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The map label for this gene is yjfH [C]

Identifier: 15594861

GI number: 15594861

Start: 527361

End: 528047

Strand: Reverse

Name: yjfH [C]

Synonym: BB0516

Alternate gene names: 15594861

Gene position: 528047-527361 (Counterclockwise)

Preceding gene: 15594862

Following gene: 15594852

Centisome position: 57.98

GC content: 26.78

Gene sequence:

>687_bases
TTGAACTTTATATATCAAAAAAAAAGTCCAAAAACTAAAGAAATTGAACAGCTAGCTAAAACACAAAATATAAAAATAAT
AAGAATAAATACAAATGAACTTGACAAAATTCTTAAAAACAAAGACCACAGAGGATTTGCATTAAAATTAAAACTTGAAA
AAAATAAAAATGTAAAAACACAAACCAAAGATTTTGAAAACTTACTAGAAACATTTAAAAAAAAAGAAAATGCTTTCATT
TTACTTCTAGATGAAATAGAAGATCCCCAAAACTTTGGAGCAATCCTTAGAACAGCAGAACAGTTTAGTATAGATCTTGT
AATTACTACTCAAAAACGCAGCGCAAAAGACAATTCAACAGTTTTGCGCACTAGCTCTGGCGCAAGTCAATATGTAAAAA
AAATGACAGTGACAAACATAAACAACACAATAAATCTTTTAAAAAACTATGGCTTTTGGATATATACTGGTGATATTAAA
GGACAAGATATAAACAAAATCAAAATAAACGATAAAAAAATTGCACTTATTTTAGGAAATGAAGGAAAAGGTGTACATAA
GCTAATAAAAGAAAATTCTGATTTTTTAATAAGGATACCAACCAGCGGAAAAATAGACTCACTTAATGTCTCTGTATCTA
CAGGCATTTTAATTTTTGAAATCAAAAGACAATTAAATCTACTCTAA

Upstream 100 bases:

>100_bases
AAGAATTAAAAGATGGTGATGAAATAGATTTACTCAAAGCATAATATGTATATAACTCATGCCAATTCAATAATTGAAAG
CATAAAAAATAACAAAGGAT

Downstream 100 bases:

>100_bases
AGCATTAAATTATATAAAAACTTCATTTAAAAAAACAAAAACATCTTAATAAAAACTATTTTAAAAAATTTCCTAACTCA
ACAGATGCAATAAACCCCTC

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MNFIYQKKSPKTKEIEQLAKTQNIKIIRINTNELDKILKNKDHRGFALKLKLEKNKNVKTQTKDFENLLETFKKKENAFI
LLLDEIEDPQNFGAILRTAEQFSIDLVITTQKRSAKDNSTVLRTSSGASQYVKKMTVTNINNTINLLKNYGFWIYTGDIK
GQDINKIKINDKKIALILGNEGKGVHKLIKENSDFLIRIPTSGKIDSLNVSVSTGILIFEIKRQLNLL

Sequences:

>Translated_228_residues
MNFIYQKKSPKTKEIEQLAKTQNIKIIRINTNELDKILKNKDHRGFALKLKLEKNKNVKTQTKDFENLLETFKKKENAFI
LLLDEIEDPQNFGAILRTAEQFSIDLVITTQKRSAKDNSTVLRTSSGASQYVKKMTVTNINNTINLLKNYGFWIYTGDIK
GQDINKIKINDKKIALILGNEGKGVHKLIKENSDFLIRIPTSGKIDSLNVSVSTGILIFEIKRQLNLL
>Mature_228_residues
MNFIYQKKSPKTKEIEQLAKTQNIKIIRINTNELDKILKNKDHRGFALKLKLEKNKNVKTQTKDFENLLETFKKKENAFI
LLLDEIEDPQNFGAILRTAEQFSIDLVITTQKRSAKDNSTVLRTSSGASQYVKKMTVTNINNTINLLKNYGFWIYTGDIK
GQDINKIKINDKKIALILGNEGKGVHKLIKENSDFLIRIPTSGKIDSLNVSVSTGILIFEIKRQLNLL

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family

Homologues:

Organism=Homo sapiens, GI40068479, Length=232, Percent_Identity=24.1379310344828, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1790623, Length=206, Percent_Identity=33.495145631068, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1788935, Length=223, Percent_Identity=22.8699551569507, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=163, Percent_Identity=30.6748466257669, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y516_BORBU (O51468)

Other databases:

- EMBL:   AE000783
- PIR:   C70164
- RefSeq:   NP_212650.1
- ProteinModelPortal:   O51468
- SMR:   O51468
- EnsemblBacteria:   EBBORT00000008700
- GeneID:   1195362
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0516
- NMPDR:   fig|224326.1.peg.900
- TIGR:   BB_0516
- GeneTree:   EBGT00050000006967
- HOGENOM:   HBG714057
- OMA:   LIRIPTS
- ProtClustDB:   CLSK507630
- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123
- TIGRFAMs:   TIGR00186

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26082; Mature: 26082

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: BINDING 179-179 BINDING 199-199 BINDING 208-208

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIYQKKSPKTKEIEQLAKTQNIKIIRINTNELDKILKNKDHRGFALKLKLEKNKNVKT
CCCCCCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCEEEEEEEECCCCCCC
QTKDFENLLETFKKKENAFILLLDEIEDPQNFGAILRTAEQFSIDLVITTQKRSAKDNST
HHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCCCCE
VLRTSSGASQYVKKMTVTNINNTINLLKNYGFWIYTGDIKGQDINKIKINDKKIALILGN
EEEECCCHHHHHHHHHEECCCHHHHHHHHCCEEEEECCCCCCCCEEEEECCCEEEEEECC
EGKGVHKLIKENSDFLIRIPTSGKIDSLNVSVSTGILIFEIKRQLNLL
CCCHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCEEEEEEEHHHCCC
>Mature Secondary Structure
MNFIYQKKSPKTKEIEQLAKTQNIKIIRINTNELDKILKNKDHRGFALKLKLEKNKNVKT
CCCCCCCCCCCHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCEEEEEEEECCCCCCC
QTKDFENLLETFKKKENAFILLLDEIEDPQNFGAILRTAEQFSIDLVITTQKRSAKDNST
HHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHEEEEEEEEECCCCCCCCCE
VLRTSSGASQYVKKMTVTNINNTINLLKNYGFWIYTGDIKGQDINKIKINDKKIALILGN
EEEECCCHHHHHHHHHEECCCHHHHHHHHCCEEEEECCCCCCCCEEEEECCCEEEEEECC
EGKGVHKLIKENSDFLIRIPTSGKIDSLNVSVSTGILIFEIKRQLNLL
CCCHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCEEEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9403685