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The map label for this gene is trmE [H]

Identifier: 15603031

GI number: 15603031

Start: 1361084

End: 1362481

Strand: Direct

Name: trmE [H]

Synonym: PM1166

Alternate gene names: 15603031

Gene position: 1361084-1362481 (Clockwise)

Preceding gene: 15603030

Following gene: 15603035

Centisome position: 60.29

GC content: 42.85

Gene sequence:

>1398_bases
TTGTTTTATCAAAGTGCGGTCATTTTTAAGAGAGTTTTTATGAAAGAAACCATTGTTGCACAAGCTACTGCACCGGGACG
CGGTGGAATTGGGATTTTACGAGTGTCAGGTCCGAAAGCAGTTGAGGTTGCCCATACGGTATTAGGTAAATGCCCCAAAC
CACGTATGGCAGATTATTTGCCCTTTAAAGATAGTGATGGAAATGTGCTCGATCAGGGCATTGCGCTGTACTTCAAAGCC
CCTCACTCCTTTACAGGCGAAGATGTGTTGGAATTACAAGGACATGGCGGTCAGGTTGTACTCGATCTGCTATTAAAACG
CATTTTACAGTTAGAGGGGCTCCGTTTAGCCCGTCCCGGTGAATTTTCAGAACAAGCTTTCTTAAACGATAAATTAGATT
TGGCGCAAGCCGAAGCGATTGCTGATTTAATTGATGCCAGTTCAGAGCAAGCCGCTCGCTCAGCATTAAAATCCCTACAA
GGTGAATTTTCTAATAAAGTGAATCAGCTCGTTGATAGTGTGATTTACTTACGGACCTATGTGGAAGCGGCAATTGATTT
CCCAGATGAAGAAATTGATTTTCTAGCAGACGGTAAAATTGAAGCCCACCTGAATGATATCATTACGCAGCTAGATCATG
TCCGTTCAGAGGCGAAACAAGGTTCGATTTTGCGTGAAGGGATGAAAGTAGTGATTGCTGGGCGACCAAATGCAGGGAAA
TCGAGCCTTCTCAATGCACTAGCTGGGCGTGAAGCAGCAATTGTCACGGATATTGCAGGAACAACCCGTGATGTGCTACG
TGAACATATTCATATTGATGGTATGCCACTGCACATTATTGATACAGCCGGTCTGCGAGAGGCTACGGATGAAGTCGAGC
GTATTGGAATCGTACGTGCTTGGAGTGAAATCGAACAAGCTGATCGCATTTTGTTAATGTTAGATAGTACAGAAGCCGAT
AACCAAGATTTAGAAAAAGTGCGGTCAGAATTTTTAACCAAATTGCCAAGCAATATTCCCGTCACGATTGTACGTAATAA
AGCGGATTTAAGTGGCGAAAATGAAGGCATAGTGGAGCAAAATGGTTATACTGTCATTACTCTTTCCGCTAAAACGCAAC
AAGGGATTGCGCTATTGCGTGAACATCTCAAACAATCCATGGGTTATCAAACCAATATGGAAGGAGGATTCCTCGCTCGC
CGTCGCCATCTTGAAGCACTAGAACAAGCAGCAACGCATTTACAACAAGGTCATGTGCAATTAACGCAATTCTATGCTGG
CGAATTATTGGCAGAAGAATTACGTCGAGTTCAGAATCATTTAAGTGAAATTACTGGGCAATTTACTTCCGATGATTTAC
TCGGCAATATTTTCAGTTCTTTCTGTATTGGTAAATAA

Upstream 100 bases:

>100_bases
CAACAGCAATTGATTTATCGTGGCTTAGAGAAAAAAGGGTTACACTCTCGCCATAAATAATTCAGTTGAATGAACATAAA
AGGCATCAAGTTGGTGCCTT

Downstream 100 bases:

>100_bases
ACAATCCATCACAAAAAAGTGCGGTGATTTTTTAGAAATCAACCGCACTTTGTTTAATACCTGATTGTTTGTTTCAATTA
CATCACAAGCGATGTTTTAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MFYQSAVIFKRVFMKETIVAQATAPGRGGIGILRVSGPKAVEVAHTVLGKCPKPRMADYLPFKDSDGNVLDQGIALYFKA
PHSFTGEDVLELQGHGGQVVLDLLLKRILQLEGLRLARPGEFSEQAFLNDKLDLAQAEAIADLIDASSEQAARSALKSLQ
GEFSNKVNQLVDSVIYLRTYVEAAIDFPDEEIDFLADGKIEAHLNDIITQLDHVRSEAKQGSILREGMKVVIAGRPNAGK
SSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREATDEVERIGIVRAWSEIEQADRILLMLDSTEAD
NQDLEKVRSEFLTKLPSNIPVTIVRNKADLSGENEGIVEQNGYTVITLSAKTQQGIALLREHLKQSMGYQTNMEGGFLAR
RRHLEALEQAATHLQQGHVQLTQFYAGELLAEELRRVQNHLSEITGQFTSDDLLGNIFSSFCIGK

