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The map label for this gene is dusA

Identifier: 15603283

GI number: 15603283

Start: 1600274

End: 1601257

Strand: Direct

Name: dusA

Synonym: PM1418

Alternate gene names: 15603283

Gene position: 1600274-1601257 (Clockwise)

Preceding gene: 15603282

Following gene: 15603286

Centisome position: 70.89

GC content: 46.14

Gene sequence:

>984_bases
ATGATCCAAAACCAACCGCACTTTTACCGTGGGCGCTTTTCCGTTGCCCCAATGCTCGATTGGACCACTCGCCATTGTCG
CTATTTCCATCGTCAATTTAGCCAACACGCCTTGCTCTATACCGAAATGGTGACAACAGGGGCGATTATTCATGCTAAGT
ATGACCACCTTGAATTTTCGCCAGCAGAAAACCCCGTAGCATTACAATTAGGCGGAAGCGATCCTACCCAACTCGCCCAA
TGTGCAAAAATCGCACAACAGCGAGGCTATACAGAAATCAATCTCAATGTTGGTTGCCCTTCTGATCGGGTGCAAAATGG
CATGTTCGGTGCCTGTTTAATGGCAAAAGCCGATTTAGTCGCCGACTGTGTCAGTGCGATGCAAACGGAAGTCAGCATTC
CCGTCACGGTAAAAACTCGCATTGGTATTGATGATCTCGACAGCTATGAATTCCTGTGTGAGTTTGTGCAGAAAGTACAT
GAAGCTGGTTGCCAAGAATTTATCATTCACGCCCGCAAAGCGTGGCTTTCTGGCTTAAGCCCGAAAGAAAACCGCGAAAT
TCCCCCCTTAGATTATGAACGCGTGTATCAACTCAAACGAGATTTCCCACACTTACTCATGAGCATTAATGGTGGCATTA
AAACCCTTGAAGAGATGCAACAACATTTGCAGTATATGGACGGCGTCATGGTGGGGCGTGAAGCCTATCAAAATCCGAGC
TTATTGGGCTATATTGATCAAGCCTTATTTGATCCAACCTGTCCTGTGGTTACCCCAAGAGAAGCTGTAGAAAAAATGTT
TCCTTATATCGAACAACAACTGAGCCAAGGCGTGTATTTAAATCATGTGGTTCGCCATATGCTTGGTGCATTCCAAAGCT
GTAAAGGGGCACGCCAATGGCGCCGTTATTTAAGTGAAAATGCGCACAAAGCCGGAGCTGGCTTGGAAGTGGTCGAAAAA
GCGTTAAGTTTTGTAGCAAGTTAA

Upstream 100 bases:

>100_bases
CGTCCATTATTTCACGCCAATTTCACCCAAAACCCTTTTATCATTTTGCGGCTGGACGTTTTCGCCAACGCATTCAAGAA
GACACAAGAGAACAAGAAAA

Downstream 100 bases:

>100_bases
CACTAAAAAACAAGCATAAAATAGACAAAAGTGCGGTCATTTTTTGAAATGTTTTGAAAATACACCGCACTTTATCAACA
AAAAGGGGCGTTACTCGCCC

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MIQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSQHALLYTEMVTTGAIIHAKYDHLEFSPAENPVALQLGGSDPTQLAQ
CAKIAQQRGYTEINLNVGCPSDRVQNGMFGACLMAKADLVADCVSAMQTEVSIPVTVKTRIGIDDLDSYEFLCEFVQKVH
EAGCQEFIIHARKAWLSGLSPKENREIPPLDYERVYQLKRDFPHLLMSINGGIKTLEEMQQHLQYMDGVMVGREAYQNPS
LLGYIDQALFDPTCPVVTPREAVEKMFPYIEQQLSQGVYLNHVVRHMLGAFQSCKGARQWRRYLSENAHKAGAGLEVVEK
ALSFVAS

Sequences:

