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The map label for this gene is gloB

Identifier: 15602550

GI number: 15602550

Start: 795547

End: 796248

Strand: Reverse

Name: gloB

Synonym: PM0685

Alternate gene names: 15602550

Gene position: 796248-795547 (Counterclockwise)

Preceding gene: 15602555

Following gene: 15602549

Centisome position: 35.27

GC content: 40.46

Gene sequence:

>702_bases
ATGCTAGTACCGATTCCAGCTCTAAATGATAACTACATTTGGCTCTATGGACGAGCAAATTTACCGATAATTGTGATCGA
TATACCAGAATTTACCCCGTTACTCGATTATGTTCAACAGCATCAACTTAACGTGGAAGCCTTGCTGTTAACTCATCATC
ATGATGATCACACTGCTGGGGTCTCACTTTTTAAACAACATTTTCCAAACGTGAAAGTCTTTGGACCAGCAGAAACTCAA
GCGAAAGGGGCGACTGAAATTGTTAATATGGGCATTTTGCAGACCGAACATTACCACATTCACGTATTACAAACCGCTGG
ACATACGGAACAACATGTGAGTTATCTGGTTGATGGGCATTTATTTTGTGGCGACAGTTTATTTTCTGCGGGCTGTGGAC
GTGTTTTTACCGGGAATTATCAACATATGTTTGATGGTTTACAACGCTTAAAATCCTTGCCTGATGACACAATTGTGTGT
CCCGCCCATGAATATACTTTAGCCAATTTGGCCTTTGCCAAATATATATTGCCAGAAAATGTAGCGATACAACAACATGA
ACAATGGGTACAAAAACAGCGTGTAACACACCAGCCAAGTTTACCCACGACAATGGGACGAGAAAAACAGATTAATCCGT
TTTTGATCGCACAAGATATGGAAACCTTTGTTGCATGGCGTAAAGCAAAAGACGTGTTTTAA

Upstream 100 bases:

>100_bases
ATAGCAACTGCCATTTTGTAATTGTTGCCAGCTGTGCGGCAAGTGCATGACGTTAGGATATTTTGCGTTCCACCACATGA
CAACCCCTTGAGGACAATTT

Downstream 100 bases:

>100_bases
TTGAAATAAAAGCGAAGTTCATGTAAATAATCACGTTGTCGTTTGCTCATGGCAGGACAATTGATTAAAATGGACCTTAT
TTCTTTTCTTTGTGGGTTCA

Product: hypothetical protein

Products: NA

Alternate protein names: Glyoxalase II; Glx II

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MLVPIPALNDNYIWLYGRANLPIIVIDIPEFTPLLDYVQQHQLNVEALLLTHHHDDHTAGVSLFKQHFPNVKVFGPAETQ
AKGATEIVNMGILQTEHYHIHVLQTAGHTEQHVSYLVDGHLFCGDSLFSAGCGRVFTGNYQHMFDGLQRLKSLPDDTIVC
PAHEYTLANLAFAKYILPENVAIQQHEQWVQKQRVTHQPSLPTTMGREKQINPFLIAQDMETFVAWRKAKDVF

Sequences:

>Translated_233_residues
MLVPIPALNDNYIWLYGRANLPIIVIDIPEFTPLLDYVQQHQLNVEALLLTHHHDDHTAGVSLFKQHFPNVKVFGPAETQ
AKGATEIVNMGILQTEHYHIHVLQTAGHTEQHVSYLVDGHLFCGDSLFSAGCGRVFTGNYQHMFDGLQRLKSLPDDTIVC
PAHEYTLANLAFAKYILPENVAIQQHEQWVQKQRVTHQPSLPTTMGREKQINPFLIAQDMETFVAWRKAKDVF
>Mature_233_residues
MLVPIPALNDNYIWLYGRANLPIIVIDIPEFTPLLDYVQQHQLNVEALLLTHHHDDHTAGVSLFKQHFPNVKVFGPAETQ
AKGATEIVNMGILQTEHYHIHVLQTAGHTEQHVSYLVDGHLFCGDSLFSAGCGRVFTGNYQHMFDGLQRLKSLPDDTIVC
PAHEYTLANLAFAKYILPENVAIQQHEQWVQKQRVTHQPSLPTTMGREKQINPFLIAQDMETFVAWRKAKDVF

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family

Homologues:

Organism=Homo sapiens, GI116642887, Length=229, Percent_Identity=36.2445414847162, Blast_Score=146, Evalue=1e-35,
Organism=Homo sapiens, GI21703352, Length=229, Percent_Identity=36.2445414847162, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI94538322, Length=250, Percent_Identity=30, Blast_Score=125, Evalue=2e-29,
Organism=Homo sapiens, GI94538320, Length=250, Percent_Identity=30, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI14150041, Length=219, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI46361987, Length=202, Percent_Identity=31.1881188118812, Blast_Score=102, Evalue=2e-22,
Organism=Escherichia coli, GI1786406, Length=221, Percent_Identity=42.0814479638009, Blast_Score=178, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17536925, Length=227, Percent_Identity=32.15859030837, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320478, Length=227, Percent_Identity=31.2775330396476, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356335, Length=221, Percent_Identity=34.841628959276, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24667703, Length=221, Percent_Identity=34.841628959276, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24667711, Length=252, Percent_Identity=32.9365079365079, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLO2_PASMU (Q9CMW8)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245622.1
- HSSP:   Q9SID3
- ProteinModelPortal:   Q9CMW8
- SMR:   Q9CMW8
- GeneID:   1244032
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0685
- NMPDR:   fig|272843.1.peg.685
- HOGENOM:   HBG753931
- OMA:   WCAHEYT
- ProtClustDB:   CLSK870202
- BioCyc:   PMUL272843:PM0685-MONOMER
- BRENDA:   3.1.2.6
- HAMAP:   MF_01374
- InterPro:   IPR001279
- InterPro:   IPR017782
- SMART:   SM00849
- TIGRFAMs:   TIGR03413

Pfam domain/function: PF00753 Lactamase_B

EC number: =3.1.2.6

Molecular weight: Translated: 26484; Mature: 26484

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVPIPALNDNYIWLYGRANLPIIVIDIPEFTPLLDYVQQHQLNVEALLLTHHHDDHTAG
CEECCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHH
VSLFKQHFPNVKVFGPAETQAKGATEIVNMGILQTEHYHIHVLQTAGHTEQHVSYLVDGH
HHHHHHHCCCEEEECCCCCCCCCHHHHHHHCEEEECCEEEEEEECCCCCHHHHHHHHCCE
LFCGDSLFSAGCGRVFTGNYQHMFDGLQRLKSLPDDTIVCPAHEYTLANLAFAKYILPEN
EEECCHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCC
VAIQQHEQWVQKQRVTHQPSLPTTMGREKQINPFLIAQDMETFVAWRKAKDVF
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLVPIPALNDNYIWLYGRANLPIIVIDIPEFTPLLDYVQQHQLNVEALLLTHHHDDHTAG
CEECCCCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCCHHHH
VSLFKQHFPNVKVFGPAETQAKGATEIVNMGILQTEHYHIHVLQTAGHTEQHVSYLVDGH
HHHHHHHCCCEEEECCCCCCCCCHHHHHHHCEEEECCEEEEEEECCCCCHHHHHHHHCCE
LFCGDSLFSAGCGRVFTGNYQHMFDGLQRLKSLPDDTIVCPAHEYTLANLAFAKYILPEN
EEECCHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCC
VAIQQHEQWVQKQRVTHQPSLPTTMGREKQINPFLIAQDMETFVAWRKAKDVF
CHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100