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The map label for this gene is mscS [H]

Identifier: 15594798

GI number: 15594798

Start: 472566

End: 473408

Strand: Direct

Name: mscS [H]

Synonym: BB0453

Alternate gene names: 15594798

Gene position: 472566-473408 (Clockwise)

Preceding gene: 15594790

Following gene: 15594800

Centisome position: 51.89

GC content: 27.16

Gene sequence:

>843_bases
TTGTTTAAGGAGTTTTTTATATTTCAAGATTATTTTAATTATATTATTGAGGGCGTGGTAGGTTATGGTCTAAAAGTTTC
GATTGCTATAGTGCTATGGTATTTTTTAAAGTTAATAGTTAGTAAAATGGGAAAAATTTTATTTAAAACTTTAGAAAAGT
CCAGATTAGAGGAAAAGTTAGAAGTTACAGTTTTTAACTTTTTAAAATCTTTTTTTAAAATATTAACAGACTTTGTTATT
GTTTTAATAATATTGCCATATCTTGGGGTGCCTACAACATCTATTATTGCTGTATTTGGATCATTAGGGCTTGCCATTGG
GCTTGCTGCTCAGAATATTCTATCTAATTTTGTTAGTGGATTTATTGTTTTGAATTCTAAGTTTTTTAAGTGTGGAGATC
ATATTCAATGTGGAGATGTTGAAGGTTTGGTTGCAGATGTCCAAATTTTTTTTACTACACTTGAAACATTTAACAAAGAA
ATTATTAAAATTCCAAACAGTAAGCTTACATCCAATTTTGTTGTTAATTTTTCGTCATGTACTAGAAGAAGAGTTGTGTT
TTCTTTTCAAGTTCCCTATGATACGAATATTGGTTTATTAAAGGATAAAATAGAGGATTTAATGATTTTCAATAATAAGA
AATTTAATGTTGAGCTTTGTGCTCCTACTCTTATTGTTAAAAAATACACTCCTTATTATATAGTTTTGCAAGTTCGATTT
TTTGTTAATACAGAGGTTTTTTGGGATTTTCAATATTTTGTTGGTGAGTCTATTCAAAATGTTTTATTGGATATGAAAAT
TAAGGTTCCAATTTGTTTTGTGCCTTTTGATAAGCTGTATTAA

Upstream 100 bases:

>100_bases
ATTGAGTCATTGTTTTAATTAGGGTTTTTATTGGAAGGCTTGTTTGTTTTTTGTGCTATAATTCAATTAATTTATGTAAA
GCCCGGAAGGAAAGTTTAAT

Downstream 100 bases:

>100_bases
TGATTTTTTCTTGCAATAATTTCTGAGTAATAGTTTTTAATTTTTTTTGTGAAAAAATAGCTTGAAAATTTAGTGGAGTG
TTTTTTTGCATTTTCTTTAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MFKEFFIFQDYFNYIIEGVVGYGLKVSIAIVLWYFLKLIVSKMGKILFKTLEKSRLEEKLEVTVFNFLKSFFKILTDFVI
VLIILPYLGVPTTSIIAVFGSLGLAIGLAAQNILSNFVSGFIVLNSKFFKCGDHIQCGDVEGLVADVQIFFTTLETFNKE
IIKIPNSKLTSNFVVNFSSCTRRRVVFSFQVPYDTNIGLLKDKIEDLMIFNNKKFNVELCAPTLIVKKYTPYYIVLQVRF
FVNTEVFWDFQYFVGESIQNVLLDMKIKVPICFVPFDKLY

Sequences:

>Translated_280_residues
MFKEFFIFQDYFNYIIEGVVGYGLKVSIAIVLWYFLKLIVSKMGKILFKTLEKSRLEEKLEVTVFNFLKSFFKILTDFVI
VLIILPYLGVPTTSIIAVFGSLGLAIGLAAQNILSNFVSGFIVLNSKFFKCGDHIQCGDVEGLVADVQIFFTTLETFNKE
IIKIPNSKLTSNFVVNFSSCTRRRVVFSFQVPYDTNIGLLKDKIEDLMIFNNKKFNVELCAPTLIVKKYTPYYIVLQVRF
FVNTEVFWDFQYFVGESIQNVLLDMKIKVPICFVPFDKLY
>Mature_280_residues
MFKEFFIFQDYFNYIIEGVVGYGLKVSIAIVLWYFLKLIVSKMGKILFKTLEKSRLEEKLEVTVFNFLKSFFKILTDFVI
VLIILPYLGVPTTSIIAVFGSLGLAIGLAAQNILSNFVSGFIVLNSKFFKCGDHIQCGDVEGLVADVQIFFTTLETFNKE
IIKIPNSKLTSNFVVNFSSCTRRRVVFSFQVPYDTNIGLLKDKIEDLMIFNNKKFNVELCAPTLIVKKYTPYYIVLQVRF
FVNTEVFWDFQYFVGESIQNVLLDMKIKVPICFVPFDKLY

Specific function: Mechanosensitive channel that participates in the regulation of osmotic pressure changes within the cell. Forms an ion channel of 1.0 nanosiemens conductance with a slight preference for anions. The channel is sensitive to voltage [H]

COG id: COG0668

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1789291, Length=221, Percent_Identity=30.7692307692308, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787591, Length=208, Percent_Identity=25.9615384615385, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014
- InterPro:   IPR008910 [H]

Pfam domain/function: PF00924 MS_channel; PF05552 TM_helix [H]

EC number: NA

Molecular weight: Translated: 32303; Mature: 32303

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEFFIFQDYFNYIIEGVVGYGLKVSIAIVLWYFLKLIVSKMGKILFKTLEKSRLEEKL
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVTVFNFLKSFFKILTDFVIVLIILPYLGVPTTSIIAVFGSLGLAIGLAAQNILSNFVSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIVLNSKFFKCGDHIQCGDVEGLVADVQIFFTTLETFNKEIIKIPNSKLTSNFVVNFSSC
EEEECCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCEEEEECCC
TRRRVVFSFQVPYDTNIGLLKDKIEDLMIFNNKKFNVELCAPTLIVKKYTPYYIVLQVRF
CCEEEEEEEECCCCCCCHHHHHHHHHHEEECCCEEEEEEECCEEEEECCCCEEEEEEEEE
FVNTEVFWDFQYFVGESIQNVLLDMKIKVPICFVPFDKLY
EECCEEEEEHHHHHHHHHHHHHHHEEEECEEEEECHHHCC
>Mature Secondary Structure
MFKEFFIFQDYFNYIIEGVVGYGLKVSIAIVLWYFLKLIVSKMGKILFKTLEKSRLEEKL
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EVTVFNFLKSFFKILTDFVIVLIILPYLGVPTTSIIAVFGSLGLAIGLAAQNILSNFVSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIVLNSKFFKCGDHIQCGDVEGLVADVQIFFTTLETFNKEIIKIPNSKLTSNFVVNFSSC
EEEECCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHEEECCCCCCCCCEEEEECCC
TRRRVVFSFQVPYDTNIGLLKDKIEDLMIFNNKKFNVELCAPTLIVKKYTPYYIVLQVRF
CCEEEEEEEECCCCCCCHHHHHHHHHHEEECCCEEEEEEECCEEEEECCCCEEEEEEEEE
FVNTEVFWDFQYFVGESIQNVLLDMKIKVPICFVPFDKLY
EECCEEEEEHHHHHHHHHHHHHHHEEEECEEEEECHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]