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The map label for this gene is tnaA [H]

Identifier: 15603285

GI number: 15603285

Start: 1602677

End: 1604092

Strand: Reverse

Name: tnaA [H]

Synonym: PM1420

Alternate gene names: 15603285

Gene position: 1604092-1602677 (Counterclockwise)

Preceding gene: 15603289

Following gene: 15603284

Centisome position: 71.06

GC content: 42.23

Gene sequence:

>1416_bases
ATGGAAAACTTCAAACATTTACCAGAGCCGTTTCGTATTCGTGTGATTGAGCCAGTGAGAAGAACCACGCGTTCGTACCG
TGAAGAAGCCATTTTAAAGGCGGGAATGAACCCATTCTTACTAGACAGTGAAGATGTGTTTATCGATCTCTTAACTGACA
GTGGAACAGGTGCAGTAACACAGGAAATGCAAGCGGCGATGTTACGTGGCGATGAAGCATACAGTGGTAGTCGTAGCTAT
CATGCGTTAGCGAATGCGGTGAAAGAGATTTTTGGTTATGAATATACAATTCCAACTCACCAAGGTCGTGGTGCAGAACA
AATTTATATTCCCGTATTAATTAAAAAGCGCGAGCAAGAAAAAGGCTTAGATCGTAGCAAAATGGTGGTGTTCTCTAACT
ATTTCTTCGATACCACACAAGGTCATAGCCAAATTAATGGTGCGACAGTACGCAACGTTTATACAAAAGAAGCGTTTGAT
ACCAGTGTTAACGCCGATTTCAAAGGGGATTTTGATCTTGAAAAATTAGAACAAGGGATTCAAGAAGTCGGTGCTGAAAA
CGTCCCATATATTGTTTGCACCATTACGTGTAACTCTGCGGGTGGTCAGCCTGTATCATTAGCCAATATGCGAGCGATGT
ATGAGATTGCGAAAAAATATGATATTCCAGTCGTTATGGATTCTGCCCGTTTTGCAGAAAATGCCTATTTCATCCAACAA
CGTGAACCTGGCTATAAAGAGTGGACGATCGAACAAATCACTTATGAAAGCTATAAATATGCTGATGCTTTAGCGATGTC
TGCGAAAAAAGATGCGATGGTGCCAATGGGTGGCTTACTCTGCTTTAAAGATAAATCCATGGAAGACGTGTATAACGAGT
GTCGTACACTTTGTGTCGTACAAGAAGGCTTCCCGACCTATGGCGGTTTAGAAGGGGGCGCAATGGAGCGTTTAGCGGTG
GGGCTACGTGATGGTATGCGTCAAGATTGGTTAGCGTATCGTATTAATCAAATTGAGTATTTGGTGAATGGTTTAGAAGC
GATTGGCGTCGTATGTCAACAACCTGGTGGTCACGCGGCATTTGTTGACGCAGGAAAATTGTTACCACATATTCCAGCCG
ATCAATTCCCTGCGCAAGCCCTTGCTTGTGAACTTTATAAGGTGGCGGGAATTCGTGCAGTAGAAATCGGTTCGTTCTTA
TTAGGTCGTGATCCAAAAACCGGTAAACAATTGCCTTGTCCTGCGGAGTTATTACGTTTAACGATTCCTCGTGCAACTTA
TACTCAAACGCACATGGACTTCATTATTGAAGCATTTAAGCAAGTCAAAGAAAATGCAGAAAATATTAAAGGGTTAACCT
TTACTTATGAGCCAAAAGTTTTACGTCACTTCACGGCACGCTTAAAAGAAGTATAA

Upstream 100 bases:

>100_bases
CGCTAATCCTTTACGTTTCAAACTCGAGATATTTTTTTATTTGGAATAAATCCTGATTTATTTCTTCCTCTTCTTTTAGC
TTACTTTTAAGGATTTTATT

Downstream 100 bases:

>100_bases
CAAGCTTAATGCAGTTTCTGAGCGATCAGGAACTGCATTTTTTATTGATTTTATTTTGAAAGGAAATGCATTATGAAAAA
GACGCCTTCAATTTTCGGTG

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MENFKHLPEPFRIRVIEPVRRTTRSYREEAILKAGMNPFLLDSEDVFIDLLTDSGTGAVTQEMQAAMLRGDEAYSGSRSY
HALANAVKEIFGYEYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVVFSNYFFDTTQGHSQINGATVRNVYTKEAFD
TSVNADFKGDFDLEKLEQGIQEVGAENVPYIVCTITCNSAGGQPVSLANMRAMYEIAKKYDIPVVMDSARFAENAYFIQQ
REPGYKEWTIEQITYESYKYADALAMSAKKDAMVPMGGLLCFKDKSMEDVYNECRTLCVVQEGFPTYGGLEGGAMERLAV
GLRDGMRQDWLAYRINQIEYLVNGLEAIGVVCQQPGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKQVKENAENIKGLTFTYEPKVLRHFTARLKEV

