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The map label for this gene is nth [H]

Identifier: 15602246

GI number: 15602246

Start: 450056

End: 450688

Strand: Reverse

Name: nth [H]

Synonym: PM0381

Alternate gene names: 15602246

Gene position: 450688-450056 (Counterclockwise)

Preceding gene: 15602247

Following gene: 15602245

Centisome position: 19.96

GC content: 41.71

Gene sequence:

>633_bases
ATGAATAAACAAAAACGAATTGAAATTTTAACACGCCTACGTGATCACAATCCGCATCCAACCACCGAACTCAATTACAG
TTCGCCTTTCGAGTTGTTAATTGCGGTGATCTTGTCTGCACAAGCCACCGATAAAGGCGTGAATAAAGCCACAGAAAAAT
TGTTTCCTGTCGCCAATACCCCACAAGCCATTTTAGATTTGGGGTTAGATGGACTAAAGGAATACATCAAAACCATTGGG
CTTTATAACAGTAAAGCCGAAAATATCATCAAAACGTGCCGAGATTTGATCGAAAAGCATAATGGTGAGATTCCTGAAAA
CCGTTCCGCGCTTGAAGCATTAGCGGGTGTGGGGAGGAAAACCGCCAATGTGGTGTTAAATACCGCATTTGGTCACCCGA
CTATCGCGGTAGATACCCATATTTTCCGCGTTTCTAATCGTACTGGCTTTGCGCCGGGAAAAGATGTGGTCAAAGTGGAA
GAAAAATTACTCAAAGTTGTACCTGATGAATTTAAAGTCGATGTGCACCATTGGTTGATTTTACATGGACGTTATACTTG
TATTGCTCGCAAGCCCCGTTGTGGATCTTGTCTCATTGAAGACCTTTGTGAGTTTAAAGAAAAAACAGAATAA

Upstream 100 bases:

>100_bases
TCGATAGCCATTTCTTGTTGGCGATTTTACCACCGGGCGCCTTTATTGGTTTAGGACTCATTTTAGCCATTAAAAATGTC
ATTGATCAGCGTAACAAAGC

Downstream 100 bases:

>100_bases
TTAAAAAATATTGGATGTAATTTTATGACAACAACAAAACAAGAAAGACAAACTTGGTCAAGTCGACTGACTTATATTAT
GACGGTTGCAGGGGCAACCG

Product: hypothetical protein

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNKQKRIEILTRLRDHNPHPTTELNYSSPFELLIAVILSAQATDKGVNKATEKLFPVANTPQAILDLGLDGLKEYIKTIG
LYNSKAENIIKTCRDLIEKHNGEIPENRSALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVE
EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCLIEDLCEFKEKTE

Sequences:

>Translated_210_residues
MNKQKRIEILTRLRDHNPHPTTELNYSSPFELLIAVILSAQATDKGVNKATEKLFPVANTPQAILDLGLDGLKEYIKTIG
LYNSKAENIIKTCRDLIEKHNGEIPENRSALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVE
EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCLIEDLCEFKEKTE
>Mature_210_residues
MNKQKRIEILTRLRDHNPHPTTELNYSSPFELLIAVILSAQATDKGVNKATEKLFPVANTPQAILDLGLDGLKEYIKTIG
LYNSKAENIIKTCRDLIEKHNGEIPENRSALEALAGVGRKTANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVE
EKLLKVVPDEFKVDVHHWLILHGRYTCIARKPRCGSCLIEDLCEFKEKTE

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=179, Percent_Identity=31.8435754189944, Blast_Score=91, Evalue=7e-19,
Organism=Escherichia coli, GI1787920, Length=210, Percent_Identity=82.8571428571429, Blast_Score=368, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=33.1428571428571, Blast_Score=100, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6324530, Length=187, Percent_Identity=26.2032085561497, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550361, Length=187, Percent_Identity=32.620320855615, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23519; Mature: 23519

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQKRIEILTRLRDHNPHPTTELNYSSPFELLIAVILSAQATDKGVNKATEKLFPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
PQAILDLGLDGLKEYIKTIGLYNSKAENIIKTCRDLIEKHNGEIPENRSALEALAGVGRK
HHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCH
TANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVEEKLLKVVPDEFKVDVHHWLI
HHHHEEECCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHEEHHHHEEE
LHGRYTCIARKPRCGSCLIEDLCEFKEKTE
EECCEEEEECCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKQKRIEILTRLRDHNPHPTTELNYSSPFELLIAVILSAQATDKGVNKATEKLFPVANT
CCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCC
PQAILDLGLDGLKEYIKTIGLYNSKAENIIKTCRDLIEKHNGEIPENRSALEALAGVGRK
HHHHHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCH
TANVVLNTAFGHPTIAVDTHIFRVSNRTGFAPGKDVVKVEEKLLKVVPDEFKVDVHHWLI
HHHHEEECCCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHHEEHHHHEEE
LHGRYTCIARKPRCGSCLIEDLCEFKEKTE
EECCEEEEECCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]