Sequences:

>Translated_465_residues
MFYQSAVIFKRVFMKETIVAQATAPGRGGIGILRVSGPKAVEVAHTVLGKCPKPRMADYLPFKDSDGNVLDQGIALYFKA
PHSFTGEDVLELQGHGGQVVLDLLLKRILQLEGLRLARPGEFSEQAFLNDKLDLAQAEAIADLIDASSEQAARSALKSLQ
GEFSNKVNQLVDSVIYLRTYVEAAIDFPDEEIDFLADGKIEAHLNDIITQLDHVRSEAKQGSILREGMKVVIAGRPNAGK
SSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREATDEVERIGIVRAWSEIEQADRILLMLDSTEAD
NQDLEKVRSEFLTKLPSNIPVTIVRNKADLSGENEGIVEQNGYTVITLSAKTQQGIALLREHLKQSMGYQTNMEGGFLAR
RRHLEALEQAATHLQQGHVQLTQFYAGELLAEELRRVQNHLSEITGQFTSDDLLGNIFSSFCIGK
>Mature_465_residues
MFYQSAVIFKRVFMKETIVAQATAPGRGGIGILRVSGPKAVEVAHTVLGKCPKPRMADYLPFKDSDGNVLDQGIALYFKA
PHSFTGEDVLELQGHGGQVVLDLLLKRILQLEGLRLARPGEFSEQAFLNDKLDLAQAEAIADLIDASSEQAARSALKSLQ
GEFSNKVNQLVDSVIYLRTYVEAAIDFPDEEIDFLADGKIEAHLNDIITQLDHVRSEAKQGSILREGMKVVIAGRPNAGK
SSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREATDEVERIGIVRAWSEIEQADRILLMLDSTEAD
NQDLEKVRSEFLTKLPSNIPVTIVRNKADLSGENEGIVEQNGYTVITLSAKTQQGIALLREHLKQSMGYQTNMEGGFLAR
RRHLEALEQAATHLQQGHVQLTQFYAGELLAEELRRVQNHLSEITGQFTSDDLLGNIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=465, Percent_Identity=35.2688172043011, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=33.6016096579477, Blast_Score=218, Evalue=1e-56,
Organism=Homo sapiens, GI193082993, Length=459, Percent_Identity=33.7690631808279, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI2367268, Length=451, Percent_Identity=77.3835920177384, Blast_Score=716, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=215, Percent_Identity=33.0232558139535, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17507259, Length=455, Percent_Identity=29.010989010989, Blast_Score=174, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=32.0486815415822, Blast_Score=223, Evalue=3e-59,
Organism=Drosophila melanogaster, GI45550813, Length=474, Percent_Identity=30.379746835443, Blast_Score=198, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 51155; Mature: 51155

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYQSAVIFKRVFMKETIVAQATAPGRGGIGILRVSGPKAVEVAHTVLGKCPKPRMADYL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCC
PFKDSDGNVLDQGIALYFKAPHSFTGEDVLELQGHGGQVVLDLLLKRILQLEGLRLARPG
CCCCCCCCCHHCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
EFSEQAFLNDKLDLAQAEAIADLIDASSEQAARSALKSLQGEFSNKVNQLVDSVIYLRTY
CCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEAAIDFPDEEIDFLADGKIEAHLNDIITQLDHVRSEAKQGSILREGMKVVIAGRPNAGK
HHHHHCCCCCHHCCEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCH
SSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREATDEVERIGIVRA
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
WSEIEQADRILLMLDSTEADNQDLEKVRSEFLTKLPSNIPVTIVRNKADLSGENEGIVEQ
HHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEC
NGYTVITLSAKTQQGIALLREHLKQSMGYQTNMEGGFLARRRHLEALEQAATHLQQGHVQ
CCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
LTQFYAGELLAEELRRVQNHLSEITGQFTSDDLLGNIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFYQSAVIFKRVFMKETIVAQATAPGRGGIGILRVSGPKAVEVAHTVLGKCPKPRMADYL
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCC
PFKDSDGNVLDQGIALYFKAPHSFTGEDVLELQGHGGQVVLDLLLKRILQLEGLRLARPG
CCCCCCCCCHHCCEEEEEECCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCC
EFSEQAFLNDKLDLAQAEAIADLIDASSEQAARSALKSLQGEFSNKVNQLVDSVIYLRTY
CCCHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VEAAIDFPDEEIDFLADGKIEAHLNDIITQLDHVRSEAKQGSILREGMKVVIAGRPNAGK
HHHHHCCCCCHHCCEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCH
SSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREATDEVERIGIVRA
HHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHH
WSEIEQADRILLMLDSTEADNQDLEKVRSEFLTKLPSNIPVTIVRNKADLSGENEGIVEQ
HHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEC
NGYTVITLSAKTQQGIALLREHLKQSMGYQTNMEGGFLARRRHLEALEQAATHLQQGHVQ
CCEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHH
LTQFYAGELLAEELRRVQNHLSEITGQFTSDDLLGNIFSSFCIGK
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]