Sequences:

>Translated_471_residues
MENFKHLPEPFRIRVIEPVRRTTRSYREEAILKAGMNPFLLDSEDVFIDLLTDSGTGAVTQEMQAAMLRGDEAYSGSRSY
HALANAVKEIFGYEYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVVFSNYFFDTTQGHSQINGATVRNVYTKEAFD
TSVNADFKGDFDLEKLEQGIQEVGAENVPYIVCTITCNSAGGQPVSLANMRAMYEIAKKYDIPVVMDSARFAENAYFIQQ
REPGYKEWTIEQITYESYKYADALAMSAKKDAMVPMGGLLCFKDKSMEDVYNECRTLCVVQEGFPTYGGLEGGAMERLAV
GLRDGMRQDWLAYRINQIEYLVNGLEAIGVVCQQPGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKQVKENAENIKGLTFTYEPKVLRHFTARLKEV
>Mature_471_residues
MENFKHLPEPFRIRVIEPVRRTTRSYREEAILKAGMNPFLLDSEDVFIDLLTDSGTGAVTQEMQAAMLRGDEAYSGSRSY
HALANAVKEIFGYEYTIPTHQGRGAEQIYIPVLIKKREQEKGLDRSKMVVFSNYFFDTTQGHSQINGATVRNVYTKEAFD
TSVNADFKGDFDLEKLEQGIQEVGAENVPYIVCTITCNSAGGQPVSLANMRAMYEIAKKYDIPVVMDSARFAENAYFIQQ
REPGYKEWTIEQITYESYKYADALAMSAKKDAMVPMGGLLCFKDKSMEDVYNECRTLCVVQEGFPTYGGLEGGAMERLAV
GLRDGMRQDWLAYRINQIEYLVNGLEAIGVVCQQPGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFL
LGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKQVKENAENIKGLTFTYEPKVLRHFTARLKEV

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family [H]

Homologues:

Organism=Escherichia coli, GI87082323, Length=471, Percent_Identity=88.9596602972399, Blast_Score=877, Evalue=0.0,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR013440
- InterPro:   IPR018176 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: =4.1.99.1 [H]

Molecular weight: Translated: 52894; Mature: 52894

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENFKHLPEPFRIRVIEPVRRTTRSYREEAILKAGMNPFLLDSEDVFIDLLTDSGTGAVT
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHH
QEMQAAMLRGDEAYSGSRSYHALANAVKEIFGYEYTIPTHQGRGAEQIYIPVLIKKREQE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEEEECCHHH
KGLDRSKMVVFSNYFFDTTQGHSQINGATVRNVYTKEAFDTSVNADFKGDFDLEKLEQGI
CCCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
QEVGAENVPYIVCTITCNSAGGQPVSLANMRAMYEIAKKYDIPVVMDSARFAENAYFIQQ
HHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCEEEEEE
REPGYKEWTIEQITYESYKYADALAMSAKKDAMVPMGGLLCFKDKSMEDVYNECRTLCVV
CCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEE
QEGFPTYGGLEGGAMERLAVGLRDGMRQDWLAYRINQIEYLVNGLEAIGVVCQQPGGHAA
ECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
FVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAELLRL
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCCCCHHHHHHE
TIPRATYTQTHMDFIIEAFKQVKENAENIKGLTFTYEPKVLRHFTARLKEV
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCC
>Mature Secondary Structure
MENFKHLPEPFRIRVIEPVRRTTRSYREEAILKAGMNPFLLDSEDVFIDLLTDSGTGAVT
CCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEEEEECCCCCHHH
QEMQAAMLRGDEAYSGSRSYHALANAVKEIFGYEYTIPTHQGRGAEQIYIPVLIKKREQE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEEEEEECCHHH
KGLDRSKMVVFSNYFFDTTQGHSQINGATVRNVYTKEAFDTSVNADFKGDFDLEKLEQGI
CCCCCCEEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
QEVGAENVPYIVCTITCNSAGGQPVSLANMRAMYEIAKKYDIPVVMDSARFAENAYFIQQ
HHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEECCHHHHCCEEEEEE
REPGYKEWTIEQITYESYKYADALAMSAKKDAMVPMGGLLCFKDKSMEDVYNECRTLCVV
CCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHHHHHHEEEE
QEGFPTYGGLEGGAMERLAVGLRDGMRQDWLAYRINQIEYLVNGLEAIGVVCQQPGGHAA
ECCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
FVDAGKLLPHIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAELLRL
EEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHCCCCCCCCCCCCHHHHHHE
TIPRATYTQTHMDFIIEAFKQVKENAENIKGLTFTYEPKVLRHFTARLKEV
ECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA