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The map label for this gene is aspS

Identifier: 154686896

GI number: 154686896

Start: 2585443

End: 2587221

Strand: Reverse

Name: aspS

Synonym: RBAM_024660

Alternate gene names: 154686896

Gene position: 2587221-2585443 (Counterclockwise)

Preceding gene: 154686897

Following gene: 154686895

Centisome position: 66.02

GC content: 50.82

Gene sequence:

>1779_bases
ATGTTTGGAAGAACATATTATTGTGGCGAGATAACTGAAAAAGCGATTGGCGAAACCGTCAAACTGAAAGGATGGGTGCA
GAAGCGGCGCGATCTCGGCGGCTTGATTTTCATTGATTTGCGCGACCGCTCGGGTATTGTGCAAGTCGTGTTCAACCCTG
ACGTATCGAAGGAAGCCCTTGCCGCAGCGGAAGCAATCAGAAACGAATATGTGCTTGATATCGAAGGCACTGTTTCTGCC
CGTGAAGAAGGCACGGTGAATCCCAACCTGAAAACCGGTAAAATTGAAATCAAAGCTGAAGCGGTGACGGTGCTCAGTAC
GGCGAAAACGCCTCCGTTTCCGATCAGTGACCAGGCGGCTGACGTTTCTGAAGACGTCCGGTTAAAACACCGCTATTTGG
ATTTGCGCCGCCCGGCGATGTTTCAAACATTGCAAATGCGCCACCAAGTCACAAAATCCATCCGCCATTTCTTGGATGAA
AACGGATTTCTTGATATTGAAACACCGATTTTAACAAAAAGCTCTCCCGAAGGCGCCCGTGATTACTTAGTGCCGAGCCG
GGTGCATGAAGGAGAATTTTACGCCCTTCCGCAATCGCCGCAGATTTTTAAACAGCTTCTGATGGTGTCGGGCTTTGAAC
GGTATTATCAAGTGGCCCGCTGCTTCCGCGATGAAGACTTGCGTGCCGACCGCCAGCCGGAATTTACGCAGATTGATATC
GAAATGTCCTTTATGAGTCAAGAGGACATCATGGCTCTGGCGGAAGAGATGATGGCGAAAGTCATGCTTGAAACAAAAGG
TGTTGAAGTACCGCTTCCGCTTCCGCGAATGACATATGAAGAAGCGATGAACAGCTACGGTTCCGACAAGCCTGACACCC
GCTTCGGCATGCTGCTGACGGACGTGTCAGACATAGTGAAAGATACTGAATTTAAGGTGTTCTCATCAGCCGTGGCAAAC
GGCGGCGTTGTAAAAGCGATCAATGTCAAAGGCGCTGCCGGCGACTATTCCAGAAAAGATATCGATGCGCTCGGCGTTTT
TGCCGGAAATTACGGCGCGAAAGGTCTCGCTTGGGTGAAAGTTGAAGCCGACGGCGTAAAAGGCCCGATTGCGAAATTCT
TTGATGAAGAGAAGCAGGCAAAACTGGCTGAAGCGCTTGAAGCTTCTGAAGGCGATCTGCTCCTGTTCGGCGCTGATAAG
TTTGAGGTTGTGGCTCAATCCCTCGGCGCGCTTCGCTTAAAGCTCGGCAAAGAACGCGGCCTGATTGACGAAAGCCTCTT
CAACTTCCTGTGGGTTGTGGACTGGCCGTTACTGGAGCATGACGAGGAGGAAGGCCGTTTCTATGCGGCTCACCATCCGT
TTACAATGCCTGTCCGTGAAGATCTTGACCTGATTGAAACGGCGCCTGAAGATATGAAAGCGCAGGCTTACGACCTTGTC
TTAAACGGCTATGAACTCGGCGGAGGCTCAATCCGTATTTTTGAAAAAGACGTTCAGGAAAAAATGTTCGGGCTTCTCGG
CTTTACAAAAGAAGAAGCGGAGGAGGAGTTCGGATACCTTCTTGAAGCCTTTGAATACGGCGCCCCTCCGCACGGCGGAA
TCGCGCTCGGCTTAGACCGTTTGGTTATGCTTCTTGCGGGCCGCACAAACTTAAGAGATACGATCGCTTTCCCGAAAACG
GCAAGCGCAAGCTGCCTTCTTACGGAAGCGCCCGGCGAAGTGAGCGACGCCCAGCTTGAAGAACTTCACCTTTCTGTCAA
AAGAAAAGTGAAACAGTAA

Upstream 100 bases:

>100_bases
ATGTAAAAGATGCGGAAACAGGAGAGCAGCGTGAAGTGGTGTTAGACGAGCTGATACAAGTGTTAAAGGCAGATCAAAAG
CAGTGAAGGAGAGGAACGCT

Downstream 100 bases:

>100_bases
AGATCAGCAGTTTGTTTCTTGAAAATCAAAATCTGCTGTGATATGATTTTGACAAGTTAAATAAAGTCCTGATGTGTTAG
TTGTACACCTAGTTTTGACC

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 592; Mature: 592

Protein sequence:

