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The map label for this gene is rnhA

Identifier: 15601972

GI number: 15601972

Start: 132840

End: 133304

Strand: Direct

Name: rnhA

Synonym: PM0107

Alternate gene names: 15601972

Gene position: 132840-133304 (Clockwise)

Preceding gene: 15601969

Following gene: 15601978

Centisome position: 5.88

GC content: 41.72

Gene sequence:

>465_bases
ATGCAAAAACAAATTGAAATTTTTACGGATGGTTCCTGTTTAGGCAATCCCGGACCGGGTGGGATCGGCGTATTGCTACG
TTATAAACAACATGAAAAACAGATTTCAGCAGGTTATTTTTTAACAACTAATAACCGTATGGAATTGCGTGCGGTGATTG
AAGCCTTAAATACTCTCAAAGAGCCTTGCTCAGTGACGTTACACAGTGATAGCCAATATATGAAAAATGGCATCACCAAA
TGGATCTTTAATTGGAAAAAAAATAACTGGAAAGCCAGCACCGGCAAGCCCGTTAAGAACCAAGATTTATGGATACAGCT
CGATCAAGCGATCCAACGTCACCACATCAATTGGCAATGGGTCAAAGGGCATTCTGGACACATTGAAAATGAAATCTGCG
ATCAACTCGCTAAAGCGGGCGCAGAAAATCCTACGTTGCAAGATGTGGGCTATCAGCCTGAATAA

Upstream 100 bases:

>100_bases
ATTTGGCGAGTCGGTTGATTCACTGGGGTCATTTTCTTTCTATCTCTTTCAGTTAATTTCGGTTATGATCTTGGCTTAAT
GAGGAATTGTAACAAAGATT

Downstream 100 bases:

>100_bases
AACGTTTTCAAAAATGACCGCACTTTTCGGTTATTTTTCTTGTCAAAAAACGCACGTTCCCCACTAAAACAAACTCTGCT
GCCCGGTTAAAAACTCGGGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase HI

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MQKQIEIFTDGSCLGNPGPGGIGVLLRYKQHEKQISAGYFLTTNNRMELRAVIEALNTLKEPCSVTLHSDSQYMKNGITK
WIFNWKKNNWKASTGKPVKNQDLWIQLDQAIQRHHINWQWVKGHSGHIENEICDQLAKAGAENPTLQDVGYQPE

Sequences:

>Translated_154_residues
MQKQIEIFTDGSCLGNPGPGGIGVLLRYKQHEKQISAGYFLTTNNRMELRAVIEALNTLKEPCSVTLHSDSQYMKNGITK
WIFNWKKNNWKASTGKPVKNQDLWIQLDQAIQRHHINWQWVKGHSGHIENEICDQLAKAGAENPTLQDVGYQPE
>Mature_154_residues
MQKQIEIFTDGSCLGNPGPGGIGVLLRYKQHEKQISAGYFLTTNNRMELRAVIEALNTLKEPCSVTLHSDSQYMKNGITK
WIFNWKKNNWKASTGKPVKNQDLWIQLDQAIQRHHINWQWVKGHSGHIENEICDQLAKAGAENPTLQDVGYQPE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=150, Percent_Identity=37.3333333333333, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1786408, Length=154, Percent_Identity=65.5844155844156, Blast_Score=217, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI115532542, Length=141, Percent_Identity=34.0425531914894, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17505482, Length=132, Percent_Identity=39.3939393939394, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17137476, Length=142, Percent_Identity=34.5070422535211, Blast_Score=83, Evalue=7e-17,
Organism=Drosophila melanogaster, GI45552509, Length=142, Percent_Identity=34.5070422535211, Blast_Score=83, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_PASMU (P57813)

Other databases:

- EMBL:   AE004439
- RefSeq:   NP_245044.1
- ProteinModelPortal:   P57813
- SMR:   P57813
- GeneID:   1243454
- GenomeReviews:   AE004439_GR
- KEGG:   pmu:PM0107
- NMPDR:   fig|272843.1.peg.107
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   PMUL272843:PM0107-MONOMER
- BRENDA:   3.1.26.4
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17556; Mature: 17556

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKQIEIFTDGSCLGNPGPGGIGVLLRYKQHEKQISAGYFLTTNNRMELRAVIEALNTLK
CCCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHC
EPCSVTLHSDSQYMKNGITKWIFNWKKNNWKASTGKPVKNQDLWIQLDQAIQRHHINWQW
CCCEEEEECCHHHHHCCHHEEEEECCCCCEECCCCCCCCCCCCEEEHHHHHHHHCCCEEE
VKGHSGHIENEICDQLAKAGAENPTLQDVGYQPE
ECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKQIEIFTDGSCLGNPGPGGIGVLLRYKQHEKQISAGYFLTTNNRMELRAVIEALNTLK
CCCEEEEEECCCCCCCCCCCCEEEEEEEHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHC
EPCSVTLHSDSQYMKNGITKWIFNWKKNNWKASTGKPVKNQDLWIQLDQAIQRHHINWQW
CCCEEEEECCHHHHHCCHHEEEEECCCCCEECCCCCCCCCCCCEEEHHHHHHHHCCCEEE
VKGHSGHIENEICDQLAKAGAENPTLQDVGYQPE
ECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100