity=23.960396039604, Blast_Score=116, Evalue=6e-27,
Organism=Escherichia coli, GI3868720, Length=505, Percent_Identity=23.7623762376238, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI87081797, Length=883, Percent_Identity=22.7633069082673, Blast_Score=94, Evalue=5e-20,
Organism=Escherichia coli, GI1787870, Length=557, Percent_Identity=21.7235188509874, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAPA_PASMU (Q9CKL8)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246533.1
- ProteinModelPortal:   Q9CKL8
- SMR:   Q9CKL8
- GeneID:   1244941
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1594
- NMPDR:   fig|272843.1.peg.1595
- HOGENOM:   HBG715033
- OMA:   IISAPYE
- ProtClustDB:   PRK13532
- BioCyc:   PMUL272843:PM1594-MONOMER
- BRENDA:   1.7.99.4
- GO:   GO:0006810
- HAMAP:   MF_01630
- InterPro:   IPR009010
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR006655
- InterPro:   IPR010051
- InterPro:   IPR006311
- Gene3D:   G3DSA:2.40.40.20
- SMART:   SM00926
- TIGRFAMs:   TIGR01706
- TIGRFAMs:   TIGR01409

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; SSF50692 Asp_decarb_fold

EC number: =1.7.99.4

Molecular weight: Translated: 93356; Mature: 93356

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00551 MOLYBDOPTERIN_PROK_1; PS00490 MOLYBDOPTERIN_PROK_2; PS00932 MOLYBDOPTERIN_PROK_3; PS51318 TAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSRRDFMKTNAAVAAAAVAGLAIPVKNVEASPDKIIKWDKAPCRFCGTGCSVLVGTQN
CCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCHHECCCCCEEEEECCC
GRVVASQGDPDADVNRGLNCIKGYFLPKIMYGKDRLTSPMLRMTNGKFDKHGEFTPVSWD
CEEEECCCCCCHHHHHCHHHHHHHHHHHHEECCHHHCCHHHHHCCCCCCCCCCCCCCCHH
QAFTIMAEKFKKALKEKGPNGAGMFTSGQSTIFEGIAKSKLFKAGLRSNNIDPNARHCMA
HHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SAAVAFMRTFGMDEPMGCYDDIEKADAFVLWGSNMAEMHPILWSRISDRRLSHPDVKVAV
HHHHHHHHHHCCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEE
LSTFEHRSFELADLGVIFTPQSDLAIMNYIANYLIQHDAIDHDFIQKHTKFKRGETDIGY
EEHHCCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
GLRETHELEKAAKNVKTAGKMHDSDFEEYKKLVAPYTLEKAHEISGVPKEQLEALAKMYA
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHC
DPKLNIVSFWTMGFNQHTRGVWANHLIYNIHLLTGKISKPGCGPFSLTGQPSACGTAREV
CCCCEEEEEEECCCCCCCCCHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
GTFAHRLPADLVVTNPKHVEKAEKLWKLPKGVIQTQVGYHAVAQDRALKDKKMNVLWQMC
HHHHHHCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNNMQGGPNINQERFPGWRDEENFVIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAER
HCCCCCCCCCCHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCHHH
RTQFWYQQVKAPGESKSDVWQLVEFSKYFTTDEMWPAEVLAANPEYKGKTLYEVLFRNGQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCC
VDKFQVPTDKPGYMNDEAEHFGFYLQKGLFEEYAVFGRGHGHDLADFETYHKARGLRWPV
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC
VDGKETLWRYREGYDPYVKEGEGVAFYGYPDKKAIILAVPYEPPAEAPNAEYDLWLTTGR
CCCHHHHHHHHCCCCCHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEHHH
VLEHWHTGTMTRRVPELHRSFPNNLVWMHPLDAKKRGLRHGDKVKISSRRGEMISHLDTR
HHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCCEEEECCHHHHHHHHHHHC
GRNKVPQGLVYTTFFDAGQLANYLTLDATDPISKETDFKKCAVKVEKA
CCCCCCCCEEEEEECCHHHHCCEEEECCCCCCCCCCCHHHHHEEECCC
>Mature Secondary Structure
MNLSRRDFMKTNAAVAAAAVAGLAIPVKNVEASPDKIIKWDKAPCRFCGTGCSVLVGTQN
CCCCHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCHHECCCCCEEEEECCC
GRVVASQGDPDADVNRGLNCIKGYFLPKIMYGKDRLTSPMLRMTNGKFDKHGEFTPVSWD
CEEEECCCCCCHHHHHCHHHHHHHHHHHHEECCHHHCCHHHHHCCCCCCCCCCCCCCCHH
QAFTIMAEKFKKALKEKGPNGAGMFTSGQSTIFEGIAKSKLFKAGLRSNNIDPNARHCMA
HHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
SAAVAFMRTFGMDEPMGCYDDIEKADAFVLWGSNMAEMHPILWSRISDRRLSHPDVKVAV
HHHHHHHHHHCCCCCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCEEEEE
LSTFEHRSFELADLGVIFTPQSDLAIMNYIANYLIQHDAIDHDFIQKHTKFKRGETDIGY
EEHHCCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCC
GLRETHELEKAAKNVKTAGKMHDSDFEEYKKLVAPYTLEKAHEISGVPKEQLEALAKMYA
CHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHC
DPKLNIVSFWTMGFNQHTRGVWANHLIYNIHLLTGKISKPGCGPFSLTGQPSACGTAREV
CCCCEEEEEEECCCCCCCCCHHHCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHH
GTFAHRLPADLVVTNPKHVEKAEKLWKLPKGVIQTQVGYHAVAQDRALKDKKMNVLWQMC
HHHHHHCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TNNMQGGPNINQERFPGWRDEENFVIVSDPYPTVSALAADLILPTAMWVEKEGAYGNAER
HCCCCCCCCCCHHHCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHEECCCCCCCHHH
RTQFWYQQVKAPGESKSDVWQLVEFSKYFTTDEMWPAEVLAANPEYKGKTLYEVLFRNGQ
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCC
VDKFQVPTDKPGYMNDEAEHFGFYLQKGLFEEYAVFGRGHGHDLADFETYHKARGLRWPV
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCC
VDGKETLWRYREGYDPYVKEGEGVAFYGYPDKKAIILAVPYEPPAEAPNAEYDLWLTTGR
CCCHHHHHHHHCCCCCHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEEEEEEHHH
VLEHWHTGTMTRRVPELHRSFPNNLVWMHPLDAKKRGLRHGDKVKISSRRGEMISHLDTR
HHHHHCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHCCCCCCEEEECCHHHHHHHHHHHC
GRNKVPQGLVYTTFFDAGQLANYLTLDATDPISKETDFKKCAVKVEKA
CCCCCCCCEEEEEECCHHHHCCEEEECCCCCCCCCCCHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100