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The map label for this gene is asnC

Identifier: 15594447

GI number: 15594447

Start: 98805

End: 100205

Strand: Direct

Name: asnC

Synonym: BB0101

Alternate gene names: 15594447

Gene position: 98805-100205 (Clockwise)

Preceding gene: 15594432

Following gene: 15594448

Centisome position: 10.85

GC content: 30.19

Gene sequence:

>1401_bases
ATGATAAATAAAATGTTTGCAAGCATTAAAGATATTTTAAAAAACCCAATTTTAAACAGCAATGTTACAATAAACGGCTG
GATTAGAACCAAAAGAAGTAATGGCAAAATTGGATTTATAGAAATTAATGATGGTTCAACGCTTAAAGGAATTCAAGCTG
TAATAAACGAAGAAGAAAATCAATTTAGTGAAAAAGATCTAAAAAAATTAACAACAGGAACAAGTATATCGTTAACGGGC
CTATTAGTAGAAAGTCCTGCAAAAGGGCAAAATTATGAAATAAAAACACATAGTTTTAATGTAATCGGGGAAACAGACCC
AGAAACTTACCCATTGCAAAAGAAAAGACATTCTTTTGAATTTTTAAGAGAAATACCCCATTTAAGAATACGAACCAACA
CATTTGGAGCTATTGCTAGGGTAAGAAACAAAATTTCATACAAAATTCATGAATATTTCCAAAAAAATGGTTTTTTTTAT
ATTAATACACCAATAATTACATCAAATGATGGGGAAGGTGCTGGAGAAATGTTTAGGGTTTCCACACTAAAATTTAATAA
ACTAAACAATGCACTTAGCAATATTGATTTTAAAGACGATTTTTTTGGAAAAGAAGCTTTTCTCTCTGTCACTGGTCAAT
TGCATGGGGAAGCATATGCAATGGCTTTATCTAAAATATATACATTCGGACCAACATTTAGAGCAGAAAATTCTAACACC
ACACGCCACGCTTCAGAATTTTGGATGATAGAACCAGAAATGGCTTTTTATAAGCTTAACGACAATATTGCCCTAGCAGA
AGATCTCTTGAAATATCTTTTAAGTTCAATTTTAAACGAATGCTCACAAGATATGGATTTTTTAGAAAATTACATTGAAA
AAGGTTTAATTAAAAAACTAGAAAATGTAATAAATTCAAATTTTGAGGTTATTACCTATACTAAAGCAATTGAAATTCTT
GAAAACTCAAAAAAAAATTTTGAAATAAAACCTTACTGGGGAATAGATTTGCAAACAGATCACGAAAGATACCTAACAGA
AGAGACTTTTAAAAAACCGGTAGTGGTCATTGATTATCCAAAAAATTTCAAAGCATTTTACATGAAAGCAAATAAAGACA
ATAAAACTGTTAAAGGAATGGACATACTTGTTCCAAAAATTGGAGAGATTATAGGGGGAAGCGAAAGAGAAGATGACCTT
CAAAAATTAGAAAATAGAATAAAAGAATTAAACTTAAACATTGAACATCTAAACTGGTATCTTGATCTAAGAAGATTTGG
CTCGGCTCCTCATTCTGGCTTTGGACTTGGACTTGAAAGATTGGTGCAATACTCAACAGGAATATCTAATATAAGAGATT
CAATACCATTCCCAAGGACTCCTAAAAATCTTTATTTTTAA

Upstream 100 bases:

>100_bases
CAAGAATTAAAATAATAAAAATTGTTAAATGTTTTTTTTAAAAAAAGTTTTAAGCTATAATAAAATTGATTTAACAATAT
TAATAAGTTAAGTATTTAGG

Downstream 100 bases:

>100_bases
TCAAAAGAAAGGAAACAAAAAGATGAAATTACCAAAACTTTACAAATTAATACTACTCTTTCTTTTTACAACAAGATTGT
TTTCAGTAAAAGATGAAAAA

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MINKMFASIKDILKNPILNSNVTINGWIRTKRSNGKIGFIEINDGSTLKGIQAVINEEENQFSEKDLKKLTTGTSISLTG
LLVESPAKGQNYEIKTHSFNVIGETDPETYPLQKKRHSFEFLREIPHLRIRTNTFGAIARVRNKISYKIHEYFQKNGFFY
INTPIITSNDGEGAGEMFRVSTLKFNKLNNALSNIDFKDDFFGKEAFLSVTGQLHGEAYAMALSKIYTFGPTFRAENSNT
TRHASEFWMIEPEMAFYKLNDNIALAEDLLKYLLSSILNECSQDMDFLENYIEKGLIKKLENVINSNFEVITYTKAIEIL
ENSKKNFEIKPYWGIDLQTDHERYLTEETFKKPVVVIDYPKNFKAFYMKANKDNKTVKGMDILVPKIGEIIGGSEREDDL
QKLENRIKELNLNIEHLNWYLDLRRFGSAPHSGFGLGLERLVQYSTGISNIRDSIPFPRTPKNLYF

Sequences:

>Translated_466_residues
MINKMFASIKDILKNPILNSNVTINGWIRTKRSNGKIGFIEINDGSTLKGIQAVINEEENQFSEKDLKKLTTGTSISLTG
LLVESPAKGQNYEIKTHSFNVIGETDPETYPLQKKRHSFEFLREIPHLRIRTNTFGAIARVRNKISYKIHEYFQKNGFFY
INTPIITSNDGEGAGEMFRVSTLKFNKLNNALSNIDFKDDFFGKEAFLSVTGQLHGEAYAMALSKIYTFGPTFRAENSNT
TRHASEFWMIEPEMAFYKLNDNIALAEDLLKYLLSSILNECSQDMDFLENYIEKGLIKKLENVINSNFEVITYTKAIEIL
ENSKKNFEIKPYWGIDLQTDHERYLTEETFKKPVVVIDYPKNFKAFYMKANKDNKTVKGMDILVPKIGEIIGGSEREDDL
QKLENRIKELNLNIEHLNWYLDLRRFGSAPHSGFGLGLERLVQYSTGISNIRDSIPFPRTPKNLYF
>Mature_466_residues
MINKMFASIKDILKNPILNSNVTINGWIRTKRSNGKIGFIEINDGSTLKGIQAVINEEENQFSEKDLKKLTTGTSISLTG
LLVESPAKGQNYEIKTHSFNVIGETDPETYPLQKKRHSFEFLREIPHLRIRTNTFGAIARVRNKISYKIHEYFQKNGFFY
INTPIITSNDGEGAGEMFRVSTLKFNKLNNALSNIDFKDDFFGKEAFLSVTGQLHGEAYAMALSKIYTFGPTFRAENSNT
TRHASEFWMIEPEMAFYKLNDNIALAEDLLKYLLSSILNECSQDMDFLENYIEKGLIKKLENVINSNFEVITYTKAIEIL
ENSKKNFEIKPYWGIDLQTDHERYLTEETFKKPVVVIDYPKNFKAFYMKANKDNKTVKGMDILVPKIGEIIGGSEREDDL
QKLENRIKELNLNIEHLNWYLDLRRFGSAPHSGFGLGLERLVQYSTGISNIRDSIPFPRTPKNLYF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI226491502, Length=447, Percent_Identity=43.4004474272931, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI4758762, Length=456, Percent_Identity=25.8771929824561, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI45439306, Length=342, Percent_Identity=28.0701754385965, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1787161, Length=462, Percent_Identity=53.8961038961039, Blast_Score=534, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI17556372, Length=441, Percent_Identity=32.6530612244898, Blast_Score=202, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI71984122, Length=453, Percent_Identity=27.8145695364238, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17551876, Length=489, Percent_Identity=25.7668711656442, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319872, Length=469, Percent_Identity=40.9381663113006, Blast_Score=331, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6321807, Length=460, Percent_Identity=28.0434782608696, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323011, Length=351, Percent_Identity=26.7806267806268, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI19922416, Length=463, Percent_Identity=35.4211663066955, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI19921528, Length=449, Percent_Identity=26.0579064587973, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17136276, Length=343, Percent_Identity=25.6559766763848, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYN_BORBU (O51128)

Other databases:

- EMBL:   AE000783
- PIR:   E70112
- RefSeq:   NP_212235.1
- ProteinModelPortal:   O51128
- SMR:   O51128
- EnsemblBacteria:   EBBORT00000008535
- GeneID:   1194937
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0101
- NMPDR:   fig|224326.1.peg.485
- TIGR:   BB_0101
- GeneTree:   EBGT00050000007238
- HOGENOM:   HBG745843
- OMA:   EPEMAFC
- PhylomeDB:   O51128
- ProtClustDB:   PRK03932
- BioCyc:   BBUR224326:BB_0101-MONOMER
- BRENDA:   6.1.1.22
- GO:   GO:0005737
- HAMAP:   MF_00534
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00457

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.22

Molecular weight: Translated: 53601; Mature: 53601

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKMFASIKDILKNPILNSNVTINGWIRTKRSNGKIGFIEINDGSTLKGIQAVINEEEN
CCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHCCHHH
QFSEKDLKKLTTGTSISLTGLLVESPAKGQNYEIKTHSFNVIGETDPETYPLQKKRHSFE
HHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHH
FLREIPHLRIRTNTFGAIARVRNKISYKIHEYFQKNGFFYINTPIITSNDGEGAGEMFRV
HHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCCCEEEE
STLKFNKLNNALSNIDFKDDFFGKEAFLSVTGQLHGEAYAMALSKIYTFGPTFRAENSNT
EEEEHHHHHHHHHCCCCCCCCCCCHHEEEEHHHHCCHHHHHHHHHHHHCCCCEECCCCCC
TRHASEFWMIEPEMAFYKLNDNIALAEDLLKYLLSSILNECSQDMDFLENYIEKGLIKKL
CCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENVINSNFEVITYTKAIEILENSKKNFEIKPYWGIDLQTDHERYLTEETFKKPVVVIDYP
HHHHCCCCEEEEHHHHHHHHHCCCCCEEECCEECCCCCCCHHHHHHHHHHCCCEEEEECC
KNFKAFYMKANKDNKTVKGMDILVPKIGEIIGGSEREDDLQKLENRIKELNLNIEHLNWY
CCCEEEEEEECCCCCEECCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHE
LDLRRFGSAPHSGFGLGLERLVQYSTGISNIRDSIPFPRTPKNLYF
EEHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MINKMFASIKDILKNPILNSNVTINGWIRTKRSNGKIGFIEINDGSTLKGIQAVINEEEN
CCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHCCHHH
QFSEKDLKKLTTGTSISLTGLLVESPAKGQNYEIKTHSFNVIGETDPETYPLQKKRHSFE
HHHHHHHHHHHCCCCEEEEEEEEECCCCCCCEEEEEEEEEEEECCCCCCCCCHHHHHHHH
FLREIPHLRIRTNTFGAIARVRNKISYKIHEYFQKNGFFYINTPIITSNDGEGAGEMFRV
HHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCEEECCCCCCCCCEEEE
STLKFNKLNNALSNIDFKDDFFGKEAFLSVTGQLHGEAYAMALSKIYTFGPTFRAENSNT
EEEEHHHHHHHHHCCCCCCCCCCCHHEEEEHHHHCCHHHHHHHHHHHHCCCCEECCCCCC
TRHASEFWMIEPEMAFYKLNDNIALAEDLLKYLLSSILNECSQDMDFLENYIEKGLIKKL
CCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENVINSNFEVITYTKAIEILENSKKNFEIKPYWGIDLQTDHERYLTEETFKKPVVVIDYP
HHHHCCCCEEEEHHHHHHHHHCCCCCEEECCEECCCCCCCHHHHHHHHHHCCCEEEEECC
KNFKAFYMKANKDNKTVKGMDILVPKIGEIIGGSEREDDLQKLENRIKELNLNIEHLNWY
CCCEEEEEEECCCCCEECCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHE
LDLRRFGSAPHSGFGLGLERLVQYSTGISNIRDSIPFPRTPKNLYF
EEHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685