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The map label for this gene is moaA

Identifier: 15602490

GI number: 15602490

Start: 727449

End: 728462

Strand: Reverse

Name: moaA

Synonym: PM0625

Alternate gene names: 15602490

Gene position: 728462-727449 (Counterclockwise)

Preceding gene: 15602492

Following gene: 15602489

Centisome position: 32.27

GC content: 39.55

Gene sequence:

>1014_bases
ATGCAAACAATTCCAATTAAACATGTTGGTAATGATCCTTTAGTGGATGCGTTCCAACGTCGCTATTACTATTTGCGACT
GTCAATTACAGATGTGTGTAATTTCCGCTGTAATTACTGTTTGCCGAATGGTTATCAACCTGAGTCAAATAAACCCAGTT
TTCTCACCTTAAATGAAATTCGTTTAGTGGTAAAAAGTTTTGCTAACATGGGTACTGAAAAAGTCCGTATTACGGGGGGA
GAACCTACCTTACGTAAAGATTTTTTACCTATTGTTGAAACCATTGCTCAAAATCCTACTATTAAACAGATTGCTTTGAC
CACGAATGGTTACCGTATGGCAAAAGATGTTGCTGCGTGGAAAGAGGCGGGGATCACGTCGATTAACGTGAGTATTGATA
GCTTAGATGCACGAATGTTCCATCGTATTACCGGGATAGACAAATTTGAAGACGTAATGCGTGGTCTTGAGCGTGCTTTT
GAGGTGGGCTATCAGAAAATCAAAGTCAATTCCGTGTTAATGAGAGATCTTAATGACGCGGATTTTAATCAGTTTTTAAC
TTGGATTAAAGACAAGCCGATCCAAATGCGTTTTATTGAATTAATGCAAACGGGGGAAATGGATCATTTCTTTCAGCAAC
ATCATGTATCAGGTCAATTGTTAGCAGATAAATTAATCAATGCGGGGTGGACCTTACAAAGCAAGGGATTACTTGATGGA
CCGGCTAAAGTATTTAAACACCCAGATTATGTTGGCGAAGTAGGCTTGATTATGCCCTATGAGAAAAATTTCTGTGCGAG
CTGTAATCGTTTAAGGGTATCAGCGAAAGGCAAACTGCACTTATGTTTGTTTGGTGAAGAAGGCATTACGTTACGTGACT
TACTGCAATCGGATGATCAACAATTGCAATTACAAGCACGTATTTCTTCTGCACTACAAGGCAAACGTGAGCATCATTTC
TTACATCAAGGTGATAGTGGTGTACGCGCAAATTTAGCCAGTATCGGCGGTTAA

Upstream 100 bases:

>100_bases
TACAATAGAAATAAGGTGGTAATGCCAATAGCATAAAACTTGTGCTATTCAGGGATACGCTGGAAACGGTTTATCCTCTC
GTATTTTAGAAAGGTGTGAC

Downstream 100 bases:

>100_bases
TCATATTTTCTACAAATTGACCGCACTTTTTCTATTTTTCAACTTAACCATGAGAAATTATGAGTACATTTACCCATATT
AATTGCCAAGGGGAAGCCAA

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MQTIPIKHVGNDPLVDAFQRRYYYLRLSITDVCNFRCNYCLPNGYQPESNKPSFLTLNEIRLVVKSFANMGTEKVRITGG
EPTLRKDFLPIVETIAQNPTIKQIALTTNGYRMAKDVAAWKEAGITSINVSIDSLDARMFHRITGIDKFEDVMRGLERAF
EVGYQKIKVNSVLMRDLNDADFNQFLTWIKDKPIQMRFIELMQTGEMDHFFQQHHVSGQLLADKLINAGWTLQSKGLLDG
PAKVFKHPDYVGEVGLIMPYEKNFCASCNRLRVSAKGKLHLCLFGEEGITLRDLLQSDDQQLQLQARISSALQGKREHHF
LHQGDSGVRANLASIGG

Sequences:

>Translated_337_residues
MQTIPIKHVGNDPLVDAFQRRYYYLRLSITDVCNFRCNYCLPNGYQPESNKPSFLTLNEIRLVVKSFANMGTEKVRITGG
EPTLRKDFLPIVETIAQNPTIKQIALTTNGYRMAKDVAAWKEAGITSINVSIDSLDARMFHRITGIDKFEDVMRGLERAF
EVGYQKIKVNSVLMRDLNDADFNQFLTWIKDKPIQMRFIELMQTGEMDHFFQQHHVSGQLLADKLINAGWTLQSKGLLDG
PAKVFKHPDYVGEVGLIMPYEKNFCASCNRLRVSAKGKLHLCLFGEEGITLRDLLQSDDQQLQLQARISSALQGKREHHF
LHQGDSGVRANLASIGG
>Mature_337_residues
MQTIPIKHVGNDPLVDAFQRRYYYLRLSITDVCNFRCNYCLPNGYQPESNKPSFLTLNEIRLVVKSFANMGTEKVRITGG
EPTLRKDFLPIVETIAQNPTIKQIALTTNGYRMAKDVAAWKEAGITSINVSIDSLDARMFHRITGIDKFEDVMRGLERAF
EVGYQKIKVNSVLMRDLNDADFNQFLTWIKDKPIQMRFIELMQTGEMDHFFQQHHVSGQLLADKLINAGWTLQSKGLLDG
PAKVFKHPDYVGEVGLIMPYEKNFCASCNRLRVSAKGKLHLCLFGEEGITLRDLLQSDDQQLQLQARISSALQGKREHHF
LHQGDSGVRANLASIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=308, Percent_Identity=35.3896103896104, Blast_Score=204, Evalue=9e-53,
Organism=Homo sapiens, GI22027536, Length=308, Percent_Identity=35.3896103896104, Blast_Score=204, Evalue=1e-52,
Organism=Escherichia coli, GI1786999, Length=325, Percent_Identity=61.8461538461539, Blast_Score=449, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI32566331, Length=318, Percent_Identity=37.7358490566038, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI32566329, Length=318, Percent_Identity=37.7358490566038, Blast_Score=213, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17561210, Length=314, Percent_Identity=36.9426751592357, Blast_Score=211, Evalue=5e-55,
Organism=Drosophila melanogaster, GI28574490, Length=322, Percent_Identity=36.0248447204969, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI28574488, Length=314, Percent_Identity=36.3057324840764, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_PASMU (Q9CN21)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245562.1
- ProteinModelPortal:   Q9CN21
- SMR:   Q9CN21
- GeneID:   1243972
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0625
- NMPDR:   fig|272843.1.peg.625
- HOGENOM:   HBG529885
- OMA:   IDSLDAR
- ProtClustDB:   PRK00164
- BioCyc:   PMUL272843:PM0625-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 38209; Mature: 38209

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 26-26 BINDING 39-39 BINDING 76-76 BINDING 80-80 BINDING 107-107 BINDING 131-131 BINDING 168-168 BINDING 202-202

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTIPIKHVGNDPLVDAFQRRYYYLRLSITDVCNFRCNYCLPNGYQPESNKPSFLTLNEI
CCCCCCCCCCCCHHHHHHHCCEEEEEEEEEHHHCCEECEECCCCCCCCCCCCCEEEHHHH
RLVVKSFANMGTEKVRITGGEPTLRKDFLPIVETIAQNPTIKQIALTTNGYRMAKDVAAW
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
KEAGITSINVSIDSLDARMFHRITGIDKFEDVMRGLERAFEVGYQKIKVNSVLMRDLNDA
HHCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHEEHHHHHHHCCCCC
DFNQFLTWIKDKPIQMRFIELMQTGEMDHFFQQHHVSGQLLADKLINAGWTLQSKGLLDG
CHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCC
PAKVFKHPDYVGEVGLIMPYEKNFCASCNRLRVSAKGKLHLCLFGEEGITLRDLLQSDDQ
CHHHHCCCCCCCCCCEEECCCCCHHCCCCEEEEECCCEEEEEEECCCCCCHHHHHCCCCH
QLQLQARISSALQGKREHHFLHQGDSGVRANLASIGG
HHHHHHHHHHHHCCCHHHHHHHCCCCCCEEHHHHCCC
>Mature Secondary Structure
MQTIPIKHVGNDPLVDAFQRRYYYLRLSITDVCNFRCNYCLPNGYQPESNKPSFLTLNEI
CCCCCCCCCCCCHHHHHHHCCEEEEEEEEEHHHCCEECEECCCCCCCCCCCCCEEEHHHH
RLVVKSFANMGTEKVRITGGEPTLRKDFLPIVETIAQNPTIKQIALTTNGYRMAKDVAAW
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
KEAGITSINVSIDSLDARMFHRITGIDKFEDVMRGLERAFEVGYQKIKVNSVLMRDLNDA
HHCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHEEHHHHHHHCCCCC
DFNQFLTWIKDKPIQMRFIELMQTGEMDHFFQQHHVSGQLLADKLINAGWTLQSKGLLDG
CHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHCCCCEECCCCCCCC
PAKVFKHPDYVGEVGLIMPYEKNFCASCNRLRVSAKGKLHLCLFGEEGITLRDLLQSDDQ
CHHHHCCCCCCCCCCEEECCCCCHHCCCCEEEEECCCEEEEEEECCCCCCHHHHHCCCCH
QLQLQARISSALQGKREHHFLHQGDSGVRANLASIGG
HHHHHHHHHHHHCCCHHHHHHHCCCCCCEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100