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The map label for this gene is nadR [H]

Identifier: 15603252

GI number: 15603252

Start: 1582621

End: 1583907

Strand: Direct

Name: nadR [H]

Synonym: PM1387

Alternate gene names: 15603252

Gene position: 1582621-1583907 (Clockwise)

Preceding gene: 15603249

Following gene: 15603253

Centisome position: 70.11

GC content: 36.91

Gene sequence:

>1287_bases
ATGTCCACGTTTGCCTACTTACAACAAAAGCGTAAACAACTGAATTTAAAAGTTAATGAGATCTGTGCACAAGCCAATAT
TACACGTGCGTATTTTAATCAACTCGTGAGTGGAAAAATCAAAAATCCAAGCGCAACAAAGCTCAGTGCATTACATAAAG
TGTTGCAGATCACCGAACCGAATAATAAGAAAGTCGGTGTGATTTTTGGCAAATTTTATCCTGTCCATACCGGTCATATT
AATATGATCTATGAAGCCTTTAGTAAAGTGGATGAAATACACGTCATTGTCTGTAGTGATACAGAACGTGACTTAAAACT
CTTTTATGACAGCAAAATGAAACGTATGCCGACAGTGCAAGATCGCTTACGTTGGATGCAACAGATTTTTAAATACCAAA
AAAACCAGATTTTTATTCATCATCTTGTTGAAGACGGAATTCCAAGCTACCCAAATGGTTGGGAAGCGTGGGCGTTGCAA
GTCAAGAACCTCTTTAAAGAAAAACATTTCAATCCAACCGTAGTTTTCAGTAGCGAAGTGCAAGATAAAGCACCTTATGA
AAAATACCTTGGTTTAGAAGTTTCCTTAGTCGATCCTGAGCGACAATTTTTTAATGTCTCTGCGACAAAAATCCGCAATA
ACCCATTTCACTATTGGAAATTTATCCCAAAAGAAGTACGCCCGTTCTTTGCCAAAACTATCGCTATTTTAGGAGGGGAA
AGCAGTGGTAAAACTGTACTGGTCAATAAATTAGCCACAGTATTCAATACCACGTCTGCCTGGGAATATGGACGCGAGTT
TGTTTTCGAGAAATTAGGGGGAGATGAACAAGCGATGCAATATTCTGATTACCCACAAATGGCACTGGGGCACCAACGCT
ATATTGATTATGCGGTACGTCATGCACATAAAGTGGCGATTATTGATACAGATTTCATCACCACACAAGCTTTTTGTATT
CAATACGAAGGAAAAGCGCATCCTTTCTTAGATTCCATGATCAAAGAATATCCATTCGATGTCACAATTTTATTAAATAA
CAACACTAAATGGGTGGATGATGGCTTACGTAGTTTAGGTAATCATAAACAACGCCAACGTTTTCAACTCTTACTAAAAA
AATTACTCGATAAATACAATGTACCTTATATTGAAATTGAATCGCCAAGTTATTTAGAACGTTATAATCAGGTCAAACAG
ATTGTCGAGAAAATCCTAAATGAAGAAGAAATTCCTAATTTAATGCATGATCATGGGACAACATCCATTTTTGAGGAAGA
AGAATGA

Upstream 100 bases:

>100_bases
GTTGATGAATTACTTGCACAACAAAACCAAGCATAACCTTGCGCCACTCGCTGAAAAGTGCGGTCTTCACTTTACTATTT
CTGAATAAAAGGAAAGCATC

Downstream 100 bases:

>100_bases
TTTTATTTGCTGGTGATCCGCATGGAAGTTTTGATCACCTTTATCCTTTCATCAAAGATAAACAGGATGTTGCTTTAATT
ATTTTAGGGGACTTACAACT

Product: nicotinamide-nucleotide adenylyltransferase

Products: NA

Alternate protein names: Nicotinamide mononucleotide adenylyltransferase; NMN adenylyltransferase; NMN-AT; NMNAT; Nicotinamide ribonucleotide adenylyltransferase; Nicotinamide-nucleotide adenylyltransferase; Ribosylnicotinamide kinase; RNK; Nicotinamide riboside kinase; NRK; NmR-K [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSTFAYLQQKRKQLNLKVNEICAQANITRAYFNQLVSGKIKNPSATKLSALHKVLQITEPNNKKVGVIFGKFYPVHTGHI
NMIYEAFSKVDEIHVIVCSDTERDLKLFYDSKMKRMPTVQDRLRWMQQIFKYQKNQIFIHHLVEDGIPSYPNGWEAWALQ
VKNLFKEKHFNPTVVFSSEVQDKAPYEKYLGLEVSLVDPERQFFNVSATKIRNNPFHYWKFIPKEVRPFFAKTIAILGGE
SSGKTVLVNKLATVFNTTSAWEYGREFVFEKLGGDEQAMQYSDYPQMALGHQRYIDYAVRHAHKVAIIDTDFITTQAFCI
QYEGKAHPFLDSMIKEYPFDVTILLNNNTKWVDDGLRSLGNHKQRQRFQLLLKKLLDKYNVPYIEIESPSYLERYNQVKQ
IVEKILNEEEIPNLMHDHGTTSIFEEEE

Sequences:

>Translated_428_residues
MSTFAYLQQKRKQLNLKVNEICAQANITRAYFNQLVSGKIKNPSATKLSALHKVLQITEPNNKKVGVIFGKFYPVHTGHI
NMIYEAFSKVDEIHVIVCSDTERDLKLFYDSKMKRMPTVQDRLRWMQQIFKYQKNQIFIHHLVEDGIPSYPNGWEAWALQ
VKNLFKEKHFNPTVVFSSEVQDKAPYEKYLGLEVSLVDPERQFFNVSATKIRNNPFHYWKFIPKEVRPFFAKTIAILGGE
SSGKTVLVNKLATVFNTTSAWEYGREFVFEKLGGDEQAMQYSDYPQMALGHQRYIDYAVRHAHKVAIIDTDFITTQAFCI
QYEGKAHPFLDSMIKEYPFDVTILLNNNTKWVDDGLRSLGNHKQRQRFQLLLKKLLDKYNVPYIEIESPSYLERYNQVKQ
IVEKILNEEEIPNLMHDHGTTSIFEEEE
>Mature_427_residues
STFAYLQQKRKQLNLKVNEICAQANITRAYFNQLVSGKIKNPSATKLSALHKVLQITEPNNKKVGVIFGKFYPVHTGHIN
MIYEAFSKVDEIHVIVCSDTERDLKLFYDSKMKRMPTVQDRLRWMQQIFKYQKNQIFIHHLVEDGIPSYPNGWEAWALQV
KNLFKEKHFNPTVVFSSEVQDKAPYEKYLGLEVSLVDPERQFFNVSATKIRNNPFHYWKFIPKEVRPFFAKTIAILGGES
SGKTVLVNKLATVFNTTSAWEYGREFVFEKLGGDEQAMQYSDYPQMALGHQRYIDYAVRHAHKVAIIDTDFITTQAFCIQ
YEGKAHPFLDSMIKEYPFDVTILLNNNTKWVDDGLRSLGNHKQRQRFQLLLKKLLDKYNVPYIEIESPSYLERYNQVKQI
VEKILNEEEIPNLMHDHGTTSIFEEEE

Specific function: This enzyme has two activities:nicotinamide mononucleotide (NMN) adenylyltransferase and ribosylnicotinamide (RN) kinase. The RN kinase activity catalyzes the phosphorylation of RN to form nicotinamide ribonucleotide. The NMN adenylyltransferase activity

COG id: COG3172

COG function: function code H; Predicted ATPase/kinase involved in NAD metabolism

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein. Cytoplasm. Note=Found as a soluble cytoplasmic protein as well as a membrane-associated protein. In combination with corepressor (NAD), the cytoplasmic form of NadR would be capable of acting as a transcriptiona

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the bacterial RNK family [H]

Homologues:

Organism=Escherichia coli, GI87082440, Length=410, Percent_Identity=52.4390243902439, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016429
- InterPro:   IPR004821
- InterPro:   IPR006417
- InterPro:   IPR014729 [H]

Pfam domain/function: NA

EC number: =2.7.7.1; =2.7.1.22 [H]

Molecular weight: Translated: 50058; Mature: 49927

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFAYLQQKRKQLNLKVNEICAQANITRAYFNQLVSGKIKNPSATKLSALHKVLQITEP
CCHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
NNKKVGVIFGKFYPVHTGHINMIYEAFSKVDEIHVIVCSDTERDLKLFYDSKMKRMPTVQ
CCCEEEEEEEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHH
DRLRWMQQIFKYQKNQIFIHHLVEDGIPSYPNGWEAWALQVKNLFKEKHFNPTVVFSSEV
HHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCC
QDKAPYEKYLGLEVSLVDPERQFFNVSATKIRNNPFHYWKFIPKEVRPFFAKTIAILGGE
CCCCCHHHHCCCEEEEECCHHHHEECCHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCC
SSGKTVLVNKLATVFNTTSAWEYGREFVFEKLGGDEQAMQYSDYPQMALGHQRYIDYAVR
CCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHH
HAHKVAIIDTDFITTQAFCIQYEGKAHPFLDSMIKEYPFDVTILLNNNTKWVDDGLRSLG
HHCEEEEEECCHHEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHC
NHKQRQRFQLLLKKLLDKYNVPYIEIESPSYLERYNQVKQIVEKILNEEEIPNLMHDHGT
CHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCHHCCCHHHHCCCC
TSIFEEEE
CCCCCCCC
>Mature Secondary Structure 
STFAYLQQKRKQLNLKVNEICAQANITRAYFNQLVSGKIKNPSATKLSALHKVLQITEP
CHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
NNKKVGVIFGKFYPVHTGHINMIYEAFSKVDEIHVIVCSDTERDLKLFYDSKMKRMPTVQ
CCCEEEEEEEEEEEEECCHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHCCCHH
DRLRWMQQIFKYQKNQIFIHHLVEDGIPSYPNGWEAWALQVKNLFKEKHFNPTVVFSSEV
HHHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCC
QDKAPYEKYLGLEVSLVDPERQFFNVSATKIRNNPFHYWKFIPKEVRPFFAKTIAILGGE
CCCCCHHHHCCCEEEEECCHHHHEECCHHHCCCCCCHHHHCCCHHHHHHHHHHHHHCCCC
SSGKTVLVNKLATVFNTTSAWEYGREFVFEKLGGDEQAMQYSDYPQMALGHQRYIDYAVR
CCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHH
HAHKVAIIDTDFITTQAFCIQYEGKAHPFLDSMIKEYPFDVTILLNNNTKWVDDGLRSLG
HHCEEEEEECCHHEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHC
NHKQRQRFQLLLKKLLDKYNVPYIEIESPSYLERYNQVKQIVEKILNEEEIPNLMHDHGT
CHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCHHCCCHHHHCCCC
TSIFEEEE
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800; 12446641 [H]