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The map label for this gene is thrB

Identifier: 15601979

GI number: 15601979

Start: 139915

End: 140859

Strand: Direct

Name: thrB

Synonym: PM0114

Alternate gene names: 15601979

Gene position: 139915-140859 (Clockwise)

Preceding gene: 15601978

Following gene: 15601980

Centisome position: 6.2

GC content: 43.39

Gene sequence:

>945_bases
ATGCTCAGAATTTATGCACCAGCATCCAGTGCAAATTTAAGTGTTGGGTTTGATACCTTGGGGACCGCGGTGTCCCCGAT
TGATGGTTCTTTGCTGGGAGATGTGGTGCAAATTGAGGCACTGCCTAGCGGATTTGAACTAGAAAGTGCGGGATATTTTG
TGCGTAAATTGCCAAAAGAAGCGCAGAAAAATATTGTCTATCAAGCTTATGTGTTATTTAGTGAACGTTTAAAATTGCGT
AACATAGCTGTACGCCCATTGCGATTAACGTTAGAAAAAAATATGCCAATTGGTTCTGGCTTGGGGTCGAGTGCGTGTTC
TATCGTAGCGGCGTTGGTTGCACTCAATAAGTTTCATGCTGAACCTTTTTCTAAAATGGAACTGTTAGAAATGATGGGCG
AACTAGAAGGACGCATTTCGGGCTCAATCCATTATGATAATGTCGCACCTTGTTACTTAGGTGGGGTACAATTGATGGTG
CAATCTTTAGGTAATATTTGCCAACAATTGCCTTTTTTTGATGAGTGGTATTGGGTGTTGGCTTACCCCGGTATAGAAGT
CTCCACGGCAGAAGCCCGTGCGATTTTACCGAAAAGCTATACGCGTCAAGATGTGATTTCACAAGCGCGTCATTTAGGCA
GTTTTGTACATGCGTGTCATACTCGACAAGCGCAGCTTGCTGCGTTAATGATGAAGGACGTGATTGCGGAACCTTATCGT
GAAGCCTTGTTACCTAATTTTGCTGACGTGAAACAAGCCACACGAGATTTAGGGGCACTAGCAACGGGGATCTCGGGTTC
AGGACCAACAATATTCGCGATTGCGCCTGATTTAGGCACAGCGATGAAGTTATCCACTTATTTAGAAAACCATTATTTAC
AAAACAATGAAGGGTTTGTGCATATTTGTAAAGTTGATAATCAAGGTGCGAGAGCGCTCGATTAA

Upstream 100 bases:

>100_bases
TACGTGGTTATGGCGCGGGAAATTCCGTAACTGCAGCGGGGATTTTTGCCGATATATTGAGAACGCTACAACATTAATTG
GAAATGACATGGAAAATAAT

Downstream 100 bases:

>100_bases
TTGAATAACGGAAAAGCATATGAATTTATACAACATTAAACATCCAGAAGAACAAGTCAATTTTGCGCAAGCGGTACGTC
AAGGATTAGGCAAAAACCAA

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MLRIYAPASSANLSVGFDTLGTAVSPIDGSLLGDVVQIEALPSGFELESAGYFVRKLPKEAQKNIVYQAYVLFSERLKLR
NIAVRPLRLTLEKNMPIGSGLGSSACSIVAALVALNKFHAEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGVQLMV
QSLGNICQQLPFFDEWYWVLAYPGIEVSTAEARAILPKSYTRQDVISQARHLGSFVHACHTRQAQLAALMMKDVIAEPYR
EALLPNFADVKQATRDLGALATGISGSGPTIFAIAPDLGTAMKLSTYLENHYLQNNEGFVHICKVDNQGARALD

Sequences:

>Translated_314_residues
MLRIYAPASSANLSVGFDTLGTAVSPIDGSLLGDVVQIEALPSGFELESAGYFVRKLPKEAQKNIVYQAYVLFSERLKLR
NIAVRPLRLTLEKNMPIGSGLGSSACSIVAALVALNKFHAEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGVQLMV
QSLGNICQQLPFFDEWYWVLAYPGIEVSTAEARAILPKSYTRQDVISQARHLGSFVHACHTRQAQLAALMMKDVIAEPYR
EALLPNFADVKQATRDLGALATGISGSGPTIFAIAPDLGTAMKLSTYLENHYLQNNEGFVHICKVDNQGARALD
>Mature_314_residues
MLRIYAPASSANLSVGFDTLGTAVSPIDGSLLGDVVQIEALPSGFELESAGYFVRKLPKEAQKNIVYQAYVLFSERLKLR
NIAVRPLRLTLEKNMPIGSGLGSSACSIVAALVALNKFHAEPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGVQLMV
QSLGNICQQLPFFDEWYWVLAYPGIEVSTAEARAILPKSYTRQDVISQARHLGSFVHACHTRQAQLAALMMKDVIAEPYR
EALLPNFADVKQATRDLGALATGISGSGPTIFAIAPDLGTAMKLSTYLENHYLQNNEGFVHICKVDNQGARALD

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily

Homologues:

Organism=Escherichia coli, GI1786184, Length=314, Percent_Identity=61.1464968152866, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KHSE_PASMU (Q9CPD3)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245051.1
- ProteinModelPortal:   Q9CPD3
- SMR:   Q9CPD3
- PRIDE:   Q9CPD3
- GeneID:   1243461
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0114
- NMPDR:   fig|272843.1.peg.114
- HOGENOM:   HBG646290
- OMA:   GSAHADN
- ProtClustDB:   PRK01212
- BioCyc:   PMUL272843:PM0114-MONOMER
- BRENDA:   2.7.1.39
- GO:   GO:0005737
- HAMAP:   MF_00384
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PIRSF:   PIRSF000676
- PRINTS:   PR00958
- TIGRFAMs:   TIGR00191

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.39

Molecular weight: Translated: 34212; Mature: 34212

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIYAPASSANLSVGFDTLGTAVSPIDGSLLGDVVQIEALPSGFELESAGYFVRKLPKE
CEEEEECCCCCCEEECHHHHCCCCCCCCCHHHHHHEEEEECCCCCCCCCCCHHHHHHHHH
AQKNIVYQAYVLFSERLKLRNIAVRPLRLTLEKNMPIGSGLGSSACSIVAALVALNKFHA
HHHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCC
EPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGVQLMVQSLGNICQQLPFFDEWYWVL
CCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
AYPGIEVSTAEARAILPKSYTRQDVISQARHLGSFVHACHTRQAQLAALMMKDVIAEPYR
EECCCEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
EALLPNFADVKQATRDLGALATGISGSGPTIFAIAPDLGTAMKLSTYLENHYLQNNEGFV
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHEECCCCEE
HICKVDNQGARALD
EEEEECCCCCCCCC
>Mature Secondary Structure
MLRIYAPASSANLSVGFDTLGTAVSPIDGSLLGDVVQIEALPSGFELESAGYFVRKLPKE
CEEEEECCCCCCEEECHHHHCCCCCCCCCHHHHHHEEEEECCCCCCCCCCCHHHHHHHHH
AQKNIVYQAYVLFSERLKLRNIAVRPLRLTLEKNMPIGSGLGSSACSIVAALVALNKFHA
HHHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCC
EPFSKMELLEMMGELEGRISGSIHYDNVAPCYLGGVQLMVQSLGNICQQLPFFDEWYWVL
CCHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
AYPGIEVSTAEARAILPKSYTRQDVISQARHLGSFVHACHTRQAQLAALMMKDVIAEPYR
EECCCEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
EALLPNFADVKQATRDLGALATGISGSGPTIFAIAPDLGTAMKLSTYLENHYLQNNEGFV
HHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHEECCCCEE
HICKVDNQGARALD
EEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100