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The map label for this gene is tnaB [H]

Identifier: 15603284

GI number: 15603284

Start: 1601378

End: 1602604

Strand: Reverse

Name: tnaB [H]

Synonym: PM1419

Alternate gene names: 15603284

Gene position: 1602604-1601378 (Counterclockwise)

Preceding gene: 15603285

Following gene: 15603281

Centisome position: 70.99

GC content: 41.73

Gene sequence:

>1227_bases
ATGAAAAAGACGCCTTCAATTTTCGGTGGTGCCTGTATTATTGCCAGTGTGTGTGTCGGCGCGGGTATGTTAGGGTTACC
AAGCTCGGGTGCCGGTGCTTGGACAATTTGGTCTATTTTGACGATCTGTTTTACGATGTTTACCATGACAATTTCAGGTT
GGTTATTATTAGAAGCCTACAAGCATTATGATCTGCGTGCTTCGTTTAATACCGTGACCAAAAGCATGCTCGGGAATTCC
GTCAATACGATCAATAATCTTGCAGTTTATTTTGTCGGGGGGATTTTATTGTATGCTTATACTACCGCGTCTGGGGGTAT
CTTAAGTGGACTTGCGGCACCGTATTTTGCCTTAGATTCACGGATTTGGTCAGTGATTTTTGTCTTTGTTTTTTCCTTCT
TTGTTTGGCATTCAACGCGTATTGTTGACCGTGTTTCCGTTTTACTGATCATCTTTATGGCGCTTTCATTCCTTTTTAGT
GTCTTTGGTTTAACCGTAAATATTGATTTAAGCACTTTATTTGATACGAAAGGTACAGAATCACATTATGCGATGTATGC
AATGGGATTATTACCCGTGGCATTAACTTCCTTTGGCTATCATCATTCAGTTGCAACAATGCGAGCTTACTATGGTGATG
AGCGTAAAGCGAAATATGCGATCCTTGGCGGTACCGGTATCGCGTTAACTCTTTATCTATTATGGGTGGTATCGATTTTT
GGGAACTTACCACGCGATCAATTCGCACCAGTGTTAGCCAGTGACGGAAATTTAGATGTATTACTTGGCGCTTTGGGTAA
TGTGATTGAGTCTGCTTCAGTCAAACAAGCGATTAATGCGTTTTCAATTGCGGCGATTTTATCTTCATTTATTGGTGTGG
GATTAGGGGTGTTTGATTTCTTAGCAGATTTCTTTAAATTTGATAACAGCAAAGAAGGACGTGCGAAGTCTTGGGCGGTA
ACCTTCTTACCACCACTAATTCTTTCTGTCGCCTACCCACTCGGTTTCTTAAAAGCGATTGGTTATGCTGGCGCGGTAGC
CACTATTTGGACTTGTATTATCCCTGCCTTATTAGCAAGAAAATCACGTAGCTTACCAAATGGACAAACGGGCTTTATGG
TCGGTGGCGGCAATATTACGATTGTTGTGGTCATGGTATTTGGTATTGTGACTGCGTTATTCCACTTCCTTGCCATGTTT
GGCATGTTACCTGCATTTAAAGGGTAA

Upstream 100 bases:

>100_bases
CTTCACGGCACGCTTAAAAGAAGTATAACAAGCTTAATGCAGTTTCTGAGCGATCAGGAACTGCATTTTTTATTGATTTT
ATTTTGAAAGGAAATGCATT

Downstream 100 bases:

>100_bases
TTTGATAAAGATACTAAAAGGGGCGAGTAACGCCCCTTTTTGTTGATAAAGTGCGGTGTATTTTCAAAACATTTCAAAAA
ATGACCGCACTTTTGTCTAT

Product: TnaB

Products: Indole [Cytoplasm]; L-tryptophan [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Tryptophan permease [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKKTPSIFGGACIIASVCVGAGMLGLPSSGAGAWTIWSILTICFTMFTMTISGWLLLEAYKHYDLRASFNTVTKSMLGNS
VNTINNLAVYFVGGILLYAYTTASGGILSGLAAPYFALDSRIWSVIFVFVFSFFVWHSTRIVDRVSVLLIIFMALSFLFS
VFGLTVNIDLSTLFDTKGTESHYAMYAMGLLPVALTSFGYHHSVATMRAYYGDERKAKYAILGGTGIALTLYLLWVVSIF
GNLPRDQFAPVLASDGNLDVLLGALGNVIESASVKQAINAFSIAAILSSFIGVGLGVFDFLADFFKFDNSKEGRAKSWAV
TFLPPLILSVAYPLGFLKAIGYAGAVATIWTCIIPALLARKSRSLPNGQTGFMVGGGNITIVVVMVFGIVTALFHFLAMF
GMLPAFKG

Sequences:

>Translated_408_residues
MKKTPSIFGGACIIASVCVGAGMLGLPSSGAGAWTIWSILTICFTMFTMTISGWLLLEAYKHYDLRASFNTVTKSMLGNS
VNTINNLAVYFVGGILLYAYTTASGGILSGLAAPYFALDSRIWSVIFVFVFSFFVWHSTRIVDRVSVLLIIFMALSFLFS
VFGLTVNIDLSTLFDTKGTESHYAMYAMGLLPVALTSFGYHHSVATMRAYYGDERKAKYAILGGTGIALTLYLLWVVSIF
GNLPRDQFAPVLASDGNLDVLLGALGNVIESASVKQAINAFSIAAILSSFIGVGLGVFDFLADFFKFDNSKEGRAKSWAV
TFLPPLILSVAYPLGFLKAIGYAGAVATIWTCIIPALLARKSRSLPNGQTGFMVGGGNITIVVVMVFGIVTALFHFLAMF
GMLPAFKG
>Mature_408_residues
MKKTPSIFGGACIIASVCVGAGMLGLPSSGAGAWTIWSILTICFTMFTMTISGWLLLEAYKHYDLRASFNTVTKSMLGNS
VNTINNLAVYFVGGILLYAYTTASGGILSGLAAPYFALDSRIWSVIFVFVFSFFVWHSTRIVDRVSVLLIIFMALSFLFS
VFGLTVNIDLSTLFDTKGTESHYAMYAMGLLPVALTSFGYHHSVATMRAYYGDERKAKYAILGGTGIALTLYLLWVVSIF
GNLPRDQFAPVLASDGNLDVLLGALGNVIESASVKQAINAFSIAAILSSFIGVGLGVFDFLADFFKFDNSKEGRAKSWAV
TFLPPLILSVAYPLGFLKAIGYAGAVATIWTCIIPALLARKSRSLPNGQTGFMVGGGNITIVVVMVFGIVTALFHFLAMF
GMLPAFKG

Specific function: Involved in transporting tryptophan across the cytoplasmic membrane [H]

COG id: COG0814

COG function: function code E; Amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid/polyamine transporter 2 family. Mtr/TnaB/TyrP permease subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789552, Length=408, Percent_Identity=40.6862745098039, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1790145, Length=412, Percent_Identity=37.8640776699029, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788218, Length=404, Percent_Identity=32.4257425742574, Blast_Score=178, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002091
- InterPro:   IPR013061
- InterPro:   IPR013059
- InterPro:   IPR018227 [H]

Pfam domain/function: PF03222 Trp_Tyr_perm [H]

EC number: NA

Molecular weight: Translated: 43838; Mature: 43838

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKTPSIFGGACIIASVCVGAGMLGLPSSGAGAWTIWSILTICFTMFTMTISGWLLLEAY
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHYDLRASFNTVTKSMLGNSVNTINNLAVYFVGGILLYAYTTASGGILSGLAAPYFALDS
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RIWSVIFVFVFSFFVWHSTRIVDRVSVLLIIFMALSFLFSVFGLTVNIDLSTLFDTKGTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCC
SHYAMYAMGLLPVALTSFGYHHSVATMRAYYGDERKAKYAILGGTGIALTLYLLWVVSIF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHH
GNLPRDQFAPVLASDGNLDVLLGALGNVIESASVKQAINAFSIAAILSSFIGVGLGVFDF
CCCCHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADFFKFDNSKEGRAKSWAVTFLPPLILSVAYPLGFLKAIGYAGAVATIWTCIIPALLAR
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSRSLPNGQTGFMVGGGNITIVVVMVFGIVTALFHFLAMFGMLPAFKG
HHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKTPSIFGGACIIASVCVGAGMLGLPSSGAGAWTIWSILTICFTMFTMTISGWLLLEAY
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KHYDLRASFNTVTKSMLGNSVNTINNLAVYFVGGILLYAYTTASGGILSGLAAPYFALDS
HHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
RIWSVIFVFVFSFFVWHSTRIVDRVSVLLIIFMALSFLFSVFGLTVNIDLSTLFDTKGTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHHHCCCCCC
SHYAMYAMGLLPVALTSFGYHHSVATMRAYYGDERKAKYAILGGTGIALTLYLLWVVSIF
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHH
GNLPRDQFAPVLASDGNLDVLLGALGNVIESASVKQAINAFSIAAILSSFIGVGLGVFDF
CCCCHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LADFFKFDNSKEGRAKSWAVTFLPPLILSVAYPLGFLKAIGYAGAVATIWTCIIPALLAR
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSRSLPNGQTGFMVGGGNITIVVVMVFGIVTALFHFLAMFGMLPAFKG
HHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Indole [Periplasm]; L-tryptophan [Periplasm]; Proton [Periplasm] [C]

Specific reaction: Proton [Periplasm] + indole [Periplasm] = Proton [Cytoplasm] + indole [Cytoplasm] L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]