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The map label for this gene is mscL

Identifier: 15603429

GI number: 15603429

Start: 1772223

End: 1772624

Strand: Direct

Name: mscL

Synonym: PM1564

Alternate gene names: 15603429

Gene position: 1772223-1772624 (Clockwise)

Preceding gene: 15603428

Following gene: 15603430

Centisome position: 78.5

GC content: 38.81

Gene sequence:

>402_bases
ATGAGTTTTGTTAAAGAGTTTCGCGAATTTGCAATGCGAGGCAATGTTGTCGATATGGCAGTCGGTGTGATCATCGGTGG
TGCATTTGGTAAAATTGTCAGTTCTTTAGTTGGTGATGTGGTGATGCCAGTATTAGGTATTTTAACTGGTGGTGTGGATT
TCAAAGATTTAAGTATTGTGTTGAAAGAAGCAGCGGGTGAAGTGCCTGCAGTCACGTTAAATTACGGTGCATTTATCCAA
ACAGTCTTCGACTTCGTCATTATTGCCTTTGCGATTTTCTTGATGATCAAAGCGTTAAATAAATTGAAACGTGAAGAGCC
AAAAGTAGAAGAACCTGCAGAGCCAAAACTATCAAACGAAGAAGTCCTATTAACGGAAATTCGCGATTTATTGAAGAAAT
AA

Upstream 100 bases:

>100_bases
AGTGTATTCTTTATTTAAAGTAGCGTAAATAAACTGTACAAAAAATCAAAAGTTCTTATAATGGCTAGGATTTTAGCTAA
CATTTTATTTAGGAGTTTTT

Downstream 100 bases:

>100_bases
TCGTTTTGACTCGTTGAAAAATCTTCCCTAGTGGAAGATTTTTTTATTTCAACCGGGCTCCAATCGTCGCAACTTGTATA
AGCCATTTATACCCTTTACA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 133; Mature: 132

Protein sequence:

>133_residues
MSFVKEFREFAMRGNVVDMAVGVIIGGAFGKIVSSLVGDVVMPVLGILTGGVDFKDLSIVLKEAAGEVPAVTLNYGAFIQ
TVFDFVIIAFAIFLMIKALNKLKREEPKVEEPAEPKLSNEEVLLTEIRDLLKK

Sequences:

>Translated_133_residues
MSFVKEFREFAMRGNVVDMAVGVIIGGAFGKIVSSLVGDVVMPVLGILTGGVDFKDLSIVLKEAAGEVPAVTLNYGAFIQ
TVFDFVIIAFAIFLMIKALNKLKREEPKVEEPAEPKLSNEEVLLTEIRDLLKK
>Mature_132_residues
SFVKEFREFAMRGNVVDMAVGVIIGGAFGKIVSSLVGDVVMPVLGILTGGVDFKDLSIVLKEAAGEVPAVTLNYGAFIQT
VFDFVIIAFAIFLMIKALNKLKREEPKVEEPAEPKLSNEEVLLTEIRDLLKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=134, Percent_Identity=67.1641791044776, Blast_Score=179, Evalue=5e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_PASMU (P57950)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246503.1
- ProteinModelPortal:   P57950
- SMR:   P57950
- GeneID:   1244911
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1564
- NMPDR:   fig|272843.1.peg.1565
- HOGENOM:   HBG727604
- OMA:   IQLLTEI
- ProtClustDB:   PRK00567
- BioCyc:   PMUL272843:PM1564-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14516; Mature: 14385

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xde7dc0c)-; HASH(0xf0bd8c4)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVKEFREFAMRGNVVDMAVGVIIGGAFGKIVSSLVGDVVMPVLGILTGGVDFKDLSIV
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKEAAGEVPAVTLNYGAFIQTVFDFVIIAFAIFLMIKALNKLKREEPKVEEPAEPKLSNE
HHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EVLLTEIRDLLKK
CHHHHHHHHHHCH
>Mature Secondary Structure 
SFVKEFREFAMRGNVVDMAVGVIIGGAFGKIVSSLVGDVVMPVLGILTGGVDFKDLSIV
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKEAAGEVPAVTLNYGAFIQTVFDFVIIAFAIFLMIKALNKLKREEPKVEEPAEPKLSNE
HHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EVLLTEIRDLLKK
CHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11248100