Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 154686943

GI number: 154686943

Start: 2629769

End: 2631061

Strand: Reverse

Name: folC [H]

Synonym: RBAM_025130

Alternate gene names: 154686943

Gene position: 2631061-2629769 (Counterclockwise)

Preceding gene: 154686944

Following gene: 154686942

Centisome position: 67.14

GC content: 47.72

Gene sequence:

>1293_bases
TTGTTTAATACGTACAAAGAAGCGATCAGCTGGATTCACGGCAGACTCAAGTTCGGCGTTAAGCCGGGCCTTCTCCGTAT
GGAAAAGCTGATGGAAAAACTGGAACACCCGGAAAAGAACATTCGCGCCATTCATGTGGCGGGCACAAACGGCAAAGGAT
CGACTGTCGCTTTTATCCGTTCCGTGCTTCAAGAATCCGGTTATACGGTCGGCACGTTCACATCGCCTTACATTATTACG
TTTAATGAGCGTATCAGCGTCAACGGCACGCCGATTTCTGATGAGGAATGGAGAACGCTCGTTAATGACATAAAACCGAT
AGTCGATGAGCTTGATAAGACCGATGCCGGAACGGTGACAGAATTTGAAATCATTACAGCGTGTGCATTTTTATATTTTG
CGAAGCGGGCTGATATCGATTTTGTCATTTTTGAAGCGGGGCTCGGCGGCACATTTGATTCAACCAATATTGTGAATCCG
CTCCTGTCGGTCATTACAAGTATCGGACATGACCATATGGCGATTTTAGGCAATACCATCGAGGAGATCGCCGGGCAGAA
AGCCGGTATCATTAAAAATTCCATTCCGGTTATTACGGGCGTCAATCAGCCGGAAGCACTCGGCGTTATTGAAGCGGAAG
CCGAAAAAAAGCAGGCTCCGTATCAGTCACTATATAAAACGTGCAGACTGTTTAATGAAGCGGCGCTTCCGTCCGGTGAA
AGCTTTTCGCTGGAAACGGAAACGAAATGCTATGAAAACATTCAAACGTCGCTGATCGGCATTCACCAAAGGCAGAATGC
GGCATTGGCGATATTGGCCGCTGAATGGGTGAATCGTCTGGGAGCCGCAGCCATTTCTGAAGAAGCGATGCGAAACGGCC
TGAAAAAAGCATCGTGGCCGGGCCGGTTTGAACGTCTTCAAAGCCGCCCTTTAGTGTTTGCGGACGGCGCGCATAACGAA
GAGGGCGTTGAAACGCTGACAGAAACGATCCGCCAGCGCTTTGCCGGTAAAAACGTCCATGTCGTATTCAGCGCATTAAA
AGACAAACCGTATCAGGAAATGATTAAAAAGCTTGAAACCGAAGCGGATTCCATTCACTTTGTATCATTTGACTTTCCGC
GTGCTTCAAGCGCCGAAGCCCTTTATAACGCAAGCAGTCTAGCGGATAAGAGCTTCGATGAGAACCCGGACAGTGTGAAA
GAGTGGATTGAACAAAAAGATGGTGCGGATGATGTCATCATCATTACCGGCTCCTTATACTTTATTTCAGAAATGAGAAA
GCGGCTTATCTGA

Upstream 100 bases:

>100_bases
CGCGAAGCTGTCCGGCAGCGGATTGCAGAGCTGAAAGGCTGATAAAACAGCGGGGCGGTTTCCTAAGAAACCGCCTTGTC
GTATAAAAGAGGGGATTTCA

Downstream 100 bases:

>100_bases
CAGCCCCTCGGATGCCCGACGGACCATTTTCCGGTTTTGCCGGGTGTTTTTATGCTGAAAATATATTTTCAGCCATTTTA
GTCATGGTGTTTTTCTTTGC

Product: hypothetical protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MFNTYKEAISWIHGRLKFGVKPGLLRMEKLMEKLEHPEKNIRAIHVAGTNGKGSTVAFIRSVLQESGYTVGTFTSPYIIT
FNERISVNGTPISDEEWRTLVNDIKPIVDELDKTDAGTVTEFEIITACAFLYFAKRADIDFVIFEAGLGGTFDSTNIVNP
LLSVITSIGHDHMAILGNTIEEIAGQKAGIIKNSIPVITGVNQPEALGVIEAEAEKKQAPYQSLYKTCRLFNEAALPSGE
SFSLETETKCYENIQTSLIGIHQRQNAALAILAAEWVNRLGAAAISEEAMRNGLKKASWPGRFERLQSRPLVFADGAHNE
EGVETLTETIRQRFAGKNVHVVFSALKDKPYQEMIKKLETEADSIHFVSFDFPRASSAEALYNASSLADKSFDENPDSVK
EWIEQKDGADDVIIITGSLYFISEMRKRLI

Sequences:

>Translated_430_residues
MFNTYKEAISWIHGRLKFGVKPGLLRMEKLMEKLEHPEKNIRAIHVAGTNGKGSTVAFIRSVLQESGYTVGTFTSPYIIT
FNERISVNGTPISDEEWRTLVNDIKPIVDELDKTDAGTVTEFEIITACAFLYFAKRADIDFVIFEAGLGGTFDSTNIVNP
LLSVITSIGHDHMAILGNTIEEIAGQKAGIIKNSIPVITGVNQPEALGVIEAEAEKKQAPYQSLYKTCRLFNEAALPSGE
SFSLETETKCYENIQTSLIGIHQRQNAALAILAAEWVNRLGAAAISEEAMRNGLKKASWPGRFERLQSRPLVFADGAHNE
EGVETLTETIRQRFAGKNVHVVFSALKDKPYQEMIKKLETEADSIHFVSFDFPRASSAEALYNASSLADKSFDENPDSVK
EWIEQKDGADDVIIITGSLYFISEMRKRLI
>Mature_430_residues
MFNTYKEAISWIHGRLKFGVKPGLLRMEKLMEKLEHPEKNIRAIHVAGTNGKGSTVAFIRSVLQESGYTVGTFTSPYIIT
FNERISVNGTPISDEEWRTLVNDIKPIVDELDKTDAGTVTEFEIITACAFLYFAKRADIDFVIFEAGLGGTFDSTNIVNP
LLSVITSIGHDHMAILGNTIEEIAGQKAGIIKNSIPVITGVNQPEALGVIEAEAEKKQAPYQSLYKTCRLFNEAALPSGE
SFSLETETKCYENIQTSLIGIHQRQNAALAILAAEWVNRLGAAAISEEAMRNGLKKASWPGRFERLQSRPLVFADGAHNE
EGVETLTETIRQRFAGKNVHVVFSALKDKPYQEMIKKLETEADSIHFVSFDFPRASSAEALYNASSLADKSFDENPDSVK
EWIEQKDGADDVIIITGSLYFISEMRKRLI

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=358, Percent_Identity=33.5195530726257, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI66932990, Length=358, Percent_Identity=33.5195530726257, Blast_Score=161, Evalue=1e-39,
Organism=Escherichia coli, GI1788654, Length=380, Percent_Identity=31.0526315789474, Blast_Score=146, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553150, Length=417, Percent_Identity=27.5779376498801, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=417, Percent_Identity=27.5779376498801, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553148, Length=417, Percent_Identity=27.5779376498801, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=368, Percent_Identity=29.3478260869565, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324815, Length=309, Percent_Identity=30.42071197411, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322718, Length=295, Percent_Identity=24.406779661017, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24641571, Length=294, Percent_Identity=34.3537414965986, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24581568, Length=179, Percent_Identity=32.9608938547486, Blast_Score=97, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47704; Mature: 47704

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNTYKEAISWIHGRLKFGVKPGLLRMEKLMEKLEHPEKNIRAIHVAGTNGKGSTVAFIR
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
SVLQESGYTVGTFTSPYIITFNERISVNGTPISDEEWRTLVNDIKPIVDELDKTDAGTVT
HHHHHCCCEEEEECCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
EFEIITACAFLYFAKRADIDFVIFEAGLGGTFDSTNIVNPLLSVITSIGHDHMAILGNTI
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
EEIAGQKAGIIKNSIPVITGVNQPEALGVIEAEAEKKQAPYQSLYKTCRLFNEAALPSGE
HHHCCCCCCCCCCCCCEEECCCCCCCCEEEECHHHHHCCCHHHHHHHHHHHHHHCCCCCC
SFSLETETKCYENIQTSLIGIHQRQNAALAILAAEWVNRLGAAAISEEAMRNGLKKASWP
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GRFERLQSRPLVFADGAHNEEGVETLTETIRQRFAGKNVHVVFSALKDKPYQEMIKKLET
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHHHHHHC
EADSIHFVSFDFPRASSAEALYNASSLADKSFDENPDSVKEWIEQKDGADDVIIITGSLY
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCHH
FISEMRKRLI
HHHHHHHHCC
>Mature Secondary Structure
MFNTYKEAISWIHGRLKFGVKPGLLRMEKLMEKLEHPEKNIRAIHVAGTNGKGSTVAFIR
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHH
SVLQESGYTVGTFTSPYIITFNERISVNGTPISDEEWRTLVNDIKPIVDELDKTDAGTVT
HHHHHCCCEEEEECCCEEEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCH
EFEIITACAFLYFAKRADIDFVIFEAGLGGTFDSTNIVNPLLSVITSIGHDHMAILGNTI
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHH
EEIAGQKAGIIKNSIPVITGVNQPEALGVIEAEAEKKQAPYQSLYKTCRLFNEAALPSGE
HHHCCCCCCCCCCCCCEEECCCCCCCCEEEECHHHHHCCCHHHHHHHHHHHHHHCCCCCC
SFSLETETKCYENIQTSLIGIHQRQNAALAILAAEWVNRLGAAAISEEAMRNGLKKASWP
CCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
GRFERLQSRPLVFADGAHNEEGVETLTETIRQRFAGKNVHVVFSALKDKPYQEMIKKLET
HHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEHHHHHHCCCHHHHHHHHHC
EADSIHFVSFDFPRASSAEALYNASSLADKSFDENPDSVKEWIEQKDGADDVIIITGSLY
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCHH
FISEMRKRLI
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]