Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 15594717

GI number: 15594717

Start: 381910

End: 383382

Strand: Reverse

Name: gltX [H]

Synonym: BB0372

Alternate gene names: 15594717

Gene position: 383382-381910 (Counterclockwise)

Preceding gene: 15594718

Following gene: 15594716

Centisome position: 42.1

GC content: 31.5

Gene sequence:

>1473_bases
TTGAGTACAAGAGTTCGTTATGCGCCTTCTCCAACGGGTTTACAACATATTGGCGGGATTAGAACAGCTTTGTTTAATTA
TTTTTTTGCAAAGTCTTGCGGAGGTAAATTTTTGCTTAGAATTGAGGATACAGATCAGAGCAGGTATTCTCCAGAAGCTG
AAAATGATCTTTATTCAAGTCTTAAATGGCTTGGCATTTCTTTTGATGAAGGCCCTGTTGTAGGGGGTGATTATGCGCCT
TATGTTCAGTCTCAAAGAAGTGCAATATATAAGCAATATGCTAAATATTTGATTGAATCTGGGCATGCTTATTATTGTTA
TTGCAGTCCCGAAAGGCTTGAAAGAATTAAGAAAATTCAAAATATTAATAAGATGCCACCTGGATATGATAGGCATTGCA
GGAATTTAAGTAATGAAGAGGTTGAGAATGCACTAATTAAAAAAATCAAGCCTGTTGTTAGATTTAAAATTCCTTTAGAA
GGAGATACCAGCTTTGATGATATTTTACTTGGAAGGATTACATGGGCGAATAAAGACATTAGTCCTGATCCTGTAATTCT
TAAGTCAGATGGATTGCCGACTTATCATCTTGCCAATGTTGTTGATGATTATTTAATGAAAATTACCCATGTATTAAGGG
CTCAAGAATGGGTTTCTTCAGGTCCATTGCACGTACTTCTTTATAAGGCTTTTAAATGGAAACCCCCTATTTATTGTCAC
CTTCCAATGGTTATGGGAAATGATGGTCAAAAATTAAGCAAAAGACATGGCTCAACAGCTTTAAGACAGTTTATTGAAGA
TGGGTATCTTCCAGAGGCTATTATTAATTATGTTACTTTGCTTGGCTGGTCTTACGACGATAAGAGAGAATTTTTTTCAA
AAAATGATCTTGAGCAATTTTTTTCAATTGAGAAGATCAATAAATCTCCTGCTATTTTTGATTATCATAAATTGGATTTT
TTCAATAGCTACTATATTAGAGAAAAAAAAGATGAAGATTTATTTAATCTTTTACTCCCTTTTTTCCAAAAAAAAGGGTA
TGTTTCTAAGCCTAGTACTTTGGAAGAAAATCAAAAATTAAAGTTATTAATTCCTCTTATAAAGAGTAGAATTAAAAAAT
TAAGTGATGCTTTAAATATGACTAAATTTTTTTATGAGGACATTAAATCTTGGAATTTAGATGAGTTTTTAAGTAGAAAA
AAAACAGCTAAAGAAGTTTGTTCTATTTTAGAATTAATAAAGCCTATTTTAGAAGGGTTTGAAAAAAGATCATCAGAAGA
AAATGATAAAATTTTTTATGATTTTGCTGAGAGTAATGGTTTTAAATTGGGAGAAATTCTTCTTCCTATTAGAATTGCAG
CGCTTGGTAGCAAAGTCTCTCCGCCGCTTTTTGATTCTTTAAAATTGATAGGCAAGTCTAAAGTTTTTGAAAGAATAAAA
TTAGCACAGGAATTTTTAAGAATAAATGAATAG

Upstream 100 bases:

>100_bases
TAAGCATACAAAGCTACTTGGTTCGAATTTATTTTTTGTTGTTTTTTATTATATTTATGATTTTTCTAAAAAAGATGTTT
TTTATTAAAGGAGTTGTGTT

Downstream 100 bases:

>100_bases
CTATTAAGGATATTTTTATGGTTAGAATGGAAGATATTATTTCTCTTGCAAAAAGAAAAGGATTTGTATTTCAGTCTTCA
GAGGTTTACGGGGGCCTTTC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSTRVRYAPSPTGLQHIGGIRTALFNYFFAKSCGGKFLLRIEDTDQSRYSPEAENDLYSSLKWLGISFDEGPVVGGDYAP
YVQSQRSAIYKQYAKYLIESGHAYYCYCSPERLERIKKIQNINKMPPGYDRHCRNLSNEEVENALIKKIKPVVRFKIPLE
GDTSFDDILLGRITWANKDISPDPVILKSDGLPTYHLANVVDDYLMKITHVLRAQEWVSSGPLHVLLYKAFKWKPPIYCH
LPMVMGNDGQKLSKRHGSTALRQFIEDGYLPEAIINYVTLLGWSYDDKREFFSKNDLEQFFSIEKINKSPAIFDYHKLDF
FNSYYIREKKDEDLFNLLLPFFQKKGYVSKPSTLEENQKLKLLIPLIKSRIKKLSDALNMTKFFYEDIKSWNLDEFLSRK
KTAKEVCSILELIKPILEGFEKRSSEENDKIFYDFAESNGFKLGEILLPIRIAALGSKVSPPLFDSLKLIGKSKVFERIK
LAQEFLRINE

Sequences:

>Translated_490_residues
MSTRVRYAPSPTGLQHIGGIRTALFNYFFAKSCGGKFLLRIEDTDQSRYSPEAENDLYSSLKWLGISFDEGPVVGGDYAP
YVQSQRSAIYKQYAKYLIESGHAYYCYCSPERLERIKKIQNINKMPPGYDRHCRNLSNEEVENALIKKIKPVVRFKIPLE
GDTSFDDILLGRITWANKDISPDPVILKSDGLPTYHLANVVDDYLMKITHVLRAQEWVSSGPLHVLLYKAFKWKPPIYCH
LPMVMGNDGQKLSKRHGSTALRQFIEDGYLPEAIINYVTLLGWSYDDKREFFSKNDLEQFFSIEKINKSPAIFDYHKLDF
FNSYYIREKKDEDLFNLLLPFFQKKGYVSKPSTLEENQKLKLLIPLIKSRIKKLSDALNMTKFFYEDIKSWNLDEFLSRK
KTAKEVCSILELIKPILEGFEKRSSEENDKIFYDFAESNGFKLGEILLPIRIAALGSKVSPPLFDSLKLIGKSKVFERIK
LAQEFLRINE
>Mature_489_residues
STRVRYAPSPTGLQHIGGIRTALFNYFFAKSCGGKFLLRIEDTDQSRYSPEAENDLYSSLKWLGISFDEGPVVGGDYAPY
VQSQRSAIYKQYAKYLIESGHAYYCYCSPERLERIKKIQNINKMPPGYDRHCRNLSNEEVENALIKKIKPVVRFKIPLEG
DTSFDDILLGRITWANKDISPDPVILKSDGLPTYHLANVVDDYLMKITHVLRAQEWVSSGPLHVLLYKAFKWKPPIYCHL
PMVMGNDGQKLSKRHGSTALRQFIEDGYLPEAIINYVTLLGWSYDDKREFFSKNDLEQFFSIEKINKSPAIFDYHKLDFF
NSYYIREKKDEDLFNLLLPFFQKKGYVSKPSTLEENQKLKLLIPLIKSRIKKLSDALNMTKFFYEDIKSWNLDEFLSRKK
TAKEVCSILELIKPILEGFEKRSSEENDKIFYDFAESNGFKLGEILLPIRIAALGSKVSPPLFDSLKLIGKSKVFERIKL
AQEFLRINE

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=503, Percent_Identity=36.779324055666, Blast_Score=309, Evalue=5e-84,
Organism=Escherichia coli, GI1788743, Length=487, Percent_Identity=34.9075975359343, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI226510925, Length=256, Percent_Identity=35.15625, Blast_Score=138, Evalue=8e-34,
Organism=Caenorhabditis elegans, GI71993394, Length=489, Percent_Identity=35.9918200408998, Blast_Score=288, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6324540, Length=505, Percent_Identity=34.6534653465347, Blast_Score=287, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6324742, Length=229, Percent_Identity=26.2008733624454, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321192, Length=246, Percent_Identity=26.4227642276423, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=38.0761523046092, Blast_Score=330, Evalue=1e-90,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56752; Mature: 56621

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTRVRYAPSPTGLQHIGGIRTALFNYFFAKSCGGKFLLRIEDTDQSRYSPEAENDLYSS
CCCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
LKWLGISFDEGPVVGGDYAPYVQSQRSAIYKQYAKYLIESGHAYYCYCSPERLERIKKIQ
HHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHH
NINKMPPGYDRHCRNLSNEEVENALIKKIKPVVRFKIPLEGDTSFDDILLGRITWANKDI
HCCCCCCCHHHHHCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHEEEECCCCC
SPDPVILKSDGLPTYHLANVVDDYLMKITHVLRAQEWVSSGPLHVLLYKAFKWKPPIYCH
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCEEEE
LPMVMGNDGQKLSKRHGSTALRQFIEDGYLPEAIINYVTLLGWSYDDKREFFSKNDLEQF
ECEEECCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHH
FSIEKINKSPAIFDYHKLDFFNSYYIREKKDEDLFNLLLPFFQKKGYVSKPSTLEENQKL
HHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
KLLIPLIKSRIKKLSDALNMTKFFYEDIKSWNLDEFLSRKKTAKEVCSILELIKPILEGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKRSSEENDKIFYDFAESNGFKLGEILLPIRIAALGSKVSPPLFDSLKLIGKSKVFERIK
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LAQEFLRINE
HHHHHHHCCC
>Mature Secondary Structure 
STRVRYAPSPTGLQHIGGIRTALFNYFFAKSCGGKFLLRIEDTDQSRYSPEAENDLYSS
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHH
LKWLGISFDEGPVVGGDYAPYVQSQRSAIYKQYAKYLIESGHAYYCYCSPERLERIKKIQ
HHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHH
NINKMPPGYDRHCRNLSNEEVENALIKKIKPVVRFKIPLEGDTSFDDILLGRITWANKDI
HCCCCCCCHHHHHCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCHHHHHHHEEEECCCCC
SPDPVILKSDGLPTYHLANVVDDYLMKITHVLRAQEWVSSGPLHVLLYKAFKWKPPIYCH
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCEEEE
LPMVMGNDGQKLSKRHGSTALRQFIEDGYLPEAIINYVTLLGWSYDDKREFFSKNDLEQF
ECEEECCCCHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHH
FSIEKINKSPAIFDYHKLDFFNSYYIREKKDEDLFNLLLPFFQKKGYVSKPSTLEENQKL
HHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
KLLIPLIKSRIKKLSDALNMTKFFYEDIKSWNLDEFLSRKKTAKEVCSILELIKPILEGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKRSSEENDKIFYDFAESNGFKLGEILLPIRIAALGSKVSPPLFDSLKLIGKSKVFERIK
HHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LAQEFLRINE
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA