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The map label for this gene is dnaB [H]

Identifier: 154687031

GI number: 154687031

Start: 2726836

End: 2728245

Strand: Reverse

Name: dnaB [H]

Synonym: RBAM_026030

Alternate gene names: 154687031

Gene position: 2728245-2726836 (Counterclockwise)

Preceding gene: 154687032

Following gene: 154687030

Centisome position: 69.62

GC content: 47.66

Gene sequence:

>1410_bases
ATGGCTGACTATTGGAATGACGTATTGCCTGTAGATCCTTATACGGTCAAAAGCCGTTCGCTTCTTCAGGATGTCGACAG
ACAGGTTTTGACACATCTGTACCAGCCGCTGATCGGTTCTTTTGCATTCTCCCTTTATATGACCTTATGGGGGGAGCTTG
AGCAGAACCGCTTGTGGGGAGCGGAATCCACGCACAGGCAGCTGATGGGCATGATGCAGTCAAATTTAAAAAAGATTCAT
CATGAACAGGGAAAGCTTGAAGGAATCGGGCTGCTGAAAGTGTATATCCGGGAGTCCGAGCAGAAAGAGCGGATGTTTAT
TTATGAGCTGCTGCCCCCACTCAGACCGAATGATTTTTTTGAAGACGGCATGCTGAATGTCTTTCTTTATAACAGAGTCG
GTAAAACGAAATACCAGCAGCTGAGACAATTTTACACGCATCCTTCCGTCGGCGCGGGCGCAAGGGAAATTACCCGTCCG
TTTAATCACGCCTTTGCCTCTTTTCAGCCGAGTGAATGGAAAATGACGCCTGATATGGAAGAGGCGATGAAGCTCCCCGA
GGACTATGAGACGGCGTCTGTCGGACAAGCGCCTTCCTATACGGTGACGGAGGAAGTATTTGATTTTGAGCTTTTCATGG
CGGGACTGTCAGAAACGATGATACCGCGTAAATCAATCACGCAGGACGTGCGGGAGAGCATTAAAAAGCTTTCATATTTA
TACGGAATAGATCCTATCTCCATGCAGCACGTCGTCATGACCGCAATTAATGAACAGGATACGATAACGGCCGAAGCGCT
GAGGAAAGCGGCCAGCGACTGGTACCAAATTGAAAGAAGCGGTAAGCTTCCGGAACTGATTGATAAAACACAGCCTGTCA
CCTTGAGAGAAAACCGGACGGAAAAGGACGATTCATTGAACGGCGAACTCATCGCACAGCTTGAGACCATATCACCGAGA
AAGCTTCTTCAGGACATTGCGGACGGGACCGAGCCGTCAAAAGCCGACTTGCAGATTATCGCCGATATTATGTTCGAACA
AAAGCTTGAGCCCGGCGTCACCAATGTGCTGATTTATTATGTCATGCTGAAAACGGATATGAAGCTGTCCAAAAACTATA
TCCAAAAAATCGCCGCCCACTGGGCCAGAAAAAAAGTCAAAACGGTACGGGAAGCAATGAAGCTCGCAATAGAAGAGCAC
AGGCAATATCTTGATTGGGCGGACGGCAAAACAAACGGCGCGGCGCGCGGTAAAAAAACCGTCCGGGAAGAAAAGCGCCC
TGATTGGATGAAAGAAGAAGAAACCGGGAAGAAACAAGAACAGCCGGCTCTTTCCGCGCAGGATCTTGAAGAACAGAAGA
AAAAAATGATGGAAGAAATGAAAAAACTGAAAAAATACTCTGCCTATTGA

Upstream 100 bases:

>100_bases
GATTGTGTCTGGCTCTTTTCTCATGAGCAAAATTGGCATTTGATAGATCGCAGAAACGGTGTAAAATGAGAGTAGAAACA
TATCGGAAGGTTTGAGAATC

Downstream 100 bases:

>100_bases
GACAGAGAAGCGGGGTGATATTGAATGGAACCGATCAGCCGATCCTTAGAAGGCGTGACGAAAAGACCTGACTTTCAAAA
ACGCCTGGAACGAATGCAGC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MADYWNDVLPVDPYTVKSRSLLQDVDRQVLTHLYQPLIGSFAFSLYMTLWGELEQNRLWGAESTHRQLMGMMQSNLKKIH
HEQGKLEGIGLLKVYIRESEQKERMFIYELLPPLRPNDFFEDGMLNVFLYNRVGKTKYQQLRQFYTHPSVGAGAREITRP
FNHAFASFQPSEWKMTPDMEEAMKLPEDYETASVGQAPSYTVTEEVFDFELFMAGLSETMIPRKSITQDVRESIKKLSYL
YGIDPISMQHVVMTAINEQDTITAEALRKAASDWYQIERSGKLPELIDKTQPVTLRENRTEKDDSLNGELIAQLETISPR
KLLQDIADGTEPSKADLQIIADIMFEQKLEPGVTNVLIYYVMLKTDMKLSKNYIQKIAAHWARKKVKTVREAMKLAIEEH
RQYLDWADGKTNGAARGKKTVREEKRPDWMKEEETGKKQEQPALSAQDLEEQKKKMMEEMKKLKKYSAY

Sequences:

>Translated_469_residues
MADYWNDVLPVDPYTVKSRSLLQDVDRQVLTHLYQPLIGSFAFSLYMTLWGELEQNRLWGAESTHRQLMGMMQSNLKKIH
HEQGKLEGIGLLKVYIRESEQKERMFIYELLPPLRPNDFFEDGMLNVFLYNRVGKTKYQQLRQFYTHPSVGAGAREITRP
FNHAFASFQPSEWKMTPDMEEAMKLPEDYETASVGQAPSYTVTEEVFDFELFMAGLSETMIPRKSITQDVRESIKKLSYL
YGIDPISMQHVVMTAINEQDTITAEALRKAASDWYQIERSGKLPELIDKTQPVTLRENRTEKDDSLNGELIAQLETISPR
KLLQDIADGTEPSKADLQIIADIMFEQKLEPGVTNVLIYYVMLKTDMKLSKNYIQKIAAHWARKKVKTVREAMKLAIEEH
RQYLDWADGKTNGAARGKKTVREEKRPDWMKEEETGKKQEQPALSAQDLEEQKKKMMEEMKKLKKYSAY
>Mature_468_residues
ADYWNDVLPVDPYTVKSRSLLQDVDRQVLTHLYQPLIGSFAFSLYMTLWGELEQNRLWGAESTHRQLMGMMQSNLKKIHH
EQGKLEGIGLLKVYIRESEQKERMFIYELLPPLRPNDFFEDGMLNVFLYNRVGKTKYQQLRQFYTHPSVGAGAREITRPF
NHAFASFQPSEWKMTPDMEEAMKLPEDYETASVGQAPSYTVTEEVFDFELFMAGLSETMIPRKSITQDVRESIKKLSYLY
GIDPISMQHVVMTAINEQDTITAEALRKAASDWYQIERSGKLPELIDKTQPVTLRENRTEKDDSLNGELIAQLETISPRK
LLQDIADGTEPSKADLQIIADIMFEQKLEPGVTNVLIYYVMLKTDMKLSKNYIQKIAAHWARKKVKTVREAMKLAIEEHR
QYLDWADGKTNGAARGKKTVREEKRPDWMKEEETGKKQEQPALSAQDLEEQKKKMMEEMKKLKKYSAY

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 [H]

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006343 [H]

Pfam domain/function: PF07261 DnaB_2 [H]

EC number: NA

Molecular weight: Translated: 54465; Mature: 54334

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCCCCCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADYWNDVLPVDPYTVKSRSLLQDVDRQVLTHLYQPLIGSFAFSLYMTLWGELEQNRLWG
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AESTHRQLMGMMQSNLKKIHHEQGKLEGIGLLKVYIRESEQKERMFIYELLPPLRPNDFF
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCH
EDGMLNVFLYNRVGKTKYQQLRQFYTHPSVGAGAREITRPFNHAFASFQPSEWKMTPDME
HCCCCEEHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHH
EAMKLPEDYETASVGQAPSYTVTEEVFDFELFMAGLSETMIPRKSITQDVRESIKKLSYL
HHHCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YGIDPISMQHVVMTAINEQDTITAEALRKAASDWYQIERSGKLPELIDKTQPVTLRENRT
HCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCEEECCCCC
EKDDSLNGELIAQLETISPRKLLQDIADGTEPSKADLQIIADIMFEQKLEPGVTNVLIYY
CCCCCCCCHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHH
VMLKTDMKLSKNYIQKIAAHWARKKVKTVREAMKLAIEEHRQYLDWADGKTNGAARGKKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
VREEKRPDWMKEEETGKKQEQPALSAQDLEEQKKKMMEEMKKLKKYSAY
HHHHCCCCCCCHHHCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377 [H]