>Translated_327_residues
MIQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSQHALLYTEMVTTGAIIHAKYDHLEFSPAENPVALQLGGSDPTQLAQ
CAKIAQQRGYTEINLNVGCPSDRVQNGMFGACLMAKADLVADCVSAMQTEVSIPVTVKTRIGIDDLDSYEFLCEFVQKVH
EAGCQEFIIHARKAWLSGLSPKENREIPPLDYERVYQLKRDFPHLLMSINGGIKTLEEMQQHLQYMDGVMVGREAYQNPS
LLGYIDQALFDPTCPVVTPREAVEKMFPYIEQQLSQGVYLNHVVRHMLGAFQSCKGARQWRRYLSENAHKAGAGLEVVEK
ALSFVAS
>Mature_327_residues
MIQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSQHALLYTEMVTTGAIIHAKYDHLEFSPAENPVALQLGGSDPTQLAQ
CAKIAQQRGYTEINLNVGCPSDRVQNGMFGACLMAKADLVADCVSAMQTEVSIPVTVKTRIGIDDLDSYEFLCEFVQKVH
EAGCQEFIIHARKAWLSGLSPKENREIPPLDYERVYQLKRDFPHLLMSINGGIKTLEEMQQHLQYMDGVMVGREAYQNPS
LLGYIDQALFDPTCPVVTPREAVEKMFPYIEQQLSQGVYLNHVVRHMLGAFQSCKGARQWRRYLSENAHKAGAGLEVVEK
ALSFVAS

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily

Homologues:

Organism=Homo sapiens, GI31742496, Length=248, Percent_Identity=28.6290322580645, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI40807366, Length=253, Percent_Identity=26.8774703557312, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI145693211, Length=320, Percent_Identity=72.5, Blast_Score=489, Evalue=1e-140,
Organism=Escherichia coli, GI1788462, Length=256, Percent_Identity=27.34375, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1789660, Length=256, Percent_Identity=25.390625, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=233, Percent_Identity=27.4678111587983, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=271, Percent_Identity=24.3542435424354, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24580595, Length=250, Percent_Identity=26, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19920448, Length=250, Percent_Identity=26, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUSA_PASMU (Q9CL29)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246357.1
- ProteinModelPortal:   Q9CL29
- SMR:   Q9CL29
- GeneID:   1244765
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1418
- NMPDR:   fig|272843.1.peg.1419
- HOGENOM:   HBG630545
- OMA:   RIGIDEQ
- ProtClustDB:   PRK11815
- BioCyc:   PMUL272843:PM1418-MONOMER
- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11082
- PIRSF:   PIRSF006621
- TIGRFAMs:   TIGR00742

Pfam domain/function: PF01207 Dus

EC number: 1.-.-.-

Molecular weight: Translated: 37048; Mature: 37048

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSQHALLYTEMVTTGAIIHAKYDHLEFS
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCC
PAENPVALQLGGSDPTQLAQCAKIAQQRGYTEINLNVGCPSDRVQNGMFGACLMAKADLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHH
ADCVSAMQTEVSIPVTVKTRIGIDDLDSYEFLCEFVQKVHEAGCQEFIIHARKAWLSGLS
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKENREIPPLDYERVYQLKRDFPHLLMSINGGIKTLEEMQQHLQYMDGVMVGREAYQNPS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LLGYIDQALFDPTCPVVTPREAVEKMFPYIEQQLSQGVYLNHVVRHMLGAFQSCKGARQW
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RRYLSENAHKAGAGLEVVEKALSFVAS
HHHHHCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIQNQPHFYRGRFSVAPMLDWTTRHCRYFHRQFSQHALLYTEMVTTGAIIHAKYDHLEFS
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEECCCCCCC
PAENPVALQLGGSDPTQLAQCAKIAQQRGYTEINLNVGCPSDRVQNGMFGACLMAKADLV
CCCCCEEEEECCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHCCCHHHHHHHHHHHH
ADCVSAMQTEVSIPVTVKTRIGIDDLDSYEFLCEFVQKVHEAGCQEFIIHARKAWLSGLS
HHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PKENREIPPLDYERVYQLKRDFPHLLMSINGGIKTLEEMQQHLQYMDGVMVGREAYQNPS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LLGYIDQALFDPTCPVVTPREAVEKMFPYIEQQLSQGVYLNHVVRHMLGAFQSCKGARQW
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RRYLSENAHKAGAGLEVVEKALSFVAS
HHHHHCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100