>592_residues
MFGRTYYCGEITEKAIGETVKLKGWVQKRRDLGGLIFIDLRDRSGIVQVVFNPDVSKEALAAAEAIRNEYVLDIEGTVSA
REEGTVNPNLKTGKIEIKAEAVTVLSTAKTPPFPISDQAADVSEDVRLKHRYLDLRRPAMFQTLQMRHQVTKSIRHFLDE
NGFLDIETPILTKSSPEGARDYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFERYYQVARCFRDEDLRADRQPEFTQIDI
EMSFMSQEDIMALAEEMMAKVMLETKGVEVPLPLPRMTYEEAMNSYGSDKPDTRFGMLLTDVSDIVKDTEFKVFSSAVAN
GGVVKAINVKGAAGDYSRKDIDALGVFAGNYGAKGLAWVKVEADGVKGPIAKFFDEEKQAKLAEALEASEGDLLLFGADK
FEVVAQSLGALRLKLGKERGLIDESLFNFLWVVDWPLLEHDEEEGRFYAAHHPFTMPVREDLDLIETAPEDMKAQAYDLV
LNGYELGGGSIRIFEKDVQEKMFGLLGFTKEEAEEEFGYLLEAFEYGAPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKT
ASASCLLTEAPGEVSDAQLEELHLSVKRKVKQ

Sequences:

>Translated_592_residues
MFGRTYYCGEITEKAIGETVKLKGWVQKRRDLGGLIFIDLRDRSGIVQVVFNPDVSKEALAAAEAIRNEYVLDIEGTVSA
REEGTVNPNLKTGKIEIKAEAVTVLSTAKTPPFPISDQAADVSEDVRLKHRYLDLRRPAMFQTLQMRHQVTKSIRHFLDE
NGFLDIETPILTKSSPEGARDYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFERYYQVARCFRDEDLRADRQPEFTQIDI
EMSFMSQEDIMALAEEMMAKVMLETKGVEVPLPLPRMTYEEAMNSYGSDKPDTRFGMLLTDVSDIVKDTEFKVFSSAVAN
GGVVKAINVKGAAGDYSRKDIDALGVFAGNYGAKGLAWVKVEADGVKGPIAKFFDEEKQAKLAEALEASEGDLLLFGADK
FEVVAQSLGALRLKLGKERGLIDESLFNFLWVVDWPLLEHDEEEGRFYAAHHPFTMPVREDLDLIETAPEDMKAQAYDLV
LNGYELGGGSIRIFEKDVQEKMFGLLGFTKEEAEEEFGYLLEAFEYGAPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKT
ASASCLLTEAPGEVSDAQLEELHLSVKRKVKQ
>Mature_592_residues
MFGRTYYCGEITEKAIGETVKLKGWVQKRRDLGGLIFIDLRDRSGIVQVVFNPDVSKEALAAAEAIRNEYVLDIEGTVSA
REEGTVNPNLKTGKIEIKAEAVTVLSTAKTPPFPISDQAADVSEDVRLKHRYLDLRRPAMFQTLQMRHQVTKSIRHFLDE
NGFLDIETPILTKSSPEGARDYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFERYYQVARCFRDEDLRADRQPEFTQIDI
EMSFMSQEDIMALAEEMMAKVMLETKGVEVPLPLPRMTYEEAMNSYGSDKPDTRFGMLLTDVSDIVKDTEFKVFSSAVAN
GGVVKAINVKGAAGDYSRKDIDALGVFAGNYGAKGLAWVKVEADGVKGPIAKFFDEEKQAKLAEALEASEGDLLLFGADK
FEVVAQSLGALRLKLGKERGLIDESLFNFLWVVDWPLLEHDEEEGRFYAAHHPFTMPVREDLDLIETAPEDMKAQAYDLV
LNGYELGGGSIRIFEKDVQEKMFGLLGFTKEEAEEEFGYLLEAFEYGAPPHGGIALGLDRLVMLLAGRTNLRDTIAFPKT
ASASCLLTEAPGEVSDAQLEELHLSVKRKVKQ

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=598, Percent_Identity=43.4782608695652, Blast_Score=458, Evalue=1e-129,
Organism=Homo sapiens, GI5031815, Length=261, Percent_Identity=28.3524904214559, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI194272210, Length=261, Percent_Identity=28.3524904214559, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI45439306, Length=251, Percent_Identity=27.4900398406374, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI226491502, Length=258, Percent_Identity=26.3565891472868, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI4758762, Length=257, Percent_Identity=25.6809338521401, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788173, Length=595, Percent_Identity=51.9327731092437, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1790571, Length=290, Percent_Identity=26.8965517241379, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789256, Length=296, Percent_Identity=26.0135135135135, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1787161, Length=274, Percent_Identity=26.6423357664234, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI32566633, Length=604, Percent_Identity=36.0927152317881, Blast_Score=338, Evalue=6e-93,
Organism=Caenorhabditis elegans, GI17551876, Length=311, Percent_Identity=26.3665594855305, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71994340, Length=264, Percent_Identity=23.8636363636364, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17535927, Length=272, Percent_Identity=23.8970588235294, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=272, Percent_Identity=23.8970588235294, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6325153, Length=631, Percent_Identity=34.0729001584786, Blast_Score=305, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6321807, Length=248, Percent_Identity=26.6129032258064, Blast_Score=87, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=246, Percent_Identity=26.8292682926829, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=143, Percent_Identity=27.972027972028, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=147, Percent_Identity=27.2108843537415, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24584738, Length=597, Percent_Identity=41.5410385259631, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17136276, Length=244, Percent_Identity=31.5573770491803, Blast_Score=102, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24640849, Length=274, Percent_Identity=27.007299270073, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24640851, Length=274, Percent_Identity=27.007299270073, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921528, Length=232, Percent_Identity=27.1551724137931, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_BACA2 (A7Z750)

Other databases:

- EMBL:   CP000560
- RefSeq:   YP_001422057.1
- ProteinModelPortal:   A7Z750
- SMR:   A7Z750
- STRING:   A7Z750
- EnsemblBacteria:   EBBACT00000007141
- GeneID:   5462061
- GenomeReviews:   CP000560_GR
- KEGG:   bay:RBAM_024660
- eggNOG:   COG0173
- GeneTree:   EBGT00050000000858
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- BioCyc:   BAMY326423:RBAM_024660-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66059; Mature: 66059

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGRTYYCGEITEKAIGETVKLKGWVQKRRDLGGLIFIDLRDRSGIVQVVFNPDVSKEAL
CCCCEEECCHHHHHHCCCEEEHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHH
AAAEAIRNEYVLDIEGTVSAREEGTVNPNLKTGKIEIKAEAVTVLSTAKTPPFPISDQAA
HHHHHHCCCEEEEECCCCCCCCCCCCCCCCEECEEEEEEEEEEEEECCCCCCCCCCCCCC
DVSEDVRLKHRYLDLRRPAMFQTLQMRHQVTKSIRHFLDENGFLDIETPILTKSSPEGAR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCH
DYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFERYYQVARCFRDEDLRADRQPEFTQIDI
HCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFMSQEDIMALAEEMMAKVMLETKGVEVPLPLPRMTYEEAMNSYGSDKPDTRFGMLLT
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
DVSDIVKDTEFKVFSSAVANGGVVKAINVKGAAGDYSRKDIDALGVFAGNYGAKGLAWVK
HHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHEEEECCCCCCCEEEEE
VEADGVKGPIAKFFDEEKQAKLAEALEASEGDLLLFGADKFEVVAQSLGALRLKLGKERG
EECCCCCCHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
LIDESLFNFLWVVDWPLLEHDEEEGRFYAAHHPFTMPVREDLDLIETAPEDMKAQAYDLV
CHHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCEECCHHHCCHHHHCCCHHHHHHHEEEE
LNGYELGGGSIRIFEKDVQEKMFGLLGFTKEEAEEEFGYLLEAFEYGAPPHGGIALGLDR
EECEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LVMLLAGRTNLRDTIAFPKTASASCLLTEAPGEVSDAQLEELHLSVKRKVKQ
HHHHHHCCCCCCHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFGRTYYCGEITEKAIGETVKLKGWVQKRRDLGGLIFIDLRDRSGIVQVVFNPDVSKEAL
CCCCEEECCHHHHHHCCCEEEHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCHHHH
AAAEAIRNEYVLDIEGTVSAREEGTVNPNLKTGKIEIKAEAVTVLSTAKTPPFPISDQAA
HHHHHHCCCEEEEECCCCCCCCCCCCCCCCEECEEEEEEEEEEEEECCCCCCCCCCCCCC
DVSEDVRLKHRYLDLRRPAMFQTLQMRHQVTKSIRHFLDENGFLDIETPILTKSSPEGAR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCH
DYLVPSRVHEGEFYALPQSPQIFKQLLMVSGFERYYQVARCFRDEDLRADRQPEFTQIDI
HCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
EMSFMSQEDIMALAEEMMAKVMLETKGVEVPLPLPRMTYEEAMNSYGSDKPDTRFGMLLT
EEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHH
DVSDIVKDTEFKVFSSAVANGGVVKAINVKGAAGDYSRKDIDALGVFAGNYGAKGLAWVK
HHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHEEEECCCCCCCEEEEE
VEADGVKGPIAKFFDEEKQAKLAEALEASEGDLLLFGADKFEVVAQSLGALRLKLGKERG
EECCCCCCHHHHHHCHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCC
LIDESLFNFLWVVDWPLLEHDEEEGRFYAAHHPFTMPVREDLDLIETAPEDMKAQAYDLV
CHHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCEECCHHHCCHHHHCCCHHHHHHHEEEE
LNGYELGGGSIRIFEKDVQEKMFGLLGFTKEEAEEEFGYLLEAFEYGAPPHGGIALGLDR
EECEEECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
LVMLLAGRTNLRDTIAFPKTASASCLLTEAPGEVSDAQLEELHLSVKRKVKQ
HHHHHHCCCCCCHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA