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The map label for this gene is metX [H]

Identifier: 15602892

GI number: 15602892

Start: 1209293

End: 1210447

Strand: Reverse

Name: metX [H]

Synonym: PM1027

Alternate gene names: 15602892

Gene position: 1210447-1209293 (Counterclockwise)

Preceding gene: 15602893

Following gene: 15602891

Centisome position: 53.62

GC content: 43.72

Gene sequence:

>1155_bases
ATGTCTCGTTATTTATTCACTTCCGAATCCGTATCAGAAGGACATCCAGATAAAATTGCCGATCAAATTTCCGATGCGGT
ATTAGACGAAATTTTAAAACAAGATCCCAAAGCCCGCGTTGCTTGTGAAACCTATGTAAAAACAGGGATGGCATTAGTGG
GTGGCGAGATCACAACATCGGCTTGGGTAGATATTGAAAACTTAACCCGCCAGGTGATTTGTGATATTGGCTATAAACAT
TCTGATATGGGCTTTGATGGTCATTCTTGTGCCGTGTTAAATGCAATTGGTAAACAATCTTCCGATATTAATCAAGGTGT
GGATCGTGAAAATCCATTAGATCAAGGTGCTGGTGACCAAGGGATTATGTTTGGTTATGCCACTAATGAAACTGAAGTGC
TGATGCCTGCAGCCATTACTTATGCGCACCGTTTAATGGAACGTCAAGCCAAGGTGCGTAAAGAGGGTACCTTGCCATGG
TTACGTCCTGATGCAAAAAGCCAAGTCACGCTAAAATATGAAGATCACAAAATTGTTGGGGTCGATGCGGTCGTCCTTTC
TACGCAACACTGTGACAGCATTTCACAACATGATTTGCATGAAGCAGTGATGGAAGAAATTATTAAGCCTGTTTTACCTG
CAGAGTGGTTATCAAAAGAAACCAAATATTTCATCAACCCAACAGGACGCTTTGTGATTGGTGGTCCGATGGGTGACTGC
GGGTTAACAGGGCGTAAAATTATTGTGGATACTTACGGCGGTGCCGCGCGTCATGGTGGCGGTGCATTCTCGGGTAAAGA
TCCTTCCAAAGTAGACCGCTCAGCTGCTTATGCGGCGCGTTATGTGGCGAAAAATATTGTTGCTGCGGGGCTTGCAGATC
GTTGCGAAATTCAACTTTCGTATGCGATTGGGGTTGCTGATCCGACGTCTATCATGGTGGAAACTTTTGGTACCGGTAAA
GTTGCTAACGAATTATTAGTAGCATTGGTGCGTGAATTCTTTGATTTACGTCCGTACGGTTTAATTAAAATGCTTGATTT
AATTCAGCCTATTTACCGTGAAACGGCGGCTTATGGTCACTTTGGGCGTGAACAATTCCCATGGGAAAAAGTTGATCGTG
CAGAAGAATTACGCGCTGCAGCAGGACTTAAATAA

Upstream 100 bases:

>100_bases
GAAGGGAAATTTTTTTGCGATAAAGATTGAATTTTCTCAATAAAAGCGTATTATAGCCGTCCAGACGCAGAAACATCTAA
AAGAACACAAGAAGGATATT

Downstream 100 bases:

>100_bases
CCGTTACTCGGACAAGAAACGTAAAGGCGGACAACAACGTTCGCCTTCTTTTTTATGCCAATTATGCAACCACAAACTCA
GCTTCGTCATTTAAATAGGC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSRYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYKH
SDMGFDGHSCAVLNAIGKQSSDINQGVDRENPLDQGAGDQGIMFGYATNETEVLMPAAITYAHRLMERQAKVRKEGTLPW
LRPDAKSQVTLKYEDHKIVGVDAVVLSTQHCDSISQHDLHEAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGK
VANELLVALVREFFDLRPYGLIKMLDLIQPIYRETAAYGHFGREQFPWEKVDRAEELRAAAGLK

Sequences:

>Translated_384_residues
MSRYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYKH
SDMGFDGHSCAVLNAIGKQSSDINQGVDRENPLDQGAGDQGIMFGYATNETEVLMPAAITYAHRLMERQAKVRKEGTLPW
LRPDAKSQVTLKYEDHKIVGVDAVVLSTQHCDSISQHDLHEAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGDC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGK
VANELLVALVREFFDLRPYGLIKMLDLIQPIYRETAAYGHFGREQFPWEKVDRAEELRAAAGLK
>Mature_383_residues
SRYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTSAWVDIENLTRQVICDIGYKHS
DMGFDGHSCAVLNAIGKQSSDINQGVDRENPLDQGAGDQGIMFGYATNETEVLMPAAITYAHRLMERQAKVRKEGTLPWL
RPDAKSQVTLKYEDHKIVGVDAVVLSTQHCDSISQHDLHEAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGDCG
LTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLSYAIGVADPTSIMVETFGTGKV
ANELLVALVREFFDLRPYGLIKMLDLIQPIYRETAAYGHFGREQFPWEKVDRAEELRAAAGLK

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=372, Percent_Identity=56.7204301075269, Blast_Score=405, Evalue=1e-113,
Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=57.258064516129, Blast_Score=403, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=384, Percent_Identity=83.3333333333333, Blast_Score=676, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17551082, Length=374, Percent_Identity=56.4171122994652, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=57.2192513368984, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=374, Percent_Identity=56.6844919786096, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145633, Length=390, Percent_Identity=55.1282051282051, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32565905, Length=374, Percent_Identity=50.8021390374332, Blast_Score=340, Evalue=5e-94,
Organism=Caenorhabditis elegans, GI32565911, Length=327, Percent_Identity=54.434250764526, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI25145635, Length=340, Percent_Identity=52.3529411764706, Blast_Score=324, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI32565903, Length=303, Percent_Identity=53.4653465346535, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI32565909, Length=85, Percent_Identity=62.3529411764706, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI32565907, Length=46, Percent_Identity=69.5652173913043, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=377, Percent_Identity=56.4986737400531, Blast_Score=411, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6323209, Length=372, Percent_Identity=57.258064516129, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45552159, Length=377, Percent_Identity=54.3766578249337, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580529, Length=377, Percent_Identity=54.3766578249337, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580537, Length=377, Percent_Identity=54.3766578249337, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580533, Length=377, Percent_Identity=54.3766578249337, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580535, Length=377, Percent_Identity=54.3766578249337, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580531, Length=377, Percent_Identity=54.3766578249337, Blast_Score=372, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=55.2278820375335, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=55.2278820375335, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=52.9411764705882, Blast_Score=347, Evalue=1e-95,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=57.1428571428571, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42058; Mature: 41927

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTS
CCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHCCCCCCHH
AWVDIENLTRQVICDIGYKHSDMGFDGHSCAVLNAIGKQSSDINQGVDRENPLDQGAGDQ
HEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCC
GIMFGYATNETEVLMPAAITYAHRLMERQAKVRKEGTLPWLRPDAKSQVTLKYEDHKIVG
CEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEE
VDAVVLSTQHCDSISQHDLHEAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGDC
EHEEEEECHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTGKVANELLVALVREFFDLRPYGLIKMLDLIQPIYRETAAYGH
EEEECCCCHHEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
FGREQFPWEKVDRAEELRAAAGLK
CCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRYLFTSESVSEGHPDKIADQISDAVLDEILKQDPKARVACETYVKTGMALVGGEITTS
CCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHCCCCCCHH
AWVDIENLTRQVICDIGYKHSDMGFDGHSCAVLNAIGKQSSDINQGVDRENPLDQGAGDQ
HEEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCC
GIMFGYATNETEVLMPAAITYAHRLMERQAKVRKEGTLPWLRPDAKSQVTLKYEDHKIVG
CEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEE
VDAVVLSTQHCDSISQHDLHEAVMEEIIKPVLPAEWLSKETKYFINPTGRFVIGGPMGDC
EHEEEEECHHHCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGAARHGGGAFSGKDPSKVDRSAAYAARYVAKNIVAAGLADRCEIQLS
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
YAIGVADPTSIMVETFGTGKVANELLVALVREFFDLRPYGLIKMLDLIQPIYRETAAYGH
EEEECCCCHHEEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
FGREQFPWEKVDRAEELRAAAGLK
CCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA