Click here to switch to the map view.

The map label for this gene is slt [H]

Identifier: 15594604

GI number: 15594604

Start: 270488

End: 272701

Strand: Reverse

Name: slt [H]

Synonym: BB0259

Alternate gene names: 15594604

Gene position: 272701-270488 (Counterclockwise)

Preceding gene: 15594605

Following gene: 15594603

Centisome position: 29.94

GC content: 26.65

Gene sequence:

>2214_bases
TTGTTTCATATTACTTTGAATATGATAAAAATAGAATTAAGAGATATGAAAAAGATCAATATGTTTAATAGAAGTTCTTG
TGTATTACAAAATTTTCTTTTTCTTTTTTTATTTTTAAGTTTAGTTTCTTGCTTTGCAAAAAAAGAAATCTCAGGCAATA
ATTTTATTAAGGCGCATTCAAAAGAGTTTGATTTAAATAATTTAAATTGGTTATGGAATTTTGATTATACAAAAAAAAAT
TTTGATAAGCATTTTAACATAGATCCAAGTTCTTACATATATGTTGCTTATTTATTTAAAAAAATAGGATTTGAAGAGAA
ATTTGTAGAGTATATGAAAAAGGCCATAGCTAATGGAGATAGCATTGCATCCCAGTTTGCTGGGATTAAGCTTATTGAAT
ATTTTAACTCAGCAAAAGAGTATTTTGCATCTGAATTGATTGGAGAGAAGCTTTATAAAAAATACGAAAATAATAAATTT
ATTATACTGGGGTACTTTAAAAGTCTTTATTGGCAAAAGAAAAACGATAAGGCACTTAGTCTTTTAAATAAGCTTGATAA
GATGAAATTTTCTGATTATCAGGAAAATGAAAATATTTTATTAAAAGCAGTTCTTTACCTTAATCTTTCTAATGTAAGTG
AGTCAAAAATTTATTTTAATGAGCTTTTTGAGAACTTACCTGCAAATTATTTACATGTAAGAGCTTATGATTATTTTATT
ATTGAAAATAAGTCTAGGTATTTTGGTGCAAATTTTTTAAATCTTGTTAGATTTAAGTATGAAGTGGCAAATGGCAATTT
TAATGGTGCAATAAATATATTAAATAAAAATGGTTTAAATGATTATTATGACAATAACATTGTATTAAGTGATGTTTATA
AGGCTTTTATTAGTTCTGGCAAAGTTTCAAATGCTTTAACATTTTTTAGTAAAATAAAGAGCAAATATAAAAATTATTAT
TTAGGTATTCTAAACCTTAGAGAGAAAAATAATTTAGGACTTCTTCTTTTAAAAGAATATCTTGAAGGTTTAGATCTTAA
CAATGAGATTAACAGGCTTGATTTGCTTAATACTGCTTTTAGCAATTTAATTTTTACTAAGAGCGCAAGGGATTATTTTG
CCGAAAGTTTACCCAAGTTTTATACCGAGGGCGATAAAAAAAATTCTACTTTTATTAAGATTTTAGAAGAGTATATTTTG
GAATCAATTCAGCTTGAAGACTATGGCAATCTTTATAAGCTTTATTCTAATGCTCAAAAAGTTATTTCTAATTCTGTTTT
GTCTAAGCTTGCTTTTATTAATGCAAGGCTTATATATCATAAATTAATTAAACCTAACGTAAGCGGAGAATACAAGAGTC
TTTTGCATTCTGCTGTTAATTATGATAAATGGTCTTATTCTTCATTTATGAGTAGGTACTTATTAGATCAAAATATTGAT
GAATTTTTTACAGGTGGGTCTGATATTAAGTATGAGCAATCCGATTATGAGATTTTTTTGGAAGGGTTTTTAAAATTCAA
TCTTTGTAATTATGTTAGAGGGTTTATTTCTGAGGATTTTAGGAATGGATATAAATTTTCACTTGATTTTTATCGAAAAG
TATACGATGAACTTTTAAAGAGTGAAAATTATTACGATGCAACTCTTGTGATTAATTATCTTGTAAATCAAGATGAATCT
GCTTTAATGGAGAATGACTATAAAAGACTTTATCCTTATTTGTATGGATCTTTGATAGAATATTGGGCTAAAAGGAGAGG
GCTTGAAGCTAGTGTTGTATTTTCTTTAATAAAAGCAGAGAGTAGCTTTGAAAAAAATGCTGTCTCAAAACCGGGTGCTG
TTGGCCTTATGCAGGTTATGCCATCAACAGCAAATGATATTTCTAAAGAACTTAAGTATTTTAACTATGATTTAAAGATT
CCAAAAGATAATATAATAATTGGAACATATTATTTAAAAAAAAGAATATCTACAACTGGCAGTCTTTATAAGGCTCTTGC
GTCTTATAATGGGGGTATTGGTAATGTTAGAAAGTGGGAGAAAAGTTATGGACATTTGTCAAAAGAGCTTTTTATTGAGG
CAATTCCCTTTAGTCAAACTAGGAATTATATTAAAAAAATATTAGTTTATTCGGTATTTTATGATGCTTTGTATGAAAAG
AAGGGAATAGATTCAGTAATAGTTAAAATTATGGGCGAATTCCCCAAAAATTAA

Upstream 100 bases:

>100_bases
GTTCATTCTCCTATGTTATATTTGAGTCTTATTTCTCTTTTTCTTTTGATATTTTTTTCTTTGTTTTTAGTTTTTTCAAA
AATTTTAAAACCTAGAGATT

Downstream 100 bases:

>100_bases
TATTGGAGCATTTAATGACAAATATTTTAAGTGCAATTATTTTGGGCATTATTCAAGGCATTACAGAGTTTTTACCAATA
TCTAGTTCGGGACATTTATT

Product: hypothetical protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 737; Mature: 737

Protein sequence:

>737_residues
MFHITLNMIKIELRDMKKINMFNRSSCVLQNFLFLFLFLSLVSCFAKKEISGNNFIKAHSKEFDLNNLNWLWNFDYTKKN
FDKHFNIDPSSYIYVAYLFKKIGFEEKFVEYMKKAIANGDSIASQFAGIKLIEYFNSAKEYFASELIGEKLYKKYENNKF
IILGYFKSLYWQKKNDKALSLLNKLDKMKFSDYQENENILLKAVLYLNLSNVSESKIYFNELFENLPANYLHVRAYDYFI
IENKSRYFGANFLNLVRFKYEVANGNFNGAINILNKNGLNDYYDNNIVLSDVYKAFISSGKVSNALTFFSKIKSKYKNYY
LGILNLREKNNLGLLLLKEYLEGLDLNNEINRLDLLNTAFSNLIFTKSARDYFAESLPKFYTEGDKKNSTFIKILEEYIL
ESIQLEDYGNLYKLYSNAQKVISNSVLSKLAFINARLIYHKLIKPNVSGEYKSLLHSAVNYDKWSYSSFMSRYLLDQNID
EFFTGGSDIKYEQSDYEIFLEGFLKFNLCNYVRGFISEDFRNGYKFSLDFYRKVYDELLKSENYYDATLVINYLVNQDES
ALMENDYKRLYPYLYGSLIEYWAKRRGLEASVVFSLIKAESSFEKNAVSKPGAVGLMQVMPSTANDISKELKYFNYDLKI
PKDNIIIGTYYLKKRISTTGSLYKALASYNGGIGNVRKWEKSYGHLSKELFIEAIPFSQTRNYIKKILVYSVFYDALYEK
KGIDSVIVKIMGEFPKN

Sequences:

>Translated_737_residues
MFHITLNMIKIELRDMKKINMFNRSSCVLQNFLFLFLFLSLVSCFAKKEISGNNFIKAHSKEFDLNNLNWLWNFDYTKKN
FDKHFNIDPSSYIYVAYLFKKIGFEEKFVEYMKKAIANGDSIASQFAGIKLIEYFNSAKEYFASELIGEKLYKKYENNKF
IILGYFKSLYWQKKNDKALSLLNKLDKMKFSDYQENENILLKAVLYLNLSNVSESKIYFNELFENLPANYLHVRAYDYFI
IENKSRYFGANFLNLVRFKYEVANGNFNGAINILNKNGLNDYYDNNIVLSDVYKAFISSGKVSNALTFFSKIKSKYKNYY
LGILNLREKNNLGLLLLKEYLEGLDLNNEINRLDLLNTAFSNLIFTKSARDYFAESLPKFYTEGDKKNSTFIKILEEYIL
ESIQLEDYGNLYKLYSNAQKVISNSVLSKLAFINARLIYHKLIKPNVSGEYKSLLHSAVNYDKWSYSSFMSRYLLDQNID
EFFTGGSDIKYEQSDYEIFLEGFLKFNLCNYVRGFISEDFRNGYKFSLDFYRKVYDELLKSENYYDATLVINYLVNQDES
ALMENDYKRLYPYLYGSLIEYWAKRRGLEASVVFSLIKAESSFEKNAVSKPGAVGLMQVMPSTANDISKELKYFNYDLKI
PKDNIIIGTYYLKKRISTTGSLYKALASYNGGIGNVRKWEKSYGHLSKELFIEAIPFSQTRNYIKKILVYSVFYDALYEK
KGIDSVIVKIMGEFPKN
>Mature_737_residues
MFHITLNMIKIELRDMKKINMFNRSSCVLQNFLFLFLFLSLVSCFAKKEISGNNFIKAHSKEFDLNNLNWLWNFDYTKKN
FDKHFNIDPSSYIYVAYLFKKIGFEEKFVEYMKKAIANGDSIASQFAGIKLIEYFNSAKEYFASELIGEKLYKKYENNKF
IILGYFKSLYWQKKNDKALSLLNKLDKMKFSDYQENENILLKAVLYLNLSNVSESKIYFNELFENLPANYLHVRAYDYFI
IENKSRYFGANFLNLVRFKYEVANGNFNGAINILNKNGLNDYYDNNIVLSDVYKAFISSGKVSNALTFFSKIKSKYKNYY
LGILNLREKNNLGLLLLKEYLEGLDLNNEINRLDLLNTAFSNLIFTKSARDYFAESLPKFYTEGDKKNSTFIKILEEYIL
ESIQLEDYGNLYKLYSNAQKVISNSVLSKLAFINARLIYHKLIKPNVSGEYKSLLHSAVNYDKWSYSSFMSRYLLDQNID
EFFTGGSDIKYEQSDYEIFLEGFLKFNLCNYVRGFISEDFRNGYKFSLDFYRKVYDELLKSENYYDATLVINYLVNQDES
ALMENDYKRLYPYLYGSLIEYWAKRRGLEASVVFSLIKAESSFEKNAVSKPGAVGLMQVMPSTANDISKELKYFNYDLKI
PKDNIIIGTYYLKKRISTTGSLYKALASYNGGIGNVRKWEKSYGHLSKELFIEAIPFSQTRNYIKKILVYSVFYDALYEK
KGIDSVIVKIMGEFPKN

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=157, Percent_Identity=33.7579617834395, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 86338; Mature: 86338

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHITLNMIKIELRDMKKINMFNRSSCVLQNFLFLFLFLSLVSCFAKKEISGNNFIKAHS
CEEEEEEEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
KEFDLNNLNWLWNFDYTKKNFDKHFNIDPSSYIYVAYLFKKIGFEEKFVEYMKKAIANGD
CCCCCCCCCEEEECCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
SIASQFAGIKLIEYFNSAKEYFASELIGEKLYKKYENNKFIILGYFKSLYWQKKNDKALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHH
LLNKLDKMKFSDYQENENILLKAVLYLNLSNVSESKIYFNELFENLPANYLHVRAYDYFI
HHHHHHHCCCCCCCCCCCCEEEHEEHHEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEEE
IENKSRYFGANFLNLVRFKYEVANGNFNGAINILNKNGLNDYYDNNIVLSDVYKAFISSG
EECCCCCCCHHHHHHHHHEEEECCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHCCC
KVSNALTFFSKIKSKYKNYYLGILNLREKNNLGLLLLKEYLEGLDLNNEINRLDLLNTAF
CHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
SNLIFTKSARDYFAESLPKFYTEGDKKNSTFIKILEEYILESIQLEDYGNLYKLYSNAQK
HHHEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHH
VISNSVLSKLAFINARLIYHKLIKPNVSGEYKSLLHSAVNYDKWSYSSFMSRYLLDQNID
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
EFFTGGSDIKYEQSDYEIFLEGFLKFNLCNYVRGFISEDFRNGYKFSLDFYRKVYDELLK
HHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHC
SENYYDATLVINYLVNQDESALMENDYKRLYPYLYGSLIEYWAKRRGLEASVVFSLIKAE
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SSFEKNAVSKPGAVGLMQVMPSTANDISKELKYFNYDLKIPKDNIIIGTYYLKKRISTTG
HHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCEEEEHHHHHHHHHHHH
SLYKALASYNGGIGNVRKWEKSYGHLSKELFIEAIPFSQTRNYIKKILVYSVFYDALYEK
HHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
KGIDSVIVKIMGEFPKN
CCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFHITLNMIKIELRDMKKINMFNRSSCVLQNFLFLFLFLSLVSCFAKKEISGNNFIKAHS
CEEEEEEEEEEEHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECC
KEFDLNNLNWLWNFDYTKKNFDKHFNIDPSSYIYVAYLFKKIGFEEKFVEYMKKAIANGD
CCCCCCCCCEEEECCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHCCCH
SIASQFAGIKLIEYFNSAKEYFASELIGEKLYKKYENNKFIILGYFKSLYWQKKNDKALS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCCHHHHH
LLNKLDKMKFSDYQENENILLKAVLYLNLSNVSESKIYFNELFENLPANYLHVRAYDYFI
HHHHHHHCCCCCCCCCCCCEEEHEEHHEECCCCHHHHHHHHHHHHCCCCEEEEEEEEEEE
IENKSRYFGANFLNLVRFKYEVANGNFNGAINILNKNGLNDYYDNNIVLSDVYKAFISSG
EECCCCCCCHHHHHHHHHEEEECCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHCCC
KVSNALTFFSKIKSKYKNYYLGILNLREKNNLGLLLLKEYLEGLDLNNEINRLDLLNTAF
CHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHH
SNLIFTKSARDYFAESLPKFYTEGDKKNSTFIKILEEYILESIQLEDYGNLYKLYSNAQK
HHHEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHH
VISNSVLSKLAFINARLIYHKLIKPNVSGEYKSLLHSAVNYDKWSYSSFMSRYLLDQNID
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHH
EFFTGGSDIKYEQSDYEIFLEGFLKFNLCNYVRGFISEDFRNGYKFSLDFYRKVYDELLK
HHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHC
SENYYDATLVINYLVNQDESALMENDYKRLYPYLYGSLIEYWAKRRGLEASVVFSLIKAE
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
SSFEKNAVSKPGAVGLMQVMPSTANDISKELKYFNYDLKIPKDNIIIGTYYLKKRISTTG
HHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCEEEEHHHHHHHHHHHH
SLYKALASYNGGIGNVRKWEKSYGHLSKELFIEAIPFSQTRNYIKKILVYSVFYDALYEK
HHHHHHHHCCCCCCCHHHHHHHHHCHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHH
KGIDSVIVKIMGEFPKN
CCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]