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The map label for this gene is ysbB [H]

Identifier: 154687022

GI number: 154687022

Start: 2717174

End: 2717857

Strand: Reverse

Name: ysbB [H]

Synonym: RBAM_025940

Alternate gene names: 154687022

Gene position: 2717857-2717174 (Counterclockwise)

Preceding gene: 154687023

Following gene: 154687021

Centisome position: 69.36

GC content: 47.81

Gene sequence:

>684_bases
ATGAGTCCATATTTCGGTATTGTCGTTTCACTTGCGGCCTTTGGCATCGGCACGTATTTATTTAAAAAAACGAGAGGCTT
TTTCCTCTTCACCCCGCTGTTTGTCGCGATGGTGCTTGGAATCCTTTTTCTGAAGCTCGGCGGTTTTTCATATGAAGATT
ATAAAAGCGGGGGAGACATTATTAAATTCTTCCTGGAGCCCGCGACGATCGCTTTTGCGATCCCTTTATACAAACAGCGT
GACAAACTGAAAAAATACTGGTGGCAGATCATGTCTTCTATCATTGTCGGCTCTATCTGCTCAGTCACGATTGTCTATTT
GATCGCAAAAGGCATTCATCTTGACGCGGCCGTTATGAAAAGCATGCTTCCGCAGGCGGCGACAACGGCAATTGCCCTTC
CGCTGTCAAAAGGAATCGGCGGCATCACCGACATCACCGCTTTCGCCGTTATTTTCAACGCCGTTATCGTCTATGCTATG
GGCGCTCTATTCTTAAAATTGTTTAAAGTGAAACACCCGATCTCTAAAGGACTGGCTCTCGGCACATCAGGACACGCACT
CGGTGTTGCGGTCGGTATTGAGATGGGAGAAGTAGAAGCGGCAATGGCAAGTATCGCGGTTGTCGTCGTAGGAGTGGTGA
CGGTGCTGGTTATTCCGGTATTCGTTCAGCTCATCGGAGGATAA

Upstream 100 bases:

>100_bases
GTCTGTCAGCCAAAAACGTCAGAAGGCAGCCCCTGAAAAACAAACGGTGCGCCGTTCCAATCAGGAAAAAGAACTTGTGC
AGCATTAGGAGGGAAATCGG

Downstream 100 bases:

>100_bases
ACCCGGGTCATAGACCCGGGTTTTTTTATTGTGTTGGCACGTCCGCGGGCGGCAAAGAACGAAATTTCACATTTTTCCTT
GCGCTCGCCCCGGCATCGTG

Product: antiholin-like protein LrgB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MSPYFGIVVSLAAFGIGTYLFKKTRGFFLFTPLFVAMVLGILFLKLGGFSYEDYKSGGDIIKFFLEPATIAFAIPLYKQR
DKLKKYWWQIMSSIIVGSICSVTIVYLIAKGIHLDAAVMKSMLPQAATTAIALPLSKGIGGITDITAFAVIFNAVIVYAM
GALFLKLFKVKHPISKGLALGTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG

Sequences:

>Translated_227_residues
MSPYFGIVVSLAAFGIGTYLFKKTRGFFLFTPLFVAMVLGILFLKLGGFSYEDYKSGGDIIKFFLEPATIAFAIPLYKQR
DKLKKYWWQIMSSIIVGSICSVTIVYLIAKGIHLDAAVMKSMLPQAATTAIALPLSKGIGGITDITAFAVIFNAVIVYAM
GALFLKLFKVKHPISKGLALGTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG
>Mature_226_residues
SPYFGIVVSLAAFGIGTYLFKKTRGFFLFTPLFVAMVLGILFLKLGGFSYEDYKSGGDIIKFFLEPATIAFAIPLYKQRD
KLKKYWWQIMSSIIVGSICSVTIVYLIAKGIHLDAAVMKSMLPQAATTAIALPLSKGIGGITDITAFAVIFNAVIVYAMG
ALFLKLFKVKHPISKGLALGTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG

Specific function: Inhibits the expression or activity of extracellular murein hydrolases by interacting, possibly with LrgA, with the holin-like protein CidA. The LrgAB and CidA proteins may affect the proton motive force of the membrane. May be involved in programmed cell

COG id: COG1346

COG function: function code M; Putative effector of murein hydrolase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CidB/LrgB family. LrgB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788464, Length=199, Percent_Identity=35.1758793969849, Blast_Score=125, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007300 [H]

Pfam domain/function: PF04172 LrgB [H]

EC number: NA

Molecular weight: Translated: 24164; Mature: 24032

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPYFGIVVSLAAFGIGTYLFKKTRGFFLFTPLFVAMVLGILFLKLGGFSYEDYKSGGDI
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHH
IKFFLEPATIAFAIPLYKQRDKLKKYWWQIMSSIIVGSICSVTIVYLIAKGIHLDAAVMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SMLPQAATTAIALPLSKGIGGITDITAFAVIFNAVIVYAMGALFLKLFKVKHPISKGLAL
HHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCC
GTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG
CCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPYFGIVVSLAAFGIGTYLFKKTRGFFLFTPLFVAMVLGILFLKLGGFSYEDYKSGGDI
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHH
IKFFLEPATIAFAIPLYKQRDKLKKYWWQIMSSIIVGSICSVTIVYLIAKGIHLDAAVMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
SMLPQAATTAIALPLSKGIGGITDITAFAVIFNAVIVYAMGALFLKLFKVKHPISKGLAL
HHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCC
GTSGHALGVAVGIEMGEVEAAMASIAVVVVGVVTVLVIPVFVQLIGG
CCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]