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The map label for this gene is ksgA [H]

Identifier: 15594935

GI number: 15594935

Start: 609061

End: 609906

Strand: Reverse

Name: ksgA [H]

Synonym: BB0590

Alternate gene names: 15594935

Gene position: 609906-609061 (Counterclockwise)

Preceding gene: 15594936

Following gene: 15594932

Centisome position: 66.97

GC content: 24.94

Gene sequence:

>846_bases
ATGATACTATCTCTTTTATCTATGAATATCAACTATAACAGTATAACTAGCATAAAACAAACATTAAAAGAAAGAAAAAT
CGCTCCAAGAAAATTATGGGGACAAAACTATTTAATCAACGAAAGCATAAGGCAAAAAATAATAGAAAGCTTAGATATAA
AAGAAAATGAAAAAATCTGGGAAATTGGCCCAGGCCTTGGCGCAATGACTGAGATTTTATTAAAAAAAACTAATCTTTTA
ACCGCATTTGAAATTGACTTAAAATATTCAGAAATATTAAATGAAAAATTTGGAAAATTAAAAAACTTTAAATTGATAAA
AGGGGATTTTTTAAAAAAATACAAAAACGAAAATCAAAACATTGATAAAATATTTTCAAATTTGCCATACAATATTGCAT
CAAAAGTAATATCTAAATTAATTGAAGAAAACTTTTTAAAAGAAATGGTATTCACAGTGCAAAAAGAATTGGCCGACAGA
ATAACTGCAAAAATAAACAGCAAAAACTATTCTTCATTTACGGTCTTAGTACAATCACACTTCAAGGTAATTAAAATATT
AGACATAGGAGAAAACAATTTTTATCCTGCACCTAAGGTTAAGTCCACAACACTAAAATTAATTCCTAAAAAAAACAACA
TAAAAAACTTCAAAGAATTCAATAAATTGGTTAGAACTGTATTTTCAAACAGAAGAAAGAAATTAAAAAACACTATTATT
AATTTCATTACCAATAAAGCTACTCTGAGAGAAAATTTTTTAAAAGAATATTTAGACAAAAGACCTGAAAACATTTCTGT
TGAAGAATTTATACAAATTTCCAACACTTTAAATGCTTATCATTAA

Upstream 100 bases:

>100_bases
TTTAATATTAAGTATTTTTCTTATAACTTACATTATTTATAATAACGAAACTAAAAAAAATTAAAATAATTTTAAAATAT
CTGTCATTATTAAGAATAAT

Downstream 100 bases:

>100_bases
AGCACTTGCAAATACAATTTCATCAACAGAACAGCCCCTTGAAAGATCGCTAATGGGCTTGGAAAAACCTTGTAAAAAGG
GACCGTAGGCCTTGGCAAAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MILSLLSMNINYNSITSIKQTLKERKIAPRKLWGQNYLINESIRQKIIESLDIKENEKIWEIGPGLGAMTEILLKKTNLL
TAFEIDLKYSEILNEKFGKLKNFKLIKGDFLKKYKNENQNIDKIFSNLPYNIASKVISKLIEENFLKEMVFTVQKELADR
ITAKINSKNYSSFTVLVQSHFKVIKILDIGENNFYPAPKVKSTTLKLIPKKNNIKNFKEFNKLVRTVFSNRRKKLKNTII
NFITNKATLRENFLKEYLDKRPENISVEEFIQISNTLNAYH

Sequences:

>Translated_281_residues
MILSLLSMNINYNSITSIKQTLKERKIAPRKLWGQNYLINESIRQKIIESLDIKENEKIWEIGPGLGAMTEILLKKTNLL
TAFEIDLKYSEILNEKFGKLKNFKLIKGDFLKKYKNENQNIDKIFSNLPYNIASKVISKLIEENFLKEMVFTVQKELADR
ITAKINSKNYSSFTVLVQSHFKVIKILDIGENNFYPAPKVKSTTLKLIPKKNNIKNFKEFNKLVRTVFSNRRKKLKNTII
NFITNKATLRENFLKEYLDKRPENISVEEFIQISNTLNAYH
>Mature_281_residues
MILSLLSMNINYNSITSIKQTLKERKIAPRKLWGQNYLINESIRQKIIESLDIKENEKIWEIGPGLGAMTEILLKKTNLL
TAFEIDLKYSEILNEKFGKLKNFKLIKGDFLKKYKNENQNIDKIFSNLPYNIASKVISKLIEENFLKEMVFTVQKELADR
ITAKINSKNYSSFTVLVQSHFKVIKILDIGENNFYPAPKVKSTTLKLIPKKNNIKNFKEFNKLVRTVFSNRRKKLKNTII
NFITNKATLRENFLKEYLDKRPENISVEEFIQISNTLNAYH

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits [H]

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657198, Length=276, Percent_Identity=28.2608695652174, Blast_Score=105, Evalue=6e-23,
Organism=Homo sapiens, GI156415992, Length=249, Percent_Identity=26.1044176706827, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786236, Length=266, Percent_Identity=27.8195488721804, Blast_Score=124, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI25146882, Length=207, Percent_Identity=32.8502415458937, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25141369, Length=250, Percent_Identity=24, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=226, Percent_Identity=28.7610619469027, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21358017, Length=211, Percent_Identity=31.2796208530806, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357273, Length=305, Percent_Identity=22.9508196721311, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530 [H]

Pfam domain/function: PF00398 RrnaAD [H]

EC number: =2.1.1.182 [H]

Molecular weight: Translated: 32904; Mature: 32904

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSLLSMNINYNSITSIKQTLKERKIAPRKLWGQNYLINESIRQKIIESLDIKENEKIW
CEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCCEECHHHHHHHHHHCCCCCCCCEE
EIGPGLGAMTEILLKKTNLLTAFEIDLKYSEILNEKFGKLKNFKLIKGDFLKKYKNENQN
ECCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCC
IDKIFSNLPYNIASKVISKLIEENFLKEMVFTVQKELADRITAKINSKNYSSFTVLVQSH
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
FKVIKILDIGENNFYPAPKVKSTTLKLIPKKNNIKNFKEFNKLVRTVFSNRRKKLKNTII
CCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFITNKATLRENFLKEYLDKRPENISVEEFIQISNTLNAYH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MILSLLSMNINYNSITSIKQTLKERKIAPRKLWGQNYLINESIRQKIIESLDIKENEKIW
CEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHCCCCCEECHHHHHHHHHHCCCCCCCCEE
EIGPGLGAMTEILLKKTNLLTAFEIDLKYSEILNEKFGKLKNFKLIKGDFLKKYKNENQN
ECCCCHHHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHHHCCCEEHHHHHHHHHCCCCCC
IDKIFSNLPYNIASKVISKLIEENFLKEMVFTVQKELADRITAKINSKNYSSFTVLVQSH
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHC
FKVIKILDIGENNFYPAPKVKSTTLKLIPKKNNIKNFKEFNKLVRTVFSNRRKKLKNTII
CCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFITNKATLRENFLKEYLDKRPENISVEEFIQISNTLNAYH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA