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The map label for this gene is dnaN [H]

Identifier: 15594783

GI number: 15594783

Start: 458277

End: 459434

Strand: Direct

Name: dnaN [H]

Synonym: BB0438

Alternate gene names: 15594783

Gene position: 458277-459434 (Clockwise)

Preceding gene: 15594782

Following gene: 15594784

Centisome position: 50.32

GC content: 25.65

Gene sequence:

>1158_bases
ATGCTACACAACACATTTTTTATTTGCGAAACAAATCAAATTATGAATGAGATAGAAAAAGCAAAGGGAATAATATTAAA
CAGAAATATGAATGATATTTGGAGTGCATTGTTAATAGAAGTAAAAAAATCTAATCTCATAATCAAATCAACAGACAGAA
ATATATTTTTTGAAAGCACAATTTCAATTGTTTCAGAAACAGATTTTAAGGTATTAATTAATGCTTCAAATTTTTATGAT
GCAGTAAAAGCATTCAGTTTTTATAAGAAAATCAAAATAGTTTTTAATGAAAATAATAGTAAATTAGAAATTATGGGAGA
GTTAAATGATGAGAAAGAGGAATATGAAGATCATTTAAAAGAGCCCACTTTTTCATACGAAGAAATAGAAAATTACAATT
ATGATATGGTTAATGAAGATTATACTTTTGGAATTGAAATAAAACAAAAATCTTTTAAAAAAGTAATAAATAGAATAGCT
TTTTCAGCACATCTTGATGAATCTAAGAATGTATTAAATGGTGTGTATTTTTCAAAAGATGAAGATTCTAAATTACTACT
TGTTTCTACTAATGGTCACAGAATGTCTATTTGTAAAACAGAAGTCATAGTTGAAGAAGATGTGAATTTTATAGTTCCTG
TAAAAATATTTAATTTCTTAAAACATCTTATGTCGGGAGAAGGTATGGTTAAAATAAAGTTTTCGGATAAAAAATTTTAT
GTTGAATTTGATAATTATAAAATAGCTTGTAGCTTAATTAATGGTAATTATCCGGATTATAAAAGCATTATACCTAAAGA
ACAGAAAAATAAATCTTTAGTTTCGCTAGGCATTTTAAAAGATAGACTTGCAAGAGTCAATTTATATGTTGATAAATCAA
GAAAGTTGGTTTTAACTTTCTCTGAGTTACAATTAAAACTTCTTGGAGAAGATTTGATTACTGGAAGAAAAGGTGAATTT
TTCATTAAAGATCCAAATTATCTGTATGACGGAGCAGATGAGGTTATGGCTATTAATATTTCATATTTTGTTGAAGCTAT
TAGTGTTTTTGAAACTTCAAAGATAGAAATACAATTTAATTCGGGCAATGTATTAAAATTGAGCGAACCTGAAAATTTTA
ATTTTACGCACTTGATAATGCCGATGTCTTTGGGTTAA

Upstream 100 bases:

>100_bases
AGCTTACTACTATTACTACTATATATCTATAAAAAATAAAAATACAAAATCCACAAAATCTTATTAAGATAAAATGTAAT
AGAATATATAAGGAGGCATC

Downstream 100 bases:

>100_bases
AGTGAGTTATTAATGCTATTTTGAATATTAAAATAAGCTTTTCTAGGTTTATTCAATATTGTTTTTTGCATTGTGGTATA
AAAAATTGATATGAATGATT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MLHNTFFICETNQIMNEIEKAKGIILNRNMNDIWSALLIEVKKSNLIIKSTDRNIFFESTISIVSETDFKVLINASNFYD
AVKAFSFYKKIKIVFNENNSKLEIMGELNDEKEEYEDHLKEPTFSYEEIENYNYDMVNEDYTFGIEIKQKSFKKVINRIA
FSAHLDESKNVLNGVYFSKDEDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKFSDKKFY
VEFDNYKIACSLINGNYPDYKSIIPKEQKNKSLVSLGILKDRLARVNLYVDKSRKLVLTFSELQLKLLGEDLITGRKGEF
FIKDPNYLYDGADEVMAINISYFVEAISVFETSKIEIQFNSGNVLKLSEPENFNFTHLIMPMSLG

Sequences:

>Translated_385_residues
MLHNTFFICETNQIMNEIEKAKGIILNRNMNDIWSALLIEVKKSNLIIKSTDRNIFFESTISIVSETDFKVLINASNFYD
AVKAFSFYKKIKIVFNENNSKLEIMGELNDEKEEYEDHLKEPTFSYEEIENYNYDMVNEDYTFGIEIKQKSFKKVINRIA
FSAHLDESKNVLNGVYFSKDEDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKFSDKKFY
VEFDNYKIACSLINGNYPDYKSIIPKEQKNKSLVSLGILKDRLARVNLYVDKSRKLVLTFSELQLKLLGEDLITGRKGEF
FIKDPNYLYDGADEVMAINISYFVEAISVFETSKIEIQFNSGNVLKLSEPENFNFTHLIMPMSLG
>Mature_385_residues
MLHNTFFICETNQIMNEIEKAKGIILNRNMNDIWSALLIEVKKSNLIIKSTDRNIFFESTISIVSETDFKVLINASNFYD
AVKAFSFYKKIKIVFNENNSKLEIMGELNDEKEEYEDHLKEPTFSYEEIENYNYDMVNEDYTFGIEIKQKSFKKVINRIA
FSAHLDESKNVLNGVYFSKDEDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKFSDKKFY
VEFDNYKIACSLINGNYPDYKSIIPKEQKNKSLVSLGILKDRLARVNLYVDKSRKLVLTFSELQLKLLGEDLITGRKGEF
FIKDPNYLYDGADEVMAINISYFVEAISVFETSKIEIQFNSGNVLKLSEPENFNFTHLIMPMSLG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=385, Percent_Identity=20, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 44615; Mature: 44615

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHNTFFICETNQIMNEIEKAKGIILNRNMNDIWSALLIEVKKSNLIIKSTDRNIFFEST
CCCCEEEEEECHHHHHHHHHHCCEEEECCHHHHHHHHHEEEECCCEEEEECCCCEEEEEE
ISIVSETDFKVLINASNFYDAVKAFSFYKKIKIVFNENNSKLEIMGELNDEKEEYEDHLK
EEEEECCCEEEEEECCHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHC
EPTFSYEEIENYNYDMVNEDYTFGIEIKQKSFKKVINRIAFSAHLDESKNVLNGVYFSKD
CCCCCHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHCCEEECCC
EDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKFSDKKFY
CCCEEEEEECCCCEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHCCCCEEEEEECCCEEE
VEFDNYKIACSLINGNYPDYKSIIPKEQKNKSLVSLGILKDRLARVNLYVDKSRKLVLTF
EEECCEEEEEEEECCCCCCHHHCCCCCCCCCCEEEEHHHHHHHEEEEEEEECCCEEEEEH
SELQLKLLGEDLITGRKGEFFIKDPNYLYDGADEVMAINISYFVEAISVFETSKIEIQFN
HHHEEEECCHHHHCCCCCCEEEECCCEEECCCCCEEEEEHHHHHHHHHHEECEEEEEEEC
SGNVLKLSEPENFNFTHLIMPMSLG
CCCEEEECCCCCCCEEEEEEEECCC
>Mature Secondary Structure
MLHNTFFICETNQIMNEIEKAKGIILNRNMNDIWSALLIEVKKSNLIIKSTDRNIFFEST
CCCCEEEEEECHHHHHHHHHHCCEEEECCHHHHHHHHHEEEECCCEEEEECCCCEEEEEE
ISIVSETDFKVLINASNFYDAVKAFSFYKKIKIVFNENNSKLEIMGELNDEKEEYEDHLK
EEEEECCCEEEEEECCHHHHHHHHHHHEEEEEEEEECCCCEEEEEECCCCCHHHHHHHHC
EPTFSYEEIENYNYDMVNEDYTFGIEIKQKSFKKVINRIAFSAHLDESKNVLNGVYFSKD
CCCCCHHHHCCCCCCCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHCCEEECCC
EDSKLLLVSTNGHRMSICKTEVIVEEDVNFIVPVKIFNFLKHLMSGEGMVKIKFSDKKFY
CCCEEEEEECCCCEEEEEEEEEEEECCCCEEEHHHHHHHHHHHHCCCCEEEEEECCCEEE
VEFDNYKIACSLINGNYPDYKSIIPKEQKNKSLVSLGILKDRLARVNLYVDKSRKLVLTF
EEECCEEEEEEEECCCCCCHHHCCCCCCCCCCEEEEHHHHHHHEEEEEEEECCCEEEEEH
SELQLKLLGEDLITGRKGEFFIKDPNYLYDGADEVMAINISYFVEAISVFETSKIEIQFN
HHHEEEECCHHHHCCCCCCEEEECCCEEECCCCCEEEEEHHHHHHHHHHEECEEEEEEEC
SGNVLKLSEPENFNFTHLIMPMSLG
CCCEEEECCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8341609; 9403685; 8359672 [H]