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The map label for this gene is dat1 [H]

Identifier: 15602933

GI number: 15602933

Start: 1258461

End: 1259003

Strand: Reverse

Name: dat1 [H]

Synonym: PM1068

Alternate gene names: 15602933

Gene position: 1259003-1258461 (Counterclockwise)

Preceding gene: 15602934

Following gene: 15602932

Centisome position: 55.77

GC content: 45.49

Gene sequence:

>543_bases
ATGTCACCTGTTTATTATTGCTATGAGGATTCTCCGGTTGGGCGTTTATTGATGCTGTGTGAAGCGGAGAAACTTACGCA
TCTTGATTTTGTTGTCACGCAAGTTGCTCCTCGCCCCGATTGGATTTTAGATGAAAATCGACCGCTCTTTTTACAGGTTA
AGCAAGCCTTGCGGGACTATTTTGCTGGGAAACGCGTGTGCTTTGATGCGATTCCTTTCGCGCCTAAAGGTACGGCATTT
CAGCAACGCGTTTGGTCTGCATTACAACAGTTAGCTTATGGTGAAAGCACGACTTATGGCGAGCTGGCTCAGCAAATTGG
TCAGCCGAAGGCGGTGCGTGCGGTTGGTGGGGCCGTTGGTCGTAATCCAATTAGCATTCTGATTCCTTGTCATCGTGTTT
TAGGTAAACAACGTGAATTGACCGGTTTTGGTGGAGGCTTACCCATCAAACGCCATTTGTTGCAATTAGAACAAATTGAC
TATGTGGATCACGGGGTAGAGTTTGTAAAACCTAAACACCTCAAAAAATATCAGCAAAAATGA

Upstream 100 bases:

>100_bases
CGAACCTTTATGGTTTAAACGTGAATTACCGTTTCTAATTGACAAAAAATCAAGCATAATAAAGCGTACTTCGGTACGCT
TTTCTTTTTTAGAGGATGTT

Downstream 100 bases:

>100_bases
CCGCACTTTATGACACCTCAAACTGAGCAAGAATTATTACAACGGGCACAGGCGATTGCAGGCTTACGCTTTGCTGAGCT
GGCACAAAGCCTGCATATGC

Product: Dat1

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 180; Mature: 179

Protein sequence:

>180_residues
MSPVYYCYEDSPVGRLLMLCEAEKLTHLDFVVTQVAPRPDWILDENRPLFLQVKQALRDYFAGKRVCFDAIPFAPKGTAF
QQRVWSALQQLAYGESTTYGELAQQIGQPKAVRAVGGAVGRNPISILIPCHRVLGKQRELTGFGGGLPIKRHLLQLEQID
YVDHGVEFVKPKHLKKYQQK

Sequences:

>Translated_180_residues
MSPVYYCYEDSPVGRLLMLCEAEKLTHLDFVVTQVAPRPDWILDENRPLFLQVKQALRDYFAGKRVCFDAIPFAPKGTAF
QQRVWSALQQLAYGESTTYGELAQQIGQPKAVRAVGGAVGRNPISILIPCHRVLGKQRELTGFGGGLPIKRHLLQLEQID
YVDHGVEFVKPKHLKKYQQK
>Mature_179_residues
SPVYYCYEDSPVGRLLMLCEAEKLTHLDFVVTQVAPRPDWILDENRPLFLQVKQALRDYFAGKRVCFDAIPFAPKGTAFQ
QRVWSALQQLAYGESTTYGELAQQIGQPKAVRAVGGAVGRNPISILIPCHRVLGKQRELTGFGGGLPIKRHLLQLEQIDY
VDHGVEFVKPKHLKKYQQK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=161, Percent_Identity=32.9192546583851, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=47.6190476190476, Blast_Score=107, Evalue=4e-25,
Organism=Escherichia coli, GI1788542, Length=115, Percent_Identity=41.7391304347826, Blast_Score=89, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=46.25, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=46.25, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=44.4444444444444, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137554, Length=106, Percent_Identity=42.4528301886792, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20366; Mature: 20235

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVYYCYEDSPVGRLLMLCEAEKLTHLDFVVTQVAPRPDWILDENRPLFLQVKQALRDY
CCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHH
FAGKRVCFDAIPFAPKGTAFQQRVWSALQQLAYGESTTYGELAQQIGQPKAVRAVGGAVG
HCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCC
RNPISILIPCHRVLGKQRELTGFGGGLPIKRHLLQLEQIDYVDHGVEFVKPKHLKKYQQK
CCCEEEEEECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHCC
>Mature Secondary Structure 
SPVYYCYEDSPVGRLLMLCEAEKLTHLDFVVTQVAPRPDWILDENRPLFLQVKQALRDY
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHH
FAGKRVCFDAIPFAPKGTAFQQRVWSALQQLAYGESTTYGELAQQIGQPKAVRAVGGAVG
HCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHCC
RNPISILIPCHRVLGKQRELTGFGGGLPIKRHLLQLEQIDYVDHGVEFVKPKHLKKYQQK
CCCEEEEEECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]