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The map label for this gene is mdh

Identifier: 154687044

GI number: 154687044

Start: 2741409

End: 2742347

Strand: Reverse

Name: mdh

Synonym: RBAM_026160

Alternate gene names: 154687044

Gene position: 2742347-2741409 (Counterclockwise)

Preceding gene: 154687045

Following gene: 154687043

Centisome position: 69.98

GC content: 47.82

Gene sequence:

>939_bases
ATGGGAAAAACACGCAGTAAAGTTTCTGTTATCGGAGCAGGATTTACGGGAGCGACAACAGCCTTTCTGACAGCTCAAAA
AGAGCTTGCGGACGTTGTGCTCGTTGATATTCCGCAATTAGAAAACCCGACAAAAGGAAAAGCGCTAGACATGCTCGAAG
CAAGTCCGGTTCAAGGATTTGACGCGAAAATCACAGGCACGTCCAACTACGAGGACACAGCCGGTTCTGATATCGTCGTC
ATCACAGCCGGAATTGCGAGAAAACCGGGCATGAGCCGTGATGATCTCGTATCTACAAACGAAAAAATCATGAGAAGCGT
CACACGTGAGATCGTGAAATATTCTCCTGACTGCATTATCGTTGTATTGACAAACCCTGTTGATGCGATGACATATGCGG
TGTACAAAGAATCAGGCTTCCCGAAAGAACGCGTCATCGGACAATCCGGAGTGCTTGACACTGCGCGATTCAGAACATTC
GTCGCTGAAGAATTAAACCTGTCTGTAAAAGATGTGACCGGTTTTGTTCTTGGCGGACACGGTGACGACATGGTTCCTCT
CGTCCGTTATTCCTATGCGGGCGGCATTCCGCTTGAAACGCTTATCCCGAAAGACCGTCTTGATGCGATTGTAGAACGTA
CAAGAAAAGGCGGCGGGGAAATCGTCAACCTTCTCGGCAACGGAAGCGCCTACTACGCGCCTGCTGCATCACTGACTGAA
ATGGTAGAAGCCATTTTGAAAGATCAGCGCCGCGTGCTTCCGACCATCGCTTACTTAGAAGGCGAATACGGTCATGAAGG
CATTTACTTAGGCGTACCGACGATTATCGGAGGAAACGGCCTAGAGCAAATCATCGAGCTTGAACTGACAGACTATGAAA
AAGCGCAGCTGAGTAAATCAGTTGAATCTGTCAAAAATGTGATGAAAGTATTGTCATAA

Upstream 100 bases:

>100_bases
AACTGAAGTGAAATGTTCAGAGTTTGGTGAAGAGCTAATTAAAAACATGGACTGAACCTAAAGCAGACTTCTAATGAAGC
CAAAAAAGGAGAAGAGAATC

Downstream 100 bases:

>100_bases
GAAAAAAGGGAAGGCTTCCGAATCAGAAGCCTTCCTCTTTTTACAATTAATGAAAGCGTTTTCTTTTTCCCTGAGCGATC
CTTTCTCTGAAAATGATCAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MGKTRSKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVV
ITAGIARKPGMSRDDLVSTNEKIMRSVTREIVKYSPDCIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTF
VAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
MVEAILKDQRRVLPTIAYLEGEYGHEGIYLGVPTIIGGNGLEQIIELELTDYEKAQLSKSVESVKNVMKVLS

Sequences:

>Translated_312_residues
MGKTRSKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVV
ITAGIARKPGMSRDDLVSTNEKIMRSVTREIVKYSPDCIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTF
VAEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
MVEAILKDQRRVLPTIAYLEGEYGHEGIYLGVPTIIGGNGLEQIIELELTDYEKAQLSKSVESVKNVMKVLS
>Mature_311_residues
GKTRSKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGFDAKITGTSNYEDTAGSDIVVI
TAGIARKPGMSRDDLVSTNEKIMRSVTREIVKYSPDCIIVVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFV
AEELNLSVKDVTGFVLGGHGDDMVPLVRYSYAGGIPLETLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTEM
VEAILKDQRRVLPTIAYLEGEYGHEGIYLGVPTIIGGNGLEQIIELELTDYEKAQLSKSVESVKNVMKVLS

