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The map label for this gene is parE

Identifier: 15594382

GI number: 15594382

Start: 34037

End: 35836

Strand: Reverse

Name: parE

Synonym: BB0036

Alternate gene names: 15594382

Gene position: 35836-34037 (Counterclockwise)

Preceding gene: 15594383

Following gene: 15594381

Centisome position: 3.93

GC content: 29.39

Gene sequence:

>1800_bases
ATGAAGACTCAAAATTATGATGAAAGTAAAATAATCACTCTATCTTCTCTTGAACATATTAGATTAAGATCTGGTATGTA
TATTGGACGTTTGGGAGATGGCTCTAATATTGATGATGGTATTTATGTTTTAATTAAAGAGATAATAGACAATTCAATTG
ATGAGTTTATTATGGGTTACGGAAATGAAATTTTTATAAAAAAAGAAAATAATCTTATTTCTATTAGGGATTATGGAAGA
GGAATTCCTCTTGGAAAAGTTATTGAGAGTGTTTCAGTTATTAATACTGGAGCCAAGTACAATGACGATGTTTTTCAATT
TTCTGTAGGGCTTAATGGGGTTGGAACTAAGGCAGTTAATGCCTTAAGTTCAAAATTTTTAGTAAGATCAACAAGAAATG
GCAAATCTTTTGAGGCTTTGTTTTCTAAGGGAAAATTATTGGAATCTAGAGAAATAAAATCTTCTGATAAAGATGGTACT
TATGTTGAGTTTTTAGCAGATTCAGAAATATTCGGAAAATATTCTTATAGTGAAGATTTTCTCAAAAGAAGATTTTTCCA
TTATGCTTGTTTGAATAAGGGACTTATAATAAACTATAATGATCAAATTTTTGAATCTAAAAATGGTCTTTTAGATTTTT
TGAATTCAGAAATTAAAAGTGATGATTTGCTTTATGATATTGTTTACTATTCTAGTAAAACTTTAGAATTTGCTTTTTCC
CATACAAATAATTATGGGGAGACTTATTTTTCATTTGTTAATGGTCAATATACTAATGATGGAGGTACCCATCAGACTGG
TTTTAGAGAGGGCTTTGTAAGAGCTATTAACGATTTTCTTAAAAAAACATATTCCTCTACGGATATTAGGGAAGGGCTTG
TTGCAACTCTTTCTGTTAAAATTAAAGATCCAATATTTGAAAGTCAAACTAAGAATAAGCTTGGAAATATTGAAACTAGA
GGCAATGTTGCAAAGGAAGTGCAGAAGATAATTTCTGAAATTTTATATAAAGATAAAATACTTGCAAAATTGATTGAGAA
AAAAGTAGTTGACAATGAACGACTCAGAAAAGAATTAAGTAGCGTAAGAAAAGAAGCAAGAGAGAGAGCAAAAAAAATAT
CTTTTAAAATCCCTAAGCTTAAGGATTGCAAATTTCATTTTAATGACAGCAGCAAGCAGTCAGAACAAACTATGATCTTC
TTAACAGAGGGGGATTCTGCAACAGGTTCAATGGTATCTTGTAGAGATGTTTATACTCAGGCTATATTTTCTCTTAGAGG
AAAACCTCAAAATATGTTTGAAAAGAATAAATCTGAAATATACAAAAATGAAGAACTTTATAATATGATGGTAGCTCTTG
GCATTGAAGAATCAATTGAAAATTTAAGGTACAATAAGGTTGTAATAGCAACCGATGCAGATTTTGATGGATTTCATATT
AGAAATTTATTGTTAACTTTTTTCTTAACTTTTTTTGAGGATTTAATTTTAAATGGGCATATGTATATTTTAGAAACTCC
TCTTTTTAGGGTTAGGAACAAAAAAATCACGACCTATTGTTATTCTGAGGAAGAAAAGCAAAAAGCCATTCTTGAGCTTA
AGGGGGGATGTGAAGTAACAAGGTTTAAAGGCCTTGGTGAAATTTCTCCAAATGAATTTAAAGGCTTTATTGATATTAAT
AGCATTAAGCTTACAAAGGTGGACCTTTTTAATATTAAAGAAATAAAAGAGAAATTGGGTTTTTATATGGGGCAAAATAC
TCCTGAGAGGCGGAATTTTATTATGGAGAATTTGATTTAA

Upstream 100 bases:

>100_bases
TAAGTGAGTATGAAAAAGAGAATCTCACTAGTATTATTAGAGATCAAATAGTTAAAAAGCTTGAAACTATTAAAATTTAA
TTTATAAAGCAAGGATTTAG

Downstream 100 bases:

>100_bases
TGGATATTAGAACTGTACTTAAAGATAATTTTTTGCAATATTCATCTTATGTTATTAAAGATCGTGCTATTGCTAGTGTT
GTTGATGGGTTTAAACCAGT

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MKTQNYDESKIITLSSLEHIRLRSGMYIGRLGDGSNIDDGIYVLIKEIIDNSIDEFIMGYGNEIFIKKENNLISIRDYGR
GIPLGKVIESVSVINTGAKYNDDVFQFSVGLNGVGTKAVNALSSKFLVRSTRNGKSFEALFSKGKLLESREIKSSDKDGT
YVEFLADSEIFGKYSYSEDFLKRRFFHYACLNKGLIINYNDQIFESKNGLLDFLNSEIKSDDLLYDIVYYSSKTLEFAFS
HTNNYGETYFSFVNGQYTNDGGTHQTGFREGFVRAINDFLKKTYSSTDIREGLVATLSVKIKDPIFESQTKNKLGNIETR
GNVAKEVQKIISEILYKDKILAKLIEKKVVDNERLRKELSSVRKEARERAKKISFKIPKLKDCKFHFNDSSKQSEQTMIF
LTEGDSATGSMVSCRDVYTQAIFSLRGKPQNMFEKNKSEIYKNEELYNMMVALGIEESIENLRYNKVVIATDADFDGFHI
RNLLLTFFLTFFEDLILNGHMYILETPLFRVRNKKITTYCYSEEEKQKAILELKGGCEVTRFKGLGEISPNEFKGFIDIN
SIKLTKVDLFNIKEIKEKLGFYMGQNTPERRNFIMENLI

Sequences:

>Translated_599_residues
MKTQNYDESKIITLSSLEHIRLRSGMYIGRLGDGSNIDDGIYVLIKEIIDNSIDEFIMGYGNEIFIKKENNLISIRDYGR
GIPLGKVIESVSVINTGAKYNDDVFQFSVGLNGVGTKAVNALSSKFLVRSTRNGKSFEALFSKGKLLESREIKSSDKDGT
YVEFLADSEIFGKYSYSEDFLKRRFFHYACLNKGLIINYNDQIFESKNGLLDFLNSEIKSDDLLYDIVYYSSKTLEFAFS
HTNNYGETYFSFVNGQYTNDGGTHQTGFREGFVRAINDFLKKTYSSTDIREGLVATLSVKIKDPIFESQTKNKLGNIETR
GNVAKEVQKIISEILYKDKILAKLIEKKVVDNERLRKELSSVRKEARERAKKISFKIPKLKDCKFHFNDSSKQSEQTMIF
LTEGDSATGSMVSCRDVYTQAIFSLRGKPQNMFEKNKSEIYKNEELYNMMVALGIEESIENLRYNKVVIATDADFDGFHI
RNLLLTFFLTFFEDLILNGHMYILETPLFRVRNKKITTYCYSEEEKQKAILELKGGCEVTRFKGLGEISPNEFKGFIDIN
SIKLTKVDLFNIKEIKEKLGFYMGQNTPERRNFIMENLI
>Mature_599_residues
MKTQNYDESKIITLSSLEHIRLRSGMYIGRLGDGSNIDDGIYVLIKEIIDNSIDEFIMGYGNEIFIKKENNLISIRDYGR
GIPLGKVIESVSVINTGAKYNDDVFQFSVGLNGVGTKAVNALSSKFLVRSTRNGKSFEALFSKGKLLESREIKSSDKDGT
YVEFLADSEIFGKYSYSEDFLKRRFFHYACLNKGLIINYNDQIFESKNGLLDFLNSEIKSDDLLYDIVYYSSKTLEFAFS
HTNNYGETYFSFVNGQYTNDGGTHQTGFREGFVRAINDFLKKTYSSTDIREGLVATLSVKIKDPIFESQTKNKLGNIETR
GNVAKEVQKIISEILYKDKILAKLIEKKVVDNERLRKELSSVRKEARERAKKISFKIPKLKDCKFHFNDSSKQSEQTMIF
LTEGDSATGSMVSCRDVYTQAIFSLRGKPQNMFEKNKSEIYKNEELYNMMVALGIEESIENLRYNKVVIATDADFDGFHI
RNLLLTFFLTFFEDLILNGHMYILETPLFRVRNKKITTYCYSEEEKQKAILELKGGCEVTRFKGLGEISPNEFKGFIDIN
SIKLTKVDLFNIKEIKEKLGFYMGQNTPERRNFIMENLI

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family

Homologues:

Organism=Homo sapiens, GI19913408, Length=612, Percent_Identity=26.9607843137255, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI19913406, Length=612, Percent_Identity=26.797385620915, Blast_Score=129, Evalue=7e-30,
Organism=Escherichia coli, GI48994957, Length=559, Percent_Identity=31.8425760286225, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1789408, Length=636, Percent_Identity=29.2452830188679, Blast_Score=251, Evalue=8e-68,
Organism=Caenorhabditis elegans, GI17535065, Length=614, Percent_Identity=27.1986970684039, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI212645845, Length=540, Percent_Identity=26.6666666666667, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324241, Length=609, Percent_Identity=25.615763546798, Blast_Score=100, Evalue=7e-22,
Organism=Drosophila melanogaster, GI17136538, Length=605, Percent_Identity=26.9421487603306, Blast_Score=132, Evalue=7e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PARE_BORBU (Q59189)

Other databases:

- EMBL:   AE000783
- EMBL:   L32861
- PIR:   D70104
- RefSeq:   NP_212170.1
- ProteinModelPortal:   Q59189
- EnsemblBacteria:   EBBORT00000008389
- GeneID:   1194872
- GenomeReviews:   AE000783_GR
- KEGG:   bbu:BB0036
- NMPDR:   fig|224326.1.peg.420
- TIGR:   BB_0036
- GeneTree:   EBGT00050000007199
- HOGENOM:   HBG304889
- OMA:   VFESQTK
- ProtClustDB:   PRK05559
- BioCyc:   BBUR224326:BB_0036-MONOMER
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: 5.99.1.-

Molecular weight: Translated: 68775; Mature: 68775

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQNYDESKIITLSSLEHIRLRSGMYIGRLGDGSNIDDGIYVLIKEIIDNSIDEFIMGY
CCCCCCCCCEEEEECCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHCC
GNEIFIKKENNLISIRDYGRGIPLGKVIESVSVINTGAKYNDDVFQFSVGLNGVGTKAVN
CCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHH
ALSSKFLVRSTRNGKSFEALFSKGKLLESREIKSSDKDGTYVEFLADSEIFGKYSYSEDF
HHHHHHHEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCHHH
LKRRFFHYACLNKGLIINYNDQIFESKNGLLDFLNSEIKSDDLLYDIVYYSSKTLEFAFS
HHHHHHHHHHHCCCEEEECCHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEE
HTNNYGETYFSFVNGQYTNDGGTHQTGFREGFVRAINDFLKKTYSSTDIREGLVATLSVK
CCCCCCHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEEE
IKDPIFESQTKNKLGNIETRGNVAKEVQKIISEILYKDKILAKLIEKKVVDNERLRKELS
ECCCCHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SVRKEARERAKKISFKIPKLKDCKFHFNDSSKQSEQTMIFLTEGDSATGSMVSCRDVYTQ
HHHHHHHHHHHHCEECCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
AIFSLRGKPQNMFEKNKSEIYKNEELYNMMVALGIEESIENLRYNKVVIATDADFDGFHI
HHHHHCCCCHHHHHCCHHHHHCCHHHHHHHHHHCHHHHHHCCCCCEEEEEECCCCCCHHH
RNLLLTFFLTFFEDLILNGHMYILETPLFRVRNKKITTYCYSEEEKQKAILELKGGCEVT
HHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCEEEEEEECCHHHHHHHHEECCCCCEE
RFKGLGEISPNEFKGFIDINSIKLTKVDLFNIKEIKEKLGFYMGQNTPERRNFIMENLI
EECCCCCCCCCCCCCEEEECEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKTQNYDESKIITLSSLEHIRLRSGMYIGRLGDGSNIDDGIYVLIKEIIDNSIDEFIMGY
CCCCCCCCCEEEEECCHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHCC
GNEIFIKKENNLISIRDYGRGIPLGKVIESVSVINTGAKYNDDVFQFSVGLNGVGTKAVN
CCEEEEEECCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHH
ALSSKFLVRSTRNGKSFEALFSKGKLLESREIKSSDKDGTYVEFLADSEIFGKYSYSEDF
HHHHHHHEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHCCCCCCHHH
LKRRFFHYACLNKGLIINYNDQIFESKNGLLDFLNSEIKSDDLLYDIVYYSSKTLEFAFS
HHHHHHHHHHHCCCEEEECCHHHHHCCCHHHHHHHHHCCCCCCEEEEEEECCCEEEEEEE
HTNNYGETYFSFVNGQYTNDGGTHQTGFREGFVRAINDFLKKTYSSTDIREGLVATLSVK
CCCCCCHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEEE
IKDPIFESQTKNKLGNIETRGNVAKEVQKIISEILYKDKILAKLIEKKVVDNERLRKELS
ECCCCHHCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
SVRKEARERAKKISFKIPKLKDCKFHFNDSSKQSEQTMIFLTEGDSATGSMVSCRDVYTQ
HHHHHHHHHHHHCEECCCCCCCCEEEECCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHH
AIFSLRGKPQNMFEKNKSEIYKNEELYNMMVALGIEESIENLRYNKVVIATDADFDGFHI
HHHHHCCCCHHHHHCCHHHHHCCHHHHHHHHHHCHHHHHHCCCCCEEEEEECCCCCCHHH
RNLLLTFFLTFFEDLILNGHMYILETPLFRVRNKKITTYCYSEEEKQKAILELKGGCEVT
HHHHHHHHHHHHHHHHHCCCEEEEECCHHHHCCCEEEEEEECCHHHHHHHHEECCCCCEE
RFKGLGEISPNEFKGFIDINSIKLTKVDLFNIKEIKEKLGFYMGQNTPERRNFIMENLI
EECCCCCCCCCCCCCEEEECEEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685; 7812434