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The map label for this gene is dusB [H]

Identifier: 15602952

GI number: 15602952

Start: 1282622

End: 1283626

Strand: Reverse

Name: dusB [H]

Synonym: PM1087

Alternate gene names: 15602952

Gene position: 1283626-1282622 (Counterclockwise)

Preceding gene: 15602953

Following gene: 15602951

Centisome position: 56.86

GC content: 39.2

Gene sequence:

>1005_bases
ATGACAGTGCGGATAGGTTCTTATCAGCTTAAAAATCGCATTTTTCTTGCTCCTATGGCTGGCATCACTGACCAACCATT
TCGGCGAATCTGCACTCATTATGGGGCAGGTTTAACTTTTTCTGAAATGATGTCAACGAATCCGCAAGTCTGGCATACCG
AAAAATCGAAACTGCGCTTGGCTCATCATCAAGAAGCAGGAATTAATGCTGTGCAAATAGCTGGTTGTGATCCCGATGAG
ATGGCGAAAGCTGCTCAAATCAATGTAGAATATGGGGCAGAAATTATTGATATCAATATGGGCTGCCCAGCCAAAAAAGT
GAATCGTAAAATGGCGGGCTCTGCGCTGTTACAATATCCTGATTTGGTCAAACAAATTCTTAATAAAGTTGTGAAATCTG
TTACTGTACCAGTGACATTAAAGATAAGAACAGGCTGGGATAAAGACAACCGAAATTGTTTAGAAATCGCTAAAATTGCA
GAGCAATGTGGTATTCAAGCACTGACCATCCACGGACGAACAAGGAGTTGTATGTTTGAGGGGGAGGCTGAATATGACAA
TATCAAGGCGGTCAAAGAGCAACTTTCTATTCCGATTATTGCCAATGGCGATATTACTTCCGCTGAAAAAGCAAAGTATG
TTCTTGATTATACCAACGCAGATGCAATAATGATCGGACGTGGTTCATTAGGCAATCCGTGGCTTTTCCGAGTTATGGAA
AGCTTAATTGAAAAAGACTCGATTGTTTTAGAGCCAAGTTTAAACGAGAAATGTAATGTGATTTTACAGCATATCCAAGA
ACTGCATCAATTTTATGGTGTGGAGAAAGGATGTCGTATTGCACGTAAACACGTTGCTTGGTATTTACAGGGAATCCAAC
CTAATCCCGTTTTTAGACAGGCTTTTAATGCAATTACTGATCCCAAAGAACAATTAATTGCTTTAGAAGGTTTTTTTAAT
TTGATTCTGATGGATAAAGAAAAAAATGTTAGAACAACAACGTAA

Upstream 100 bases:

>100_bases
AAATATGCTTTCCAAATGGCTTAAAAGTGCGTATTATAGCGTCCCTTATCGGTCCGGGTATGTTTAACCGATAAAATAGG
ATTTGGATGTTAAAGGAGTA

Downstream 100 bases:

>100_bases
TCCTGCTGATGCACTAACTGTATCAGTGTTAAATTCACAATCTCAAGTCACAAATAAACCATTGCGTGATTCTGTGAAAC
AAGCATTGAGAAATTATTTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTVRIGSYQLKNRIFLAPMAGITDQPFRRICTHYGAGLTFSEMMSTNPQVWHTEKSKLRLAHHQEAGINAVQIAGCDPDE
MAKAAQINVEYGAEIIDINMGCPAKKVNRKMAGSALLQYPDLVKQILNKVVKSVTVPVTLKIRTGWDKDNRNCLEIAKIA
EQCGIQALTIHGRTRSCMFEGEAEYDNIKAVKEQLSIPIIANGDITSAEKAKYVLDYTNADAIMIGRGSLGNPWLFRVME
SLIEKDSIVLEPSLNEKCNVILQHIQELHQFYGVEKGCRIARKHVAWYLQGIQPNPVFRQAFNAITDPKEQLIALEGFFN
LILMDKEKNVRTTT

Sequences:

>Translated_334_residues
MTVRIGSYQLKNRIFLAPMAGITDQPFRRICTHYGAGLTFSEMMSTNPQVWHTEKSKLRLAHHQEAGINAVQIAGCDPDE
MAKAAQINVEYGAEIIDINMGCPAKKVNRKMAGSALLQYPDLVKQILNKVVKSVTVPVTLKIRTGWDKDNRNCLEIAKIA
EQCGIQALTIHGRTRSCMFEGEAEYDNIKAVKEQLSIPIIANGDITSAEKAKYVLDYTNADAIMIGRGSLGNPWLFRVME
SLIEKDSIVLEPSLNEKCNVILQHIQELHQFYGVEKGCRIARKHVAWYLQGIQPNPVFRQAFNAITDPKEQLIALEGFFN
LILMDKEKNVRTTT
>Mature_333_residues
TVRIGSYQLKNRIFLAPMAGITDQPFRRICTHYGAGLTFSEMMSTNPQVWHTEKSKLRLAHHQEAGINAVQIAGCDPDEM
AKAAQINVEYGAEIIDINMGCPAKKVNRKMAGSALLQYPDLVKQILNKVVKSVTVPVTLKIRTGWDKDNRNCLEIAKIAE
QCGIQALTIHGRTRSCMFEGEAEYDNIKAVKEQLSIPIIANGDITSAEKAKYVLDYTNADAIMIGRGSLGNPWLFRVMES
LIEKDSIVLEPSLNEKCNVILQHIQELHQFYGVEKGCRIARKHVAWYLQGIQPNPVFRQAFNAITDPKEQLIALEGFFNL
ILMDKEKNVRTTT

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=239, Percent_Identity=33.0543933054393, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI239788462, Length=231, Percent_Identity=33.3333333333333, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI31742496, Length=223, Percent_Identity=33.1838565022422, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI8923374, Length=259, Percent_Identity=28.957528957529, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI40807366, Length=230, Percent_Identity=30.8695652173913, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=64.6687697160883, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1788462, Length=238, Percent_Identity=34.0336134453782, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI145693211, Length=248, Percent_Identity=27.0161290322581, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17543114, Length=234, Percent_Identity=35.4700854700855, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25144369, Length=213, Percent_Identity=32.3943661971831, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17510279, Length=249, Percent_Identity=31.7269076305221, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=225, Percent_Identity=31.1111111111111, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=295, Percent_Identity=27.4576271186441, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323560, Length=235, Percent_Identity=30.2127659574468, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323437, Length=227, Percent_Identity=28.6343612334802, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24585320, Length=268, Percent_Identity=29.8507462686567, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=32.15859030837, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=32.15859030837, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI19921524, Length=230, Percent_Identity=30, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI45549423, Length=259, Percent_Identity=26.6409266409266, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37489; Mature: 37358

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVRIGSYQLKNRIFLAPMAGITDQPFRRICTHYGAGLTFSEMMSTNPQVWHTEKSKLRL
CEEEECCEEECCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCEEECCHHHHHH
AHHQEAGINAVQIAGCDPDEMAKAAQINVEYGAEIIDINMGCPAKKVNRKMAGSALLQYP
HHHHCCCCCEEEEECCCHHHHHHHHEEEHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHH
DLVKQILNKVVKSVTVPVTLKIRTGWDKDNRNCLEIAKIAEQCGIQALTIHGRTRSCMFE
HHHHHHHHHHHHHEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEC
GEAEYDNIKAVKEQLSIPIIANGDITSAEKAKYVLDYTNADAIMIGRGSLGNPWLFRVME
CCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHH
SLIEKDSIVLEPSLNEKCNVILQHIQELHQFYGVEKGCRIARKHVAWYLQGIQPNPVFRQ
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHH
AFNAITDPKEQLIALEGFFNLILMDKEKNVRTTT
HHHHHCCCHHHHHHHHHHEEEEEEECCCCCCCCC
>Mature Secondary Structure 
TVRIGSYQLKNRIFLAPMAGITDQPFRRICTHYGAGLTFSEMMSTNPQVWHTEKSKLRL
EEEECCEEECCEEEEEECCCCCCCHHHHHHHHHCCCCCHHHHHCCCCCEEECCHHHHHH
AHHQEAGINAVQIAGCDPDEMAKAAQINVEYGAEIIDINMGCPAKKVNRKMAGSALLQYP
HHHHCCCCCEEEEECCCHHHHHHHHEEEHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHH
DLVKQILNKVVKSVTVPVTLKIRTGWDKDNRNCLEIAKIAEQCGIQALTIHGRTRSCMFE
HHHHHHHHHHHHHEECCEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCEEEEC
GEAEYDNIKAVKEQLSIPIIANGDITSAEKAKYVLDYTNADAIMIGRGSLGNPWLFRVME
CCCCHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEECCCCEEEEECCCCCCHHHHHHHH
SLIEKDSIVLEPSLNEKCNVILQHIQELHQFYGVEKGCRIARKHVAWYLQGIQPNPVFRQ
HHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHH
AFNAITDPKEQLIALEGFFNLILMDKEKNVRTTT
HHHHHCCCHHHHHHHHHHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]