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The map label for this gene is mltB [H]

Identifier: 15602378

GI number: 15602378

Start: 586981

End: 588075

Strand: Direct

Name: mltB [H]

Synonym: PM0513

Alternate gene names: 15602378

Gene position: 586981-588075 (Clockwise)

Preceding gene: 15602377

Following gene: 15602379

Centisome position: 26.0

GC content: 43.11

Gene sequence:

>1095_bases
GTGTCAATTAAATTATGGTTTCGTGTCAGTGGTTTGCTACTGAGCCTGATTCTGGTGGGGTGTGCAAATAAGCCACCGAT
TACACCGTTAGCCACAGGCAGTCAATTTTCTAAAGCACGTCAGGTGGAAAATTTTTCAGATTATGTCATTTTTTTAAAAC
GCAAAGCGCGTGCTGAAGGCGTCTCTGAACAAACGCTCAATGATCATAATGATATCCAGTATATTTCCAGAGCGATTGAG
CTTGATAGACAACAGGCAGGTCGAGTGCAAAAACGTGATCCTAATGCGCCTGTTCGTTCCAATCCACATGGCACAACCCA
TTATCTGAATCGGGTGTTAACACAACGTAAGGTGGATGTGGCGACCTCCCGTTATTGGGAACAACAAGCCCAATTGGAAA
AGGCAAGTCAACGTTTTGGTGTGCAAAAAGAATACCTCATGTCCTTATGGGGTATGGAAAGCAGTTTTGGGCATTATCAA
GGGCAATATGATGTATTGTCTGTATTGGCTACCTTAGCCTTTGAAGGACGCCGAGAAAGCTTGTTTAGCCGTGAATTTGT
TTCTGCCATGAAAATGTTAGAACGTCGTGATATCCCACGTCAAAAAATGTTAGGTTCATGGGCGGGAGCGATGGGACAAA
CTCAATTTATGCCAAGTTCTTATTTACGCTATGCCGCCGATGGTAATCACGACGGCGAAAAAGACATTTGGAAAAACCAC
GATGATGTGTTTGCCTCAATTGCCAGTTATTTGAGTACAGTGGGTTGGGATAAACAATTACCATGGGGGGTAGAAGTGCA
GTTGACCAAACCGCTCGATATCGCCTTGTCAGGTATTGAACATCAGAAAAAACGCCCTTTAACCGCTTGGCAACATTTAG
GCGTCGCACTCAAGTCAAATAATCAACAAAATCAAGAAAAACTAACCGCACTTGCTGGTGCAGATTTATGGCTTGTGCAA
CCTGATCGTGAAGTTGGACGTGCCTTTTTAGTGTCAAATAATTACCGCATATTACTCGATTGGAATCGCTCAAATTATTT
TGCGCTGAGCATTGGCATGTTTGCTGATCGCATTAAACATCAGTTAGGTTTGTAA

Upstream 100 bases:

>100_bases
TCGGGCTTGTTTACCTTTTTATTACGGGCATTACCACGCTTATTGCCCACTTCATTGTTACGTATCATTTATCAATTTTT
AAGAAAGTAGGAACGTTATT

Downstream 100 bases:

>100_bases
CGCGATTGATGTAAAACGATTGCTTCCCCGTCATGATTAGCGTTGCTGTATTGATTTCTGAGAGAAAAACAATGCATCAA
CGCGTTTCTTTTGCTAGAAT

Product: hypothetical protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSIKLWFRVSGLLLSLILVGCANKPPITPLATGSQFSKARQVENFSDYVIFLKRKARAEGVSEQTLNDHNDIQYISRAIE
LDRQQAGRVQKRDPNAPVRSNPHGTTHYLNRVLTQRKVDVATSRYWEQQAQLEKASQRFGVQKEYLMSLWGMESSFGHYQ
GQYDVLSVLATLAFEGRRESLFSREFVSAMKMLERRDIPRQKMLGSWAGAMGQTQFMPSSYLRYAADGNHDGEKDIWKNH
DDVFASIASYLSTVGWDKQLPWGVEVQLTKPLDIALSGIEHQKKRPLTAWQHLGVALKSNNQQNQEKLTALAGADLWLVQ
PDREVGRAFLVSNNYRILLDWNRSNYFALSIGMFADRIKHQLGL

Sequences:

>Translated_364_residues
MSIKLWFRVSGLLLSLILVGCANKPPITPLATGSQFSKARQVENFSDYVIFLKRKARAEGVSEQTLNDHNDIQYISRAIE
LDRQQAGRVQKRDPNAPVRSNPHGTTHYLNRVLTQRKVDVATSRYWEQQAQLEKASQRFGVQKEYLMSLWGMESSFGHYQ
GQYDVLSVLATLAFEGRRESLFSREFVSAMKMLERRDIPRQKMLGSWAGAMGQTQFMPSSYLRYAADGNHDGEKDIWKNH
DDVFASIASYLSTVGWDKQLPWGVEVQLTKPLDIALSGIEHQKKRPLTAWQHLGVALKSNNQQNQEKLTALAGADLWLVQ
PDREVGRAFLVSNNYRILLDWNRSNYFALSIGMFADRIKHQLGL
>Mature_363_residues
SIKLWFRVSGLLLSLILVGCANKPPITPLATGSQFSKARQVENFSDYVIFLKRKARAEGVSEQTLNDHNDIQYISRAIEL
DRQQAGRVQKRDPNAPVRSNPHGTTHYLNRVLTQRKVDVATSRYWEQQAQLEKASQRFGVQKEYLMSLWGMESSFGHYQG
QYDVLSVLATLAFEGRRESLFSREFVSAMKMLERRDIPRQKMLGSWAGAMGQTQFMPSSYLRYAADGNHDGEKDIWKNHD
DVFASIASYLSTVGWDKQLPWGVEVQLTKPLDIALSGIEHQKKRPLTAWQHLGVALKSNNQQNQEKLTALAGADLWLVQP
DREVGRAFLVSNNYRILLDWNRSNYFALSIGMFADRIKHQLGL

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=213, Percent_Identity=31.924882629108, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 41549; Mature: 41418

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLWFRVSGLLLSLILVGCANKPPITPLATGSQFSKARQVENFSDYVIFLKRKARAEG
CCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHEEHHHHHCC
VSEQTLNDHNDIQYISRAIELDRQQAGRVQKRDPNAPVRSNPHGTTHYLNRVLTQRKVDV
CCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ATSRYWEQQAQLEKASQRFGVQKEYLMSLWGMESSFGHYQGQYDVLSVLATLAFEGRRES
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHH
LFSREFVSAMKMLERRDIPRQKMLGSWAGAMGQTQFMPSSYLRYAADGNHDGEKDIWKNH
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHCCH
DDVFASIASYLSTVGWDKQLPWGVEVQLTKPLDIALSGIEHQKKRPLTAWQHLGVALKSN
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHCHHHHHCCCHHHHHHCCCCEECC
NQQNQEKLTALAGADLWLVQPDREVGRAFLVSNNYRILLDWNRSNYFALSIGMFADRIKH
CCHHHHHHHHHHCCCEEEECCCHHHCCEEEEECCEEEEEEECCCCEEEEEHHHHHHHHHH
QLGL
HHCC
>Mature Secondary Structure 
SIKLWFRVSGLLLSLILVGCANKPPITPLATGSQFSKARQVENFSDYVIFLKRKARAEG
CEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHEEHHHHHCC
VSEQTLNDHNDIQYISRAIELDRQQAGRVQKRDPNAPVRSNPHGTTHYLNRVLTQRKVDV
CCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
ATSRYWEQQAQLEKASQRFGVQKEYLMSLWGMESSFGHYQGQYDVLSVLATLAFEGRRES
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHH
LFSREFVSAMKMLERRDIPRQKMLGSWAGAMGQTQFMPSSYLRYAADGNHDGEKDIWKNH
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHCCH
DDVFASIASYLSTVGWDKQLPWGVEVQLTKPLDIALSGIEHQKKRPLTAWQHLGVALKSN
HHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHCHHHHHCCCHHHHHHCCCCEECC
NQQNQEKLTALAGADLWLVQPDREVGRAFLVSNNYRILLDWNRSNYFALSIGMFADRIKH
CCHHHHHHHHHHCCCEEEECCCHHHCCEEEEECCEEEEEEECCCCEEEEEHHHHHHHHHH
QLGL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]