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI47059044, Length=316, Percent_Identity=37.0253164556962, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI221136809, Length=316, Percent_Identity=37.0253164556962, Blast_Score=199, Evalue=4e-51,
Organism=Homo sapiens, GI5031857, Length=316, Percent_Identity=35.7594936708861, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI260099723, Length=316, Percent_Identity=35.7594936708861, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI291575128, Length=316, Percent_Identity=36.3924050632911, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI4557032, Length=316, Percent_Identity=36.3924050632911, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI9257228, Length=317, Percent_Identity=36.2776025236593, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI4504973, Length=317, Percent_Identity=36.2776025236593, Blast_Score=191, Evalue=6e-49,
Organism=Homo sapiens, GI15082234, Length=316, Percent_Identity=35.126582278481, Blast_Score=187, Evalue=8e-48,
Organism=Homo sapiens, GI260099727, Length=219, Percent_Identity=37.8995433789954, Blast_Score=145, Evalue=7e-35,
Organism=Homo sapiens, GI260099725, Length=200, Percent_Identity=40, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI207028494, Length=198, Percent_Identity=34.8484848484849, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI21735621, Length=324, Percent_Identity=29.320987654321, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI103472011, Length=316, Percent_Identity=26.2658227848101, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI103472015, Length=186, Percent_Identity=28.494623655914, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1789632, Length=316, Percent_Identity=30.6962025316456, Blast_Score=95, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17535107, Length=298, Percent_Identity=30.8724832214765, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI17554310, Length=320, Percent_Identity=30.3125, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322765, Length=294, Percent_Identity=29.2517006802721, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320125, Length=228, Percent_Identity=25.8771929824561, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17136226, Length=310, Percent_Identity=30.3225806451613, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI45550422, Length=303, Percent_Identity=29.3729372937294, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24647881, Length=322, Percent_Identity=30.4347826086957, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24663599, Length=308, Percent_Identity=29.2207792207792, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24663595, Length=273, Percent_Identity=29.3040293040293, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_BACA2 (A7Z7J8)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422205.1
- ProteinModelPortal:   A7Z7J8
- SMR:   A7Z7J8
- STRING:   A7Z7J8
- EnsemblBacteria:   EBBACT00000004395
- GeneID:   5463303
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_026160
- eggNOG:   COG0039
- GeneTree:   EBGT00070000032140
- HOGENOM:   HBG566126
- OMA:   YAVYKES
- ProtClustDB:   PRK06223
- BioCyc:   BAMY326423:RBAM_026160-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086
- TIGRFAMs:   TIGR01763

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 33568; Mature: 33437

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: ACT_SITE 180-180 BINDING 36-36 BINDING 87-87 BINDING 93-93 BINDING 100-100 BINDING 125-125 BINDING 156-156

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTRSKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DAKITGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTREIVKYSPDCII
CEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEE
VVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGH
EEECCCHHHHHEEEHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEHEEEEEEECC
GDDMVPLVRYSYAGGIPLETLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
CCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MVEAILKDQRRVLPTIAYLEGEYGHEGIYLGVPTIIGGNGLEQIIELELTDYEKAQLSKS
HHHHHHHHHHHHCCHHHEECCCCCCCEEEEECCCEECCCCHHHHHEECCCCCHHHHHHHH
VESVKNVMKVLS
HHHHHHHHHHHC
>Mature Secondary Structure 
GKTRSKVSVIGAGFTGATTAFLTAQKELADVVLVDIPQLENPTKGKALDMLEASPVQGF
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCEEEEECCCCCCCCCCCHHHHHCCCCCCCC
DAKITGTSNYEDTAGSDIVVITAGIARKPGMSRDDLVSTNEKIMRSVTREIVKYSPDCII
CEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEE
VVLTNPVDAMTYAVYKESGFPKERVIGQSGVLDTARFRTFVAEELNLSVKDVTGFVLGGH
EEECCCHHHHHEEEHHCCCCCHHHHCCCCCCCHHHHHHHHHHHHHCCEEEHEEEEEEECC
GDDMVPLVRYSYAGGIPLETLIPKDRLDAIVERTRKGGGEIVNLLGNGSAYYAPAASLTE
CCCCHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEECCCCEEECCHHHHHH
MVEAILKDQRRVLPTIAYLEGEYGHEGIYLGVPTIIGGNGLEQIIELELTDYEKAQLSKS
HHHHHHHHHHHHCCHHHEECCCCCCCEEEEECCCEECCCCHHHHHEECCCCCHHHHHHHH
VESVKNVMKVLS
HHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA