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The map label for this gene is pcrA [H]

Identifier: 15594689

GI number: 15594689

Start: 351341

End: 353440

Strand: Reverse

Name: pcrA [H]

Synonym: BB0344

Alternate gene names: 15594689

Gene position: 353440-351341 (Counterclockwise)

Preceding gene: 15594690

Following gene: 15594688

Centisome position: 38.81

GC content: 26.25

Gene sequence:

>2100_bases
GTGATGGATAAAATAAAAAAATTTTTTTCTAGCTTAAATACTTCTCAAGAAAAAATTGTTTTTAGTAAAAGTAAAAATCC
AATGCTTGTTTTAGCAGGGCCTGGAAGTGGTAAAACAAGAGTTATAATTGCAAAAATTGTTTATTTAATCAAATATATGA
ATATAGATCCCAATGAAATTTTAGCTTTAACTTTTACCAATAAAGCTGCAAATGAAATGAATGATAGGATAAATGATCTT
TTAAAATTTGACAAAAAACTTCATATTCAAACTTTTCATTCTTTTGGGTCTTGGCTTTTGAGAGTTTACTATAAGGATTT
TAACGAAAATTACGATTCAAATTTTACAATTTGGGATACTAATGATGTTGTTAAATTTGTTAAACAAATTGATCTTGCTC
CAAATCTTGAAATGGCAAAACATATTGCAGCTTTGATTTTAAAAGACAAAGAAAATTTTTTCTTAGAAAAATTTATTCAA
TTTACAGAAAAGGAATATGAGTATATTAAAATTTATGAGGAAGAGAAAGCTAAAAATAATGCTTTTGATTTCTCAGATCT
TATTATTAAGCCTATTTTAATGCTGAGGCAATCTAAATCTTTAAAAGAGTCTATTCAATCTAGATTTAAAGTTATTTTTG
TAGATGAATATCAAGATACAAATTATTCACAATTTTTATTTTTAAAAGAACTTTATTTAGATGGTATGTATTTTATGGTC
GTAGGAGATGAAGATCAGTCAATATATTCTTTTAGGGGAGCTAGAATTGAAAATATTCTTGAATTTGAAAAAACTTTTGA
CAATGTTATTAAATTTTATTTAGTGCAAAATTATCGTTCAAATTCAAATATAGTGGGCATTGCAAATGAGGTTATTTCAA
AAAATAAAAATAGATATGAAAAGCAAATAACAACTCAAAATAGTTCTAATAAAAGGATGAAATTTTTAGTTTTTCAAAGC
ACTTCAGATGAAGCTGAATATTTTTCTAATTTGCTTATTTCCAATGATATTAAGACAGCAATACTTTATAGATTTAATTC
TCAATCTTTTCATTTTGAAACATCTTTTTTAAAGAAGAATATCCCATACAAGGTTTTAGGATCAATTAAATTTTATGATA
GAGAGGAAATAAAAGATATTATTTGTTTGCTTAGGCTTTTTATAAACAAGAAAGATAAAATATCTTTCTTGAGAATGATA
AACAAGCCTTCTAGAGGAATTGGAAAAACTACTCTAGACAAAATAATTAGTTCTTTAAACGATAAAGATGTTAATTTCAA
TTTGTTTTGTGCAAGTAAAAAGACTTTAGGTTTGCTTAAAAATAGAGCCAAAGAGTCTCTTTTATTATTTTTAAATGTTT
ATGAGGAGCTGGGTAAAAAACTTTTTGAAGATAATTATATTAATTTATCTGCTTTTATTGAGGATGTAGTAATTAGGTTT
GGTCTTTTAGATTATTATAGAAAATTTGATAAGGACGAAAAATTAAGAAATATTGATGAACTTATTAATAGTGGAATTGA
ATATTCAGGCACGTTTGAAGGTCTTGCTATATTTTTAGAAAATTCTTCACTTTCTCCTTTAATTTCTGGAGATTTTAAGT
CCAATATACTTTTGTCTTCAATTCACGGTGTTAAGGGGCTTGAATTTGATAGAGTTGTGATTTCTGGGCTTGAGAAAGGT
CTTTTACCTGCTGAAATTGAAGAATTAACAGAAGATAGACTTGAAGAGGAGAGAAGGCTTTTTTATGTKGCGATCACAAG
AGCTAAATCAGAGCTTATTGTTACCTTAAACTTAAGGCGAGCTTTTAGAGGTTCTTATAAGGGCACTTTGCCTTCTGTTT
TTTTCCAAGATATTGACAAAAACTCTTATGACATTATCTTTATCCCTGAGTATTTAAAAGAGAATTTTAATAATTTTTTT
ATTAATAACAAAAGGGATATTGGATTTAATATTGGAGATTATATAATTTATAATGGAGAAAAGGGAATAGTTGTTGATAG
CTGGTACCAAAGCAACTTGCAGTTTGTTAAAATTAGTTTGAGAAATGGTAAGAAAGCTATTTTGAGTCCTGAGTATATTA
AAAAAATTGTCAAAGTTTAG

Upstream 100 bases:

>100_bases
CTCCTTTTTTAAAAGAGCAGGGAAAAGAAATTTTTGCTGTTCAATTTTTTTGGGTAAAGACCCCCTCAGGGTTTGATCTT
AAGGTTTCTGAAGTTTATTA

Downstream 100 bases:

>100_bases
AGGTTTATTTTGAAAGATATACATTTAAAAAATAGCTTAAAATTAAGCTTAGTGACACTTAGTAGAGAGAGTGAAGATAA
ATTTATTTCAAAATTTGAGA

Product: DNA helicase UvrD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MMDKIKKFFSSLNTSQEKIVFSKSKNPMLVLAGPGSGKTRVIIAKIVYLIKYMNIDPNEILALTFTNKAANEMNDRINDL
LKFDKKLHIQTFHSFGSWLLRVYYKDFNENYDSNFTIWDTNDVVKFVKQIDLAPNLEMAKHIAALILKDKENFFLEKFIQ
FTEKEYEYIKIYEEEKAKNNAFDFSDLIIKPILMLRQSKSLKESIQSRFKVIFVDEYQDTNYSQFLFLKELYLDGMYFMV
VGDEDQSIYSFRGARIENILEFEKTFDNVIKFYLVQNYRSNSNIVGIANEVISKNKNRYEKQITTQNSSNKRMKFLVFQS
TSDEAEYFSNLLISNDIKTAILYRFNSQSFHFETSFLKKNIPYKVLGSIKFYDREEIKDIICLLRLFINKKDKISFLRMI
NKPSRGIGKTTLDKIISSLNDKDVNFNLFCASKKTLGLLKNRAKESLLLFLNVYEELGKKLFEDNYINLSAFIEDVVIRF
GLLDYYRKFDKDEKLRNIDELINSGIEYSGTFEGLAIFLENSSLSPLISGDFKSNILLSSIHGVKGLEFDRVVISGLEKG
LLPAEIEELTEDRLEEERRLFYVAITRAKSELIVTLNLRRAFRGSYKGTLPSVFFQDIDKNSYDIIFIPEYLKENFNNFF
INNKRDIGFNIGDYIIYNGEKGIVVDSWYQSNLQFVKISLRNGKKAILSPEYIKKIVKV

Sequences:

>Translated_699_residues
MMDKIKKFFSSLNTSQEKIVFSKSKNPMLVLAGPGSGKTRVIIAKIVYLIKYMNIDPNEILALTFTNKAANEMNDRINDL
LKFDKKLHIQTFHSFGSWLLRVYYKDFNENYDSNFTIWDTNDVVKFVKQIDLAPNLEMAKHIAALILKDKENFFLEKFIQ
FTEKEYEYIKIYEEEKAKNNAFDFSDLIIKPILMLRQSKSLKESIQSRFKVIFVDEYQDTNYSQFLFLKELYLDGMYFMV
VGDEDQSIYSFRGARIENILEFEKTFDNVIKFYLVQNYRSNSNIVGIANEVISKNKNRYEKQITTQNSSNKRMKFLVFQS
TSDEAEYFSNLLISNDIKTAILYRFNSQSFHFETSFLKKNIPYKVLGSIKFYDREEIKDIICLLRLFINKKDKISFLRMI
NKPSRGIGKTTLDKIISSLNDKDVNFNLFCASKKTLGLLKNRAKESLLLFLNVYEELGKKLFEDNYINLSAFIEDVVIRF
GLLDYYRKFDKDEKLRNIDELINSGIEYSGTFEGLAIFLENSSLSPLISGDFKSNILLSSIHGVKGLEFDRVVISGLEKG
LLPAEIEELTEDRLEEERRLFYVAITRAKSELIVTLNLRRAFRGSYKGTLPSVFFQDIDKNSYDIIFIPEYLKENFNNFF
INNKRDIGFNIGDYIIYNGEKGIVVDSWYQSNLQFVKISLRNGKKAILSPEYIKKIVKV
>Mature_699_residues
MMDKIKKFFSSLNTSQEKIVFSKSKNPMLVLAGPGSGKTRVIIAKIVYLIKYMNIDPNEILALTFTNKAANEMNDRINDL
LKFDKKLHIQTFHSFGSWLLRVYYKDFNENYDSNFTIWDTNDVVKFVKQIDLAPNLEMAKHIAALILKDKENFFLEKFIQ
FTEKEYEYIKIYEEEKAKNNAFDFSDLIIKPILMLRQSKSLKESIQSRFKVIFVDEYQDTNYSQFLFLKELYLDGMYFMV
VGDEDQSIYSFRGARIENILEFEKTFDNVIKFYLVQNYRSNSNIVGIANEVISKNKNRYEKQITTQNSSNKRMKFLVFQS
TSDEAEYFSNLLISNDIKTAILYRFNSQSFHFETSFLKKNIPYKVLGSIKFYDREEIKDIICLLRLFINKKDKISFLRMI
NKPSRGIGKTTLDKIISSLNDKDVNFNLFCASKKTLGLLKNRAKESLLLFLNVYEELGKKLFEDNYINLSAFIEDVVIRF
GLLDYYRKFDKDEKLRNIDELINSGIEYSGTFEGLAIFLENSSLSPLISGDFKSNILLSSIHGVKGLEFDRVVISGLEKG
LLPAEIEELTEDRLEEERRLFYVAITRAKSELIVTLNLRRAFRGSYKGTLPSVFFQDIDKNSYDIIFIPEYLKENFNNFF
INNKRDIGFNIGDYIIYNGEKGIVVDSWYQSNLQFVKISLRNGKKAILSPEYIKKIVKV

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=698, Percent_Identity=30.945558739255, Blast_Score=321, Evalue=1e-88,
Organism=Escherichia coli, GI48994965, Length=672, Percent_Identity=31.8452380952381, Blast_Score=271, Evalue=1e-73,
Organism=Escherichia coli, GI1787196, Length=157, Percent_Identity=29.2993630573248, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322369, Length=516, Percent_Identity=29.6511627906977, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324477, Length=406, Percent_Identity=25.8620689655172, Blast_Score=87, Evalue=6e-18,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 81731; Mature: 81731

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMDKIKKFFSSLNTSQEKIVFSKSKNPMLVLAGPGSGKTRVIIAKIVYLIKYMNIDPNEI
CHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCE
LALTFTNKAANEMNDRINDLLKFDKKLHIQTFHSFGSWLLRVYYKDFNENYDSNFTIWDT
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
NDVVKFVKQIDLAPNLEMAKHIAALILKDKENFFLEKFIQFTEKEYEYIKIYEEEKAKNN
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEHHCCCCC
AFDFSDLIIKPILMLRQSKSLKESIQSRFKVIFVDEYQDTNYSQFLFLKELYLDGMYFMV
CCCHHHHHHHHHHHHHCCHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
VGDEDQSIYSFRGARIENILEFEKTFDNVIKFYLVQNYRSNSNIVGIANEVISKNKNRYE
ECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCHHHH
KQITTQNSSNKRMKFLVFQSTSDEAEYFSNLLISNDIKTAILYRFNSQSFHFETSFLKKN
HHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEHHHHHCC
IPYKVLGSIKFYDREEIKDIICLLRLFINKKDKISFLRMINKPSRGIGKTTLDKIISSLN
CCCEEECCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCC
DKDVNFNLFCASKKTLGLLKNRAKESLLLFLNVYEELGKKLFEDNYINLSAFIEDVVIRF
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
GLLDYYRKFDKDEKLRNIDELINSGIEYSGTFEGLAIFLENSSLSPLISGDFKSNILLSS
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEECCCCCCCCHHHH
IHGVKGLEFDRVVISGLEKGLLPAEIEELTEDRLEEERRLFYVAITRAKSELIVTLNLRR
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCEEEEEEEEHH
AFRGSYKGTLPSVFFQDIDKNSYDIIFIPEYLKENFNNFFINNKRDIGFNIGDYIIYNGE
HHCCCCCCCCHHHHHHHCCCCCCEEEEECHHHHCCCCCEEECCCCCCCCCCCCEEEEECC
KGIVVDSWYQSNLQFVKISLRNGKKAILSPEYIKKIVKV
CCEEEECHHHCCEEEEEEEECCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MMDKIKKFFSSLNTSQEKIVFSKSKNPMLVLAGPGSGKTRVIIAKIVYLIKYMNIDPNEI
CHHHHHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCE
LALTFTNKAANEMNDRINDLLKFDKKLHIQTFHSFGSWLLRVYYKDFNENYDSNFTIWDT
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
NDVVKFVKQIDLAPNLEMAKHIAALILKDKENFFLEKFIQFTEKEYEYIKIYEEEKAKNN
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEHHCCCCC
AFDFSDLIIKPILMLRQSKSLKESIQSRFKVIFVDEYQDTNYSQFLFLKELYLDGMYFMV
CCCHHHHHHHHHHHHHCCHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
VGDEDQSIYSFRGARIENILEFEKTFDNVIKFYLVQNYRSNSNIVGIANEVISKNKNRYE
ECCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHCCCHHHH
KQITTQNSSNKRMKFLVFQSTSDEAEYFSNLLISNDIKTAILYRFNSQSFHFETSFLKKN
HHHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEEECCCCEEEEHHHHHCC
IPYKVLGSIKFYDREEIKDIICLLRLFINKKDKISFLRMINKPSRGIGKTTLDKIISSLN
CCCEEECCEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCC
DKDVNFNLFCASKKTLGLLKNRAKESLLLFLNVYEELGKKLFEDNYINLSAFIEDVVIRF
CCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
GLLDYYRKFDKDEKLRNIDELINSGIEYSGTFEGLAIFLENSSLSPLISGDFKSNILLSS
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCEECCCCCCCCHHHH
IHGVKGLEFDRVVISGLEKGLLPAEIEELTEDRLEEERRLFYVAITRAKSELIVTLNLRR
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEEEEEEEEECCCEEEEEEEEHH
AFRGSYKGTLPSVFFQDIDKNSYDIIFIPEYLKENFNNFFINNKRDIGFNIGDYIIYNGE
HHCCCCCCCCHHHHHHHCCCCCCEEEEECHHHHCCCCCEEECCCCCCCCCCCCEEEEECC
KGIVVDSWYQSNLQFVKISLRNGKKAILSPEYIKKIVKV
CCEEEECHHHCCEEEEEEEECCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA