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The map label for this gene is metG

Identifier: 15594932

GI number: 15594932

Start: 604779

End: 606983

Strand: Reverse

Name: metG

Synonym: BB0587

Alternate gene names: 15594932

Gene position: 606983-604779 (Counterclockwise)

Preceding gene: 15594935

Following gene: 15594931

Centisome position: 66.65

GC content: 29.48

Gene sequence:

>2205_bases
ATGAAAAAAATGAATCTAGTTACAGCTGCTCTACCCTATGTTAATAACATACCTCATCTTGGGAATTTAGTTCAAGTGCT
ATCAGCTGATGCTTTTGCAAGATATTCGAAAATGTCAGGAATTGAAACTCTTTACGTCTGCGGAACAGATGAATATGGAA
CAGCTACAGAAACCAAAGCCTTAATTGAAAATACTACCCCTTTAGAACTTTGCAATAAATATTATGAAATACATAAATCA
ATTTACAAATGGTTCAATATTGAATTTGACATCTTTGGTCGCACAACCAACAAGAACCATCAAGACATTGTACAAAATTT
TTTCCTACAATTAGAAAAAAACGGCTATATAAAAGAGAGAGAAACTGAACAGTTTTATTGCAATAAAGATTCAATGTTCT
TGGCTGATAGATATGTAATAGGAGAATGCCCAGAATGCCAAAGCATGGCTAAAGGAGATCAGTGCGACAACTGTTCTAAA
CTTCTAAATCCAACAGACCTAATAAATCCAAAATGCATAATTTGTAAAAACAAGCCTATTTTAAAAAAAACCAATCATCT
TTATTTAGATCTTCCCAAAATAAAAACAAAACTTGAAAAATGGATAAAAAATCCAGATACTAGCAAGAATTGGAATACCA
ATGCCCTTAAAATGACAAAGGCTTTTTTAAGAGATGGCCTTAAAGAAAGGGCAATTACAAGAGACCTGAAATGGGGAATT
CCTGTGCCTAAAAAAGGTTTTGAAAATAAAGTATTTTATGTGTGGTTTGATGCTCCAATAGGATACATTTCAATTACCAA
AAACATTATCAAAAATTGGGAATCTTGGTGGAAAAACAATGATCAAGTAAATCTTGTACAATTTATTGGGAAAGACAATA
TATTGTTTCATACAATTATATTCCCTTGCATAGAAATTGGAAGTGAAGAAAATTGGACAATATTAAATCAACTCTCATCA
AGCGAATACTTAAATTACGAAAATCTTAAATTTTCAAAATCAGAAGGAACAGGAATTTTTGGAAACGATGCTATTACTAC
AGGAATCCCCTCTGATATTTGGCGATTTTATATTTATTATAACAGGCCTGAAAAATCTGATTTTCAATTTATGTGGCAAG
ATCTCATGGAAAGAGTAAATACAGAACTTATTGATAATTTTTCAAACCTTGTAAACAGAGTATTAACATTTCAAAGAAAA
TTCTTTGGAGATGTAATAGAAACAATAGAAATTCAAAATAAGTTTTGGAAACAAATAACACCAAAATATAATAAAATACT
AAATCTTTTTAAAAAGACAGAACTAAAATCTGCTCTCAAAGAAATACTTAAAATTTCTTCCCTTGGAAATAAAATATTTC
AAGATAACGAACCCTGGAAAAGAAAAAACAACTCTCCACAAGAAACAAAAGAACTAATCTCAAACTTAATATACCTAATC
AGAGACTTATCTATTTTAATGATGCCATTCATTCCCGAAACAAGCAAAAAGATACAACAATTCTTTGGCAACAGTTATCA
ATTTTCAACCAAAATTCTTGGAACTAAATCGGGAATTAAAAAAATTGAATTCACAGAAATATTATTCAATAAACTAGAGC
AAAAAAAAATTAATAATTTAAAGCTAAAATATTCAGGAGATAAAAACATGAAAGAAAACGAACAAGCAGAAAACTTGCCT
ATAGCAAAAGAGCAACCGGAAAACTTGTTTAGAGAAAAAGTGCTCTTAAGAGTTGTAAAAATAAATAAAATAGAAAGAAA
TCCCGAGGCTAAAAACTTATTTATATTAAAACTAGATGACGGAACTAACAAGGATAAACAAATAGTAAGCGGCCTTGAAG
GATATTACACAGAAGAAGAACTTTTAGGAAAACATATAATAATAGTAGACAATTTAAAGCCTGCAAAGTTTAGGGGAATA
AAATCTGAAGGAATGCTAATAGCTGCTGAGGACAAAAATAAAAATTTTAAAGTTATAATTGTAGAAGATTCAATTCAAAA
TCCTATTGCTGGAGAGAGAATAATACTTGAAAACGATCAGAATAAAGATCTTGCCTGCCCACCTAAAATTGACATAAATA
AGTTTTTAAAAGCCAATATAGTAGCAGAAAACGGAGAGCTTAAAATAAACGGAATAAATTTAATATTAGAAAATTCTAAG
AACAAAATTTTATCTAAAGATATTCCAAACGGAACAGTTTGCTAA

Upstream 100 bases:

>100_bases
CAAAATTGAATTGCTTTTGAAAGAATTTTTGTAAAAGATATTGAAAAAAAATATCCTATCCATATTACAATATTAATAGC
ATAAAAAAAGGGAAAACTGA

Downstream 100 bases:

>100_bases
GAGCTATTAATGACTATTAAAAAAATAAAAACAAAAGAAATGGAAGAAAATTATCTTCAAAGCGAACTGTGGGCATTAAT
AAAAACAACAAAAAACAGTT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 734; Mature: 734

Protein sequence:

>734_residues
MKKMNLVTAALPYVNNIPHLGNLVQVLSADAFARYSKMSGIETLYVCGTDEYGTATETKALIENTTPLELCNKYYEIHKS
IYKWFNIEFDIFGRTTNKNHQDIVQNFFLQLEKNGYIKERETEQFYCNKDSMFLADRYVIGECPECQSMAKGDQCDNCSK
LLNPTDLINPKCIICKNKPILKKTNHLYLDLPKIKTKLEKWIKNPDTSKNWNTNALKMTKAFLRDGLKERAITRDLKWGI
PVPKKGFENKVFYVWFDAPIGYISITKNIIKNWESWWKNNDQVNLVQFIGKDNILFHTIIFPCIEIGSEENWTILNQLSS
SEYLNYENLKFSKSEGTGIFGNDAITTGIPSDIWRFYIYYNRPEKSDFQFMWQDLMERVNTELIDNFSNLVNRVLTFQRK
FFGDVIETIEIQNKFWKQITPKYNKILNLFKKTELKSALKEILKISSLGNKIFQDNEPWKRKNNSPQETKELISNLIYLI
RDLSILMMPFIPETSKKIQQFFGNSYQFSTKILGTKSGIKKIEFTEILFNKLEQKKINNLKLKYSGDKNMKENEQAENLP
IAKEQPENLFREKVLLRVVKINKIERNPEAKNLFILKLDDGTNKDKQIVSGLEGYYTEEELLGKHIIIVDNLKPAKFRGI
KSEGMLIAAEDKNKNFKVIIVEDSIQNPIAGERIILENDQNKDLACPPKIDINKFLKANIVAENGELKINGINLILENSK
NKILSKDIPNGTVC

Sequences:

>Translated_734_residues
MKKMNLVTAALPYVNNIPHLGNLVQVLSADAFARYSKMSGIETLYVCGTDEYGTATETKALIENTTPLELCNKYYEIHKS
IYKWFNIEFDIFGRTTNKNHQDIVQNFFLQLEKNGYIKERETEQFYCNKDSMFLADRYVIGECPECQSMAKGDQCDNCSK
LLNPTDLINPKCIICKNKPILKKTNHLYLDLPKIKTKLEKWIKNPDTSKNWNTNALKMTKAFLRDGLKERAITRDLKWGI
PVPKKGFENKVFYVWFDAPIGYISITKNIIKNWESWWKNNDQVNLVQFIGKDNILFHTIIFPCIEIGSEENWTILNQLSS
SEYLNYENLKFSKSEGTGIFGNDAITTGIPSDIWRFYIYYNRPEKSDFQFMWQDLMERVNTELIDNFSNLVNRVLTFQRK
FFGDVIETIEIQNKFWKQITPKYNKILNLFKKTELKSALKEILKISSLGNKIFQDNEPWKRKNNSPQETKELISNLIYLI
RDLSILMMPFIPETSKKIQQFFGNSYQFSTKILGTKSGIKKIEFTEILFNKLEQKKINNLKLKYSGDKNMKENEQAENLP
IAKEQPENLFREKVLLRVVKINKIERNPEAKNLFILKLDDGTNKDKQIVSGLEGYYTEEELLGKHIIIVDNLKPAKFRGI
KSEGMLIAAEDKNKNFKVIIVEDSIQNPIAGERIILENDQNKDLACPPKIDINKFLKANIVAENGELKINGINLILENSK
NKILSKDIPNGTVC
>Mature_734_residues
MKKMNLVTAALPYVNNIPHLGNLVQVLSADAFARYSKMSGIETLYVCGTDEYGTATETKALIENTTPLELCNKYYEIHKS
IYKWFNIEFDIFGRTTNKNHQDIVQNFFLQLEKNGYIKERETEQFYCNKDSMFLADRYVIGECPECQSMAKGDQCDNCSK
LLNPTDLINPKCIICKNKPILKKTNHLYLDLPKIKTKLEKWIKNPDTSKNWNTNALKMTKAFLRDGLKERAITRDLKWGI
PVPKKGFENKVFYVWFDAPIGYISITKNIIKNWESWWKNNDQVNLVQFIGKDNILFHTIIFPCIEIGSEENWTILNQLSS
SEYLNYENLKFSKSEGTGIFGNDAITTGIPSDIWRFYIYYNRPEKSDFQFMWQDLMERVNTELIDNFSNLVNRVLTFQRK
FFGDVIETIEIQNKFWKQITPKYNKILNLFKKTELKSALKEILKISSLGNKIFQDNEPWKRKNNSPQETKELISNLIYLI
RDLSILMMPFIPETSKKIQQFFGNSYQFSTKILGTKSGIKKIEFTEILFNKLEQKKINNLKLKYSGDKNMKENEQAENLP
IAKEQPENLFREKVLLRVVKINKIERNPEAKNLFILKLDDGTNKDKQIVSGLEGYYTEEELLGKHIIIVDNLKPAKFRGI
KSEGMLIAAEDKNKNFKVIIVEDSIQNPIAGERIILENDQNKDLACPPKIDINKFLKANIVAENGELKINGINLILENSK
NKILSKDIPNGTVC

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=601, Percent_Identity=45.0915141430948, Blast_Score=552, Evalue=1e-157,
Organism=Homo sapiens, GI24308436, Length=585, Percent_Identity=23.2478632478632, Blast_Score=143, Evalue=7e-34,
Organism=Homo sapiens, GI4507947, Length=99, Percent_Identity=35.3535353535354, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1788432, Length=673, Percent_Identity=28.6775631500743, Blast_Score=296, Evalue=3e-81,
Organism=Escherichia coli, GI1789455, Length=103, Percent_Identity=36.8932038834951, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540794, Length=579, Percent_Identity=45.5958549222798, Blast_Score=528, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI212645096, Length=512, Percent_Identity=23.046875, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71992781, Length=425, Percent_Identity=21.8823529411765, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6321704, Length=559, Percent_Identity=43.8282647584973, Blast_Score=501, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6321610, Length=549, Percent_Identity=25.1366120218579, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24655311, Length=615, Percent_Identity=42.6016260162602, Blast_Score=506, Evalue=1e-143,
Organism=Drosophila melanogaster, GI21358483, Length=516, Percent_Identity=20.9302325581395, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21357223, Length=152, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24651924, Length=79, Percent_Identity=39.2405063291139, Blast_Score=66, Evalue=8e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_BORBU (Q44951)

Other databases:

- EMBL:   AE000783
- EMBL:   L32146
- PIR:   B70173
- RefSeq:   NP_212721.1
- ProteinModelPortal:   Q44951
- EnsemblBacteria:   EBBORT00000008668
- GeneID:   1195434
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0587
- NMPDR:   fig|224326.1.peg.971
- TIGR:   BB_0587
- GeneTree:   EBGT00050000007495
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- PhylomeDB:   Q44951
- ProtClustDB:   PRK00133
- BioCyc:   BBUR224326:BB_0587-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 85215; Mature: 85215

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 333-333

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMNLVTAALPYVNNIPHLGNLVQVLSADAFARYSKMSGIETLYVCGTDEYGTATETKA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
LIENTTPLELCNKYYEIHKSIYKWFNIEFDIFGRTTNKNHQDIVQNFFLQLEKNGYIKER
HHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHEECCCCCCCCC
ETEQFYCNKDSMFLADRYVIGECPECQSMAKGDQCDNCSKLLNPTDLINPKCIICKNKPI
CCCCEEECCCCEEEECCEEECCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCEEEECCCCC
LKKTNHLYLDLPKIKTKLEKWIKNPDTSKNWNTNALKMTKAFLRDGLKERAITRDLKWGI
CCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCC
PVPKKGFENKVFYVWFDAPIGYISITKNIIKNWESWWKNNDQVNLVQFIGKDNILFHTII
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHH
FPCIEIGSEENWTILNQLSSSEYLNYENLKFSKSEGTGIFGNDAITTGIPSDIWRFYIYY
HHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCCEEEEEEE
NRPEKSDFQFMWQDLMERVNTELIDNFSNLVNRVLTFQRKFFGDVIETIEIQNKFWKQIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKYNKILNLFKKTELKSALKEILKISSLGNKIFQDNEPWKRKNNSPQETKELISNLIYLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
RDLSILMMPFIPETSKKIQQFFGNSYQFSTKILGTKSGIKKIEFTEILFNKLEQKKINNL
HHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCE
KLKYSGDKNMKENEQAENLPIAKEQPENLFREKVLLRVVKINKIERNPEAKNLFILKLDD
EEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
GTNKDKQIVSGLEGYYTEEELLGKHIIIVDNLKPAKFRGIKSEGMLIAAEDKNKNFKVII
CCCHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCHHHCCCCCCCEEEEEECCCCCEEEEE
VEDSIQNPIAGERIILENDQNKDLACPPKIDINKFLKANIVAENGELKINGINLILENSK
EECCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHEEEEEECCCEEEEEEEEEEEECCC
NKILSKDIPNGTVC
CCCCCCCCCCCCCC
>Mature Secondary Structure
MKKMNLVTAALPYVNNIPHLGNLVQVLSADAFARYSKMSGIETLYVCGTDEYGTATETKA
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHH
LIENTTPLELCNKYYEIHKSIYKWFNIEFDIFGRTTNKNHQDIVQNFFLQLEKNGYIKER
HHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHEECCCCCCCCC
ETEQFYCNKDSMFLADRYVIGECPECQSMAKGDQCDNCSKLLNPTDLINPKCIICKNKPI
CCCCEEECCCCEEEECCEEECCCCCHHHHHCCCCCCCHHHHCCCCCCCCCCEEEECCCCC
LKKTNHLYLDLPKIKTKLEKWIKNPDTSKNWNTNALKMTKAFLRDGLKERAITRDLKWGI
CCCCCEEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCC
PVPKKGFENKVFYVWFDAPIGYISITKNIIKNWESWWKNNDQVNLVQFIGKDNILFHTII
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEHHHHH
FPCIEIGSEENWTILNQLSSSEYLNYENLKFSKSEGTGIFGNDAITTGIPSDIWRFYIYY
HHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCCEEEEEEE
NRPEKSDFQFMWQDLMERVNTELIDNFSNLVNRVLTFQRKFFGDVIETIEIQNKFWKQIT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKYNKILNLFKKTELKSALKEILKISSLGNKIFQDNEPWKRKNNSPQETKELISNLIYLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
RDLSILMMPFIPETSKKIQQFFGNSYQFSTKILGTKSGIKKIEFTEILFNKLEQKKINNL
HHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCE
KLKYSGDKNMKENEQAENLPIAKEQPENLFREKVLLRVVKINKIERNPEAKNLFILKLDD
EEEECCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
GTNKDKQIVSGLEGYYTEEELLGKHIIIVDNLKPAKFRGIKSEGMLIAAEDKNKNFKVII
CCCHHHHHHHHHHCCCCCHHHCCCEEEEEECCCCHHHCCCCCCCEEEEEECCCCCEEEEE
VEDSIQNPIAGERIILENDQNKDLACPPKIDINKFLKANIVAENGELKINGINLILENSK
EECCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHEEEEEECCCEEEEEEEEEEEECCC
NKILSKDIPNGTVC
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 7812434