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The map label for this gene is potD_3 [H]

Identifier: 15603249

GI number: 15603249

Start: 1580293

End: 1581228

Strand: Direct

Name: potD_3 [H]

Synonym: PM1384

Alternate gene names: 15603249

Gene position: 1580293-1581228 (Clockwise)

Preceding gene: 15603245

Following gene: 15603252

Centisome position: 70.0

GC content: 36.43

Gene sequence:

>936_bases
ATTCCTTCAAGTTTAATTGCTGATTTTACTAAAGAAACGGACATTGAAGTCATCTATTCTACTTTTGAGAGTAATGAAGA
GATGTACGCGAAACTCAAATTACTTTCTAAAAATAGCGGTTATGACCTGGTTTTTCCATCGAGTTATTACATCAATAAAA
TGGTCAAAGATGGCATGCTACAAAAACTCGATCACGCAAAATTAACCAATTTACATCAAATCCCAACCCATTTGCTGAAT
AAAGAATTTGATCCACAAAACCAATATTCCCTGCCTTATATTTATGGACTCACTGGTATTGGTATTAATGCAGAAGAGAT
TGATCCTAGCAAAATTACTAGCTGGGCAGATTTATGGAAACCAGAATATAAAGGTAAAGTGTTACTAACTAGCGATGCAC
GCGAAGTATTTCATATTGCTTTACTGTTAAATGGCAAATCACCAAACACCACTAAAGAGCTTGAAATTAAACAAGCTTAT
GAAAAACTGGTTAAACTCATGCCAAACGTTTTGGTATTTAATTCTGATTCACCAGAGGTCCCTTATGTACAAGGTGAGGT
TGCTTTAGGCATGATTTGGAATGGTTCCGCCTACTTAGGTAATAAAGAAAATTCCAACATTCAGTTTATTTACCCAAAAG
AAGGGGCGATTTTCTGGATGGATAACTATGCTATCCCGAAAAAAGCAAAAAATGTTGAGGGCGCATATCAATTTATCGAC
TTCTTATTACGTCCTGAAAATGCCAAAATTGTGATCGAAAGAATGGGATTTTCAATGCCAAATGAAGGTGTAAAACACTT
ACTCGCGCCTGAAATGGTCAACAACCCGGTTCTTTTCCCACCAGCAGAGGAAGTGGAAAAAGGCATCATGCAAGGCGATG
TCGGTAGAGCAGTCGATATTTATGAAAAATATTGGAACAAGCTGAAAACGAATTAA

Upstream 100 bases:

>100_bases
TGCTAACGTCATCAAATCACTTGTCTTCACCTCCCTTTTCTTTTTCACACATTCCGCCTTAGCAAACAAAAAGCTTTACG
TTTACAACTGGACGGATTAC

Downstream 100 bases:

>100_bases
TTATCCTAATACAAAAAGTGCGGTCAGATAAAATTTTGACCGCACTTTTTTATACACACTAAATATTACACATCATACTT
TTGACGATAAGCCACTAAAT

Product: PotD

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MPSSLIADFTKETDIEVIYSTFESNEEMYAKLKLLSKNSGYDLVFPSSYYINKMVKDGMLQKLDHAKLTNLHQIPTHLLN
KEFDPQNQYSLPYIYGLTGIGINAEEIDPSKITSWADLWKPEYKGKVLLTSDAREVFHIALLLNGKSPNTTKELEIKQAY
EKLVKLMPNVLVFNSDSPEVPYVQGEVALGMIWNGSAYLGNKENSNIQFIYPKEGAIFWMDNYAIPKKAKNVEGAYQFID
FLLRPENAKIVIERMGFSMPNEGVKHLLAPEMVNNPVLFPPAEEVEKGIMQGDVGRAVDIYEKYWNKLKTN

Sequences:

>Translated_311_residues
MPSSLIADFTKETDIEVIYSTFESNEEMYAKLKLLSKNSGYDLVFPSSYYINKMVKDGMLQKLDHAKLTNLHQIPTHLLN
KEFDPQNQYSLPYIYGLTGIGINAEEIDPSKITSWADLWKPEYKGKVLLTSDAREVFHIALLLNGKSPNTTKELEIKQAY
EKLVKLMPNVLVFNSDSPEVPYVQGEVALGMIWNGSAYLGNKENSNIQFIYPKEGAIFWMDNYAIPKKAKNVEGAYQFID
FLLRPENAKIVIERMGFSMPNEGVKHLLAPEMVNNPVLFPPAEEVEKGIMQGDVGRAVDIYEKYWNKLKTN
>Mature_310_residues
PSSLIADFTKETDIEVIYSTFESNEEMYAKLKLLSKNSGYDLVFPSSYYINKMVKDGMLQKLDHAKLTNLHQIPTHLLNK
EFDPQNQYSLPYIYGLTGIGINAEEIDPSKITSWADLWKPEYKGKVLLTSDAREVFHIALLLNGKSPNTTKELEIKQAYE
KLVKLMPNVLVFNSDSPEVPYVQGEVALGMIWNGSAYLGNKENSNIQFIYPKEGAIFWMDNYAIPKKAKNVEGAYQFIDF
LLRPENAKIVIERMGFSMPNEGVKHLLAPEMVNNPVLFPPAEEVEKGIMQGDVGRAVDIYEKYWNKLKTN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=310, Percent_Identity=56.7741935483871, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1787078, Length=325, Percent_Identity=31.0769230769231, Blast_Score=161, Evalue=7e-41,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 35418; Mature: 35287

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSLIADFTKETDIEVIYSTFESNEEMYAKLKLLSKNSGYDLVFPSSYYINKMVKDGML
CCCHHHHHHCCCCCCEEEEEHHHCCHHHEEEEEEEECCCCCEEECCCHHHHHHHHHHHHH
QKLDHAKLTNLHQIPTHLLNKEFDPQNQYSLPYIYGLTGIGINAEEIDPSKITSWADLWK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHCCCHHHHHHHHHHCC
PEYKGKVLLTSDAREVFHIALLLNGKSPNTTKELEIKQAYEKLVKLMPNVLVFNSDSPEV
CCCCCEEEEECCCHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
PYVQGEVALGMIWNGSAYLGNKENSNIQFIYPKEGAIFWMDNYAIPKKAKNVEGAYQFID
CEEECCEEEEEEECCCEEECCCCCCCEEEEEECCCEEEEECCCCCCCHHCCCHHHHHHHH
FLLRPENAKIVIERMGFSMPNEGVKHLLAPEMVNNPVLFPPAEEVEKGIMQGDVGRAVDI
HHCCCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEECCCHHHHHHHHHCCCCCCHHHH
YEKYWNKLKTN
HHHHHHHHCCC
>Mature Secondary Structure 
PSSLIADFTKETDIEVIYSTFESNEEMYAKLKLLSKNSGYDLVFPSSYYINKMVKDGML
CCHHHHHHCCCCCCEEEEEHHHCCHHHEEEEEEEECCCCCEEECCCHHHHHHHHHHHHH
QKLDHAKLTNLHQIPTHLLNKEFDPQNQYSLPYIYGLTGIGINAEEIDPSKITSWADLWK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCHHCCCHHHHHHHHHHCC
PEYKGKVLLTSDAREVFHIALLLNGKSPNTTKELEIKQAYEKLVKLMPNVLVFNSDSPEV
CCCCCEEEEECCCHHEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
PYVQGEVALGMIWNGSAYLGNKENSNIQFIYPKEGAIFWMDNYAIPKKAKNVEGAYQFID
CEEECCEEEEEEECCCEEECCCCCCCEEEEEECCCEEEEECCCCCCCHHCCCHHHHHHHH
FLLRPENAKIVIERMGFSMPNEGVKHLLAPEMVNNPVLFPPAEEVEKGIMQGDVGRAVDI
HHCCCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEECCCHHHHHHHHHCCCCCCHHHH
YEKYWNKLKTN
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]