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The map label for this gene is ybjI [H]

Identifier: 154686846

GI number: 154686846

Start: 2532688

End: 2533491

Strand: Reverse

Name: ybjI [H]

Synonym: RBAM_024160

Alternate gene names: 154686846

Gene position: 2533491-2532688 (Counterclockwise)

Preceding gene: 154686847

Following gene: 154686845

Centisome position: 64.65

GC content: 40.05

Gene sequence:

>804_bases
ATGATTAAATTAGTGATTACGGATTTAGACGGCACATTTTTAAATAATCAAGGCGATTTTGACAGAACGTTATTTGAAAA
GACAAAACGAGTAATGGAGGAACAGCATGTCGCTTTCGCCATCTGCACCGGGAAGCAGTGTGAAAGAGTGGAAGCTTTAT
TCGGAGAAGATGCGAAAGATTTTTGGATTTTAGGAGACAGTGCCACACGTATAAAAAAGAACGGCAAGTTCGTATATGAA
TCTTTGATCAGCAATGAAGCGGGACTGTCAATTATAGGCGCTTTAGAAAAAATCAGCTGCGGGCATAGCATTATCGCATG
CACAAAAAATGGCGCGATCGTAAAAGAAAATCTGTCTGAAAGTGAGATGAAATATGTAAATGCCTCTTATGCACATGTAA
CGAAAATAACTGATTTTACTGAGATAAAAGATGATTTTATCAAGATTACGGTTCATGATCCCGACGCCAACTGTCCGGAA
ACGAGAGAAAAGCTGCATGAATTTTTTCACCAATTATATATCGTAGCATCAGAGGATACCTGGATGGACATTGCAAATAT
CGGAATACATAAAGGAACAACAGTACAAGAACTGCAGCGATTATTAGGGGTTTCGGCTGATGAAACAATGGCGTTCGGAG
ACGGCCGCAATGACAAAGAATTGATGGAAGCGGCATCCTGCAGCTTTGCGGTTAAGAATGCCATTGACGAAATAAAAGAT
GCAGCGCACTTTGTCATCAAATCAAATGAGGAAAGCAGCGTCATGCACACGATTATGAGAATATTAGCGCTGCAAAAACA
ATGA

Upstream 100 bases:

>100_bases
CAAACAATGAAGAAGGGGTCTTAAATGCGATTGATTCCATCCTCAAATGTGAAAAACCCTTTGTCTGAAAAAGACATTTT
AACTGCGGAGGTGCTGAACG

Downstream 100 bases:

>100_bases
TGGGCTAAAATCAATGCGGCAGGACTTACTAGGGATAGAAAAAGCGGGCTATCCTCAGACTTTAATTCACTCCTGATTTT
CCTAAAGTATGTTAAAAATG

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MIKLVITDLDGTFLNNQGDFDRTLFEKTKRVMEEQHVAFAICTGKQCERVEALFGEDAKDFWILGDSATRIKKNGKFVYE
SLISNEAGLSIIGALEKISCGHSIIACTKNGAIVKENLSESEMKYVNASYAHVTKITDFTEIKDDFIKITVHDPDANCPE
TREKLHEFFHQLYIVASEDTWMDIANIGIHKGTTVQELQRLLGVSADETMAFGDGRNDKELMEAASCSFAVKNAIDEIKD
AAHFVIKSNEESSVMHTIMRILALQKQ

Sequences:

>Translated_267_residues
MIKLVITDLDGTFLNNQGDFDRTLFEKTKRVMEEQHVAFAICTGKQCERVEALFGEDAKDFWILGDSATRIKKNGKFVYE
SLISNEAGLSIIGALEKISCGHSIIACTKNGAIVKENLSESEMKYVNASYAHVTKITDFTEIKDDFIKITVHDPDANCPE
TREKLHEFFHQLYIVASEDTWMDIANIGIHKGTTVQELQRLLGVSADETMAFGDGRNDKELMEAASCSFAVKNAIDEIKD
AAHFVIKSNEESSVMHTIMRILALQKQ
>Mature_267_residues
MIKLVITDLDGTFLNNQGDFDRTLFEKTKRVMEEQHVAFAICTGKQCERVEALFGEDAKDFWILGDSATRIKKNGKFVYE
SLISNEAGLSIIGALEKISCGHSIIACTKNGAIVKENLSESEMKYVNASYAHVTKITDFTEIKDDFIKITVHDPDANCPE
TREKLHEFFHQLYIVASEDTWMDIANIGIHKGTTVQELQRLLGVSADETMAFGDGRNDKELMEAASCSFAVKNAIDEIKD
AAHFVIKSNEESSVMHTIMRILALQKQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=263, Percent_Identity=29.6577946768061, Blast_Score=120, Evalue=7e-29,
Organism=Escherichia coli, GI1787043, Length=262, Percent_Identity=25.1908396946565, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI2367265, Length=274, Percent_Identity=27.007299270073, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 29905; Mature: 29905

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKLVITDLDGTFLNNQGDFDRTLFEKTKRVMEEQHVAFAICTGKQCERVEALFGEDAKD
CEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCCC
FWILGDSATRIKKNGKFVYESLISNEAGLSIIGALEKISCGHSIIACTKNGAIVKENLSE
EEEECCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEECCCCH
SEMKYVNASYAHVTKITDFTEIKDDFIKITVHDPDANCPETREKLHEFFHQLYIVASEDT
HHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEECCCC
WMDIANIGIHKGTTVQELQRLLGVSADETMAFGDGRNDKELMEAASCSFAVKNAIDEIKD
EEHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAHFVIKSNEESSVMHTIMRILALQKQ
HHEEEEECCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKLVITDLDGTFLNNQGDFDRTLFEKTKRVMEEQHVAFAICTGKQCERVEALFGEDAKD
CEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCCCC
FWILGDSATRIKKNGKFVYESLISNEAGLSIIGALEKISCGHSIIACTKNGAIVKENLSE
EEEECCCHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCEEECCCCH
SEMKYVNASYAHVTKITDFTEIKDDFIKITVHDPDANCPETREKLHEFFHQLYIVASEDT
HHHHHHCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEECCCC
WMDIANIGIHKGTTVQELQRLLGVSADETMAFGDGRNDKELMEAASCSFAVKNAIDEIKD
EEHHHHCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
AAHFVIKSNEESSVMHTIMRILALQKQ
HHEEEEECCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]