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The map label for this gene is pflA [H]

Identifier: 15601942

GI number: 15601942

Start: 105123

End: 105863

Strand: Direct

Name: pflA [H]

Synonym: PM0077

Alternate gene names: 15601942

Gene position: 105123-105863 (Clockwise)

Preceding gene: 15601941

Following gene: 15601949

Centisome position: 4.66

GC content: 39.54

Gene sequence:

>741_bases
ATGGCTGTTGTGGGAAGAATTCATTCTTACGAATCCTGTGGCACTGTTGATGGGCCTGGTATTCGTTTTATTTTATTTAT
GCAAGGTTGCTTAATGCGTTGTCAATACTGTCACAATCGAGATACGTGGGATTTGCACGCCGGCAAGGAAATTACTGTTG
AGGAATTAATGAAAGAAGTGGTGACTTATCGCCACTTTATGAATGCATCTGGTGGAGGCGTTACCGCCTCTGGTGGCGAA
GCGGTATTGCAAGCAGAGTTTGTACGTGATTGGTTTAGCGCTTGTAAAAAAGAGGGGATTCATACCTGTTTAGATACCAA
CGGTTTTGTGCGTAATTATGATCACGTGATTGATGAGCTGTTAGATGTGACGGATCTTGTATTACTCGATCTCAAACAAC
TGAATGATAAAATTCACCAAAACTTAATCGGTGTACCAAATAAGCGCACTTTAGAGTTTGCTCAATATCTAGCCAAACGT
AATCAACCTGTTTGGATTCGTTATGTTGTCGTACCGGGTTATACCGATGCCGATGAAGATATTCATTTATTGGGTCACTT
TATTAAAGACATGAAAAACATTGAGAAAGTCGAATTATTGCCTTATCACCGTTTGGGCGCGCACAAATGGGAAGCAATGG
GTGAAAAATATGAACTGGAAGAGGTCAATCCTCCAACAAAAGAATCCTTAGAACATATTAAATCGATTCTGGAAAGTTAT
GGGCATATTATAAAATATTAA

Upstream 100 bases:

>100_bases
GTATTTTTTTGTTGTGAGGACAATGTAATATAGTCGAATAATTCGCAACAATACGGCTAAATTTCGATTATAATAAGCAA
AAAATGTTTAAGGATTGATT

Downstream 100 bases:

>100_bases
TTTAGCTTGCTAGGCGATGTAAGAAAGAGGAGCAGAATATTTCTGCTCTTTTTTATTTACACACAATTTCATCTGAAACG
AATACACTTTGCCCAATTAC

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAVVGRIHSYESCGTVDGPGIRFILFMQGCLMRCQYCHNRDTWDLHAGKEITVEELMKEVVTYRHFMNASGGGVTASGGE
AVLQAEFVRDWFSACKKEGIHTCLDTNGFVRNYDHVIDELLDVTDLVLLDLKQLNDKIHQNLIGVPNKRTLEFAQYLAKR
NQPVWIRYVVVPGYTDADEDIHLLGHFIKDMKNIEKVELLPYHRLGAHKWEAMGEKYELEEVNPPTKESLEHIKSILESY
GHIIKY

Sequences:

>Translated_246_residues
MAVVGRIHSYESCGTVDGPGIRFILFMQGCLMRCQYCHNRDTWDLHAGKEITVEELMKEVVTYRHFMNASGGGVTASGGE
AVLQAEFVRDWFSACKKEGIHTCLDTNGFVRNYDHVIDELLDVTDLVLLDLKQLNDKIHQNLIGVPNKRTLEFAQYLAKR
NQPVWIRYVVVPGYTDADEDIHLLGHFIKDMKNIEKVELLPYHRLGAHKWEAMGEKYELEEVNPPTKESLEHIKSILESY
GHIIKY
>Mature_245_residues
AVVGRIHSYESCGTVDGPGIRFILFMQGCLMRCQYCHNRDTWDLHAGKEITVEELMKEVVTYRHFMNASGGGVTASGGEA
VLQAEFVRDWFSACKKEGIHTCLDTNGFVRNYDHVIDELLDVTDLVLLDLKQLNDKIHQNLIGVPNKRTLEFAQYLAKRN
QPVWIRYVVVPGYTDADEDIHLLGHFIKDMKNIEKVELLPYHRLGAHKWEAMGEKYELEEVNPPTKESLEHIKSILESYG
HIIKY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=76.4227642276423, Blast_Score=413, Evalue=1e-117,
Organism=Escherichia coli, GI1790389, Length=271, Percent_Identity=26.9372693726937, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790839, Length=272, Percent_Identity=29.4117647058824, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 28235; Mature: 28104

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVGRIHSYESCGTVDGPGIRFILFMQGCLMRCQYCHNRDTWDLHAGKEITVEELMKEV
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHH
VTYRHFMNASGGGVTASGGEAVLQAEFVRDWFSACKKEGIHTCLDTNGFVRNYDHVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LDVTDLVLLDLKQLNDKIHQNLIGVPNKRTLEFAQYLAKRNQPVWIRYVVVPGYTDADED
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHH
IHLLGHFIKDMKNIEKVELLPYHRLGAHKWEAMGEKYELEEVNPPTKESLEHIKSILESY
HHHHHHHHHHHHCHHHHHHCCCHHHCCHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHH
GHIIKY
HHHHCC
>Mature Secondary Structure 
AVVGRIHSYESCGTVDGPGIRFILFMQGCLMRCQYCHNRDTWDLHAGKEITVEELMKEV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHH
VTYRHFMNASGGGVTASGGEAVLQAEFVRDWFSACKKEGIHTCLDTNGFVRNYDHVIDEL
HHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LDVTDLVLLDLKQLNDKIHQNLIGVPNKRTLEFAQYLAKRNQPVWIRYVVVPGYTDADED
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHH
IHLLGHFIKDMKNIEKVELLPYHRLGAHKWEAMGEKYELEEVNPPTKESLEHIKSILESY
HHHHHHHHHHHHCHHHHHHCCCHHHCCHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHH
GHIIKY
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]