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The map label for this gene is pflB [H]

Identifier: 15601940

GI number: 15601940

Start: 100417

End: 102747

Strand: Direct

Name: pflB [H]

Synonym: PM0075

Alternate gene names: 15601940

Gene position: 100417-102747 (Clockwise)

Preceding gene: 15601939

Following gene: 15601941

Centisome position: 4.45

GC content: 41.83

Gene sequence:

>2331_bases
TTGAGTATGAGTCAATTAAATGAAACTCAACAAAAGGCTTGGGAAGGGTTTACCGGTGGAGACTGGCAAACAGAAGTGAA
TGTACGCGATTTTATTCAAAAAAACTATACACCTTATGAAGGGGATGAGTCTTTCTTAGCCGATGCCACAGAAGCCACAA
CAAAATTGTGGAATGATGTGATGGAAAAAATCAAAGTGGAAAATAAAACCCACGAGCCGTATGACATTGATTGCGATACG
CCGTCAACAATCACTTCACATGCGCCGGGTTATATTGATAAAAGCTTAGAGAAAATTGTTGGCTTGCAAACAGATGCACC
TTTAAAACGTGCGATTATGCCGTTTGGTGGTATCAACATGGTGAAAGGTTCTTGTAAAGTTTATCGCCGTGAACTAAAAC
CAGAAGTCGAGCAAATCTTTACTGAATACCGTAAAACCCACAACCAAGGTGTATTTGATGTTTATACTCCCGATATTTTA
CGTTGCCGTAAATCTGGGGTAATCACAGGGCTTCCGGATGCTTATGGTCGTGGTCGTATTATTGGTGACTATCGTCGTAT
GGCTCTGTATGGTGCAGACTTCTTAATGAAAGATAAATTTAACCAATTTACGTCATTACAAGACAAACTAGAGCGTGGCG
AAGATATCCAAGCAACGATTCAATTACGTGAAGAAATTGCGGAACAACACCGTGCTTTAGGCAAAATGAAAGAAATGGCG
GCTTCTTACGGTTACGATATTTCGGGTCCAGCGACTAACGCACATGAAGCGGTACAATGGACTTACTTTGCTTATCTTGC
TGCAGTGAAATCGCAAAACGGTGCCGCCATGTCATTTGGTCGTGTATCGACTTTCTTGGATATTTATATTGAGCGTGATT
TAAAAGCAGGCAAAATTACAGAACAAGAAGCACAAGAATTAATTGACCACTTAGTCATGAAATTACGTATGGTGCGTTTC
TTACGTACCCCAGAGTACGATCAATTATTCTCGGGCGACCCAATGTGGGCAACGGAAACCTTAGCGGGTATGGGCTTAGA
TGGTCGTACTTTAGTAACCAAAAACAGCTTCCGTATCTTACACACCCTTTATACGATGGGACCGTCACCAGAGCCAAACT
TAACGATTCTTTGGTCTGAAAAATTACCAGAAGGTTTCAAACGTTATGCGGCAAAAGTGTCTATCGATACGTCTTCTGTT
CAGTATGAAAACGATGACTTAATGCGTCCTGACTTCCAAAACGATGACTATGCGATTGCATGCTGCGTGTCGCCAATGAT
CGTGGGTAAAATGATGCAATTCTTCGGTGCACGTGCAAACTTAGCGAAAACCTTGTTATACGCAATCAATGGCGGTGTGG
ATGAGAAATCAGGTGACCAAGTAGGACCGAAAACCGATCCAATTACCAGCGAATACTTAGATTATGATGATGTCATGACG
CGCTTAGACAGCTTTATGGATTGGTTAGCGAAACAATATGTTACTGCATTGAACATCATTCACTTTATGCACGATAAATA
CGCGTATGAAGCCGCACTAATGGCGCTTCATGATCGTGATGTATTCCGTACTATGGCTTGTGGTATCGCAGGGCTTTCTG
TCGCAGCGGACTCTTTATCTGCCATTAAATATGCGAAAGTGAAACCAGTTCGTGGTGATATCGAAATCAAAAATAAAGCG
GGTGAAGTGGTTGGTATTGCAAAAGATGTGGCAATCGACTTTGAAATTGAAGGCGAATATCCACAATTCGGTAACAACGA
TAACCGTGTTGATGAAATCGCTTGTGATTTAGTTGAACGCTTTATGAAGAAAATCCAAAAATTGGGTACTTATCGTAATG
CGACACCGACACAATCTGTACTTACTATTACATCTAACGTGGTTTATGGTAAGAAAACAGGTAATACCCCAGATGGTCGT
CGTTCTGGTGCACCATTTGGACCGGGTGCGAACCCAATGCACGGTCGTGACCAAAAAGGTGCGGTGGCATCGTTAACTTC
TGTTGCGAAATTGCCATTTGCTTATGCGAAAGATGGTATTTCTTATACCTTCTCAATCGTACCAAATGCCTTAGGTAAAG
ATTACGAAGCACAAAAACGTAACCTTGCAGGTCTAATGGATGGTTACTTCCACCATGAAGCAACGATTGAAGGCGGACAA
CACTTAAACGTGAACGTCATGAACCGTGAAATGTTGTTAGATGCAATGGAAAATCCGGAGAAATATCCACAATTAACCAT
TCGTGTTTCGGGTTATGCAGTACGCTTTAACTCGTTAACCAAAGAACAACAACAAGACGTGATTACGCGTACCTTTACTC
AAGCGATGTAA

Upstream 100 bases:

>100_bases
TACATTAACCGCCCACATTAAGCAGTTTAGGTGTAAAAGTAACAATGTTACTTTTATGTAAGCGATGACAAGATTTATTT
TCATTAATTAATAGAAGGTA

Downstream 100 bases:

>100_bases
TTGAGTAGGTGAATGGACATAAAAGCCTCTGTGTGAAAGCGCAGAGGCTTTTTTATGTGATTAAACATTGACTTCTGTTC
ATACCAGTAGTGGTCTGGCT

Product: PflB

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 776; Mature: 775

Protein sequence:

>776_residues
MSMSQLNETQQKAWEGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLADATEATTKLWNDVMEKIKVENKTHEPYDIDCDT
PSTITSHAPGYIDKSLEKIVGLQTDAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDIL
RCRKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKFNQFTSLQDKLERGEDIQATIQLREEIAEQHRALGKMKEMA
ASYGYDISGPATNAHEAVQWTYFAYLAAVKSQNGAAMSFGRVSTFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRF
LRTPEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEKLPEGFKRYAAKVSIDTSSV
QYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGGVDEKSGDQVGPKTDPITSEYLDYDDVMT
RLDSFMDWLAKQYVTALNIIHFMHDKYAYEAALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIEIKNKA
GEVVGIAKDVAIDFEIEGEYPQFGNNDNRVDEIACDLVERFMKKIQKLGTYRNATPTQSVLTITSNVVYGKKTGNTPDGR
RSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATIEGGQ
HLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQAM

Sequences:

>Translated_776_residues
MSMSQLNETQQKAWEGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLADATEATTKLWNDVMEKIKVENKTHEPYDIDCDT
PSTITSHAPGYIDKSLEKIVGLQTDAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDIL
RCRKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKFNQFTSLQDKLERGEDIQATIQLREEIAEQHRALGKMKEMA
ASYGYDISGPATNAHEAVQWTYFAYLAAVKSQNGAAMSFGRVSTFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRF
LRTPEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEKLPEGFKRYAAKVSIDTSSV
QYENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGGVDEKSGDQVGPKTDPITSEYLDYDDVMT
RLDSFMDWLAKQYVTALNIIHFMHDKYAYEAALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIEIKNKA
GEVVGIAKDVAIDFEIEGEYPQFGNNDNRVDEIACDLVERFMKKIQKLGTYRNATPTQSVLTITSNVVYGKKTGNTPDGR
RSGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATIEGGQ
HLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQAM
>Mature_775_residues
SMSQLNETQQKAWEGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLADATEATTKLWNDVMEKIKVENKTHEPYDIDCDTP
STITSHAPGYIDKSLEKIVGLQTDAPLKRAIMPFGGINMVKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILR
CRKSGVITGLPDAYGRGRIIGDYRRMALYGADFLMKDKFNQFTSLQDKLERGEDIQATIQLREEIAEQHRALGKMKEMAA
SYGYDISGPATNAHEAVQWTYFAYLAAVKSQNGAAMSFGRVSTFLDIYIERDLKAGKITEQEAQELIDHLVMKLRMVRFL
RTPEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRILHTLYTMGPSPEPNLTILWSEKLPEGFKRYAAKVSIDTSSVQ
YENDDLMRPDFQNDDYAIACCVSPMIVGKMMQFFGARANLAKTLLYAINGGVDEKSGDQVGPKTDPITSEYLDYDDVMTR
LDSFMDWLAKQYVTALNIIHFMHDKYAYEAALMALHDRDVFRTMACGIAGLSVAADSLSAIKYAKVKPVRGDIEIKNKAG
EVVGIAKDVAIDFEIEGEYPQFGNNDNRVDEIACDLVERFMKKIQKLGTYRNATPTQSVLTITSNVVYGKKTGNTPDGRR
SGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATIEGGQH
LNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQAM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=774, Percent_Identity=82.9457364341085, Blast_Score=1359, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=764, Percent_Identity=74.738219895288, Blast_Score=1226, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=567, Percent_Identity=26.63139329806, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1790388, Length=698, Percent_Identity=23.6389684813754, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI1788933, Length=64, Percent_Identity=75, Blast_Score=106, Evalue=5e-24,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 87127; Mature: 86996

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSQLNETQQKAWEGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLADATEATTKLWNDV
CCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
MEKIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQTDAPLKRAIMPFGGINM
HHHHHHCCCCCCCEECCCCCCCCHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCHH
VKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRCRKSGVITGLPDAYGRGRI
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEECCCCCCCCCCE
IGDYRRMALYGADFLMKDKFNQFTSLQDKLERGEDIQATIQLREEIAEQHRALGKMKEMA
EHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ASYGYDISGPATNAHEAVQWTYFAYLAAVKSQNGAAMSFGRVSTFLDIYIERDLKAGKIT
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCCC
EQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRIL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCCCCEEEECCCHHHH
HTLYTMGPSPEPNLTILWSEKLPEGFKRYAAKVSIDTSSVQYENDDLMRPDFQNDDYAIA
HHHHHCCCCCCCCEEEEECCCCCHHHHHHHHEEEEECCCEEECCCCCCCCCCCCCCCEEE
CCVSPMIVGKMMQFFGARANLAKTLLYAINGGVDEKSGDQVGPKTDPITSEYLDYDDVMT
EHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHH
RLDSFMDWLAKQYVTALNIIHFMHDKYAYEAALMALHDRDVFRTMACGIAGLSVAADSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKYAKVKPVRGDIEIKNKAGEVVGIAKDVAIDFEIEGEYPQFGNNDNRVDEIACDLVER
HHHHHEECCCCCCEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
FMKKIQKLGTYRNATPTQSVLTITSNVVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKG
HHHHHHHHCCCCCCCCCHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATIEGGQ
HHHHHHHHHHCCHHHHHCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCEEECCCC
HLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQAM
EEEEEECHHHHHHHHHHCCCCCCEEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SMSQLNETQQKAWEGFTGGDWQTEVNVRDFIQKNYTPYEGDESFLADATEATTKLWNDV
CHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
MEKIKVENKTHEPYDIDCDTPSTITSHAPGYIDKSLEKIVGLQTDAPLKRAIMPFGGINM
HHHHHHCCCCCCCEECCCCCCCCHHCCCCCHHHHHHHHHHCCCCCCCHHHHHCCCCCCHH
VKGSCKVYRRELKPEVEQIFTEYRKTHNQGVFDVYTPDILRCRKSGVITGLPDAYGRGRI
HHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEECCCCCCCCCCE
IGDYRRMALYGADFLMKDKFNQFTSLQDKLERGEDIQATIQLREEIAEQHRALGKMKEMA
EHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ASYGYDISGPATNAHEAVQWTYFAYLAAVKSQNGAAMSFGRVSTFLDIYIERDLKAGKIT
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHCCCCCCCCC
EQEAQELIDHLVMKLRMVRFLRTPEYDQLFSGDPMWATETLAGMGLDGRTLVTKNSFRIL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHCCCCCCCEEEECCCHHHH
HTLYTMGPSPEPNLTILWSEKLPEGFKRYAAKVSIDTSSVQYENDDLMRPDFQNDDYAIA
HHHHHCCCCCCCCEEEEECCCCCHHHHHHHHEEEEECCCEEECCCCCCCCCCCCCCCEEE
CCVSPMIVGKMMQFFGARANLAKTLLYAINGGVDEKSGDQVGPKTDPITSEYLDYDDVMT
EHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHH
RLDSFMDWLAKQYVTALNIIHFMHDKYAYEAALMALHDRDVFRTMACGIAGLSVAADSLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIKYAKVKPVRGDIEIKNKAGEVVGIAKDVAIDFEIEGEYPQFGNNDNRVDEIACDLVER
HHHHHEECCCCCCEEEECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHH
FMKKIQKLGTYRNATPTQSVLTITSNVVYGKKTGNTPDGRRSGAPFGPGANPMHGRDQKG
HHHHHHHHCCCCCCCCCHHHEEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDYEAQKRNLAGLMDGYFHHEATIEGGQ
HHHHHHHHHHCCHHHHHCCCCEEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCEEECCCC
HLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRFNSLTKEQQQDVITRTFTQAM
EEEEEECHHHHHHHHHHCCCCCCEEEEEEECEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]