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The map label for this gene is cobB

Identifier: 15602858

GI number: 15602858

Start: 1166840

End: 1167544

Strand: Direct

Name: cobB

Synonym: PM0993

Alternate gene names: 15602858

Gene position: 1166840-1167544 (Clockwise)

Preceding gene: 15602857

Following gene: 15602867

Centisome position: 51.69

GC content: 42.98

Gene sequence:

>705_bases
ATGACAAAACAAGTTAGAATTGTTGTCTTAACCGGTGCAGGCATTTCAGCCGAATCGGGTATTCGCACTTTCCGAGCTAC
TGATGGCTTATGGGAGAATCATCCTGTTGATGAGGTTGCTACCCCAGAAGGCTTTGCTCGCAATCCCAAACTCGTGCAGC
GCTTTTACAATGAACGCCGCAAGCAGTTATTTTCTGATCAGATTGCACCCAATGCTGCCCATTTCGCCTTAGCGGAACTG
GAGAAAAAACTCGGCGATAATTTACTCATCGTCACACAAAATGTAGATAATTTACACGAACGTGCAGGGTCAAAAAACTT
GATTCATATGCATGGGGAATTATTGAAAGTTCGCTGTGTCAAATCAGGAAAAATTTATGACTGGCAAGGAGATATTGGTG
AGCACGACAAGTGTCTTTGCTGTACACCTACTCAGATTCTGCGTCCACATATTGTATGGTTTGGCGAAATGCCGTTAGAA
ATGGAAAAAATTCAGACCGCACTTTCTGAGTGTGATTATTTTATTTCAATTGGAACGTCTGGCAATGTTTATCCCGCAGC
AGGGTTTGTACGCGAAGCGCTGTTTTATGGGGCTCACACAGTCGAGCTTAATTTAGAACCTAGCCAAGTGCGGTCTAGTT
TTGACGAATGTCATTATGGTAAAGCGACGGAATTGGTTCCACACTATTTAGCACAATTTCTTTAA

Upstream 100 bases:

>100_bases
AGCAAAAATCAAGCAATTAATATCATAACAACAAAAAATGACGGGGAAATACGAGTGAAATCTTGTTTGAGGTAAGAACC
ACACATCAGGAGGAAAACGC

Downstream 100 bases:

>100_bases
ACATAAAAAAATCCCAAACACACCTGTTTGGGATTTTTTATGCTATTCGTTAACTAGATATTCTGATTAATAAAGTTAGC
TAATTGGTTCTTTGGTAAAG

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MTKQVRIVVLTGAGISAESGIRTFRATDGLWENHPVDEVATPEGFARNPKLVQRFYNERRKQLFSDQIAPNAAHFALAEL
EKKLGDNLLIVTQNVDNLHERAGSKNLIHMHGELLKVRCVKSGKIYDWQGDIGEHDKCLCCTPTQILRPHIVWFGEMPLE
MEKIQTALSECDYFISIGTSGNVYPAAGFVREALFYGAHTVELNLEPSQVRSSFDECHYGKATELVPHYLAQFL

Sequences:

>Translated_234_residues
MTKQVRIVVLTGAGISAESGIRTFRATDGLWENHPVDEVATPEGFARNPKLVQRFYNERRKQLFSDQIAPNAAHFALAEL
EKKLGDNLLIVTQNVDNLHERAGSKNLIHMHGELLKVRCVKSGKIYDWQGDIGEHDKCLCCTPTQILRPHIVWFGEMPLE
MEKIQTALSECDYFISIGTSGNVYPAAGFVREALFYGAHTVELNLEPSQVRSSFDECHYGKATELVPHYLAQFL
>Mature_233_residues
TKQVRIVVLTGAGISAESGIRTFRATDGLWENHPVDEVATPEGFARNPKLVQRFYNERRKQLFSDQIAPNAAHFALAELE
KKLGDNLLIVTQNVDNLHERAGSKNLIHMHGELLKVRCVKSGKIYDWQGDIGEHDKCLCCTPTQILRPHIVWFGEMPLEM
EKIQTALSECDYFISIGTSGNVYPAAGFVREALFYGAHTVELNLEPSQVRSSFDECHYGKATELVPHYLAQFL

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain

Homologues:

Organism=Homo sapiens, GI300795542, Length=245, Percent_Identity=37.5510204081633, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI6912664, Length=258, Percent_Identity=36.046511627907, Blast_Score=157, Evalue=7e-39,
Organism=Homo sapiens, GI13787215, Length=242, Percent_Identity=36.7768595041322, Blast_Score=155, Evalue=2e-38,
Organism=Homo sapiens, GI7657575, Length=220, Percent_Identity=30.4545454545455, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI6912662, Length=247, Percent_Identity=30.3643724696356, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI308199517, Length=229, Percent_Identity=66.3755458515284, Blast_Score=327, Evalue=6e-91,
Organism=Caenorhabditis elegans, GI17541892, Length=195, Percent_Identity=31.7948717948718, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71990482, Length=242, Percent_Identity=28.099173553719, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17567771, Length=119, Percent_Identity=37.8151260504202, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6325242, Length=221, Percent_Identity=29.8642533936652, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137536, Length=224, Percent_Identity=29.0178571428571, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648389, Length=202, Percent_Identity=30.6930693069307, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571445, Length=120, Percent_Identity=40, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NPD_PASMU (Q9CM50)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245930.1
- ProteinModelPortal:   Q9CM50
- SMR:   Q9CM50
- GeneID:   1244340
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0993
- NMPDR:   fig|272843.1.peg.993
- HOGENOM:   HBG641281
- OMA:   FVHEARL
- ProtClustDB:   PRK00481
- BioCyc:   PMUL272843:PM0993-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01121
- InterPro:   IPR003000
- PANTHER:   PTHR11085

Pfam domain/function: PF02146 SIR2

EC number: 3.5.1.- [C]

Molecular weight: Translated: 26343; Mature: 26212

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS50305 SIRTUIN

Important sites: ACT_SITE 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQVRIVVLTGAGISAESGIRTFRATDGLWENHPVDEVATPEGFARNPKLVQRFYNERR
CCCEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KQLFSDQIAPNAAHFALAELEKKLGDNLLIVTQNVDNLHERAGSKNLIHMHGELLKVRCV
HHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEECCCEEEEEEE
KSGKIYDWQGDIGEHDKCLCCTPTQILRPHIVWFGEMPLEMEKIQTALSECDYFISIGTS
CCCCEEECCCCCCCCCCEEEECCHHHHCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCC
GNVYPAAGFVREALFYGAHTVELNLEPSQVRSSFDECHYGKATELVPHYLAQFL
CCCCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
TKQVRIVVLTGAGISAESGIRTFRATDGLWENHPVDEVATPEGFARNPKLVQRFYNERR
CCEEEEEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KQLFSDQIAPNAAHFALAELEKKLGDNLLIVTQNVDNLHERAGSKNLIHMHGELLKVRCV
HHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCCCEEEECCCEEEEEEE
KSGKIYDWQGDIGEHDKCLCCTPTQILRPHIVWFGEMPLEMEKIQTALSECDYFISIGTS
CCCCEEECCCCCCCCCCEEEECCHHHHCCEEEEECCCCCHHHHHHHHHHCCCEEEEECCC
GNVYPAAGFVREALFYGAHTVELNLEPSQVRSSFDECHYGKATELVPHYLAQFL
CCCCCHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100