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The map label for this gene is eptA [H]

Identifier: 15602907

GI number: 15602907

Start: 1228616

End: 1230247

Strand: Reverse

Name: eptA [H]

Synonym: PM1042

Alternate gene names: 15602907

Gene position: 1230247-1228616 (Counterclockwise)

Preceding gene: 15602908

Following gene: 15602906

Centisome position: 54.5

GC content: 40.62

Gene sequence:

>1632_bases
ATGAAAAAATTACGATTTCGTTGGTCCTTATCGTCCAGCAAATTACTTGCCCTCACCGCTTTCTACTTTAGTGTCATTTT
AAATTACCCTTTCTATGCCAAAGTACTGAGTATCCACCCTTTAACCCACACCTCTGCCGATTATTTTATTTACACCATGC
CACTGGTGATTTTCGTGATTCTCAATGCGGCTTTCCAAATCATTGCCATACCTGTCATACATAAGATCATCATGCCTTGT
TTGATCGTAATCAGTGCAGCCATTTCTTATAACACGTTATTTTTTGATATTTATTTTAATCGTGAGATGTTGCAAAACGT
AGTGCAAAGTAATCTGGCAGAAGGTTCACGCTTGATCACATGGACTTATCTAGAATGGCTGTTGGCGTTTGGTTTTGTGC
CGGCACTGTTATATTGTCTCACTCGTGTTAACTATCATCATGTCGTGAAAGAAGTGCTTGTTCGCTTGAGCATGATCTTA
CTTTCAGTTTTGTTATTTGTTGGTGTGGCAGCGTTTTTCTACCAAGATTATTCCGCCTTTTTCCGCAATAACAAAAGCCT
GACGCATTTATTAGTACCTTCCAATTTCATTGGCGCGACGATTAATGTGATACAGGATTTACGCGCGGCGAATTTACCTT
ATGTGCAACTTGATTTGAAAGCAAGCCAAGCAAAAACGGATCAAGATCGTCATGTCACAGTTTTAATCATTGGCGAAACG
ACACGTGCGAAAAATTGGGGGCTTAACCATTATGAGCGACAAACCACCCCGTTATTAGCAAATCGGGGCGAGGACGTGAT
TAACTTTCAGCATATGACGAGCTGTGGAACCTCAACTGCAGTTTCTGTCCCCTGTATGTTTTCCAGCTTAGATCGTAAAC
ACTTCAGCAATGTACAAGCGGATCACCAAGATAATTTACTGGATATTTTGCAACGCGCGGGGATTGCTATTAAATGGTTG
AATAATAATTCCGATTGTAAAGGCGTCTGTAAACACATTCCTTATCAGAATGTGACAACATTGAATTTAGCGGAATACTG
TCGAAATGGAGAATGTTTAGATAATATTTTACTGACGCAAGTGGATCAGATCCTTGACAGTACAGACAAGGATACAGTGC
TTGTTTTGCATACTATCGGTAATCATGGACCTACTTATTATGAACGTTATACGCCCGAATATCGCCAATTTGTGCCAACC
TGTGATACTAACCAAATCAATCGCTGTTCAAAACAACAACTGGTGAATACTTATGACAATGGGATTTTGTATGTAGACCA
ATTTATTGATCAAGTGATCAGCAAGCTAGAGCAGCGCCAACACTTACAGAGTGCTGTGATTTACGTGTCTGATCATGGCG
AATCCTTAGGGGAAAAAGGTGTTTATTTACATGCCGCGCCCTATGTGATTGCCCCAACAGAGCAGACACATATTCCGATG
ATCATGTGGTTTTCACCACGTTGGAAAGCCTCACAACAAGTGGATTTAACTTGTTTACGCCAAAAAGCAAAAGAAGAAGC
CTATTCGCATGATCACTTTTTTAGTACCGTGTTTGGTTTGATGCAAATGTCGGAGGAGAGTGAAACGTACCAACAAGACA
TGGATATTTTACGTCGTTGTCGCATACGCTAG

Upstream 100 bases:

>100_bases
ACTTGGCATGTTTGTTATATAAGCAAACAAAATAACACAGCAGAACTTTTTCATTTTTTTTGTTTCTAACAGAAGTCGAT
TTTTTTAGGAGTTTTGTTGT

Downstream 100 bases:

>100_bases
TATAGGGCATTAAATAAAAGCCAAGTAAAGCTTGGCTTTTTTACTGGCTTTTTATACAGACTTTCATTGGAAATGCCGTC
GATTTAGGCTAAACTATCGC

Product: hypothetical protein

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MKKLRFRWSLSSSKLLALTAFYFSVILNYPFYAKVLSIHPLTHTSADYFIYTMPLVIFVILNAAFQIIAIPVIHKIIMPC
LIVISAAISYNTLFFDIYFNREMLQNVVQSNLAEGSRLITWTYLEWLLAFGFVPALLYCLTRVNYHHVVKEVLVRLSMIL
LSVLLFVGVAAFFYQDYSAFFRNNKSLTHLLVPSNFIGATINVIQDLRAANLPYVQLDLKASQAKTDQDRHVTVLIIGET
TRAKNWGLNHYERQTTPLLANRGEDVINFQHMTSCGTSTAVSVPCMFSSLDRKHFSNVQADHQDNLLDILQRAGIAIKWL
NNNSDCKGVCKHIPYQNVTTLNLAEYCRNGECLDNILLTQVDQILDSTDKDTVLVLHTIGNHGPTYYERYTPEYRQFVPT
CDTNQINRCSKQQLVNTYDNGILYVDQFIDQVISKLEQRQHLQSAVIYVSDHGESLGEKGVYLHAAPYVIAPTEQTHIPM
IMWFSPRWKASQQVDLTCLRQKAKEEAYSHDHFFSTVFGLMQMSEESETYQQDMDILRRCRIR

Sequences:

>Translated_543_residues
MKKLRFRWSLSSSKLLALTAFYFSVILNYPFYAKVLSIHPLTHTSADYFIYTMPLVIFVILNAAFQIIAIPVIHKIIMPC
LIVISAAISYNTLFFDIYFNREMLQNVVQSNLAEGSRLITWTYLEWLLAFGFVPALLYCLTRVNYHHVVKEVLVRLSMIL
LSVLLFVGVAAFFYQDYSAFFRNNKSLTHLLVPSNFIGATINVIQDLRAANLPYVQLDLKASQAKTDQDRHVTVLIIGET
TRAKNWGLNHYERQTTPLLANRGEDVINFQHMTSCGTSTAVSVPCMFSSLDRKHFSNVQADHQDNLLDILQRAGIAIKWL
NNNSDCKGVCKHIPYQNVTTLNLAEYCRNGECLDNILLTQVDQILDSTDKDTVLVLHTIGNHGPTYYERYTPEYRQFVPT
CDTNQINRCSKQQLVNTYDNGILYVDQFIDQVISKLEQRQHLQSAVIYVSDHGESLGEKGVYLHAAPYVIAPTEQTHIPM
IMWFSPRWKASQQVDLTCLRQKAKEEAYSHDHFFSTVFGLMQMSEESETYQQDMDILRRCRIR
>Mature_543_residues
MKKLRFRWSLSSSKLLALTAFYFSVILNYPFYAKVLSIHPLTHTSADYFIYTMPLVIFVILNAAFQIIAIPVIHKIIMPC
LIVISAAISYNTLFFDIYFNREMLQNVVQSNLAEGSRLITWTYLEWLLAFGFVPALLYCLTRVNYHHVVKEVLVRLSMIL
LSVLLFVGVAAFFYQDYSAFFRNNKSLTHLLVPSNFIGATINVIQDLRAANLPYVQLDLKASQAKTDQDRHVTVLIIGET
TRAKNWGLNHYERQTTPLLANRGEDVINFQHMTSCGTSTAVSVPCMFSSLDRKHFSNVQADHQDNLLDILQRAGIAIKWL
NNNSDCKGVCKHIPYQNVTTLNLAEYCRNGECLDNILLTQVDQILDSTDKDTVLVLHTIGNHGPTYYERYTPEYRQFVPT
CDTNQINRCSKQQLVNTYDNGILYVDQFIDQVISKLEQRQHLQSAVIYVSDHGESLGEKGVYLHAAPYVIAPTEQTHIPM
IMWFSPRWKASQQVDLTCLRQKAKEEAYSHDHFFSTVFGLMQMSEESETYQQDMDILRRCRIR

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=546, Percent_Identity=42.4908424908425, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI87082286, Length=556, Percent_Identity=24.2805755395683, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI87082223, Length=477, Percent_Identity=25.9958071278826, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790392, Length=462, Percent_Identity=20.7792207792208, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 62425; Mature: 62425

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLRFRWSLSSSKLLALTAFYFSVILNYPFYAKVLSIHPLTHTSADYFIYTMPLVIFVI
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCCHHHHHHHHHHHHH
LNAAFQIIAIPVIHKIIMPCLIVISAAISYNTLFFDIYFNREMLQNVVQSNLAEGSRLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECHHHHHHHHHHHHCCCCEEEH
WTYLEWLLAFGFVPALLYCLTRVNYHHVVKEVLVRLSMILLSVLLFVGVAAFFYQDYSAF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRNNKSLTHLLVPSNFIGATINVIQDLRAANLPYVQLDLKASQAKTDQDRHVTVLIIGET
HCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCEEEEEEEECC
TRAKNWGLNHYERQTTPLLANRGEDVINFQHMTSCGTSTAVSVPCMFSSLDRKHFSNVQA
CCCCCCCCCCHHHCCCCHHHCCCCCCEEHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCC
DHQDNLLDILQRAGIAIKWLNNNSDCKGVCKHIPYQNVTTLNLAEYCRNGECLDNILLTQ
CCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHH
VDQILDSTDKDTVLVLHTIGNHGPTYYERYTPEYRQFVPTCDTNQINRCSKQQLVNTYDN
HHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCC
GILYVDQFIDQVISKLEQRQHLQSAVIYVSDHGESLGEKGVYLHAAPYVIAPTEQTHIPM
CEEEHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCEEEECCCCCCCCE
IMWFSPRWKASQQVDLTCLRQKAKEEAYSHDHFFSTVFGLMQMSEESETYQQDMDILRRC
EEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIR
CCC
>Mature Secondary Structure
MKKLRFRWSLSSSKLLALTAFYFSVILNYPFYAKVLSIHPLTHTSADYFIYTMPLVIFVI
CCCEEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCCHHHHHHHHHHHHH
LNAAFQIIAIPVIHKIIMPCLIVISAAISYNTLFFDIYFNREMLQNVVQSNLAEGSRLIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECHHHHHHHHHHHHCCCCEEEH
WTYLEWLLAFGFVPALLYCLTRVNYHHVVKEVLVRLSMILLSVLLFVGVAAFFYQDYSAF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRNNKSLTHLLVPSNFIGATINVIQDLRAANLPYVQLDLKASQAKTDQDRHVTVLIIGET
HCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCCEEEEEEEECC
TRAKNWGLNHYERQTTPLLANRGEDVINFQHMTSCGTSTAVSVPCMFSSLDRKHFSNVQA
CCCCCCCCCCHHHCCCCHHHCCCCCCEEHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCC
DHQDNLLDILQRAGIAIKWLNNNSDCKGVCKHIPYQNVTTLNLAEYCRNGECLDNILLTQ
CCCCCHHHHHHHCCEEEEEECCCCCHHHHHHHCCCCCCEEEEHHHHCCCCCHHHHHHHHH
VDQILDSTDKDTVLVLHTIGNHGPTYYERYTPEYRQFVPTCDTNQINRCSKQQLVNTYDN
HHHHHCCCCCCEEEEEEECCCCCCCHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHCC
GILYVDQFIDQVISKLEQRQHLQSAVIYVSDHGESLGEKGVYLHAAPYVIAPTEQTHIPM
CEEEHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCEEEEECCEEEECCCCCCCCE
IMWFSPRWKASQQVDLTCLRQKAKEEAYSHDHFFSTVFGLMQMSEESETYQQDMDILRRC
EEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RIR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]