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The map label for this gene is murD

Identifier: 15602005

GI number: 15602005

Start: 165569

End: 166873

Strand: Direct

Name: murD

Synonym: PM0140

Alternate gene names: 15602005

Gene position: 165569-166873 (Clockwise)

Preceding gene: 15602004

Following gene: 15602006

Centisome position: 7.33

GC content: 42.99

Gene sequence:

>1305_bases
ATGCAAACGTCTTATAAAAATAAAAAAGTTACCGTGATTGGTTTGGGGAAAACAGGGCTATCTTGTGTTGATTTCCTACT
GGCAAAACAAGCGGATGTGCGTGTGATTGATACGAGAACGCAGCCTGCTGGGGCGGAACAATTGGCAAAAAATGTACCGT
TGCATACTGGCAGTTTAAACCAACAATGGTTACTCGAGAGTGATCTGATTATTATTAGTCCAGGATTAGCGGTCAAAACA
CCAGAAATTCAGACTGCACTTGCAGCAGGTATTGAGGTGATTGGCGATATTGAGCTGTTCTGCCGTGAAGCGAAAAAGCC
GATTATTGCGATTACGGGCTCAAATGGCAAAAGCACTGTGACATCACTTGTTGCGCACATGGTAAATGCGGCGGGCTTGA
AGGTTGGCATGGGCGGTAATATTGGTATTCCTGCACTGTCGTTATTAGAACAAGCACATGATATGTATGTATTGGAATTA
TCCAGTTTCCAACTAGAAACGACATACAGCTTAAAAGCCACTTCTGCAACGGTGTTAAATATTAGTGAAGATCACATGAA
TCGCTATGTGGACTTAGAAGACTATCGCCAAGCAAAATTAAAAATCTATCATCATGCCCAAACTGCGGTGATTAATGCGG
AAGATGCACTAACCGCAATGGACGGATTAAAAAATGGAGTAAGCTTTGGCGAAGACAATGCTGATTATTGGTTAAAAACC
GAAAAAGGGCGGTCATATTTGATGGCGAAAGACGAACGGGTGTTGGCTTGTGATGAGATGAAATTAGTTGGACGTCATAA
CTATATGAATGCACTGGCGGCCATCGCATTAGCACAGGCTGCCGGTATTCCATTAGAGAGTATTCGTCGTGCATTGCGTG
AGTTTAACGGGCTAGATCACCGTTTCCAATTAGCTCATTTTGCCCATGGTGTACGCTGGGTGAATGATTCAAAAGCCACC
AATGTGGGCAGTACTGTTGCCGCATTAACTGGCTTACAGTTAAACGGGACATTGCATTTGTTACTCGGTGGTGATGGTAA
AGGTGCGGACTTTTCGGAATTAGCCAGTTTAATTAATCAACCGAATATTATTTGCTATTGTTTTGGTCAAGATGGCGAAC
AACTTGCAGCACTCTCGCCAAGAAGTCAACGTTTTAGCACAATGGAAGAAGCGATCAACGCCTTACGCCCAACCTTGTCA
GCAGGTGATATGGTTTTACTTTCTCCTGCTTGTGCCAGTTTAGATCAGTTTAGCAGTTTCGAACAACGTGGTGATGAATT
TACACGTTTAGCAAAATTAAGTTAG

Upstream 100 bases:

>100_bases
AGAACCACGCGTGATCGTCCGCTTTTGGATTATTTCCTTAATGCTGGTCCTTGTTGGGCTCGTTACCTTAAAACTTCGCT
AAAACACAAAACGATATCTT

Downstream 100 bases:

>100_bases
GTGAAAGCAGTATGCCATTTTTAGATAAAGTAAAGCAGCAATATGAGGATTGGACGCGTATTACACCGAGTAATTTGTTA
TACGATCGTGCCTTGCTGTT

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MQTSYKNKKVTVIGLGKTGLSCVDFLLAKQADVRVIDTRTQPAGAEQLAKNVPLHTGSLNQQWLLESDLIIISPGLAVKT
PEIQTALAAGIEVIGDIELFCREAKKPIIAITGSNGKSTVTSLVAHMVNAAGLKVGMGGNIGIPALSLLEQAHDMYVLEL
SSFQLETTYSLKATSATVLNISEDHMNRYVDLEDYRQAKLKIYHHAQTAVINAEDALTAMDGLKNGVSFGEDNADYWLKT
EKGRSYLMAKDERVLACDEMKLVGRHNYMNALAAIALAQAAGIPLESIRRALREFNGLDHRFQLAHFAHGVRWVNDSKAT
NVGSTVAALTGLQLNGTLHLLLGGDGKGADFSELASLINQPNIICYCFGQDGEQLAALSPRSQRFSTMEEAINALRPTLS
AGDMVLLSPACASLDQFSSFEQRGDEFTRLAKLS

Sequences:

>Translated_434_residues
MQTSYKNKKVTVIGLGKTGLSCVDFLLAKQADVRVIDTRTQPAGAEQLAKNVPLHTGSLNQQWLLESDLIIISPGLAVKT
PEIQTALAAGIEVIGDIELFCREAKKPIIAITGSNGKSTVTSLVAHMVNAAGLKVGMGGNIGIPALSLLEQAHDMYVLEL
SSFQLETTYSLKATSATVLNISEDHMNRYVDLEDYRQAKLKIYHHAQTAVINAEDALTAMDGLKNGVSFGEDNADYWLKT
EKGRSYLMAKDERVLACDEMKLVGRHNYMNALAAIALAQAAGIPLESIRRALREFNGLDHRFQLAHFAHGVRWVNDSKAT
NVGSTVAALTGLQLNGTLHLLLGGDGKGADFSELASLINQPNIICYCFGQDGEQLAALSPRSQRFSTMEEAINALRPTLS
AGDMVLLSPACASLDQFSSFEQRGDEFTRLAKLS
>Mature_434_residues
MQTSYKNKKVTVIGLGKTGLSCVDFLLAKQADVRVIDTRTQPAGAEQLAKNVPLHTGSLNQQWLLESDLIIISPGLAVKT
PEIQTALAAGIEVIGDIELFCREAKKPIIAITGSNGKSTVTSLVAHMVNAAGLKVGMGGNIGIPALSLLEQAHDMYVLEL
SSFQLETTYSLKATSATVLNISEDHMNRYVDLEDYRQAKLKIYHHAQTAVINAEDALTAMDGLKNGVSFGEDNADYWLKT
EKGRSYLMAKDERVLACDEMKLVGRHNYMNALAAIALAQAAGIPLESIRRALREFNGLDHRFQLAHFAHGVRWVNDSKAT
NVGSTVAALTGLQLNGTLHLLLGGDGKGADFSELASLINQPNIICYCFGQDGEQLAALSPRSQRFSTMEEAINALRPTLS
AGDMVLLSPACASLDQFSSFEQRGDEFTRLAKLS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=432, Percent_Identity=59.9537037037037, Blast_Score=495, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_PASMU (Q9CPB0)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245077.1
- ProteinModelPortal:   Q9CPB0
- SMR:   Q9CPB0
- GeneID:   1243487
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0140
- NMPDR:   fig|272843.1.peg.140
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK03806
- BioCyc:   PMUL272843:PM0140-MONOMER
- BRENDA:   6.3.2.9
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 46935; Mature: 46935

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTSYKNKKVTVIGLGKTGLSCVDFLLAKQADVRVIDTRTQPAGAEQLAKNVPLHTGSLN
CCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCC
QQWLLESDLIIISPGLAVKTPEIQTALAAGIEVIGDIELFCREAKKPIIAITGSNGKSTV
CCEEEECCEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
TSLVAHMVNAAGLKVGMGGNIGIPALSLLEQAHDMYVLELSSFQLETTYSLKATSATVLN
HHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCEEEEEECCEEEEEEEEEEECCEEEEE
ISEDHMNRYVDLEDYRQAKLKIYHHAQTAVINAEDALTAMDGLKNGVSFGEDNADYWLKT
CCHHHHHHCCCHHHHHHHHEEEEEECEEEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEE
EKGRSYLMAKDERVLACDEMKLVGRHNYMNALAAIALAQAAGIPLESIRRALREFNGLDH
CCCCEEEEECCCEEEEECHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCH
RFQLAHFAHGVRWVNDSKATNVGSTVAALTGLQLNGTLHLLLGGDGKGADFSELASLINQ
HHHHHHHHCCEEEECCCCCCCCCHHHHHHCCEEECCEEEEEECCCCCCCCHHHHHHHHCC
PNIICYCFGQDGEQLAALSPRSQRFSTMEEAINALRPTLSAGDMVLLSPACASLDQFSSF
CCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHH
EQRGDEFTRLAKLS
HHCCHHHHHHHCCC
>Mature Secondary Structure
MQTSYKNKKVTVIGLGKTGLSCVDFLLAKQADVRVIDTRTQPAGAEQLAKNVPLHTGSLN
CCCCCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCC
QQWLLESDLIIISPGLAVKTPEIQTALAAGIEVIGDIELFCREAKKPIIAITGSNGKSTV
CCEEEECCEEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHH
TSLVAHMVNAAGLKVGMGGNIGIPALSLLEQAHDMYVLELSSFQLETTYSLKATSATVLN
HHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCEEEEEECCEEEEEEEEEEECCEEEEE
ISEDHMNRYVDLEDYRQAKLKIYHHAQTAVINAEDALTAMDGLKNGVSFGEDNADYWLKT
CCHHHHHHCCCHHHHHHHHEEEEEECEEEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEE
EKGRSYLMAKDERVLACDEMKLVGRHNYMNALAAIALAQAAGIPLESIRRALREFNGLDH
CCCCEEEEECCCEEEEECHHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCH
RFQLAHFAHGVRWVNDSKATNVGSTVAALTGLQLNGTLHLLLGGDGKGADFSELASLINQ
HHHHHHHHCCEEEECCCCCCCCCHHHHHHCCEEECCEEEEEECCCCCCCCHHHHHHHHCC
PNIICYCFGQDGEQLAALSPRSQRFSTMEEAINALRPTLSAGDMVLLSPACASLDQFSSF
CCEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHH
EQRGDEFTRLAKLS
HHCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100