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The map label for this gene is alsB [H]

Identifier: 15603190

GI number: 15603190

Start: 1516243

End: 1517187

Strand: Reverse

Name: alsB [H]

Synonym: PM1325

Alternate gene names: 15603190

Gene position: 1517187-1516243 (Counterclockwise)

Preceding gene: 15603203

Following gene: 15603188

Centisome position: 67.21

GC content: 39.79

Gene sequence:

>945_bases
ATGATGATGTCAACATTTTTGAAGAAAACGATCACGGCAAGCGCCTTACTCATCGCCCTATCCGGTACTACGCTAGCTGC
AGAAAAACCTCAAATTGCCTTACTCATGAAAACATTAAGTAATGAATATTTCATTACAATGCGACAAGGAGCGGAAGAAA
AAGCAAAAGAGGTAAATATTGATTTAATTGTACAAGTTGCAGAAAAGGAAGATTCAACAGAACAGCTTGTGGGTCTAGTC
GAAAACATGATTGCCAAAAAAGTGGATGGCATCATTGTTACCCCAAATGATTCTATCGCCTTTATTCCAGCATTTCAAAA
AGCTGAAAAAGCCGGGATTCCGATTATCGATCTCGATGTTCGTTTAGATGAAAAAGCAACGAAGAAAGCTGGGTTAAAAT
TTAATTACGTTGGTGTCGATAACTTCCATGGTGGTTATTTGGCGGCTAAAAATCTAGCACAAGCCTTAAACGAAATGGGG
GATGTTGCTATTTTAGAAGGGATTCCTGGTGTGGATAATGGCGAACAACGTAAAGCAGGTGCGCTCAAAGCATTTTCTGA
ATCACCAAATATCAAAGTTGTCGCTTCTCAGTCTGCAAACTGGGAAACAGAACAAGCACTTAATGTCACAACCAATATTT
TAACCGCACATCCGAATATCAAAGGGATTTTTGCGGCGAACGATAATATGGCACTGGGTGCAGTCACCGCGATTGAAAAT
GCCGGATTAGCAGGGAAGGTCCTTGTTTCTGGTTACGACGGTATTCCCCTTGCGATTGAATACGTGAAAAAAGGTAAAAT
GCAAAATACCATCGACCAGCTCCCGAAAAAACAAGTTGCCGTGGCTATCGAAAGTATTTTGAAAAAATTAAACAAACAGG
AACTACCTGATTTTTATTACGTTGATCCGGTAGTCGTTGATAAACAGAAAGCACAAGAATATTAA

Upstream 100 bases:

>100_bases
ATAATTTAAACCCTGTCTAAAAAATGTGAATGACATCACAGTTTGATCATTTTAATCTGTCTATACTAAAGTTTCCTTAT
TTAACGCTGAGAGGAGAGAG

Downstream 100 bases:

>100_bases
ATAGCTTGTTCTACCAAAAACACCGCACTTTTCATCTATACTGAAAGTGCGGTTTTTGATTGCTTGAAATTAGTGTCCAA
TCCCGACACAATTCGTCGAT

Product: hypothetical protein

Products: ADP; phosphate; ribose [Cytoplasm]; D-allose [Cytoplasm] [C]

Alternate protein names: ALBP [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MMMSTFLKKTITASALLIALSGTTLAAEKPQIALLMKTLSNEYFITMRQGAEEKAKEVNIDLIVQVAEKEDSTEQLVGLV
ENMIAKKVDGIIVTPNDSIAFIPAFQKAEKAGIPIIDLDVRLDEKATKKAGLKFNYVGVDNFHGGYLAAKNLAQALNEMG
DVAILEGIPGVDNGEQRKAGALKAFSESPNIKVVASQSANWETEQALNVTTNILTAHPNIKGIFAANDNMALGAVTAIEN
AGLAGKVLVSGYDGIPLAIEYVKKGKMQNTIDQLPKKQVAVAIESILKKLNKQELPDFYYVDPVVVDKQKAQEY

Sequences:

>Translated_314_residues
MMMSTFLKKTITASALLIALSGTTLAAEKPQIALLMKTLSNEYFITMRQGAEEKAKEVNIDLIVQVAEKEDSTEQLVGLV
ENMIAKKVDGIIVTPNDSIAFIPAFQKAEKAGIPIIDLDVRLDEKATKKAGLKFNYVGVDNFHGGYLAAKNLAQALNEMG
DVAILEGIPGVDNGEQRKAGALKAFSESPNIKVVASQSANWETEQALNVTTNILTAHPNIKGIFAANDNMALGAVTAIEN
AGLAGKVLVSGYDGIPLAIEYVKKGKMQNTIDQLPKKQVAVAIESILKKLNKQELPDFYYVDPVVVDKQKAQEY
>Mature_314_residues
MMMSTFLKKTITASALLIALSGTTLAAEKPQIALLMKTLSNEYFITMRQGAEEKAKEVNIDLIVQVAEKEDSTEQLVGLV
ENMIAKKVDGIIVTPNDSIAFIPAFQKAEKAGIPIIDLDVRLDEKATKKAGLKFNYVGVDNFHGGYLAAKNLAQALNEMG
DVAILEGIPGVDNGEQRKAGALKAFSESPNIKVVASQSANWETEQALNVTTNILTAHPNIKGIFAANDNMALGAVTAIEN
AGLAGKVLVSGYDGIPLAIEYVKKGKMQNTIDQLPKKQVAVAIESILKKLNKQELPDFYYVDPVVVDKQKAQEY

Specific function: Part of the binding-protein-dependent transport system AlsBAC for D-allose [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790526, Length=301, Percent_Identity=36.5448504983389, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1790192, Length=303, Percent_Identity=33.6633663366337, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1790674, Length=220, Percent_Identity=29.0909090909091, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1789990, Length=250, Percent_Identity=29.6, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788898, Length=307, Percent_Identity=27.3615635179153, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788473, Length=281, Percent_Identity=25.9786476868327, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33907; Mature: 33907

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMMSTFLKKTITASALLIALSGTTLAAEKPQIALLMKTLSNEYFITMRQGAEEKAKEVNI
CCHHHHHHHHHHHHEEEEEEECCEEECCCCHHHHHHHHHCCCEEEEEECCCHHHHHHCCE
DLIVQVAEKEDSTEQLVGLVENMIAKKVDGIIVTPNDSIAFIPAFQKAEKAGIPIIDLDV
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCEEEEECHHHHHHCCCCEEEEEE
RLDEKATKKAGLKFNYVGVDNFHGGYLAAKNLAQALNEMGDVAILEGIPGVDNGEQRKAG
EECCHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHH
ALKAFSESPNIKVVASQSANWETEQALNVTTNILTAHPNIKGIFAANDNMALGAVTAIEN
HHHHHCCCCCEEEEECCCCCCCHHHHHEEEEEEEEECCCCEEEEEECCCCEEEEEEHHHC
AGLAGKVLVSGYDGIPLAIEYVKKGKMQNTIDQLPKKQVAVAIESILKKLNKQELPDFYY
CCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEE
VDPVVVDKQKAQEY
ECCEEECHHHHCCC
>Mature Secondary Structure
MMMSTFLKKTITASALLIALSGTTLAAEKPQIALLMKTLSNEYFITMRQGAEEKAKEVNI
CCHHHHHHHHHHHHEEEEEEECCEEECCCCHHHHHHHHHCCCEEEEEECCCHHHHHHCCE
DLIVQVAEKEDSTEQLVGLVENMIAKKVDGIIVTPNDSIAFIPAFQKAEKAGIPIIDLDV
EEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCCCCEEEEECHHHHHHCCCCEEEEEE
RLDEKATKKAGLKFNYVGVDNFHGGYLAAKNLAQALNEMGDVAILEGIPGVDNGEQRKAG
EECCHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHH
ALKAFSESPNIKVVASQSANWETEQALNVTTNILTAHPNIKGIFAANDNMALGAVTAIEN
HHHHHCCCCCEEEEECCCCCCCHHHHHEEEEEEEEECCCCEEEEEECCCCEEEEEEHHHC
AGLAGKVLVSGYDGIPLAIEYVKKGKMQNTIDQLPKKQVAVAIESILKKLNKQELPDFYY
CCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEE
VDPVVVDKQKAQEY
ECCEEECHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; ribose [Periplasm]; D-allose [Periplasm]; ATP [C]

Specific reaction: ATP + H2O + ribose [Periplasm] = ADP + phosphate + ribose [Cytoplasm] D-allose [Periplasm] + ATP + H2O = D-allose [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8576032; 9401019; 10064713 [H]