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The map label for this gene is pdxT

Identifier: 15603098

GI number: 15603098

Start: 1425206

End: 1425787

Strand: Direct

Name: pdxT

Synonym: PM1233

Alternate gene names: 15603098

Gene position: 1425206-1425787 (Clockwise)

Preceding gene: 15603097

Following gene: 15603099

Centisome position: 63.13

GC content: 44.85

Gene sequence:

>582_bases
ATGAAAGACTATTCACATTTACACATTGGCGTGTTAGCTCTGCAGGGAGCAGTAAGCGAACATTTGCGCCAAATTGAACA
ACTTGGTGCCAACGCCAGTGCAATCAAAACCGTCTCAGAATTGACCGCACTTGATGGTTTAGTGCTCCCGGGCGGTGAAA
GCACGACCATTGGCAGATTAATGCGTCAATATGGGTTTATTGAGGCAATTCAAGATGTTGCCAAACAAGGTAAAGGTATT
TTCGGCACCTGTGCCGGCATGATTTTACTCGCAAAGCAATTAGAAAATGATCCTACGGTGCATTTAGGTTTAATGGACAT
CTGTGTGCAACGCAACGCCTTTGGGCGACAAGTGGATAGCTTTCAAACCGCCCTTGAAATTGAAGGCTTTGCTACAACGT
TTCCTGCAGTTTTTATCCGTGCACCACATATTGCTCAAGTCAATCATGAAAAAGTGCAATGTCTAGCGACTTTTCAGGGG
CATGTTGTCCTCGCGAAACAACAAAATTTGTTGGCTTGTGCCTTTCACCCAGAACTGACGACAGATCTGCGCGTCATGCA
ACACTTTTTAGAAATGTGTTAG

Upstream 100 bases:

>100_bases
TGATCGCCCGTTTATCGGAAGAATTAGGCGAACCGATGAAAGGCATTGAGATTTCCCGCCTGCAACAACATGAAAGAATG
CAAGAGCGTGGCTGGTAAGT

Downstream 100 bases:

>100_bases
TCTGTTTGAGGATGTGAGCACTCGCACATCCTCTTTTAAAAAATCTCATAATTTGACCGCACTTTTTCATCTCATCTTGC
ATGTTTCACAACTTTAATAG

Product: hypothetical protein

Products: NA

Alternate protein names: Glutamine amidotransferase glutaminase subunit pdxT

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MKDYSHLHIGVLALQGAVSEHLRQIEQLGANASAIKTVSELTALDGLVLPGGESTTIGRLMRQYGFIEAIQDVAKQGKGI
FGTCAGMILLAKQLENDPTVHLGLMDICVQRNAFGRQVDSFQTALEIEGFATTFPAVFIRAPHIAQVNHEKVQCLATFQG
HVVLAKQQNLLACAFHPELTTDLRVMQHFLEMC

Sequences:

>Translated_193_residues
MKDYSHLHIGVLALQGAVSEHLRQIEQLGANASAIKTVSELTALDGLVLPGGESTTIGRLMRQYGFIEAIQDVAKQGKGI
FGTCAGMILLAKQLENDPTVHLGLMDICVQRNAFGRQVDSFQTALEIEGFATTFPAVFIRAPHIAQVNHEKVQCLATFQG
HVVLAKQQNLLACAFHPELTTDLRVMQHFLEMC
>Mature_193_residues
MKDYSHLHIGVLALQGAVSEHLRQIEQLGANASAIKTVSELTALDGLVLPGGESTTIGRLMRQYGFIEAIQDVAKQGKGI
FGTCAGMILLAKQLENDPTVHLGLMDICVQRNAFGRQVDSFQTALEIEGFATTFPAVFIRAPHIAQVNHEKVQCLATFQG
HVVLAKQQNLLACAFHPELTTDLRVMQHFLEMC

Specific function: Involved in the hydrolysis of glutamine to glutamate and ammonia. Channels an ammonia molecule to pdxS

COG id: COG0311

COG function: function code H; Predicted glutamine amidotransferase involved in pyridoxine biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine amidotransferase pdxT/SNO family

Homologues:

Organism=Saccharomyces cerevisiae, GI6323995, Length=208, Percent_Identity=37.9807692307692, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321048, Length=208, Percent_Identity=37.5, Blast_Score=117, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6323742, Length=204, Percent_Identity=37.2549019607843, Blast_Score=111, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXT_PASMU (Q9CLJ5)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_246170.1
- ProteinModelPortal:   Q9CLJ5
- SMR:   Q9CLJ5
- GeneID:   1244580
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM1233
- NMPDR:   fig|272843.1.peg.1233
- HOGENOM:   HBG292341
- OMA:   QGDVREH
- BioCyc:   PMUL272843:PM1233-MONOMER
- HAMAP:   MF_01615
- InterPro:   IPR002161
- InterPro:   IPR021196
- PIRSF:   PIRSF005639
- TIGRFAMs:   TIGR03800

Pfam domain/function: PF01174 SNO

EC number: NA

Molecular weight: Translated: 21095; Mature: 21095

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS01236 PDXT_SNO_1; PS51130 PDXT_SNO_2

Important sites: ACT_SITE 84-84 ACT_SITE 176-176 ACT_SITE 178-178 BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDYSHLHIGVLALQGAVSEHLRQIEQLGANASAIKTVSELTALDGLVLPGGESTTIGRL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCCCHHHHHH
MRQYGFIEAIQDVAKQGKGIFGTCAGMILLAKQLENDPTVHLGLMDICVQRNAFGRQVDS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHHHH
FQTALEIEGFATTFPAVFIRAPHIAQVNHEKVQCLATFQGHVVLAKQQNLLACAFHPELT
HHHHHHHCCHHHHHHHHHHCCCCHHHCCHHHHHHHEECCCCEEEEECCCEEEEEECCCCH
TDLRVMQHFLEMC
HHHHHHHHHHHHC
>Mature Secondary Structure
MKDYSHLHIGVLALQGAVSEHLRQIEQLGANASAIKTVSELTALDGLVLPGGESTTIGRL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEEECCCCCHHHHHH
MRQYGFIEAIQDVAKQGKGIFGTCAGMILLAKQLENDPTVHLGLMDICVQRNAFGRQVDS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCHHHHHHHH
FQTALEIEGFATTFPAVFIRAPHIAQVNHEKVQCLATFQGHVVLAKQQNLLACAFHPELT
HHHHHHHCCHHHHHHHHHHCCCCHHHCCHHHHHHHEECCCCEEEEECCCEEEEEECCCCH
TDLRVMQHFLEMC
HHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100