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The map label for this gene is fdxI [H]

Identifier: 15602271

GI number: 15602271

Start: 474670

End: 475356

Strand: Reverse

Name: fdxI [H]

Synonym: PM0406

Alternate gene names: 15602271

Gene position: 475356-474670 (Counterclockwise)

Preceding gene: 15602272

Following gene: 15602270

Centisome position: 21.06

GC content: 42.36

Gene sequence:

>687_bases
ATGAGTAAGATTCAGATCAGTAACGATCGTAAAATCGTACGCCATAAATTGCCAGCTCGCCTTAGCCATTGGTTACTGGT
TATCTGTTTCTTTATTACCGGTTTATCGGGGCTGGCATTCTTCTTCCCGGATTTTTCCTGGTTAACGGAAATTCTAGGTA
CACCGCAACTGGCACGTTTTGTACACCCATTTACGGGGATTGTCATGTTTGTCGCGTTCCTGAATCTTTGCCGTATTTAC
TGGCGTCACAACTTCCCTGAGAAAAACGACTGGGTTTGGCTAAAAAATATTGGTGAAGTATTAAAAGGCAATGAGCATGC
GGTTTCTGATAATGGCAAATATAACCTTGGTCAGAAAATGTTGTTCTGGACCTTGATTCTTGCCATGATCACCTTACTTG
TAACCGGTATTATTATGTGGCGTCAATATTTCTCCCATAATTTCTCAATCCCGGTTATCCGTATTGCGATCTTGTTACAT
TCTACCAGTGCCTTCTTGTTATTCACTGGTATTATGGTACACATCTATATGGCATTCTGGGTCAAAGGGTCTATTCGTGG
TATGGTCGAAGGCTGGGTGACGGTTCGCTGGGCGAAAAAACACCACCCTCGTTGGTATCGTGAAGAAGTCCTACCTGAAC
TAGAAAAAGAAGAATTGGCAAAACAACAAAACCAAAGCCAACCATAA

Upstream 100 bases:

>100_bases
GGTATTATCCTCAGTGAAATTGCTCACTACATCACCATCGGTCCAAATACCGAAGAATTCGATGAACACGAGGAGCACGA
AGAGCATACAGGAGGCAATC

Downstream 100 bases:

>100_bases
ACACGACTCTGTGAATAAAAGTGCGGTCAAATTTTCAGAAATTTTTCTAAAAACGACCGCACTTTATTTATTCAAAAATA
AGGAAACATGATGAGTATCC

Product: FdxI

Products: CO2; NADH

Alternate protein names: Formate dehydrogenase subunit gamma; FDH subunit gamma [H]

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MSKIQISNDRKIVRHKLPARLSHWLLVICFFITGLSGLAFFFPDFSWLTEILGTPQLARFVHPFTGIVMFVAFLNLCRIY
WRHNFPEKNDWVWLKNIGEVLKGNEHAVSDNGKYNLGQKMLFWTLILAMITLLVTGIIMWRQYFSHNFSIPVIRIAILLH
STSAFLLFTGIMVHIYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELEKEELAKQQNQSQP

Sequences:

>Translated_228_residues
MSKIQISNDRKIVRHKLPARLSHWLLVICFFITGLSGLAFFFPDFSWLTEILGTPQLARFVHPFTGIVMFVAFLNLCRIY
WRHNFPEKNDWVWLKNIGEVLKGNEHAVSDNGKYNLGQKMLFWTLILAMITLLVTGIIMWRQYFSHNFSIPVIRIAILLH
STSAFLLFTGIMVHIYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELEKEELAKQQNQSQP
>Mature_227_residues
SKIQISNDRKIVRHKLPARLSHWLLVICFFITGLSGLAFFFPDFSWLTEILGTPQLARFVHPFTGIVMFVAFLNLCRIYW
RHNFPEKNDWVWLKNIGEVLKGNEHAVSDNGKYNLGQKMLFWTLILAMITLLVTGIIMWRQYFSHNFSIPVIRIAILLHS
TSAFLLFTGIMVHIYMAFWVKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELEKEELAKQQNQSQP

Specific function: Allows to use formate as major electron donor during anaerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787750, Length=221, Percent_Identity=52.0361990950226, Blast_Score=240, Evalue=7e-65,
Organism=Escherichia coli, GI1790325, Length=207, Percent_Identity=53.1400966183575, Blast_Score=236, Evalue=8e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 26844; Mature: 26713

Theoretical pI: Translated: 10.39; Mature: 10.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIQISNDRKIVRHKLPARLSHWLLVICFFITGLSGLAFFFPDFSWLTEILGTPQLARF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
VHPFTGIVMFVAFLNLCRIYWRHNFPEKNDWVWLKNIGEVLKGNEHAVSDNGKYNLGQKM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCEECCCCCCCHHHHH
LFWTLILAMITLLVTGIIMWRQYFSHNFSIPVIRIAILLHSTSAFLLFTGIMVHIYMAFW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELEKEELAKQQNQSQP
HHHHHHHHHHHHHHEEEHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKIQISNDRKIVRHKLPARLSHWLLVICFFITGLSGLAFFFPDFSWLTEILGTPQLARF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHH
VHPFTGIVMFVAFLNLCRIYWRHNFPEKNDWVWLKNIGEVLKGNEHAVSDNGKYNLGQKM
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHCCCCCEECCCCCCCHHHHH
LFWTLILAMITLLVTGIIMWRQYFSHNFSIPVIRIAILLHSTSAFLLFTGIMVHIYMAFW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VKGSIRGMVEGWVTVRWAKKHHPRWYREEVLPELEKEELAKQQNQSQP
HHHHHHHHHHHHHHEEEHHHCCCHHHHHHHCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]